Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,248,957	A→T	D764E (GAT→GAA)	infB	translation initiation factor IF‑2

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	REL606	3,248,957	0	A→T	100.0%	176.3	59	D764E (GAT→GAA)	infB	translation initiation factor IF‑2
Reads supporting (aligned to +/- strand):  new base (30/28):  ref base (1/0):  total (31/28)

AATCAGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGC  >  REL606/3248924‑3248989                                  |                                 aaTCAGGTTATAGATGACGGAGTAGTAACGCAGTTc                                >  1:1463210/1‑36 (MQ=255)  aTCAGGTTATAGATGACGGAGTAGTAACGCAGTTcc                               >  1:1091858/1‑36 (MQ=255)  aTCAGGTTATAGATGACGGAGTAGTAACGCAGTTcc                               >  1:5536101/1‑36 (MQ=255)  aTCAGGTTATAGATGACGGAGTAGTAACGCAGTTcc                               >  1:1347273/1‑36 (MQ=255)  aTCAGGTTATAGATGACGGAGTAGTAACGCAGTTcc                               <  1:163156/36‑1 (MQ=255)  aTCAGGTTATAGATGACGGAGTAGTAACGCAGTTcc                               >  1:1506822/1‑36 (MQ=255)   tCAGGTTATAGATGACGGAGTAGTAACGCAGTTCCa                              <  1:7419187/36‑1 (MQ=255)   tCAGGTTATAGATGACGGAGTAGTAACGCAGATCCa                              >  1:1546425/1‑36 (MQ=255)    cAGGTTATAGATGACGGAGTAGTAACGCAGTTTCAg                             >  1:4684921/1‑36 (MQ=25)    cAGGTTATAGATGACGGAGTAGTAACGCAGTTCCac                             >  1:1069693/1‑35 (MQ=255)    cAGGTTATAGATGACGGAGTAGTAACGCAGTTCCAg                             <  1:5932508/36‑1 (MQ=255)    cAGGTTATAGATGACGGAGTAGTAACGCAGTTCCAg                             >  1:5358836/1‑36 (MQ=255)     aGGTTATAGATGTCGGAGTAGTAACGCAGTTCCAgg                            <  1:3310802/36‑1 (MQ=25)     aGGTTATAGATGACGGAGTAGTAACGCAGTTCCAgg                            >  1:3213119/1‑36 (MQ=255)     aGGTAAAAGAAGACGGAGTAGTAACGCAGTTCCAgg                            <  1:524410/36‑1 (MQ=18)      ggTTATAGATGACGGAGTAGTAACGCAGTTCCAGGc                           >  1:2271294/1‑36 (MQ=255)      ggTTATAGATGACGGAGTAGTAACGCAGTTCCAGGc                           <  1:1153052/36‑1 (MQ=255)       gTTATAGATGACGGAGTAGTAGCGCAGTTCCAGGCt                          <  1:1482652/36‑1 (MQ=25)       gTTATAGATGACGGAGTAGTAACGCAGTTCCAGGCt                          <  1:266355/36‑1 (MQ=255)        ttATAGATGACGGAGTAGTCACGCCGTTCCAGGcct                         >  1:124373/1‑34 (MQ=18)        ttATAGATGACGGAGTAGTAACGCAGTTCCAGGCtt                         <  1:4517960/36‑1 (MQ=255)        ttATAGATGACGGAGTAGTAACGCAGTTCCAGGCtt                         <  1:4055730/36‑1 (MQ=255)         tataGATGACGGAGTAGTAACGCAGTTCCAGGCttt                        >  1:514290/1‑36 (MQ=255)          ataGATGACGGAGTAGTAACGCAGTTCCAGGGTTTc                       >  1:7198517/1‑36 (MQ=25)          ataGATGACGGAGTAGTAACGCAGTTCCACGCCTTc                       >  1:6134219/1‑36 (MQ=21)           taGATGACGGAGTAGTAACGCAGTTCCAGGCTTTcc                      <  1:775410/36‑1 (MQ=255)           taGATGACGGAGTAGTAACGCAGTTCCAGGCTTTcc                      <  1:1130055/36‑1 (MQ=255)           taGATGACGGAGTAGTAACGCAGTTCCAGGCTTTcc                      <  1:1721017/36‑1 (MQ=255)           