Read alignment evidence...
  seq id position change freq score reads annotation genes product
*REL6064,462,9970C→T51.1% 146.7159L79F (CTT→TTT) yjgNconserved inner membrane protein
Reads supporting (aligned to +/- strand):  new base (1/69):  ref base (28/39):  total (29/130)

Only 100 of 165 total aligned reads displayed.

ATGGAATTACTGGTGGGAATGTTTTTTTTAGTT.G.TC.TT.G.TTTTTGTTTTTTTCTATTTCGCAATATTAATG  >  REL606/4462962‑4463032
                                     |                                      
aTGGAATTACTGGTGGGAATGTTTTTTTTAGTT.G.Tc                                        >  1:6229027/1‑36 (MQ=255)
aTGGAATTACTGGTGGGAATGTTTTTTTTAGTT.G.Tc                                        >  1:5465227/1‑36 (MQ=255)
 tGGAATTACTGGTGGGAATGTTTTTTTTAGTT.G.TC.t                                      <  1:6428469/36‑1 (MQ=255)
   gAATTACTGGTGGGAATGTTTTTTTTAGTT.G.TC.tt.n                                   <  1:6349846/36‑1 (MQ=255)
    aaTTACTGGTGGGAATGTTTTTTTTAGTT.G.TC.tt.g.t                                 <  1:2088294/36‑1 (MQ=255)
    aaTTACTGGTGGGAATGTTTTTTTTAGTT.G.TC.tt.g.t                                 <  1:3261198/36‑1 (MQ=255)
      ttACTGGTGGGAATGTTTTTTTTAGTT.G.TC.tt.g.ttt                               <  1:6881064/36‑1 (MQ=255)
        aCTGGTGGGAATGTTTTTTTTAGTT.G.TC.tc.t.ttttt                             >  1:1484458/1‑36 (MQ=25)
         cTGGTGGGAATGTTTTTTTTAGTT.G.TC.tt.g.tttttg                            <  1:7007153/36‑1 (MQ=255)
           ggtggGAATGTTTTTTTTAGTT.G.TC.tt.g.tttttgtt                          >  1:4706929/1‑36 (MQ=255)
           ggtggGAATGTTTTTGTTAGTT.G.TC.tt.g.tttttgtt                          <  1:6356113/36‑1 (MQ=255)
            gtggGAATGTTTTTTTTAGTT.G.TC.tt.g.tttttgttt                         >  1:5324622/1‑36 (MQ=255)
             tggGAATGTTTTTTTTAGTT.G.TC.tt.g.tttttgttta                        >  1:533572/1‑35 (MQ=255)
             tggGAATGTTTTTTTTAGTT.G.TC.tt.g.tttttgttct                        <  1:5667515/36‑3 (MQ=255)
                ttctGTTTATTTTTTTT.G.TT.TT.T.GTTTTTtttattt                     <  1:6590354/33‑1 (MQ=0)
                gtgtGTTTTTTTTCTTT.T.TT.TT.T.GTTTTTttttttt                     <  1:3054908/33‑1 (MQ=2)
                atgtGTGTTTTTTTTTT.T.TT.TT.G.TTTTTTttttttt                     <  1:3668999/33‑1 (MQ=2)
                 tttGTTTCTTTTATTT.A.T..TT.T.TTGTTTTTTTTTTTt                   <  1:6620494/34‑1 (MQ=12)
                 tgtGTTTTTATCTGTT.G.TT.TTCG.TTTTTGttttttt                     <  1:7384049/34‑1 (MQ=2)
                 tgtGTTTGTTTTGTTT.T.TG.TT.T.TTTTTTtttttttt                    <  1:6827770/34‑2 (MQ=9)
                 tcggTTTTTTTGTGTT.T.TT.TG.T.TTTTTGTTTTTTTc                    <  1:3561262/33‑1 (MQ=2)
                 cgtGTGTTTTGTTGTT.T.TT.TT.T.TTTTTGtttttttt                    <  1:7044197/34‑2 (MQ=0)
