Read alignment evidence...
  seq id position change freq score reads annotation genes product
*REL6064,463,0000G→T46.3% 142.9143V80F (GTT→TTT) yjgNconserved inner membrane protein
Reads supporting (aligned to +/- strand):  new base (0/63):  ref base (21/52):  total (21/122)

Only 100 of 164 total aligned reads displayed.

GAATTACTGGTGGGAATGTTTTTTTTAGTT.G.TC.TT.G.TTTT.TGTTTT.TTTCTATTTCGCAATATTAATGAC  >  REL606/4462965‑4463034
                                       |                                     
 aaTTACTGGTGGGAATGTTTTTTTTAGTT.G.TC.tt.g.t                                     <  1:2088294/36‑1 (MQ=255)
   ttACTGGTGGGAATGTTTTTTTTAGTT.G.TC.tt.g.ttt                                   <  1:6881064/36‑1 (MQ=255)
     aCTGGTGGGAATGTTTTTTTTAGTT.G.TC.tt.g.tttt.t                                >  1:3229533/1‑36 (MQ=255)
     aCTGGTGGGAATGTTTTTTTTAGTT.G.TC.tc.t.tttt.t                                >  1:1484458/1‑36 (MQ=25)
      cTGGTGGGAATGTTTTTTTTAGTT.G.TC.tt.g.tttt.tg                               <  1:7007153/36‑1 (MQ=255)
        ggtggGAATGTTTTTTTTAGTT.G.TC.tt.g.tttt.tgtt                             >  1:4706929/1‑36 (MQ=255)
        ggtggGAATGTTTTTGTTAGTT.G.TC.tt.g.tttt.tgtt                             <  1:6356113/36‑1 (MQ=255)
         gtggGAATGTTTTTTTTAGTT.G.TC.tt.g.tttt.tgttt                            >  1:5324622/1‑36 (MQ=255)
          tggGAATGTTTTTTTTAGTT.G.TC.tt.g.tttt.tgttta                           >  1:533572/1‑35 (MQ=255)
          tggGAATGTTTTTTTTAGTT.G.TC.tt.g.tttt.tgttct                           <  1:5667515/36‑3 (MQ=255)
             ttgtGTTTGTTTTGTTT.T.TT.TT.T.TTGT.TTtttt.ttt                       <  1:6953552/33‑1 (MQ=2)
             tattGTCTTTTTTGTTT.T.TT.TT.T.TTGT.TTtttt.ttt                       <  1:6469574/33‑1 (MQ=2)
             gtgtGTTTTTTTTCTTT.T.TT.TT.T.GTTT.TTtttt.ttt                       <  1:3054908/33‑1 (MQ=2)
              tttGTTTTTTTTTGTG.T.TT.TT.T.TTTT.TGtttt.tttt                      <  1:3651341/34‑2 (MQ=9)
              tttGTTTCTTTTATTT.A.T..TT.T.TTGT.TTTTTT.TTTTt                     <  1:6620494/34‑1 (MQ=12)
              tttGTCTTTTTTTTTT.T.TG.TT.T.TTTT.TTTTTT.TTTc                      <  1:6866358/34‑1 (MQ=9)
              tgtGTTTTTATCTGTT.G.TT.TTCG.TTTT.TGtttt.ttt                       <  1:7384049/34‑1 (MQ=2)
              tcggTTTTTTTGTGTT.T.TT.TG.T.TTTT.TGTTTT.TTTc                      <  1:3561262/33‑1 (MQ=2)
              gttGTTTTTGTTTTTT.T.TT.TT.G.TTTT.TTtttt.tttt                      <  1:6583772/34‑2 (MQ=2)
              cgtGTGTTTTGTTGTT.T.TT.TT.T.TTTT.TGtttt.tttt                      <  1:7044197/34‑2 (MQ=0)
               ttGTTTTTTTTTTTT.T.GC.TT.C.TTTT.TGtttt.tttta                     <  1:7115233/35‑3 (MQ=2)
               ttGTTTCTTTTGGTG.T.TT.TT.T.TTTT.TGtttt.ttttt                     <  1:6768554/35‑3 (MQ=2)
