Read alignment evidence...
  seq id position change freq score reads annotation genes product
*REL6064,463,0140C→T44.4% 116.8127F84F (TTC→TTTyjgNconserved inner membrane protein
Reads supporting (aligned to +/- strand):  new base (2/50):  ref base (26/39):  total (29/98)

Only 100 of 132 total aligned reads displayed.

AATGTTTTTTTTAGTTG.TC.TTG.TTT.T.TGTTTT.TTTCTATTTCGCAATATTAATGACAGTGTCAGCAGATA  >  REL606/4462979‑4463048
                                         |                                  
tttGTTTCTTTTATTTA.T..TTT.TTG.T.TTTTTT.TTTTt                                   <  1:6620494/34‑1 (MQ=12)
tttGTCTTTTTTTTTTT.TG.TTT.TTT.T.TTTTTT.TTTc                                    <  1:6866358/34‑1 (MQ=9)
tctGTTTTTTCTTTTTT.TT.TTT.TTT.T.TGTTTT.TTTc                                    <  1:2720065/34‑1 (MQ=2)
tcggTTTTTTTGTGTTT.TT.TGT.TTT.T.TGTTTT.TTTc                                    <  1:3561262/33‑1 (MQ=2)
gaTGTTTTTTTTAGTTG.TC.TTG.TTT.T.TGTTTT.TTTc                                    <  1:834183/35‑1 (MQ=255)
 ttGTGTTTTTTTTTTT.TG.TTT.TTT.T.TTTTTT.TTTGt                                   <  1:7115022/35‑1 (MQ=9)
 aTGTTTTTTTTAGTTG.TC.TTG.TTT.T.TGTTTT.TTTCt                                   >  1:1883715/1‑36 (MQ=255)
 aTGTTTTTTTTAGTTG.TC.TTG.TTT.T.TGTTTT.TTTCt                                   <  1:2552385/36‑1 (MQ=255)
  ttgttttttgTGTTG.T..TTG.TTT.T.TTTGTT.TTTTTTt                                 <  1:4239137/33‑1 (MQ=0)
  tcttttttttGTTTT.TT.TTG.TTT.T.TTTTTTATTTCt                                   <  1:7237157/34‑1 (MQ=0)
  tGTTTTTTTTTGTT..TT.TTG.TTT.T.TTTTTT.TTTCttt                                 <  1:6787830/36‑3 (MQ=9)
  tGTTTTGTTTTGTT..TT.TTG.TTT.T.T.TTTT.TTTCTTtt                                <  1:7373668/36‑1 (MQ=2)
  gtttttttttGTTTT.TG.TTT.TTT.T.TTTTTT.TTTGtt                                  <  1:3461035/34‑2 (MQ=2)
   tttttttttGTTTT.TG.TTT.TTT.TCTCTTTT.TTTCtt                                  <  1:3026018/35‑2 (MQ=2)
   tttttttttAGTTG.TC.TTG.TTT.T.TGTTTT.TTTCttt                                 >  1:7467831/2‑34 (MQ=255)
   ttttttttgTGTTGTTG.TTT.TTT.T.TGTTTT.TTTCtt                                  <  1:7320185/35‑2 (MQ=2)
   tttttttctTTTTG.TT.TTGTTTT.T.TGTTTT.TTTCtt                                  <  1:7030189/35‑2 (MQ=0)
   ttttttgttGGTGG.TT.TTT.TTT.T.TTTGTT.TTTTTTt                                 <  1:6924454/35‑1 (MQ=0)
   gTTTTTTTTAGTTG.TC.TTG.TTT.T.TGTTTT.TTTCttt                                 >  1:311559/1‑34 (MQ=255)
   gTTTTTTTTAGTTG.TC.TTG.TTT.T.TGTTTT.TTTCTAt                                 <  1:6237629/36‑1 (MQ=255)
   gTTTTTTGTTTTTT.TG.TTT.TTT.T.TTTTTT.TTGCttt                                 <  1:7320080/36‑3 (MQ=2)
    ttttttttTTTTT.TC.TTT.AAT.T.TGTTTT.TTTCTTtt                                <  1:7301756/36‑1 (MQ=2)
