Read alignment evidence...
  seq id position change freq score reads annotation genes product
*REL6064,463,0060G→T43.9% 141.8143V82F (GTT→TTT) yjgNconserved inner membrane protein
Reads supporting (aligned to +/- strand):  new base (0/61):  ref base (21/57):  total (21/122)

Only 100 of 163 total aligned reads displayed.

CTGGTGGGAATGTTTTTTTTAGTT.G.TC.TT.G.TTT.TTGTTTTTTTCTATTTCGCAATATTAATGACAGTGTC  >  REL606/4462971‑4463040
                                         |                                  
cTGGTGGGAATGTTTTTTTTAGTT.G.TC.tt.g.ttt.ttg                                    <  1:7007153/36‑1 (MQ=255)
  ggtggGAATGTTTTTGTTAGTT.G.TC.tt.g.ttt.ttgtt                                  <  1:6356113/36‑1 (MQ=255)
   gtggGAATGTTTTTTTTAGTT.G.TC.tt.g.ttt.ttgttt                                 >  1:5324622/1‑36 (MQ=255)
       ttgtGTTTGTTTTGTTT.T.TT.TT.T.TTG.TTTttttttt                             <  1:6953552/33‑1 (MQ=2)
       ttctGTTTATTTTTTTT.G.TT.TT.T.GTT.TTTtttattt                             <  1:6590354/33‑1 (MQ=0)
       tattGTCTTTTTTGTTT.T.TT.TT.T.TTG.TTTttttttt                             <  1:6469574/33‑1 (MQ=2)
       gtgtGTTTTTTTTCTTT.T.TT.TT.T.GTT.TTTttttttt                             <  1:3054908/33‑1 (MQ=2)
       atgtGTGTTTTTTTTTT.T.TT.TT.G.TTT.TTTttttttt                             <  1:3668999/33‑1 (MQ=2)
        tttGTTTTTTTTTGTG.T.TT.TT.T.TTT.TTGtttttttt                            <  1:3651341/34‑2 (MQ=9)
        tttGTTTCTTTTATTT.A.T..TT.T.TTG.TTTTTTTTTTTt                           <  1:6620494/34‑1 (MQ=12)
        tgtGTTTTTATCTGTT.G.TT.TTCG.TTT.TTGttttttt                             <  1:7384049/34‑1 (MQ=2)
        tgtGTTTGTTTTGTTT.T.TG.TT.T.TTT.TTTtttttttt                            <  1:6827770/34‑2 (MQ=9)
        tcggTTTTTTTGTGTT.T.TT.TG.T.TTT.TTGTTTTTTTc                            <  1:3561262/33‑1 (MQ=2)
        gttGTTTTTGTTTTTT.T.TT.TT.G.TTT.TTTtttttttt                            <  1:6583772/34‑2 (MQ=2)
        aaTGTTTTTTTTAGTT.G.TC.TT.G.TTT.TTGtttttttt                            >  1:2248449/1‑35 (MQ=255)
        aaTGTTTTTTTTAGTT.G.TC.TT.G.TTT.TTGTTTTTTTc                            <  1:3808001/36‑1 (MQ=255)
         ttGTTTTGTTTTTTT.T.TA.TT.T.TTT.TTGttttttttt                           <  1:7264490/35‑3 (MQ=2)
         ttGTTTCTTTTGGTG.T.TT.TT.T.TTT.TTGttttttttt                           <  1:6768554/35‑3 (MQ=2)
         ttGTGTTTTTTTTTT.T.TG.TT.T.TTT.TTTTTTTTTTGt                           <  1:7115022/35‑1 (MQ=9)
         gtGTTTTCTTTTGTT.T.TT.TT.GTTTT.TTGtttttttt                            <  1:6856863/35‑2 (MQ=0)
         aTGTTTTTTTTAGTT.G.TC.TT.G.TTT.TTGTTTTTTTCt                           <  1:2552385/36‑1 (MQ=255)
         aTGTTTTTTTTAGTT.G.TC.TT.G.TTT.TTGTTTTTTTCt                           <  1:7160511/36‑1 (MQ=255)
