Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,045,069	G→T	T312N (ACC→AAC)	yghJ	predicted inner membrane lipoprotein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	REL606	3,045,069	0	G→T	100.0%	84.6	27	T312N (ACC→AAC)	yghJ	predicted inner membrane lipoprotein
Reads supporting (aligned to +/- strand):  new base (21/6):  ref base (0/0):  total (21/6)

CCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTT  >  REL606/3045037‑3045101                                 |                                 ccAGTTCAAAGGTATCGATACCAAAGGAGATGTttt                               >  1:4901898/1‑36 (MQ=255)  cAGTTCAAAGGTATCGATACCAAAGGAGATGTTTTc                              >  1:1427057/1‑36 (MQ=255)  cAGTTCAAAGGTATCGATACCAAAGGAGATGTTTTc                              <  1:3610319/36‑1 (MQ=255)   aGTTCAAAGGTATCGATACCAAAGGAGATGTTTTCg                             >  1:225670/1‑36 (MQ=255)     ttCAAAGGTATCGATACCAAAGGAGATGTTATCGcc                           >  1:4699285/1‑36 (MQ=25)      tCAAAGGTATCGATACCAAAGGAGATGTTTTCGccc                          <  1:869611/36‑1 (MQ=255)       cAAAGGTATCGATACCAAAGGAGATGTTTTCGcccc                         >  1:6271781/1‑36 (MQ=255)          aGGTAACGATACCAAAGGAGATGTTTTCGCCCCAGc                      <  1:4313516/36‑1 (MQ=25)              aTCGATACCAAAGGAGATGTTTTCGCCCCAGCTaaa                  >  1:296669/1‑36 (MQ=255)              aTCGATACCAAAGGAGATGTTTTCGCCCCAGCTaaa                  >  1:7026701/1‑36 (MQ=255)              aTCGATACCAAAGGAGATGTTTTAGCCCCAGCTaaa                  >  1:3856997/1‑36 (MQ=25)               tCGATACCAAAGGAGATGTTTTCGCCCCAGCTAAAg                 <  1:4079367/36‑1 (MQ=255)                cGATACCAAAGGAGATGTTTTCGCCCCAGCTAAAgg                >  1:5248789/1‑36 (MQ=255)                cGATACCAAAGGAGATGTTTTCGCCCCAGCTAAAgg                >  1:1503711/1‑36 (MQ=255)                cGATACCAAAGGAGATGTTTTCGCCCCAGCTAAAgg                >  1:4870217/1‑36 (MQ=255)                  aTACCAAAGGAGATGTTTTCGCCCCAGCTAAAGGaa              >  1:582933/1‑36 (MQ=255)                  aTACCAAAGGAGATGTTTTCGCCCCAGCGAAAGGaa              <  1:4361649/36‑1 (MQ=25)                    aCCAAAGGAGATGTTTTCGCCCCAGCTAAAGGaaaa            >  1:2885438/1‑36 (MQ=255)                    aCCAAAGGAGATGTTTTCGCCCCAGCTAAAGAaaaa            >  1:1287014/1‑36 (MQ=25)                     ccAAAGGAGATGTTTTCGCCCCAGCTAAAGGAAAAt           <  1:4105278/36‑1 (MQ=255)                         aGGAGATGTTTTCGCCCCAGCTAAAGGAAAATTCAc       >  1:1294933/1‑36 (MQ=255)                           gagaTGTTTTCGCCCCAGCTAAAGGAAAATTCACCa     >  1:465226/1‑36 (MQ=255)                           gagaTGTTTTCGCCCCAGCTAAAGGAAAATTCACCa     >  1:1122728/1‑36 (MQ=255)                            agaTGTTTTCGCCCCAGCTAAAGGAAAATTCACCAt    >  1:400602/1‑36 (MQ=255)                             gaTGTTTTCGCCCCAGCTAAAGGAAAATTCACCact   >  1:2738781/1‑34 (MQ=25)                             gaTGTTTTCGCCCCAGCTAAAGGAAAATTCACCAtt   >  1:2751178/1‑36 (MQ=255)                              aTGTTTTCGCCCCAGCTAAAGGAAAATCCACCAtct  >  1:2275473/1‑34 (MQ=21)                                 |                                 CCAGTTCAAAGGTATCGATACCAAAGGAGATGGTTTCGCCCCAGCTAAAGGAAAATTCACCATTT  >  REL606/3045037‑3045101 Base quality scores:  ATCG  < 3 ≤  ATCG  < 13 ≤  ATCG  < 20 ≤  ATCG  < 30 ≤  ATCG  < 32 ≤  ATCG