taGATGACGGAGTAGTAACGCAGTTCCAGGCTTTcc                      <  1:5710418/36‑1 (MQ=255)            aGATGACGGAGTAGTAACGCAGTTCCAGGCTTTCCg                     >  1:1957473/1‑36 (MQ=255)             gATGACGGAGTAGTAACGCAGTTCCAGGGTTTCCGc                    >  1:6251795/1‑36 (MQ=25)              aTGACGGAGTAGTAACGCAGTTCCAGGCTTTTCggt                   >  1:495059/1‑34 (MQ=21)               tGACGGAGTAGTAACGCAGTTCCAGGCTTTCCGCtt                  <  1:4848473/36‑1 (MQ=255)               tGACGGAGTAGTAACGCAGTTCCAGGCTTTCCGCtt                  <  1:4869048/36‑1 (MQ=255)                gACGGGGTAGTAACGCAGTTCCAGGCTTTCCGCTTc                 <  1:5146035/36‑1 (MQ=25)                gACGGAGTAGTAACGCAGTTCCAGGCTTTCCGCTTc                 <  1:4124724/36‑1 (MQ=255)                gACGGAGTAGTAACGCAGTTCCAGGCTTTCCGCTTc                 >  1:1129977/1‑36 (MQ=255)                 aCGGAGTAGTAATGCAGTTCCAGGCTTTCCGCTTCa                >  1:4022201/1‑36 (MQ=25)                 aCGGAGTAGTAACGCAGTTCCAGGCTTTCCGCTTCa                <  1:4436847/36‑1 (MQ=255)                 aCGGAGTAGTAACGCAGTTCCAGGCTTTCCGCTTCa                >  1:2555069/1‑36 (MQ=255)                 aCGGAGTAGTAACGCAGTTCCAGGCTTTCCGCTTCa                <  1:2953276/36‑1 (MQ=255)                    gAGTAGTAACGCAGTTCCAGGCTTTCCGCTTCaacc             >  1:53240/1‑34 (MQ=25)                     agtagtAACGCAGTTCCAGGCTTTCCGCTTCAATCa            <  1:5529879/36‑1 (MQ=255)                      gtagtaACTCAGTTCCAGGCTTTCCGCTTCAATCAc           <  1:3968829/36‑1 (MQ=25)                       tagtaGCGCAGTTCCGGGCTTTCCGCTTCAATCACt          <  1:6634058/36‑1 (MQ=21)                       tagtaACGCAGTTCCAGGCTTTCCGCTTCATTTACt          >  1:4818997/1‑36 (MQ=21)                        agtaACGCAGTTCCAGGCTTTCCGCTTCAATCACtt         >  1:3491399/1‑36 (MQ=255)                         gtaACGCAGTTCCAGGCTTTCCGCTTCAATCACttt        >  1:5674925/1‑36 (MQ=255)                          taACGCAGTTCCAGGCTTTCCGCTTCAATCACTTTa       <  1:6294081/36‑1 (MQ=255)                           aaCGCAGTTCCAGGCTTTCCGCTTCAATCACTTTAc      >  1:4627322/1‑36 (MQ=255)                           aaCGCAGTTCCAGGCTTTCCGCTTCAATCACTTTAc      <  1:5352756/36‑1 (MQ=255)                           aaCGCAGTTCCAGGCTTTCCGCTTCAATCACTTTAc      <  1:2154822/36‑1 (MQ=255)                             cGCAGTTCCAGGCTTTCCGCTTCAATCACTTTACGt    <  1:3172413/36‑1 (MQ=255)                              gCAGTTCCAGGCTTTCCGCTTCAATCACTTTACGTg   >  1:18828/1‑36 (MQ=255)                              gCAGTTCCAGGCTCTCCGCTTCAATCACTCTACGtc   >  1:1044670/1‑35 (MQ=21)                               cAGTTCCAGGCTTTCCGCTTCAATCACTTTACGttc  >  1:3367672/1‑34 (MQ=37)                               cAGTTCCAGGCTTTCCGCTTCAATCACTTTACGTGc  >  1:2597024/1‑36 (MQ=255)                               cAGTTCCAGGCTTTCCGCTTCAATCACTTTACGTGc  >  1:1357485/1‑36 (MQ=255)                               cAGTTCCAGGCTTTCCGCTTCAATCACTTTACGTGc  <  1:1355433/36‑1 (MQ=255)                                  |                                 AATCAGGTTATAGATGACGGAGTAGTAACGCAGATCCAGGCTTTCCGCTTCAATCACTTTACGTGC  >  REL606/3248924‑3248989 Base quality scores:  ATCG  < 3 ≤  ATCG  < 9 ≤  ATCG  < 18 ≤  ATCG  < 29 ≤  ATCG  < 32 ≤  ATCG