          tGTTTTTTTATTTT.T.TT.TT.GTTTT.TTGttttttttt                           <  1:7301630/36‑3 (MQ=2)
          tGTTTTGTTTTGTT...TT.TT.G.TTT.TT.TTTTTTTCTTtt                        <  1:7373668/36‑1 (MQ=2)
          tGTTTGTTTTTGTT.T.TT.TT.G.TTT.TTTttttttttgt                          <  1:6660208/36‑4 (MQ=0)
          tGTTGTTTTTTTTT.T.TT.TT.G.TTT.TTGtttttttttt                          <  1:6486124/36‑4 (MQ=2)
          tGTCTTTTTTTTTT.T.TT.TT.T.TTT.TTGttttttttct                          <  1:6982795/36‑4 (MQ=2)
          gtttttttttGTTT.T.TG.TT.T.TTT.TTTTTTTTTTGtt                          <  1:3461035/34‑2 (MQ=2)
           tttttttttGTTT.T.TG.TT.T.TGT.TTGTTTTTTTTTTt                         <  1:4238264/35‑1 (MQ=2)
           ttttttttgTGTT.GTTG.TT.T.TTT.TTGTTTTTTTCtt                          <  1:7320185/35‑2 (MQ=2)
           tttttttctTTTT.G.TT.TT.GTTTT.TTGTTTTTTTCtt                          <  1:7030189/35‑2 (MQ=0)
           ttttttgttGGTG.G.TT.TT.T.TTT.TTTTGTTTTTTTTt                         <  1:6924454/35‑1 (MQ=0)
           gTTTTTTTTAGTT.G.TC.TT.G.TTT.TTGTTTTTTTCttt                         >  1:311559/1‑34 (MQ=255)
           gTTTTTTGTTTTT.T.TG.TT.T.TTT.TTTTTTTTTGCttt                         <  1:7320080/36‑3 (MQ=2)
           gTTCTTTTTGGTTCG.TG.TT.T.TTT.TTGtttttttttt                          <  1:7190462/36‑4 (MQ=0)
            ttttttttTTTT.T.TC.TT.T.AAT.TTGTTTTTTTCTTtt                        <  1:7301756/36‑1 (MQ=2)
            ttttttttGTTT.G.TT.TT.T.GTT.TTTTTTTTTGCtttc                        <  1:7347477/36‑4 (MQ=2)
            ttctttttGTTT.T.TC.TT.T.TTT.TTTTGTTTTTTTTtt                        <  1:7200181/33‑1 (MQ=0)
            tgttttttA.TT.T.TC.TT.G..TC.TTGTTTTTCTTTCTTTc                      <  1:6856852/34‑1 (MQ=9)
            tgttttttA.TT.C.TC.TT.T.TTG.TTTTTTTTTTTTTttt                       <  1:7011272/34‑1 (MQ=9)
            gtatttttATTT.T.TT.TT.T.TGT.TTTTTTTTTTTTTtt                        <  1:7218219/33‑1 (MQ=9)
            ctttttttTTTT.G.T..TT.A.TTT.TTGTTTTTTTTTTttt                       <  1:6477284/35‑1 (MQ=2)
             tttttttTCTT.G.TT.TT.T.GTT.TTTTCTTTTTTTTttt                       <  1:6510252/36‑1 (MQ=9)
             tttttttGTTT.T.TG.TT.T.TTT.TTGTTTTTTTGTTttt                       <  1:6580363/36‑1 (MQ=2)
             tttttttAGTT.G.TC.TT.G.TTT.TTGTTTTTTTTTAttt                       >  1:7135059/1‑36 (MQ=255)
             tttttttAGTT.G.TC.TT.G.TTT.TTGTTTTTTTCTAttt                       >  1:4579394/1‑36 (MQ=255)
             ttgttttTGTT.C.TT.TT.T.TTT.TTGTTTTTTTTTTttt                       <  1:3368036/33‑1 (MQ=2)
             tgtttttTGTT.G.TT.TT.T.TTT.TTGTTTTTTTTTTttt                       <  1:3570334/34‑1 (MQ=2)
             gttttttTTTT.T.TG.TT.T.TTT.TCTTTTGTTTCTGttt                       <  1:6500252/35‑1 (MQ=2)