    ttttttttTGTTG..C.TTT.TTG.T.TTTTTT.TTCCTGttt                               <  1:3497517/36‑1 (MQ=0)
    ttttttttTGTTC.TT.TTA.TTT.T.TTTGTT.TTTTTTtt                                <  1:7310586/36‑1 (MQ=2)
    tttattttTGTTT.TT.TTT.TGT.T.TTTTTT.TTTGTTtt                                <  1:6580355/36‑1 (MQ=9)
    ttctttttGTTTT.TC.TTT.TTT.T.TTTGTT.TTTTTTtt                                <  1:7200181/33‑1 (MQ=0)
    tgttttttA.TTT.TC.TTG..TC.T.TGTTTT.TCTTTCTTTc                              <  1:6856852/34‑1 (MQ=9)
    tgttttttA.TTC.TC.TTT.TTG.T.TTTTTT.TTTTTTttt                               <  1:7011272/34‑1 (MQ=9)
     tttttttTTTTG..C.TTT.TTT.T.TTTTTT.TTTCTAtttt                              <  1:6973006/36‑2 (MQ=9)
     tttttttTGTT..TT.TTG.TTT.T.T.TTTT.TTTGTAttttt                             <  1:6480639/36‑3 (MQ=0)
     tttttttGTTTT.TG.TTG.TTT.T.TTTTTT.GTTTTTttt                               <  1:6490092/36‑1 (MQ=2)
     tttttttGTTTA.TT.TTG.TTT.T.TGTTTT.TTTTTTttt                               <  1:6469532/36‑1 (MQ=9)
     tttttttAGTTG.TC.TTG.TTT.T.TGTTTT.TTTTTAttt                               >  1:7135059/1‑36 (MQ=255)
     tttttttA.TTT.TG.TTT.TTT.T.CGTTTC.TTTTTTtttt                              <  1:6992331/36‑2 (MQ=0)
     ttttttgCGTTG.TT.TTT.TGT.T.TTTTTT.TTTTTTttt                               <  1:7393115/36‑1 (MQ=2)
     ttttgttTGTTG.TT.TTT.TGT.T.TTTTTT.TTTTTTttt                               <  1:3862874/36‑1 (MQ=0)
     tttatttGGTTT.TT.TTG.TTT.T.TTTTTT.TTTTTTttt                               <  1:3479249/36‑1 (MQ=9)
     ttgttttTGTTC.TT.TTT.TTT.T.TGTTTT.TTTTTTttt                               <  1:3368036/33‑1 (MQ=2)
     tgtttttTGTTG.TT.TTT.TTT.T.TGTTTT.TTTTTTttt                               <  1:3570334/34‑1 (MQ=2)
     gttttttTTTTT.TG.TTT.TTT.T.CTTTTG.TTTCTGttt                               <  1:6500252/35‑1 (MQ=2)
     gttttttTTTTG.TT.GTT.TTT.T.TGTTTT.TTTTTTttt                               <  1:6458944/35‑1 (MQ=2)
     gttttttGTTTT.TT.TTG.TTT.T.TTTTTT.ATTTTTttt                               <  1:6905235/35‑1 (MQ=2)
     gttttttGGTTT.TT.TTT.TTT.T.TGTTTT.TTTTTTttt                               <  1:7328214/35‑1 (MQ=0)
     gttttctAGTTG....TTG.TTT.T.TGTTTT.TTTTTTttttt                             <  1:6924536/35‑3 (MQ=2)
     gtttattTGTTT.TT.TTT.TTG.T.TTTTTT.TTTTTTttt                               <  1:7143998/35‑1 (MQ=0)
     cttttttTTTTT.TT.TTG.TTT.T.TTTTTT.GTTCTTttt                               <  1:7115177/35‑1 (MQ=2)
     cttttttTGTTT.TT.TTT.TTT.T.TGTTTT.TTT.TAtttt                              <  1:7329393/35‑2 (MQ=2)
      ttttttTTTTG.TT.TTT.TTG.T.TTTTTT.TCTTTTTTTc                              <  1:7237196/36‑1 (MQ=2)
      ttttttTGTTT.TT.TTT.TTG.T.TTTTTT.TTTTTGtttg                              <  1:4675564/36‑2 (MQ=2)
