Read alignment evidence...
  seq id position change freq score reads annotation genes product
*REL6064,582,4710T→G32.3% 10.832V527G (GTG→GGG) tsrmethyl‑accepting chemotaxis protein I, serine sensor receptor
Reads supporting (aligned to +/- strand):  new base (10/0):  ref base (1/20):  total (11/21)

CCGGATTCAGCAACAGCAGCGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAA  >  REL606/4582436‑4582505
                                   |                                  
ccGGATTCAGCAACAGCAGCGTGAAACATCGGCtgt                                    <  1:1799386/36‑1 (MQ=255)
  ggATTCAGCAACAGCAGCGTGAAACATCGGCTGGgg                                  >  1:3251198/1‑36 (MQ=255)
   gATTCAGCAACAGCAGCGTGAAACATCGGCTGGggg                                 >  1:7156960/1‑35 (MQ=255)
    aTTCAGCAACAGCAGCGTGAAACATCGGCTGGgggg                                >  1:6902777/1‑34 (MQ=25)
    aTTCAGCAACAGCAGCGTGAAACATCGGCTGGgggg                                >  1:6786499/1‑34 (MQ=25)
    aTTCAGCAACAGCAGCGTGAAACATCGGCTGGgggg                                >  1:5611801/1‑34 (MQ=25)
      tCAACAACAGCAGCGTGAAACATCGGCTGTGGTaaa                              <  1:4681114/36‑1 (MQ=255)
       cAGCAACAGCAGCGTGAAACATCGGCTGGGGGaaaa                             >  1:7027738/1‑36 (MQ=25)
        aGCAACAGCAGCGTGAAACATCGGCTGTGGTaaaaa                            <  1:4080929/36‑1 (MQ=255)
        aGCAACAGCAGCGTGAAACATCGGCTGTGGTaaaaa                            <  1:3214587/36‑1 (MQ=255)
         gCAACAGCAGCGTGAAACATCGGCTGTGGTAAAAAc                           <  1:977389/36‑1 (MQ=255)
           aaCAGCAGCGTGAAACAGCGGCTGTGGTAAAAACCg                         <  1:4774323/36‑1 (MQ=255)
            aCAGCAGGGTGAAACATCGGCTGTGGTAAAAACCGt                        <  1:3779473/36‑1 (MQ=255)
            aCAGCAGGGTGAAACACCGGCTGTGGTAAAAACCGt                        <  1:2362452/36‑1 (MQ=25)
            aCAGCAGCGTGAAACATCGGCTGTGGTAAAAACCGt                        <  1:5141346/36‑1 (MQ=255)
            aCAGCAGCGTGAAACATCGGCTGTGGTAAAAACCGt                        <  1:2573631/36‑1 (MQ=255)
            aCAGCAACGTGAAACATCGGCTGTGGTAAAAACCGt                        <  1:422727/36‑1 (MQ=255)
              agcagcGTGAAACATCGGCTGTGGTAAAAACCGTGa                      <  1:7287502/36‑1 (MQ=255)
               gcagcGTGAAACATCGGCTGTGGTAAAAACCGTGAc                     <  1:4940907/36‑1 (MQ=255)
               gcagcGTGAAACATCGGCTGGGGGGAAAAACGCGaa                     >  1:7012227/1‑35 (MQ=12)
               acagcGTAAAACATCGGCTGTGGTAAAAACCGTGAc                     <  1:2154114/35‑1 (MQ=255)
                cagcGGGAAACATCGGCTGTGGTAAAAACCGTGACg                    <  1:4939977/36‑1 (MQ=255)
                 agcGTGAAACATCGGCTGTGGTAAAAACCGTGACGc                   <  1:4587720/36‑1 (MQ=255)
                 agcGTGAAACATCGGCTGGGGGAAAAAACATGACgg                   >  1:1190980/1‑35 (MQ=18)
                  gcGTAAAACATCGGCTGTGATAAAAACCGTGACGcc                  <  1:2339842/36‑1 (MQ=25)
                    gTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAg                <  1:2132311/36‑1 (MQ=255)
                    acgAAACATCGGCTGGGGGAAAAACCGTGGCGCCac                >  1:4948486/3‑35 (MQ=17)
                     tGAAACATCGGCTGGGGGGAAAAAAGGGAGGCCAGc               >  1:3094252/1‑36 (MQ=2)
                       aaaCATCGGCTGTGGTAAAAACCGTGACGCCAGCTg             <  1:691365/36‑1 (MQ=255)
                          cATCGGCTGTGGTAAAAACCGTGACGCCAGCTgcgc          <  1:3558453/36‑1 (MQ=255)
                          cATCGGCGGTGGTAAAAACCGTGACGCCAGCTgcgc          <  1:7380677/36‑1 (MQ=255)
                           gtCGGCTGAGGTAAAAACCGTGACGCCAGCTGcgcc         <  1:3303674/35‑1 (MQ=255)
                                cTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTa    >  1:3647524/1‑36 (MQ=255)
                                  gtgGTAAAAACCGTGACGCCAGCTGCGCCGCGTaaa  <  1:2950856/36‑1 (MQ=255)
                                  gtgGTAAAAACCGTGACGCCAGCTGCGCCGCGTaaa  >  1:1916696/1‑36 (MQ=255)
                                   |                                  
CCGGATTCAGCAACAGCAGCGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAA  >  REL606/4582436‑4582505

Base quality scores:  ATCG  < 3 ≤  ATCG  < 5 ≤  ATCG  < 13 ≤  ATCG  < 26 ≤  ATCG  < 32 ≤  ATCG