                 cgtGTCTTTTTTTGTT.T.TT.TT.G.TTTTTTtttttttt                    <  1:6510358/34‑2 (MQ=0)
                  ttGTTTTTTTTTTTT.T.GC.TT.C.TTTTTGtttttttta                   <  1:7115233/35‑3 (MQ=2)
                  ttGTTTTGTTTTTTT.T.TA.TT.T.TTTTTGttttttttt                   <  1:7264490/35‑3 (MQ=2)
                  ttGTGTTTTTTTTTT.T.TG.TT.T.TTTTTTTTTTTTTGt                   <  1:7115022/35‑1 (MQ=9)
                  ttGTGTTTTTTGTTT.T.TT.TT.T.TTTTTGttttttttt                   <  1:7264220/35‑3 (MQ=2)
                  gtGTTTTGTTTATTT.G.TT.TTCT.TTTTTGtttttttt                    <  1:7365761/35‑2 (MQ=2)
                  gtGTTTTCTTTTGTT.T.TT.TT.GTTTTTTGtttttttt                    <  1:6856863/35‑2 (MQ=0)
                  aTGTTTTTTTTAGTT.G.TC.TT.G.TTTTTGttttttttt                   >  1:373022/1‑34 (MQ=255)
                  aTGTTTTTTTTAGTT.G.TC.TT.G.TTTTTGTTTTTTTCt                   >  1:1883715/1‑36 (MQ=255)
                  aTGTTTTTTTTAGTT.G.TC.TT.G.TTTTTGTTTTTTTCt                   <  1:2552385/36‑1 (MQ=255)
                  aTGTTTTTTTTAGTT.G.TC.TT.G.TTTTTGTTTTTTTCt                   <  1:7160511/36‑1 (MQ=255)
                   tGTTTTTTTTTTTT.T.TA.TT.G.TTTTTTtgtttttttt                  <  1:6953773/36‑4 (MQ=0)
                   tGTTTTTTTATTTT.T.TT.TT.GTTTTTTGttttttttt                   <  1:7301630/36‑3 (MQ=2)
                    tttttttttGTTT.T.TG.TT.T.TGTTTGTTTTTTTTTTt                 <  1:4238264/35‑1 (MQ=2)
                    tttttttttAGTT.G.TC.TT.G.TTTTTGTTTTTTTCttt                 >  1:7467831/2‑34 (MQ=255)
                    ttttttttgTGTT.GTTG.TT.T.TTTTTGTTTTTTTCtt                  <  1:7320185/35‑2 (MQ=2)
                    tttttttctTTTT.G.TT.TT.GTTTTTTGTTTTTTTCtt                  <  1:7030189/35‑2 (MQ=0)
                    ttttttgttGGTG.G.TT.TT.T.TTTTTTTGTTTTTTTTt                 <  1:6924454/35‑1 (MQ=0)
                    gTTTTTTTTAGTT.G.TC.TT.G.TTTTTGTTTTTTTCttt                 >  1:311559/1‑34 (MQ=255)
                    gTTTTTTGTTTTT.T.TG.TT.T.TTTTTTTTTTTTGCttt                 <  1:7320080/36‑3 (MQ=2)
                    gTTCTTTTTGGTTCG.TG.TT.T.TTTTTGtttttttttt                  <  1:7190462/36‑4 (MQ=0)
                     ttttttttTGTT.G..C.TT.T.TTGTTTTTTTTTCCTGttt               <  1:3497517/36‑1 (MQ=0)
                     ttttttttTGTT.C.TT.TT.A.TTTTTTTGTTTTTTTTtt                <  1:7310586/36‑1 (MQ=2)
                     ttttttttGTTT.G.TT.TT.T.GTTTTTTTTTTTGCtttc                <  1:7347477/36‑4 (MQ=2)
                     ttctttttGTTT.T.TC.TT.T.TTTTTTTGTTTTTTTTtt                <  1:7200181/33‑1 (MQ=0)
                     tgttttttA.TT.T.TC.TT.G..TCTTGTTTTTCTTTCTTTc              <  1:6856852/34‑1 (MQ=9)
                     tgttttttA.TT.C.TC.TT.T.TTGTTTTTTTTTTTTTttt               <  1:7011272/34‑1 (MQ=9)