               ttGTGTTTTTTTTTT.T.TG.TT.T.TTTT.TTTTTT.TTTGt                     <  1:7115022/35‑1 (MQ=9)
               ttGTGTTTTTTGTTT.T.TT.TT.T.TTTT.TGtttt.ttttt                     <  1:7264220/35‑3 (MQ=2)
               gtGTTTTGTTTATTT.G.TT.TTCT.TTTT.TGtttt.tttt                      <  1:7365761/35‑2 (MQ=2)
               aTGTTTTTTTTAGTT.G.TC.TT.G.TTTT.TGtttt.ttttt                     >  1:373022/1‑34 (MQ=255)
               aTGTTTTTTTTAGTT.G.TC.TT.G.TTTT.TGTTTT.TTTCt                     <  1:7160511/36‑1 (MQ=255)
               aTGTTTTTTTTAGTT.G.TC.TT.G.TTTT.TGTTTT.TTTCt                     >  1:1883715/1‑36 (MQ=255)
               aTGTTTTTTTTAGTT.G.TC.TT.G.TTTT.TGTTTT.TTTCt                     <  1:2552385/36‑1 (MQ=255)
                ttgttttttgTGTT.G.T..TT.G.TTTT.TTTGTT.TTTTTTt                   <  1:4239137/33‑1 (MQ=0)
                tcttttttttGTTT.T.TT.TT.G.TTTT.TTTTTTATTTCt                     <  1:7237157/34‑1 (MQ=0)
                tGTTTTTTTTTTTT.T.TA.TT.G.TTTT.TTtgtt.tttttt                    <  1:6953773/36‑4 (MQ=0)
                tGTTTTTTTTGTTT.T.TT.TT.G.TTTT.TTTTTT.TTTCtt                    <  1:7300424/36‑2 (MQ=9)
                tGTTTTTTTATTTT.T.TT.TT.GTTTTT.TGtttt.ttttt                     <  1:7301630/36‑3 (MQ=2)
                tGTTTGTTTTTGTT.T.TT.TT.G.TTTT.TTtttt.ttttgt                    <  1:6660208/36‑4 (MQ=0)
                tGTTGTTTTTTTTT.T.TT.TT.G.TTTT.TGtttt.tttttt                    <  1:6486124/36‑4 (MQ=2)
                tGTTCTTTTCTGTT.G.TG.CT.G.TTCT.TTttgt.tttttt                    <  1:6827796/36‑4 (MQ=0)
                tGTCTTTTTTTTTT.T.TT.TT.T.TTTT.TGtttt.ttttct                    <  1:6982795/36‑4 (MQ=2)
                gtttttttttGTTT.T.TG.TT.T.TTTT.TTTTTT.TTTGtt                    <  1:3461035/34‑2 (MQ=2)
                 tttttttttGTTT.T.TG.TT.T.TTTTCTCTTTT.TTTCtt                    <  1:3026018/35‑2 (MQ=2)
                 tttttttttAGTT.G.TC.TT.G.TTTT.TGTTTT.TTTCttt                   >  1:7467831/2‑34 (MQ=255)
                 ttttttttgTGTT.GTTG.TT.T.TTTT.TGTTTT.TTTCtt                    <  1:7320185/35‑2 (MQ=2)
                 tttttttctTTTT.G.TT.TT.GTTTTT.TGTTTT.TTTCtt                    <  1:7030189/35‑2 (MQ=0)
                 ttttttgttGGTG.G.TT.TT.T.TTTT.TTTGTT.TTTTTTt                   <  1:6924454/35‑1 (MQ=0)
                 gTTTTTTTTAGTT.G.TC.TT.G.TTTT.TGTTTT.TTTCTAt                   <  1:6237629/36‑1 (MQ=255)
                 gTTTTTTGTTTTT.T.TG.TT.T.TTTT.TTTTTT.TTGCttt                   <  1:7320080/36‑3 (MQ=2)
                 gTTCTTTTTGGTTCG.TG.TT.T.TTTT.TGtttt.tttttt                    <  1:7190462/36‑4 (MQ=0)
                  ttttttttTGTT.C.TT.TT.A.TTTT.TTTGTT.TTTTTTtt                  <  1:7310586/36‑1 (MQ=2)
                  ttttttttGTTT.G.TT.TT.T.GTTT.TTTTTT.TTGCtttc                  <  1:7347477/36‑4 (MQ=2)
