Read alignment evidence...
  seq id position change freq score reads annotation genes product
*REL6064,463,0160A→T49.5% 139.8112Y85F (TAT→TTT) yjgNconserved inner membrane protein
Reads supporting (aligned to +/- strand):  new base (1/52):  ref base (25/29):  total (26/86)

Only 100 of 114 total aligned reads displayed.

TGTTTTTTTTAGTTGTC.TTGTTT.TTGTTTTTTTCTATTTCGCAATATTAATGACAGTGTCAGCAGATATGC  >  REL606/4462981‑4463051
                                     |                                   
ttgttttttgTGTTGT..TTGTTT.TTTTGTTTTTTTTt                                    <  1:4239137/33‑1 (MQ=0)
 tttttttttGTTTTTG.TTTTGT.TTGTTTTTTTTTTt                                    <  1:4238264/35‑1 (MQ=2)
 ttttttgttGGTGGTT.TTTTTT.TTTTGTTTTTTTTt                                    <  1:6924454/35‑1 (MQ=0)
 gTTTTTTTTAGTTGTC.TTGTTT.TTGTTTTTTTCTAt                                    <  1:6237629/36‑1 (MQ=255)
  ttttttttTTTTTTC.TTTAAT.TTGTTTTTTTCTTtt                                   <  1:7301756/36‑1 (MQ=2)
  ttttttttTGTTG.C.TTTTTG.TTTTTTTTTCCTGttt                                  <  1:3497517/36‑1 (MQ=0)
  ttttttttTGTTCTT.TTATTT.TTTTGTTTTTTTTtt                                   <  1:7310586/36‑1 (MQ=2)
  ttttgtttTGTT.TC.TTGTTT.TTTTTTTGTTTTTttt                                  <  1:7011359/36‑1 (MQ=2)
  tttattttTGTTTTT.TTTTGT.TTTTTTTTTTGTTtt                                   <  1:6580355/36‑1 (MQ=9)
  ttctttttGTTTTTC.TTTTTT.TTTTGTTTTTTTTtt                                   <  1:7200181/33‑1 (MQ=0)
  tgttttttA.TTCTC.TTTTTG.TTTTTTTTTTTTTttt                                  <  1:7011272/34‑1 (MQ=9)
  gtatttttATTTTTT.TTTTGT.TTTTTTTTTTTTTtt                                   <  1:7218219/33‑1 (MQ=9)
  ctttttttTTTTGT..TTATTT.TTGTTTTTTTTTTttt                                  <  1:6477284/35‑1 (MQ=2)
   tttttttTGTT.TT.TTGTTT.TT.TTTTTTTGTAttttt                                <  1:6480639/36‑3 (MQ=0)
   tttttttGTTTTTG.TTTTTT.TTGTTTTTTTGTTttt                                  <  1:6580363/36‑1 (MQ=2)
   tttttttGTTTTTG.TTGTTT.TTTTTTTGTTTTTttt                                  <  1:6490092/36‑1 (MQ=2)
   tttttttGTTTATT.TTGTTT.TTGTTTTTTTTTTttt                                  <  1:6469532/36‑1 (MQ=9)
   tttttttAGTTGTC.TTGTTT.TTGTTTTTTTTTAttt                                  >  1:7135059/1‑36 (MQ=255)
   tttttttAGTTGTC.TTGTTT.TTGTTTTTTTCTAttt                                  >  1:4579394/1‑36 (MQ=255)
   tttttttA.TTTTG.TTTTTT.TCGTTTCTTTTTTtttt                                 <  1:6992331/36‑2 (MQ=0)
   ttttttgCGTTGTT.TTTTGT.TTTTTTTTTTTTTttt                                  <  1:7393115/36‑1 (MQ=2)
   ttttgttTGTTGTT.TTTTGT.TTTTTTTTTTTTTttt                                  <  1:3862874/36‑1 (MQ=0)