      ttttttTGTTT.TG.TGT.TTT.T.TGTTTT.TTTTTTtttt                              <  1:7181282/36‑2 (MQ=2)
      ttttttTCTTG.TC.TT..TTT.T.TGTTTT.TTTTTTttttt                             <  1:6798474/36‑3 (MQ=0)
      ttttttTCTTG.CT.TTT.TTT.T.TTTTTG.TTTTTTtttt                              <  1:6759184/36‑2 (MQ=2)
      ttttttA.TTT.TT.TTT.TTT.T.TGTTTT.TTTTTTtttgg                             <  1:6640510/36‑3 (MQ=9)
      tttttcTGTTG.TT.TTG.TTT.T.TTTGTT.TTTTTTtttt                              <  1:6580383/36‑2 (MQ=9)
      ttttatATTTT.TT.TTG.TTT.T.TTGTTT.TTTTTAtttt                              <  1:6933943/36‑2 (MQ=2)
      gtttttCGTTT.TT.TTT.TTT.T.TGTTTT.TTTTTTtttt                              <  1:6520416/35‑2 (MQ=2)
      gattttTGTTT.TT.TTG.TTT.T.TTTTTT.TTTTTTtttt                              <  1:7456279/34‑2 (MQ=9)
       tttttTGTTT.TC.TT..TTT.T.GTTTTT.TTTTTAtttttt                            <  1:7374361/36‑4 (MQ=0)
       tttttTGTTG.TT.TGT.TTT.T.TGTGTT.TTTGTTttttt                             <  1:6699914/36‑3 (MQ=2)
       tttttTGGTG.TT.TTT.TTT.T.TTTTGT.TTTTTTtttgt                             <  1:3942153/36‑3 (MQ=0)
       tttttGGTTG.TT.TTT.GTT.T.TCTTTT.TTCCTTttttt                             <  1:6689322/36‑3 (MQ=0)
       tttttCTTTG.TCTTTG.TTT.T.TTTTTT.GTTTTTtttt                              <  1:6529126/36‑2 (MQ=0)
       tttttATTTG.TT.GTT.TTT.T.TGTTTT.TTTTTTttttt                             <  1:7039604/36‑3 (MQ=2)
       tttttAGTTG.TC.TTG.TTT.T.TGTTTT.TTTCTTTTTct                             >  1:4688369/1‑35 (MQ=255)
       tttttAGTTG.TC.TTG.TTT.T.TGTTTT.TTTCTATTTct                             >  1:2475464/1‑35 (MQ=255)
       tttttAGTTG.TC.TTG.TTT.T.TGTTTT.TTTCTATTTct                             >  1:7049367/1‑35 (MQ=255)
        ttttTGTTG.TTTTTG.TTT.T.TTTTTT.TTTATTttttt                             <  1:6600332/36‑3 (MQ=2)
        ttttTGTTG.GT.TTT.TTG.T.TTTTTT.TTTTTTtttttt                            <  1:7365647/36‑4 (MQ=2)
        ttttTGTTC.TT.TTT.TTT.T.TTTGTT.TTTTTTTTTctt                            <  1:7255717/36‑3 (MQ=2)
         tttCGTTG.TT.TTT.TTTGT.TTTTTT.TTTGTTtttttt                            <  1:6953814/36‑4 (MQ=2)
         tttAGTTG.TC.TTG.TTT.T.TGTTTT.TTTCTATTTCGCa                           >  1:6137428/1‑36 (MQ=255)
         tttAGTTG.TC.TTG.TTT.T.TGTTTT.TTTCTATTTCGCa                           <  1:5024875/36‑1 (MQ=255)
           tAGTTG.TC.TTG.TTT.T.TGTTTT.TTTCTATTTCGCAat                         <  1:4099861/36‑1 (MQ=255)
            aGTTG.TC.TTG.TTT.T.TGTTTT.TTTCTATTTCTCAata                        >  1:7178448/1‑36 (MQ=255)
            aGTTG.TC.TTG.TTT.T.TGTTTT.TTTCTATTTCGCAata                        <  1:3901407/36‑1 (MQ=255)
             gTTG.TC.TTG.TTT.T.TGTTTT.TTTCTATTTCGCAatat                       <  1:4540145/36‑1 (MQ=255)