             gttttttTTTT.G.TT.GT.T.TTT.TTGTTTTTTTTTTttt                       <  1:6458944/35‑1 (MQ=2)
             gttttttGGTT.T.TT.TT.T.TTT.TTGTTTTTTTTTTttt                       <  1:7328214/35‑1 (MQ=0)
             gttttttATTT.T.TT.TT.G.TTT.TTTTATTTTTTTTttt                       <  1:7473958/35‑1 (MQ=0)
             gttttctAGTT.G....TT.G.TTT.TTGTTTTTTTTTTttttt                     <  1:6924536/35‑3 (MQ=2)
             cttttttTTTT.T.TT.TT.G.TTT.TTTTTTTGTTCTTttt                       <  1:7115177/35‑1 (MQ=2)
              ttttttTTTT.G.TT.TT.T.TTG.TTTTTTTTCTTTTTTTc                      <  1:7237196/36‑1 (MQ=2)
              ttttttTTTT.C.TT.TT.G.TTT.TTTTGTTTTTTTTtgtt                      <  1:6982431/36‑4 (MQ=0)
              ttttttTTTT.C.TG.TT.C.TTT.TTGTTTTTTTTTCtttt                      <  1:6895488/36‑2 (MQ=0)
              ttttttTGTT.T.TT.TT.T.TTG.TTTTTTTTTTTTGtttg                      <  1:4675564/36‑2 (MQ=2)
              ttttttTCTT.G.TC.TT...TTT.TTGTTTTTTTTTTttttt                     <  1:6798474/36‑3 (MQ=0)
              tttttcTGTT.T.TT.TT.G.TTT.TTTTTTCTTTTTAttgt                      <  1:6768905/36‑3 (MQ=2)
              ttttatATTT.T.TT.TT.G.TTT.TTTGTTTTTTTTAtttt                      <  1:6933943/36‑2 (MQ=2)
              gtttttCGTT.T.TT.TT.T.TTT.TTGTTTTTTTTTTtttt                      <  1:6520416/35‑2 (MQ=2)
              gattttTGTT.T.TT.TT.G.TTT.TTTTTTTTTTTTTtttt                      <  1:7456279/34‑2 (MQ=9)
               tttttTGTT.G.TT.TG.T.TTT.TTGTGTTTTTGTTttttt                     <  1:6699914/36‑3 (MQ=2)
               tttttTCTT.G.TC.TT.T.TTG.TTTTTTTTTTTTTttttt                     <  1:6680134/36‑3 (MQ=0)
               tttttCTTT.G.TCTTT.G.TTT.TTTTTTTGTTTTTtttt                      <  1:6529126/36‑2 (MQ=0)
               tttttATTT.G.TT.GT.T.TTT.TTGTTTTTTTTTTttttt                     <  1:7039604/36‑3 (MQ=2)
               tttttAGTT.G.TC.TT.G.TTT.TTGTTTTTTTCTTTTTct                     >  1:4688369/1‑35 (MQ=255)
               tttttAGTT.G.TC.TT.G.TTT.TTGTTTTTTTCTATTTct                     >  1:7049367/1‑35 (MQ=255)
               tttttAGTT.G.TC.TT.G.TTT.TTGTTTTTTTCTATTTct                     >  1:2475464/1‑35 (MQ=255)
               gttttAATT.T.TT.TT.T.TTG.TTTTTTTTTTTTTTTTct                     <  1:6837370/35‑2 (MQ=9)
                ttttTGTT.G.GT.TT.T.TTG.TTTTTTTTTTTTTtttttt                    <  1:7365647/36‑4 (MQ=2)
                ttttTGTT.C.TT.TT.T.TTT.TTTTGTTTTTTTTTTTctt                    <  1:7255717/36‑3 (MQ=2)
                ttttAGTT.G.TC.TT.G.TTT.TTGTTTTTTTCTATTTCGc                    <  1:257317/36‑1 (MQ=255)
                 tttCGTT.G.TT.TT.T.TTTGTTTTTTTTTTGTTtttttt                    <  1:6953814/36‑4 (MQ=2)
                 tttAGTT.G.TC.TT.G.TTT.TTGTTTTTTTCTATTTCGCa                   >  1:6137428/1‑36 (MQ=255)