   tttatttGGTTTTT.TTGTTT.TTTTTTTTTTTTTttt                                  <  1:3479249/36‑1 (MQ=9)
   ttgttttTGTTCTT.TTTTTT.TTGTTTTTTTTTTttt                                  <  1:3368036/33‑1 (MQ=2)
   tgtttttTGTTGTT.TTTTTT.TTGTTTTTTTTTTttt                                  <  1:3570334/34‑1 (MQ=2)
   gttttttTTTTTTG.TTTTTT.TCTTTTGTTTCTGttt                                  <  1:6500252/35‑1 (MQ=2)
   gttttttTTTTGTT.GTTTTT.TTGTTTTTTTTTTttt                                  <  1:6458944/35‑1 (MQ=2)
   gttttttGTTTTTT.TTGTTT.TTTTTTTATTTTTttt                                  <  1:6905235/35‑1 (MQ=2)
   gttttttGGTTTTT.TTTTTT.TTGTTTTTTTTTTttt                                  <  1:7328214/35‑1 (MQ=0)
   gttttttATTTTTT.TTGTTT.TTTTATTTTTTTTttt                                  <  1:7473958/35‑1 (MQ=0)
   gttttctAGTTG...TTGTTT.TTGTTTTTTTTTTttttt                                <  1:6924536/35‑3 (MQ=2)
   gtttattTGTTTTT.TTTTTG.TTTTTTTTTTTTTttt                                  <  1:7143998/35‑1 (MQ=0)
   cttttttTTTTTTT.TTGTTT.TTTTTTTGTTCTTttt                                  <  1:7115177/35‑1 (MQ=2)
   cttttttTGTTTTT.TTTTTT.TTGTTTTTTT.TAtttt                                 <  1:7329393/35‑2 (MQ=2)
    ttttttTTTTCTT.TTGTTT.TTTTGTTTTTTTTtgtt                                 <  1:6982431/36‑4 (MQ=0)
    ttttttTTTTCTG.TTCTTT.TTGTTTTTTTTTCtttt                                 <  1:6895488/36‑2 (MQ=0)
    ttttttTGTTTTT.TTTTTG.TTTTTTTTTTTTGtttg                                 <  1:4675564/36‑2 (MQ=2)
    ttttttTGTTTTG.TGTTTT.TTGTTTTTTTTTTtttt                                 <  1:7181282/36‑2 (MQ=2)
    ttttttTCTTGTC.TT.TTT.TTGTTTTTTTTTTttttt                                <  1:6798474/36‑3 (MQ=0)
    ttttttTCTTGCT.TTTTTT.TTTTTTGTTTTTTtttt                                 <  1:6759184/36‑2 (MQ=2)
    ttttttA.TTTTT.TTTTTT.TTGTTTTTTTTTTtttgg                                <  1:6640510/36‑3 (MQ=9)
    tttttcTGTTTTT.TTGTTT.TTTTTTCTTTTTAttgt                                 <  1:6768905/36‑3 (MQ=2)
    tttttcTGTTGTT.TTGTTT.TTTTGTTTTTTTTtttt                                 <  1:6580383/36‑2 (MQ=9)
    ttttatATTTTTT.TTGTTT.TTTGTTTTTTTTAtttt                                 <  1:6933943/36‑2 (MQ=2)
    gtttttTTTTGTT.TTATTT.TCTTTTTTGTTTTtttg                                 <  1:6924677/35‑2 (MQ=2)
    gtttttCGTTTTT.TTTTTT.TTGTTTTTTTTTTtttt                                 <  1:6520416/35‑2 (MQ=2)
    gattttTGTTTTT.TTGTTT.TTTTTTTTTTTTTtttt                                 <  1:7456279/34‑2 (MQ=9)
     tttttTGTTTTC.TT.TTT.TGTTTTTTTTTTAtttttt                               <  1:7374361/36‑4 (MQ=0)
     tttttTGTTGTT.TGTTTT.TTGTGTTTTTGTTttttt                                <  1:6699914/36‑3 (MQ=2)
