Read alignment evidence...
  seq id position change freq score reads annotation genes product
*REL6064,462,9950G→T38.9% 113.0157C78F (TGT→TTT) yjgNconserved inner membrane protein
Reads supporting (aligned to +/- strand):  new base (0/56):  ref base (28/60):  total (28/129)

Only 100 of 159 total aligned reads displayed.

TTATGGAATTACTGGTGGGAATGTTTTTTTTAGTT.G.TCTT.G.TTT.T.TGTTTTTTTCTATTTCGCAATATTAA  >  REL606/4462960‑4463030
                                    |                                        
ttATGGAATTACTGGTGGGAATGTTTTTTTTAGTT.g                                          <  1:3479190/36‑1 (MQ=255)
  aTGGAATTACTGGTGGGAATGTTTTTTTTAGTT.G.Tc                                       >  1:5465227/1‑36 (MQ=255)
  aTGGAATTACTGGTGGGAATGTTTTTTTTAGTT.G.Tc                                       >  1:6229027/1‑36 (MQ=255)
      aaTTACTGGTGGGAATGTTTTTTTTAGTT.G.TCtt.g.t                                 <  1:2088294/36‑1 (MQ=255)
        ttACTGGTGGGAATGTTTTTTTTAGTT.G.TCtt.g.ttt                               <  1:5600539/36‑1 (MQ=255)
          aCTGGTGGGAATGTTTTTTTTAGTT.G.TCtt.g.ttt.t.t                           >  1:3229533/1‑36 (MQ=255)
          aCTGGTGGGAATGTTTTTTTTAGTT.G.TCtc.t.ttt.t.t                           >  1:1484458/1‑36 (MQ=25)
           cTGGTGGGAATGTTTTTTTTAGTT.G.TCtt.g.ttt.t.tg                          <  1:7007153/36‑1 (MQ=255)
            tggtggGAATGTTTTTTTTAGTT.G.TCtt.g.ttt.t.tgt                         >  1:2521315/1‑36 (MQ=255)
             ggtggGAATGTTTTTGTTAGTT.G.TCtt.g.ttt.t.tgtt                        <  1:6356113/36‑1 (MQ=255)
              gtggGAATGTTTTTTTTAGTT.G.TCtt.g.ttt.t.tgttt                       >  1:5324622/1‑36 (MQ=255)
               tggGAATGTTTTTTTTAGTT.G.TCtt.g.ttt.t.tgttta                      >  1:533572/1‑35 (MQ=255)
               tggGAATGTTTTTTTTAGTT.G.TCTt.g.ttt.t.tgtttt                      >  1:3786295/1‑36 (MQ=255)
                  ttgtGTTTGTTTTGTTT.T.TTTT.T.TTG.T.TTttttttt                   <  1:6953552/33‑1 (MQ=2)
                  ttctGTTTATTTTTTTT.G.TTTT.T.GTT.T.TTtttattt                   <  1:6590354/33‑1 (MQ=0)
                  tattGTCTTTTTTGTTT.T.TTTT.T.TTG.T.TTttttttt                   <  1:6469574/33‑1 (MQ=2)
                   tttGTTTTTTTTTGTG.T.TTTT.T.TTT.T.TGtttttttt                  <  1:3651341/34‑2 (MQ=9)
                   tttGTTTCTTTTATTT.A.T.TT.T.TTG.T.TTTTTTTTTTt                 <  1:6620494/34‑1 (MQ=12)
                   tttGTCTTTTTTTTTT.T.TGTT.T.TTT.T.TTTTTTTTTc                  <  1:6866358/34‑1 (MQ=9)
                   tgtGTTTTTATCTGTT.G.TTTTCG.TTT.T.TGttttttt                   <  1:7384049/34‑1 (MQ=2)