                  ttttgtttTGTT...TC.TT.G.TTTT.TTTTTT.GTTTTTttt                 <  1:7011359/36‑1 (MQ=2)
                  tttattttTGTT.T.TT.TT.T.TGTT.TTTTTT.TTTGTTtt                  <  1:6580355/36‑1 (MQ=9)
                  ttctttttGTTT.T.TC.TT.T.TTTT.TTTGTT.TTTTTTtt                  <  1:7200181/33‑1 (MQ=0)
                  tgttttttA.TT.T.TC.TT.G..TCT.TGTTTT.TCTTTCTTTc                <  1:6856852/34‑1 (MQ=9)
                  gcttttttTGTT...TT.TT.G.TTTT.TTTTTT.TTTTTTttt                 <  1:7067339/34‑1 (MQ=0)
                  ctttttttTTTT.G.T..TT.A.TTTT.TGTTTT.TTTTTTttt                 <  1:6477284/35‑1 (MQ=2)
                   tttttttTTTT.G..C.TT.T.TTTT.TTTTTT.TTTCTAtttt                <  1:6973006/36‑2 (MQ=9)
                   tttttttTCTT.G.TT.TT.T.GTTT.TTTCTT.TTTTTTttt                 <  1:6510252/36‑1 (MQ=9)
                   tttttttGTTT.T.TG.TT.T.TTTT.TGTTTT.TTTGTTttt                 <  1:6580363/36‑1 (MQ=2)
                   tttttttAGTT.G.TC.TT.G.TTTT.TGTTTT.TTTTTAttt                 >  1:7135059/1‑36 (MQ=255)
                   tttttttAGTT.G.TC.TT.G.TTTT.TGTTTT.TTTCTAttt                 >  1:4579394/1‑36 (MQ=255)
                   tttttttA.TT.T.TG.TT.T.TTTT.CGTTTC.TTTTTTtttt                <  1:6992331/36‑2 (MQ=0)
                   ttttttgCGTT.G.TT.TT.T.TGTT.TTTTTT.TTTTTTttt                 <  1:7393115/36‑1 (MQ=2)
                   ttttgttTGTT.G.TT.TT.T.TGTT.TTTTTT.TTTTTTttt                 <  1:3862874/36‑1 (MQ=0)
                   ttgttttTGTT.C.TT.TT.T.TTTT.TGTTTT.TTTTTTttt                 <  1:3368036/33‑1 (MQ=2)
                   tgtttttTGTT.G.TT.TT.T.TTTT.TGTTTT.TTTTTTttt                 <  1:3570334/34‑1 (MQ=2)
                   gttttttTTTT.T.TG.TT.T.TTTT.CTTTTG.TTTCTGttt                 <  1:6500252/35‑1 (MQ=2)
                   gttttttTTTT.G.TT.GT.T.TTTT.TGTTTT.TTTTTTttt                 <  1:6458944/35‑1 (MQ=2)
                   gttttttGTTT.T.TT.TT.G.TTTT.TTTTTT.ATTTTTttt                 <  1:6905235/35‑1 (MQ=2)
                   gttttttATTT.T.TT.TT.G.TTTT.TTTATT.TTTTTTttt                 <  1:7473958/35‑1 (MQ=0)
                   cttttttTGTT.T.TT.TT.T.TTTT.TGTTTT.TTT.TAtttt                <  1:7329393/35‑2 (MQ=2)
                    ttttttTTTT.G.TT.TT.T.TTGT.TTTTTT.TCTTTTTTTc                <  1:7237196/36‑1 (MQ=2)
                    ttttttTGTT.T.TG.TG.T.TTTT.TGTTTT.TTTTTTtttt                <  1:7181282/36‑2 (MQ=2)
                    ttttttTCTT.G.TC.TT...TTTT.TGTTTT.TTTTTTttttt               <  1:6798474/36‑3 (MQ=0)
                    tttttcTGTT.T.TT.TT.G.TTTT.TTTTTC.TTTTTAttgt                <  1:6768905/36‑3 (MQ=2)
                    gtttttTTTT.G.TT.TT.A.TTTT.CTTTTT.TGTTTTtttg                <  1:6924677/35‑2 (MQ=2)
                    gtttttCGTT.T.TT.TT.T.TTTT.TGTTTT.TTTTTTtttt                <  1:6520416/35‑2 (MQ=2)