     tttttTGGTGTT.TTTTTT.TTTTTGTTTTTTTtttgt                                <  1:3942153/36‑3 (MQ=0)
     tttttTCTTGTC.TTTTTG.TTTTTTTTTTTTTttttt                                <  1:6680134/36‑3 (MQ=0)
     tttttCTTTGTCTTTGTTT.TTTTTTTGTTTTTtttt                                 <  1:6529126/36‑2 (MQ=0)
     tttttATTTGTT.GTTTTT.TTGTTTTTTTTTTttttt                                <  1:7039604/36‑3 (MQ=2)
     tttttAGTTGTC.TTGTTT.TTGTTTTTTTCTTTTTct                                >  1:4688369/1‑35 (MQ=255)
     tttttAGTTGTC.TTGTTT.TTGTTTTTTTCTATTTct                                >  1:2475464/1‑35 (MQ=255)
     tttttAGTTGTC.TTGTTT.TTGTTTTTTTCTATTTct                                >  1:7049367/1‑35 (MQ=255)
     gttttAATTTTT.TTTTTG.TTTTTTTTTTTTTTTTct                                <  1:6837370/35‑2 (MQ=9)
      ttttTGTTTCC.TTTTTT.TTGTTTTTTTTTTtttttt                               <  1:7320093/36‑4 (MQ=2)
      ttttTGTTGTTTTTGTTT.TTTTTTTTTTATTttttt                                <  1:6600332/36‑3 (MQ=2)
      ttttTGTTGGT.TTTTTG.TTTTTTTTTTTTTtttttt                               <  1:7365647/36‑4 (MQ=2)
      ttttTGTTCTT.TTTTTT.TTTTGTTTTTTTTTTTctt                               <  1:7255717/36‑3 (MQ=2)
      ttttAGTTGTC.TTGTTT.TTGTTTTTTTCTATTTCGc                               <  1:257317/36‑1 (MQ=255)
       tttCGTTGTT.TTTTTTGTTTTTTTTTTGTTtttttt                               <  1:6953814/36‑4 (MQ=2)
       tttAGTTGTC.TTGTTT.TTGTTTTTTTCTATTTCGCa                              <  1:5024875/36‑1 (MQ=255)
       tttAGTTGTC.TTGTTT.TTGTTTTTTTCTATTTCGCa                              >  1:6137428/1‑36 (MQ=255)
        ttAGTTGTC.TTGTTT.TTGTTTTTTTCTATTTCTCaa                             >  1:7385047/1‑36 (MQ=255)
         tAGTTGTC.TTGTTT.TTGTTTTTTTCTATTTCGCAat                            >  1:4872804/1‑36 (MQ=255)
         tAGTTGTC.TTGTTT.TTGTTTTTTTCTATTTCGCAat                            <  1:4099861/36‑1 (MQ=255)
          aGTTGTC.TTGTTT.TTGTTTTTTTCTATTTCTCAata                           >  1:7178448/1‑36 (MQ=255)
          aGTTGTC.TTGTTT.TTGTTTTTTTCTATTTCGCAata                           <  1:3901407/36‑1 (MQ=255)
           gTTGTC.TTGTTT.TTGTTTTTTTCTATTTCGCAatat                          <  1:2821673/36‑1 (MQ=255)
           gTTGTC.TTGTTT.TTGTTTTTTTCTATTTCGCAatat                          <  1:4540145/36‑1 (MQ=255)
            ttGTC.TTGTTT.TTGTTTGTTTCTATTTCGCAATAtt                         <  1:6422616/36‑1 (MQ=255)
             tGTC.TTGTTT.TTGTTTTTTTCTATTTCGCAATATTa                        <  1:154748/36‑1 (MQ=255)
             tGTC.TTGTTT.TTGTTTTTTTCTATTTCGCAATATTa                        >  1:347989/1‑36 (MQ=255)
             tGTC.TTGTTT.TTGTTTTTTTCTATTTCGCAATATTa                        <  1:610406/36‑1 (MQ=255)