                     gtatttttATTT.T.TT.TT.T.TGTTTTTTTTTTTTTTtt                <  1:7218219/33‑1 (MQ=9)
                     ctttttttTTTT.G.T..TT.A.TTTTTGTTTTTTTTTTttt               <  1:6477284/35‑1 (MQ=2)
                      tttttttTTTT.G..C.TT.T.TTTTTTTTTTTTTCTAtttt              <  1:6973006/36‑2 (MQ=9)
                      tttttttTGTT...TT.TT.G.TTTTT.TTTTTTTGTAttttt             <  1:6480639/36‑3 (MQ=0)
                      tttttttTCTT.G.TT.TT.T.GTTTTTTCTTTTTTTTttt               <  1:6510252/36‑1 (MQ=9)
                      tttttttGTTT.T.TG.TT.T.TTTTTGTTTTTTTGTTttt               <  1:6580363/36‑1 (MQ=2)
                      tttttttGTTT.T.TG.TT.G.TTTTTTTTTTGTTTTTttt               <  1:6490092/36‑1 (MQ=2)
                      tttttttGTTT.A.TT.TT.G.TTTTTGTTTTTTTTTTttt               <  1:6469532/36‑1 (MQ=9)
                      tttttttAGTT.G.TC.TT.G.TTTTTGTTTTTTTTTAttt               >  1:7135059/1‑36 (MQ=255)
                      tttttttAGTT.G.TC.TT.G.TTTTTGTTTTTTTCTAttt               >  1:4579394/1‑36 (MQ=255)
                      tttttttA.TT.T.TG.TT.T.TTTTCGTTTCTTTTTTtttt              <  1:6992331/36‑2 (MQ=0)
                      ttttttgCGTT.G.TT.TT.T.TGTTTTTTTTTTTTTTttt               <  1:7393115/36‑1 (MQ=2)
                      ttttgttTGTT.G.TT.TT.T.TGTTTTTTTTTTTTTTttt               <  1:3862874/36‑1 (MQ=0)
                      tttatttGGTT.T.TT.TT.G.TTTTTTTTTTTTTTTTttt               <  1:3479249/36‑1 (MQ=9)
                      gttttttTTTT.G.TT.GT.T.TTTTTGTTTTTTTTTTttt               <  1:6458944/35‑1 (MQ=2)
                      gttttttATTT.T.TT.TT.G.TTTTTTTATTTTTTTTttt               <  1:7473958/35‑1 (MQ=0)
                      cttttttTGTT.T.TT.TT.T.TTTTTGTTTTTTT.TAtttt              <  1:7329393/35‑2 (MQ=2)
                       ttttttTTTT.G.TT.TT.T.TTGTTTTTTTTCTTTTTTTc              <  1:7237196/36‑1 (MQ=2)
                       ttttttTTTT.C.TT.TT.G.TTTTTTTGTTTTTTTTtgtt              <  1:6982431/36‑4 (MQ=0)
                       ttttttTTTT.C.TG.TT.C.TTTTTGTTTTTTTTTCtttt              <  1:6895488/36‑2 (MQ=0)
                       ttttttTGTT.T.TG.TG.T.TTTTTGTTTTTTTTTTtttt              <  1:7181282/36‑2 (MQ=2)
                       ttttttTCTT.G.TC.TT...TTTTTGTTTTTTTTTTttttt             <  1:6798474/36‑3 (MQ=0)
                       ttttttTCTT.G.CT.TT.T.TTTTTTTTTGTTTTTTtttt              <  1:6759184/36‑2 (MQ=2)
                       ttttttA.TT.T.TT.TT.T.TTTTTGTTTTTTTTTTtttgg             <  1:6640510/36‑3 (MQ=9)
                       tttttcTGTT.T.TT.TT.G.TTTTTTTTTCTTTTTAttgt              <  1:6768905/36‑3 (MQ=2)
                       tttttcTGTT.G.TT.TT.G.TTTTTTTGTTTTTTTTtttt              <  1:6580383/36‑2 (MQ=9)
                       ttttatATTT.T.TT.TT.G.TTTTTTGTTTTTTTTAtttt              <  1:6933943/36‑2 (MQ=2)
