Read alignment evidence...
  seq id position change freq score reads annotation genes product
*REL60687,8120T→G35.4% 16.848V214G (GTT→GGT) leuOleucine transcriptional activator
Reads supporting (aligned to +/- strand):  new base (17/0):  ref base (8/23):  total (25/23)

GAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTT  >  REL606/87777‑87847
                                   |                                   
gtaaCATGATGTTTATAACGAACAACATGCGGCGGt                                     <  1:2338051/34‑1 (MQ=255)
gAAACATGATGTTTATAACGAACAACATGCGGCGGt                                     <  1:1107522/36‑1 (MQ=255)
 aaaCATGATGTTTATAACGAACAACATGCGGCGGtt                                    <  1:5328220/36‑1 (MQ=255)
  aaCATGATGTTTATAACGAACAACATGCGGCGGttt                                   <  1:7469250/36‑1 (MQ=255)
  aaCATGATGTTTATAACGAACAACATGCGGCGGttt                                   >  1:3397972/1‑36 (MQ=255)
   aCATGATGTTTATAACGAACAACATGCGGCGGgttt                                  >  1:432014/1‑35 (MQ=255)
   aCATGATGTTTATAACGAACAACATGCGGCGGgttg                                  >  1:6958005/1‑35 (MQ=255)
   aCATGATGTTTATAACGAACAACATGCGGCGGTTTc                                  <  1:5970235/36‑1 (MQ=255)
     atgatgTTTATAACGAACAACATGCGGCGGgtttgt                                >  1:5819296/1‑33 (MQ=21)
     atgatgTTTATAACGAACAACATGCGGCGGTTTTGc                                >  1:6224776/1‑36 (MQ=255)
     atgatgTTTATAACGAACAACATGCGGCGGTTTCGc                                <  1:4389612/36‑1 (MQ=255)
      tgatgTTTATAACGAACAACATGCGGCGGTTTCGCt                               <  1:2568066/36‑1 (MQ=255)
       gatgTTTATAACGAACAACATGCGGCGGTTTCGCTc                              <  1:3238881/36‑1 (MQ=255)
       gatgTTTATAACGAACAACATGCGGCGGTTTCGCTc                              <  1:4508180/36‑1 (MQ=255)
       gatgTTTATAACGAACAACATGCGGCGGTTTCCCTc                              >  1:4884899/1‑36 (MQ=255)
         tgTTTATAACGAACAACATGCGGCGGGTTTGCTCgt                            >  1:5287636/1‑35 (MQ=18)
          gTTTATAACGAACAACATGCGGCGGTTTCGCTCGAt                           <  1:686128/36‑1 (MQ=255)
          gTTTATAACGAACAACATACGGCGGTTTCGCTCGAt                           <  1:2870384/36‑1 (MQ=255)
           tttATAGCGAACAACATGCGGCGGTTTCGCTCGATc                          <  1:1681342/36‑1 (MQ=255)
           tttATAACGAACAACATGCGGCGGTTTCGCTCGATc                          <  1:5969954/36‑1 (MQ=255)
            ttATAACGAACAACATGCGGCGGGTTCGCTCGGTCg                         >  1:5825722/1‑36 (MQ=21)
             tataACGAACAACATGCGGCGGGTTCGCTCGCTcct                        >  1:970030/1‑34 (MQ=18)
             tataACGAACAACATGCGGCGGGTTCGCTCGATcct                        >  1:5448454/1‑34 (MQ=21)
               taACGAACAACATGCGGCGGTTTCGCTCGATCGttt                      <  1:5283482/36‑1 (MQ=255)
               taACGAACAACATGCGGCGGGTTCGCTCGATCGttt                      >  1:6056160/1‑36 (MQ=255)
                aaCGAACAACATGCGGCGGGTTTGCTTGCTCGTTTc                     >  1:1890047/1‑36 (MQ=17)
                aaCGAACAACATGCGGCGGGTTTGCTCGATCGtttt                     >  1:3411719/1‑35 (MQ=18)
                aaCGAACAACATGCGGCGGGTTCGGTCGTTTGtttt                     >  1:3193138/1‑35 (MQ=17)
