Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,200,643	C→A	intergenic (+11/‑245)	tdcR/yhaB	DNA‑binding transcriptional activator/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	REL606	3,200,643	0	C→A	100.0%	156.2	50	intergenic (+11/‑245)	tdcR/yhaB	DNA‑binding transcriptional activator/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (35/15):  ref base (0/0):  total (35/15)

GAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAG  >  REL606/3200612‑3200675                                |                                 gAATGCAATCGTTTTCCATAAATTCTTCTCCACTCa                              >  1:5468702/1‑36 (MQ=255)  aaTGCAATCGTTTTCCATAAATTCTTCTCCACTCAt                             <  1:6399491/36‑1 (MQ=255)  aaTGCAATCGTTTTCCATAAATTCTTCTCCACTCAt                             >  1:4283999/1‑36 (MQ=255)   aTGCAATCGTTTTCCATAAATTCTTCTCCACTCATa                            >  1:6418684/1‑36 (MQ=255)    tGCAATCGTTTTCCATAAATTCTTCTCCACTCATAg                           >  1:2349925/1‑36 (MQ=255)    tGCAATCGTTTTCCATAAATACTTCTCCACTCATAg                           >  1:6356281/1‑36 (MQ=25)     gCAATCGTTTTCCATAAATTCTTCTCCACTCATAgg                          >  1:6037947/1‑36 (MQ=255)     gCAATCGTTTTCCATAAATCCTTCTCCATTCATAgg                          >  1:1947174/1‑36 (MQ=21)      cAATCGTTTTCCATAAATTCTTCTCCACTCATAGGc                         >  1:1110409/1‑36 (MQ=255)       aaTCGTTTTCCATAAATTCTTCTCCACTCATAGGcg                        >  1:4898023/1‑36 (MQ=255)       aaTCGTTTTCCATAAATTCTTCTCCACTCATAGGcg                        >  1:5567908/1‑36 (MQ=255)       aaTCGTTTTCCATAAATTCTTCTCCACTCATAAGcg                        >  1:5896180/1‑36 (MQ=21)       aaTCGTTTTCCATAAATTCTTCACCACTCATAGGcg                        >  1:1672331/1‑36 (MQ=21)        aTCGTTTTCCATAAATTCTTCTCCACTCATAGGcga                       >  1:1360165/1‑36 (MQ=255)        aTCGTTTTCCATAAATTCTTCTCCACTCATAGGcga                       <  1:4785386/36‑1 (MQ=255)        aTCGTTTTCCATAAATTCTTCTCCACTCATACGcnc                       >  1:677769/1‑34 (MQ=21)          cGTTTTCCATAAATTCTTCTCCACTCATAGGcgacg                     >  1:2374060/1‑36 (MQ=255)          cGTTTTCCATAAATTCTTCTCCACTCATAGGcgacg                     <  1:3569408/36‑1 (MQ=255)          cGTTTTCCATAAATTCTTCTCCACTCATAGGcgacg                     >  1:5392802/1‑36 (MQ=255)            ttttCCATAAATTCTTCTCCACTCATAGGCGACGaa                   <  1:5323459/36‑1 (MQ=255)            ttttCCATAAATTCTTCTCCACTCATAGGCGACGaa                   >  1:5263865/1‑36 (MQ=255)             tttCCATAAATTCTTCTCCACTCATAGGCGACGAAt                  <  1:3687787/36‑1 (MQ=255)              ttCCATAAATTCTTCTCCACTCATAGGCGACGAATa                 >  1:64283/1‑36 (MQ=255)              ttCCATAAATTCTTCTCCACTCATAGGCGACGAATa                 <  1:678329/36‑1 (MQ=255)               tCCATAAATTCTTCTCCACTCATAGGCGACGAATAg                >  1:6819206/1‑36 (MQ=255)                  aTAAATTCTTCTCCACTCATAGGCGACGAATAGCAt             <  1:3210520/36‑1 (MQ=255)                   tAAATTCTTCTCCACTCATAGGCGACGAATAGCAtt            <  1:5031278/36‑1 (MQ=255)                    aaaTACTTCTCCACTCATAGGCGACGAATAGCAttt           <  1:2880302/36‑1 (MQ=25)                     aaTTCTTCTCCACTCATAGGCGACGAATAGCAtttt          >  1:5815989/1‑36 (MQ=255)                     aaTTCTTCTCCACTCATAGGCGACGAATAGCAtttt          >  1:776190/1‑36 (MQ=255)                      aTTCTTCTCCACTCATAGGCGACGAATAGCATTTtg         >  1:2303404/1‑36 (MQ=255)                      aTTCTTCTCCACTCATAGGCGACGAATAGCATTTtg         >  1:5128280/1‑36 (MQ=255)                       ttcttcTCCACTCATAGGCGACGAATAGCATTTtgt        >  1:2729012/1‑36 (MQ=255)                       ttcttcTCCACTCATAGGCGACGAATAGCATTTtgt        <  1:1316069/36‑1 (MQ=255)                       ttcttcTCCACTCATAGGCGACGAATAGCATTTtgt        <  1:2900158/36‑1 (MQ=255)                        tcttctCCACTCATAGGCGACGAATAGCCTTTtgtg       >  1:5188395/1‑36 (MQ=21)                        tcttctCCACTCATAGGCGACGAATAGCATTTtgtg       <  1:379653/36‑1 (MQ=255)                        tcttctCCACTCATAGGCGACGAATAGCATTTtgtg       >  1:4965949/1‑36 (MQ=255)                        tcttctCCACTCATAGGCGACGAATAGCATTTtgtg       >  1:2351717/1‑36 (MQ=255)                         cttctCCACTCATAGGCGACGAATAGCATTTTgtgt      >  1:2902369/1‑36 (MQ=255)                         cttctCCACTCATAGGCGACGAATAGCATTTTgtgt      <  1:1893824/36‑1 (MQ=255)                         cttatCCACTCATAGGCGAAGAATAGCATTTTgtgt      >  1:14388/1‑36 (MQ=255)                          ttctCCACTCATAGGCGACGAATAGCATTTTGTGtt     >  1:3911840/1‑36 (MQ=255)                          ttctCCACTCATAGGCGACGAATAGAATTTTGTGtt     >  1:254762/1‑36 (MQ=25)                           tctcCACTCATAGGCGACGACTAGCATTTTGTGTTg    >  1:6747344/1‑36 (MQ=25)                            ctcCACTCATAGGCGACGAATAGCATTTTGTGttca   >  1:273122/1‑34 (MQ=25)                            ctcCACTCATAGGCGACGAATAGCATTTTGTGTTGa   <  1:3669450/36‑1 (MQ=255)                            ctcCACTCATAGGCGACGAATAGCATTTTGTGTTGa   >  1:1966418/1‑36 (MQ=255)                             tcCACTCATAGGCGACGAATAGCATTTTGTGTTGAg  >  1:3260662/1‑36 (MQ=255)                             tcCACTCATAGGCGACGAATAGCATTTTGTGTTGAg  <  1:5883660/36‑1 (MQ=255)                                |                                 GAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAG  >  REL606/3200612‑3200675 Base quality scores:  ATCG  < 3 ≤  ATCG  < 11 ≤  ATCG  < 20 ≤  ATCG  < 30 ≤  ATCG  < 32 ≤  ATCG