                       gtttttTTTT.G.TT.TT.A.TTTTCTTTTTTGTTTTtttg              <  1:6924677/35‑2 (MQ=2)
                        tttttTGTT.T.TC.TT...TTTTGTTTTTTTTTTAtttttt            <  1:7374361/36‑4 (MQ=0)
                        tttttTGTT.G.TT.TG.T.TTTTTGTGTTTTTGTTttttt             <  1:6699914/36‑3 (MQ=2)
                        tttttTCTT.G.TC.TT.T.TTGTTTTTTTTTTTTTttttt             <  1:6680134/36‑3 (MQ=0)
                        tttttCTTT.G.TCTTT.G.TTTTTTTTTTGTTTTTtttt              <  1:6529126/36‑2 (MQ=0)
                        tttttAGTT.G.TC.TT.G.TTTTTGTTTTTTTCTTTTTct             >  1:4688369/1‑35 (MQ=255)
                        tttttAGTT.G.TC.TT.G.TTTTTGTTTTTTTCTATTTct             >  1:7049367/1‑35 (MQ=255)
                        tttttAGTT.G.TC.TT.G.TTTTTGTTTTTTTCTATTTct             >  1:2475464/1‑35 (MQ=255)
                        gttttAATT.T.TT.TT.T.TTGTTTTTTTTTTTTTTTTct             <  1:6837370/35‑2 (MQ=9)
                         ttttAGTT.G.TC.TT.G.TTTTTGTTTTTTTCTATTTCGc            <  1:257317/36‑1 (MQ=255)
                          tttAGTT.G.TC.TT.G.TTTTTGTTTTTTTCTATTTCGCa           <  1:5024875/36‑1 (MQ=255)
                            tAGTT.G.TC.TT.G.TTTTTGTTTTTTTCTATTTCGCAat         <  1:4099861/36‑1 (MQ=255)
                             aGTT.G.TC.TT.G.TTTTTGTTTTTTTCTATTTCGCAata        <  1:3901407/36‑1 (MQ=255)
                              gTT.G.TC.TT.G.TTTTTGTTTTTTTCTATTTCGCAatat       <  1:4540145/36‑1 (MQ=255)
                              gTT.G.TC.TT.G.TTTTTGTTTTTTTCTATTTCGCAatat       <  1:2821673/36‑1 (MQ=255)
                                t.G.TC.TT.G.TTTTTGTTTTTTTCTATTTCGCAATATTa     <  1:610406/36‑1 (MQ=255)
                                t.G.TC.TT.G.TTTTTGTTTTTTTCTATTTCGCAATATTa     <  1:154748/36‑1 (MQ=255)
                                  g.TC.TT.G.TTTTTGTTTTTTTCTATTTCGCAATATTaa    >  1:4409435/1‑36 (MQ=255)
                                    tC.TT.G.TTTTTGTTTTTTTCTATTTCGCAATATTAAt   >  1:3794360/1‑36 (MQ=255)
                                    tC.TT.G.TTTTTGTTTTTTTCTATTTCGCAATATTAAt   >  1:3607742/1‑36 (MQ=255)
                                    tC.TT.G.TTTTTGTTTTTTTCTATTTCGCAATATTAAt   <  1:956878/36‑1 (MQ=255)
                                     c.TT.G.TTTTTGTTTTTTTCTATTTCGCAATATTAATg  >  1:3386959/1‑36 (MQ=255)
                                     c.TT.G.TTTTTGTTTTTTTCTATTTCGCAATATTAATg  >  1:4665578/1‑36 (MQ=255)
                                     c.TT.G.TTTTTGTTTTTTTATATTTAGAAATATTTATg  >  1:3385393/1‑36 (MQ=18)
                                     |                                      
ATGGAATTACTGGTGGGAATGTTTTTTTTAGTT.G.TC.TT.G.TTTTTGTTTTTTTCTATTTCGCAATATTAATG  >  REL606/4462962‑4463032

Base quality scores:  ATCG  < 3 ≤  ATCG  < 5 ≤  ATCG  < 10 ≤  ATCG  < 22 ≤  ATCG  < 32 ≤  ATCG