                     tttttTGTT.T.TC.TT...TTTT.GTTTTT.TTTTTAtttttt              <  1:7374361/36‑4 (MQ=0)
                     tttttTGTT.G.TT.TG.T.TTTT.TGTGTT.TTTGTTttttt               <  1:6699914/36‑3 (MQ=2)
                     tttttTCTT.G.TC.TT.T.TTGT.TTTTTT.TTTTTTttttt               <  1:6680134/36‑3 (MQ=0)
                     tttttAGTT.G.TC.TT.G.TTTT.TGTTTT.TTTCTTTTTct               >  1:4688369/1‑35 (MQ=255)
                     tttttAGTT.G.TC.TT.G.TTTT.TGTTTT.TTTCTATTTct               >  1:2475464/1‑35 (MQ=255)
                     gttttAATT.T.TT.TT.T.TTGT.TTTTTT.TTTTTTTTTct               <  1:6837370/35‑2 (MQ=9)
                      ttttTGTT.G.TTTTT.G.TTTT.TTTTTT.TTTATTttttt               <  1:6600332/36‑3 (MQ=2)
                      ttttAGTT.G.TC.TT.G.TTTT.TGTTTT.TTTCTATTTCGc              <  1:257317/36‑1 (MQ=255)
                       tttAGTT.G.TC.TT.G.TTTT.TGTTTT.TTTCTATTTCGCa             >  1:6137428/1‑36 (MQ=255)
                       tttAGTT.G.TC.TT.G.TTTT.TGTTTT.TTTCTATTTCGCa             <  1:5024875/36‑1 (MQ=255)
                         tAGTT.G.TC.TT.G.TTTT.TGTTTT.TTTCTATTTCGCAat           <  1:4099861/36‑1 (MQ=255)
                          aGTT.G.TC.TT.G.TTTT.TGTTTT.TTTCTATTTCGCAata          <  1:3901407/36‑1 (MQ=255)
                           gTT.G.TC.TT.G.TTTT.TGTTTT.TTTCTATTTCGCAatat         <  1:4540145/36‑1 (MQ=255)
                           gTT.G.TC.TT.G.TTTT.TGTTTT.TTTCTATTTCGCAatat         <  1:2821673/36‑1 (MQ=255)
                            tt.G.TC.TT.G.TTTT.TGTTTG.TTTCTATTTCGCAATAtt        <  1:6422616/36‑1 (MQ=255)
                             t.G.TC.TT.G.TTTT.TGTTTT.TTTCTATTTCGCAATATTa       <  1:154748/36‑1 (MQ=255)
                             t.G.TC.TT.G.TTTT.TGTTTT.TTTCTATTTCGCAATATTa       >  1:347989/1‑36 (MQ=255)
                               g.TC.TT.G.TTTT.TGTTTT.TTTCTATTTCGCAATATTaa      >  1:4409435/1‑36 (MQ=255)
                                 tC.TT.G.TTTT.TGTTTT.TTTCTATTTCGCAATATTAAt     <  1:956878/36‑1 (MQ=255)
                                 tC.TT.G.TTTT.TGTTTT.TTTCTATTTCGCAATATTAAt     >  1:3794360/1‑36 (MQ=255)
                                  c.TT.G.TTTT.TGTTTT.TTTCTATTTCGCAATATTAATg    >  1:3386959/1‑36 (MQ=255)
                                  c.TT.G.TTTT.TGTTTT.TTTCTATTTCGCAATATTAATg    >  1:4665578/1‑36 (MQ=255)
                                    tt.g.tttt.tgtttT.TTTCTATTTCGCAATATTAATGa   <  1:3779418/36‑1 (MQ=255)
                                     t.g.tttt.tgtttt.TTTCTATTTCGCAATATTAATGAc  <  1:328005/36‑1 (MQ=255)
                                       |                                     
GAATTACTGGTGGGAATGTTTTTTTTAGTT.G.TC.TT.G.TTTT.TGTTTT.TTTCTATTTCGCAATATTAATGAC  >  REL606/4462965‑4463034

Base quality scores:  ATCG  < 3 ≤  ATCG  < 5 ≤  ATCG  < 10 ≤  ATCG  < 23 ≤  ATCG  < 32 ≤  ATCG