             gTTG.TC.TTG.TTT.T.TGTTTT.TTTCTATTTCGCAatat                       <  1:2821673/36‑1 (MQ=255)
              ttG.TC.TTG.TTT.T.TGTTTG.TTTCTATTTCGCAATAtt                      <  1:6422616/36‑1 (MQ=255)
               tG.TC.TTG.TTT.T.TGTTTT.TTTCTATTTCGCAATATTa                     <  1:6840805/36‑1 (MQ=255)
               tG.TC.TTG.TTT.T.TGTTTT.TTTCTATTTCGCAATATTa                     <  1:154748/36‑1 (MQ=255)
               tG.TC.TTG.TTT.T.TGTTTT.TTTCTATTTCGCAATATTa                     <  1:610406/36‑1 (MQ=255)
                g.TC.TTG.TTT.T.TGTTTT.TTTCTATTTCGCAATATTaa                    >  1:4409435/1‑36 (MQ=255)
                  tC.TTG.TTT.T.TGTTTT.TTTCTATTTCGCAATATTAAt                   <  1:956878/36‑1 (MQ=255)
                  tC.TTG.TTT.T.TGTTTT.TTTCTATTTCGCAATATTAAt                   >  1:3794360/1‑36 (MQ=255)
                   c.TTG.TTT.T.TGTTTT.TTTCTATTTCGCAATATTAATg                  <  1:5295601/36‑1 (MQ=255)
                   c.TTG.TTT.T.TGTTTT.TTTCTATTTCGCAATATTAATg                  >  1:4665578/1‑36 (MQ=255)
                   c.TTG.TTT.T.TGTTTT.TTTATATTTAGAAATATTTATg                  >  1:3385393/1‑36 (MQ=18)
                     ttg.ttt.t.tgtttT.TTTCTATTTCGCAATATTAATGa                 <  1:3779418/36‑1 (MQ=255)
                      tg.ttt.t.tgtttt.TTTCTATTTCGCAATATTAATGAc                >  1:2864677/1‑36 (MQ=255)
                      tg.ttt.t.tgtttt.TTTCTATTTCGCAATATTAATGAc                <  1:328005/36‑1 (MQ=255)
                         ttt.t.tttttt.tTTTTATTTCGCAATATTAATGACAg              >  1:6993050/1‑36 (MQ=25)
                         ttt.t.tgtttt.tTTCTATTTCGCAATATTAATGACAg              >  1:80790/1‑36 (MQ=255)
                          tt.t.tgtttt.tTTCTATTTCGCAATATTAATGACAgt             >  1:2075596/1‑36 (MQ=255)
                          tt.t.tgtttt.tTTCTATTTCGCAATATTAATGACAgt             <  1:1571200/36‑1 (MQ=255)
                           t.t.tgtttt.tTTCTATTTCGCAATATTAATGACAgtg            >  1:2967728/1‑36 (MQ=255)
                             t.tgtttt.tTTCTATTTCGTAATATTAATGACAgtgt           >  1:2442516/1‑36 (MQ=255)
                                 tttt.tttCTATTTCGCAATATTAATGACAGTGTcag        >  1:6811203/1‑36 (MQ=255)
                                   tt.tttCTATTTCGCAATATTAATGACAGTGTcagca      >  1:3396687/1‑36 (MQ=255)
                                   tt.tttCTAGTTCGCAATATTAATGACAGTGTcagca      <  1:3634158/36‑1 (MQ=255)
                                        tCTATGTCGAAAGATTAATGACAGTGTCAGCAGata  <  1:4911284/36‑1 (MQ=21)
                                         |                                  
AATGTTTTTTTTAGTTG.TC.TTG.TTT.T.TGTTTT.TTTCTATTTCGCAATATTAATGACAGTGTCAGCAGATA  >  REL606/4462979‑4463048

Base quality scores:  ATCG  < 3 ≤  ATCG  < 5 ≤  ATCG  < 10 ≤  ATCG  < 23 ≤  ATCG  < 32 ≤  ATCG