                  cGAACGACATGCGGCGGTTTCGCTCGATCGTTTcgc                   <  1:6396573/36‑1 (MQ=255)
                  cGAACAACATGCGGCGGTTTCGCTCGCTCGttttgt                   >  1:1424491/1‑33 (MQ=21)
                  cGAACAACATGCGGCGGGTTTGCTCGATCCTTTcgc                   >  1:4481876/1‑36 (MQ=18)
                    aacaacATGCGGCGGGTTTGGTCGGTCGTTTCGCGt                 >  1:7044507/1‑36 (MQ=17)
                    aacaacATGCGGCGGGTTTGGTCGGTCCTTTCGCGt                 >  1:4890643/1‑36 (MQ=9)
                    aacaacATGCGGCGGGTTCGCTCCCTCGTTTcccgc                 >  1:3752689/1‑35 (MQ=17)
                     acaacaTGCGGCGGGTTTGCTCGATTGTTTCTCGTc                >  1:382669/1‑36 (MQ=18)
                     acaacaTGCGGCGGGTTCGCTCGTTCGTTTCGCGTc                >  1:2596306/1‑36 (MQ=25)
                     acaacaTGCGGCGGGTTCGCTCCATCGTTTCGCGTc                >  1:6294343/1‑36 (MQ=25)
                      caacaTGCGGCGGTTTCGCTCGATCGTTTCGCGTCa               <  1:4257048/36‑1 (MQ=255)
                      caacaTGCGGCGGGTTCGCTCGGGCGGTTCGTGTcc               >  1:7019122/1‑35 (MQ=12)
                      caacaTACGGCGGTTTCGCTCGATCGTTTCGCGTCa               <  1:6632219/36‑1 (MQ=255)
                        acaTGCGGCGGTTTCGCTCGATCGTTTCGCGTCAtt             <  1:1548556/36‑1 (MQ=255)
                         caTGCGGCGGTTTCGCTCCATCGTTTCGCGTCCttt            >  1:4326953/1‑36 (MQ=25)
                          aTGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTa           <  1:3141746/36‑1 (MQ=255)
                           tGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAg          <  1:7439546/36‑1 (MQ=255)
                            gcggcgGGTTCGCTCGATCGTTTTGCGTCCTTTatt         >  1:4334528/1‑34 (MQ=18)
                             cggcggTTTCGCTCGGTGGTTTCGCGTCATTTAGTc        <  1:7460704/36‑1 (MQ=25)
                             cggcggTTTCGCTCGATCGTTTCGCGTCATTTAGTc        <  1:7442479/36‑1 (MQ=255)
                               gcggTTTCGCTTGATCGTTTCGCGTCATTTAGTCaa      <  1:2998878/36‑1 (MQ=255)
                               gcggTTTCGCTCGATCGTTTCGCGTCATTTAGTCaa      >  1:734978/1‑36 (MQ=255)
                                cggTTTCGCTCGATCGTTTCGCGTCATTTATTCAAc     >  1:3850343/1‑36 (MQ=255)
                                cggTTTCGCTCGATCGTTTCGCGTCATTTAGTCAAc     <  1:2842226/36‑1 (MQ=255)
                                cggTTTCGCTCGATCGTTTCGCGTCATTTAGTCAAc     >  1:2854957/1‑36 (MQ=255)
                                cggTTTCGCTCGATCGTTTCGCGTCATTTAGTCAAc     <  1:1531304/36‑1 (MQ=255)
                                 ggTTTCGCTCGATCGTTTCGCGTCATTTAGTCAAcc    <  1:41125/36‑1 (MQ=255)
                                 ggTTTCGCTCGATCGTTTCGCGTCATTTAGTCAAcc    >  1:814174/1‑36 (MQ=255)
                                  gTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCt   <  1:1987751/36‑1 (MQ=255)
                                   tttCGCTCGATCGTTTCGCGTCATTTAGTCAACCtt  >  1:5240739/1‑36 (MQ=255)
                                   tttCGCTCGATCGTTTCGCGTCATTTAGTCAACCtt  >  1:3753331/1‑36 (MQ=255)
                                   |                                   
GAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTT  >  REL606/87777‑87847

Base quality scores:  ATCG  < 3 ≤  ATCG  < 5 ≤  ATCG  < 12 ≤  ATCG  < 27 ≤  ATCG  < 32 ≤  ATCG