             tGTC.TTGTTT.TTGTTTTTTTCTATTTCGCAATATTa                        <  1:6840805/36‑1 (MQ=255)
              gTC.TTGTTT.TTGTTTTTTTCTATTTCGCAATATTaa                       >  1:4409435/1‑36 (MQ=255)
               tC.TTGTTT.TTGTTTTTTTCTATTTCGCAATATTAAt                      <  1:956878/36‑1 (MQ=255)
               tC.TTGTTT.TTGTTTTTTTCTATTTCGCAATATTAAt                      >  1:3607742/1‑36 (MQ=255)
                c.TTGTTT.TTGTTTTTTTCTATTTCGCAATATTAATg                     <  1:5295601/36‑1 (MQ=255)
                c.TTGTTT.TTGTTTTTTTCTATTTCGCAATATTAATg                     >  1:4665578/1‑36 (MQ=255)
                c.TTGTTT.TTGTTTTTTTCTATTTCGCAATATTAATg                     >  1:3386959/1‑36 (MQ=255)
                c.TTGTTT.TTGTTTTTTTATATTTAGAAATATTTATg                     >  1:3385393/1‑36 (MQ=18)
                  ttgttt.ttgtttTTTTCTATTTCGCAATATTAATGa                    <  1:3779418/36‑1 (MQ=255)
                   tgttt.ttgttttTTTCTATTTCGCAATATTAATGAc                   <  1:328005/36‑1 (MQ=255)
                     ttt.ttttttttTTTTATTTCGCAATATTAATGACAg                 >  1:6993050/1‑36 (MQ=25)
                     ttt.ttgtttttTTCTATTTCGCAATATTAATGACAg                 >  1:80790/1‑36 (MQ=255)
                      tt.ttgtttttTTCTATTTCGCAATATTAATGACAgt                >  1:2075596/1‑36 (MQ=255)
                      tt.ttgtttttTTCTATTTCGCAATATTAATGACAgt                <  1:1571200/36‑1 (MQ=255)
                       t.ttgtttttTTCTATTTCGCAATATTAATGACTgtg               >  1:5479975/1‑36 (MQ=255)
                       t.ttgtttttTTCTATTTCGCAATATTAATGACAgtg               >  1:2967728/1‑36 (MQ=255)
                         ttgtttttTTCTATTTCGTAATATTAATGACAgtgt              >  1:2442516/1‑36 (MQ=255)
                          tgtttttTTCTATTTCGCAATATTAATGACAGTGTc             <  1:2933530/36‑1 (MQ=255)
                            tttttttCTATTTCGCAATATTAATGACAGTGTcag           >  1:6811203/1‑36 (MQ=255)
                              tttttCTATTTCGCAATATTAATGACAGTGTcagca         <  1:2265981/36‑1 (MQ=255)
                              tttttCTAGTTCGCAATATTAATGACAGTGTcagca         <  1:3634158/36‑1 (MQ=255)
                                 ttCTATTTCGCATTATTAATGACAGTGTCAGCAGat      <  1:3147568/36‑1 (MQ=255)
                                    caTTTCGCAATATTAATGACAGTGTCAGCGGATATg   >  1:5455150/2‑36 (MQ=255)
                                     aTTTCGCAATATTAATGACAGTGTCAGCAGatattc  >  1:3850553/1‑34 (MQ=255)
                                     |                                   
TGTTTTTTTTAGTTGTC.TTGTTT.TTGTTTTTTTCTATTTCGCAATATTAATGACAGTGTCAGCAGATATGC  >  REL606/4462981‑4463051

Base quality scores:  ATCG  < 3 ≤  ATCG  < 5 ≤  ATCG  < 10 ≤  ATCG  < 22 ≤  ATCG  < 32 ≤  ATCG