Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,082,685	G→A	A494V (GCT→GTT)	yegI	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	REL606	2,082,685	0	G→A	100.0%	95.2	31	A494V (GCT→GTT)	yegI	hypothetical protein
Reads supporting (aligned to +/- strand):  new base (21/10):  ref base (0/0):  total (21/10)

CGTTCCCTTGCGGTGTCGTGAAGTGCTGCCAGAGCACGTTTTTCTTCCTCCGGTAACCCGAGCATT  >  REL606/2082652‑2082717                                  |                                 cGTTCCCTTGCGGTGTCGTGAAGTGCTGCCAGAACa                                >  1:7261297/1‑36 (MQ=255) cGTTCCCTTGCGGTGTCGTGAAGTGCTGCCAGAACa                                >  1:5551688/1‑36 (MQ=255) cGTTCCCTTGCGGTGTCGTGAAGTGCTGCCAGAACa                                >  1:4635249/1‑36 (MQ=255)   ttCCCTTGCGGTGTCGTGAAGTGCTGCCAGAACACg                              >  1:6138829/1‑36 (MQ=255)   tgcccTGGCGGTGTCGTGAAGTGCTGCCAGAACACg                              <  1:1478860/34‑1 (MQ=21)    acccTTGCGGTGTCGTGAAGTGCTGCCAGAACACGt                             >  1:4701653/2‑36 (MQ=255)       cTTGCGGTGTCGTGAAGTGCTGCCAGAACACGtttt                          <  1:1304903/36‑1 (MQ=255)       cTTGCGGTGTCGTGAAGTGCTGCCAGAACACGtttt                          >  1:7443565/1‑36 (MQ=255)       cTTGCGGTGTATTGAAGTGCTGCCAGAACACGtttt                          <  1:3941910/36‑1 (MQ=21)        ttGCGGTGTCGTGAAGTGCTGCCAGAACACGttttt                         >  1:4767753/1‑36 (MQ=255)         tGCGGTGTCGTGAAGTGCTGCCAGAACACGTTTttc                        >  1:1187499/1‑36 (MQ=255)         tGCGGTGTCGTGAAGTGCTGCCAGAACACGTTTttc                        <  1:5965269/36‑1 (MQ=255)          gCGGTGTCGTGAAGTGCTGCCAGAACACGTTTttct                       >  1:3119428/1‑36 (MQ=255)           cGGTGTCGTGAAGTGCTGCCAGAACACGTTTttctt                      >  1:657407/1‑36 (MQ=255)           cGGTGTCGTGAAGTGCTGCCAGAACACGTTTttctt                      >  1:2084856/1‑36 (MQ=255)            ggTGTCGTGAAGTGCTGCCAGAACACGTTTttcttc                     >  1:4349959/1‑36 (MQ=255)             gtgtCGTGAAGTGCTGCCAGAACACGTTTTTCTtcc                    >  1:3722440/1‑36 (MQ=255)             gtgtCGTGAAGTGCTGCCAGAACACGTTTTTCTtcc                    >  1:1930372/1‑36 (MQ=255)              tgtCGTGAAGTGCTGCCAGAACACGTTTTTCTtcct                   >  1:1805251/1‑36 (MQ=255)              tgtCGTGAAGTGCTGCCAGAACACGTTTTTCTtcct                   <  1:2197183/36‑1 (MQ=255)               gtCGTGGAGTGCTGCCAGGACACGTTTTTCTtcctc                  <  1:749567/36‑1 (MQ=21)               gtCGTGAAGTGCTGCCAGAACACGTTTTTCTtcctc                  >  1:4339436/1‑36 (MQ=255)                 cGTGAAGTGCTGCCAGAACACGTTTTTCTTCCTCCg                >  1:841856/1‑36 (MQ=255)                 cGTGAAGTGCTGCCAGAACACGTTTTTCTTCCTCCg                <  1:3057066/36‑1 (MQ=255)                  gTGAAGTGCTGCCAGAACACGTTTTTCTCCCTCCgg               >  1:6763988/1‑36 (MQ=25)                   tGAAGTGCTGCCAGAACACGTTTTTCTTCCTCCGGt              >  1:1842684/1‑36 (MQ=255)                     aggTGTTGCCAGAACACGTTTTTCTTCCTCCGGTaa            <  1:4804860/34‑1 (MQ=21)                      aGTGCTGCCAGAACACGTTTTTCTTCCTCCGGTAAc           >  1:1936281/1‑36 (MQ=255)                       gTGCTGCCAGAACACGTTTTTCTTCCTCCGGTAAcc          >  1:5300603/1‑36 (MQ=255)                          ctgcCAGAACACGTTTTTCTTCCTCCGGTAACCCGa       <  1:6296707/36‑1 (MQ=255)                              cAGAACACGTTTTTCTTCCTCCGGTAACCCGAGCAt   <  1:2815179/36‑1 (MQ=255)                               agaaCACGTTTTTCTTCCTCCGGTAACCCGAGCAtt  <  1:708533/36‑1 (MQ=255)                               agaaCACGTTTTTCTTCCTCCGGTAACCCGAGCAtt  <  1:2834824/36‑1 (MQ=255)                                  |                                 CGTTCCCTTGCGGTGTCGTGAAGTGCTGCCAGAGCACGTTTTTCTTCCTCCGGTAACCCGAGCATT  >  REL606/2082652‑2082717 Base quality scores:  ATCG  < 3 ≤  ATCG  < 12 ≤  ATCG  < 19 ≤  ATCG  < 30 ≤  ATCG  < 32 ≤  ATCG