                  ttAGTT.G.TC.TT.G.TTT.TTGTTTTTTTCTATTTCTCaa                  >  1:7385047/1‑36 (MQ=255)
                   tAGTT.G.TC.TT.G.TTT.TTGTTTTTTTCTATTTCGCAat                 <  1:4099861/36‑1 (MQ=255)
                   tAGTT.G.TC.TT.G.TTT.TTGTTTTTTTCTATTTCGCAat                 >  1:4872804/1‑36 (MQ=255)
                    aGTT.G.TC.TT.G.TTT.TTGTTTTTTTCTATTTCTCAata                >  1:7178448/1‑36 (MQ=255)
                    aGTT.G.TC.TT.G.TTT.TTGTTTTTTTCTATTTCGCAata                <  1:3901407/36‑1 (MQ=255)
                     gTT.G.TC.TT.G.TTT.TTGTTTTTTTCTATTTCGCAatat               <  1:2821673/36‑1 (MQ=255)
                     gTT.G.TC.TT.G.TTT.TTGTTTTTTTCTATTTCGCAatat               <  1:4540145/36‑1 (MQ=255)
                      tt.G.TC.TT.G.TTT.TTGTTTGTTTCTATTTCGCAATAtt              <  1:6422616/36‑1 (MQ=255)
                       t.G.TC.TT.G.TTT.TTGTTTTTTTCTATTTCGCAATATTa             <  1:610406/36‑1 (MQ=255)
                       t.G.TC.TT.G.TTT.TTGTTTTTTTCTATTTCGCAATATTa             >  1:347989/1‑36 (MQ=255)
                       t.G.TC.TT.G.TTT.TTGTTTTTTTCTATTTCGCAATATTa             <  1:154748/36‑1 (MQ=255)
                         g.TC.TT.G.TTT.TTGTTTTTTTCTATTTCGCAATATTaa            >  1:4409435/1‑36 (MQ=255)
                           tC.TT.G.TTT.TTGTTTTTTTCTATTTCGCAATATTAAt           >  1:3794360/1‑36 (MQ=255)
                           tC.TT.G.TTT.TTGTTTTTTTCTATTTCGCAATATTAAt           <  1:956878/36‑1 (MQ=255)
                            c.TT.G.TTT.TTGTTTTTTTCTATTTCGCAATATTAATg          <  1:5295601/36‑1 (MQ=255)
                            c.TT.G.TTT.TTGTTTTTTTCTATTTCGCAATATTAATg          >  1:3386959/1‑36 (MQ=255)
                              tt.g.ttt.ttgtttTTTTCTATTTCGCAATATTAATGa         <  1:3779418/36‑1 (MQ=255)
                                   ttt.ttttttttTTTTATTTCGCAATATTAATGACAg      >  1:6993050/1‑36 (MQ=25)
                                   ttt.ttgtttttTTCTATTTCGCAATATTAATGACAg      >  1:80790/1‑36 (MQ=255)
                                    tt.ttgtttttTTCTATTTCGCAATATTAATGACAgt     >  1:2075596/1‑36 (MQ=255)
                                    tt.ttgtttttTTCTATTTCGCAATATTAATGACAgt     <  1:1571200/36‑1 (MQ=255)
                                     t.ttgtttttTTCTATTTCGCAATATTAATGACTgtg    >  1:5479975/1‑36 (MQ=255)
                                     t.ttgtttttTTCTATTTCGCAATATTAATGACAgtg    >  1:2967728/1‑36 (MQ=255)
                                        tgtttttTTCTATTTCGCAATATTAATGACAGTGTc  <  1:2933530/36‑1 (MQ=255)
                                         |                                  
CTGGTGGGAATGTTTTTTTTAGTT.G.TC.TT.G.TTT.TTGTTTTTTTCTATTTCGCAATATTAATGACAGTGTC  >  REL606/4462971‑4463040

Base quality scores:  ATCG  < 3 ≤  ATCG  < 7 ≤  ATCG  < 10 ≤  ATCG  < 25 ≤  ATCG  < 32 ≤  ATCG