                   tgtGTTTGTTTTGTTT.T.TGTT.T.TTT.T.TTtttttttt                  <  1:6827770/34‑2 (MQ=9)
                   tcggTTTTTTTGTGTT.T.TTTG.T.TTT.T.TGTTTTTTTc                  <  1:3561262/33‑1 (MQ=2)
                   gttGTTTTTGTTTTTT.T.TTTT.G.TTT.T.TTtttttttt                  <  1:6583772/34‑2 (MQ=2)
                   gaTGTTTTTTTTAGTT.G.TCTT.G.TTT.T.TGTTTTTTTc                  <  1:834183/35‑1 (MQ=255)
                   cgtGTGTTTTGTTGTT.T.TTTT.T.TTT.T.TGtttttttt                  <  1:7044197/34‑2 (MQ=0)
                    ttGTTTTTTTTTTTT.T.GCTT.C.TTT.T.TGtttttttta                 <  1:7115233/35‑3 (MQ=2)
                    ttGTTTTTTTGATTT.G.TTTT.T.TTT.G.TTttttttttt                 <  1:6449906/35‑3 (MQ=2)
                    ttGTTTTGTTTTTTT.T.TATT.T.TTT.T.TGttttttttt                 <  1:7264490/35‑3 (MQ=2)
                    ttGTGTTTTTTTTTT.T.TGTT.T.TTT.T.TTTTTTTTTGt                 <  1:7115022/35‑1 (MQ=9)
                    ttGTGTTTTTTGTTT.T.TTTT.T.TTT.T.TGttttttttt                 <  1:7264220/35‑3 (MQ=2)
                    gtGTTTTCTTTTGTT.T.TTTT.GTTTT.T.TGtttttttt                  <  1:6856863/35‑2 (MQ=0)
                    aTGTTTTTTTTTTTT.G.TTGT.T.TTT.T.TGttttttttt                 <  1:7001152/36‑3 (MQ=9)
                    aTGTTTTTTTTAGTT.G.TCTT.G.TTT.T.TGttttttttt                 >  1:373022/1‑34 (MQ=255)
                    aTGTTTTTTTTAGTT.G.TCTT.G.TTT.T.TGTTTTTTTCt                 <  1:7160511/36‑1 (MQ=255)
                    aTGTTTTTTTTAGTT.G.TCTT.G.TTT.T.TGTTTTTTTCt                 >  1:1883715/1‑36 (MQ=255)
                     tGTTTTTTTATTTT.T.TTTT.GTTTT.T.TGttttttttt                 <  1:7301630/36‑3 (MQ=2)
                     tGTTTTGTTTTGTT...TTTT.G.TTT.T.T.TTTTTTTCTTtt              <  1:7373668/36‑1 (MQ=2)
                     tGTTTGTTTTTGTT.T.TTTT.G.TTT.T.TTttttttttgt                <  1:6660208/36‑4 (MQ=0)
                     tGTTGTTTTTTTTT.T.TTTT.G.TTT.T.TGtttttttttt                <  1:6486124/36‑4 (MQ=2)
                     tGTCTTTTTTTTTT.T.TTTT.T.TTT.T.TGttttttttct                <  1:6982795/36‑4 (MQ=2)
                      tttttttttGTTT.T.TGTT.T.TTT.TCTCTTTTTTTCtt                <  1:3026018/35‑2 (MQ=2)
                      tttttttttGTTT.T.TGTT.T.TGT.T.TGTTTTTTTTTTt               <  1:4238264/35‑1 (MQ=2)
                      tttttttttAGTT.G.TCTT.G.TTT.T.TGTTTTTTTCttt               >  1:7467831/2‑34 (MQ=255)
                      ttttttttgTGTT.GTTGTT.T.TTT.T.TGTTTTTTTCtt                <  1:7320185/35‑2 (MQ=2)
                      ttttttgttGGTG.G.TTTT.T.TTT.T.TTTGTTTTTTTTt               <  1:6924454/35‑1 (MQ=0)
                      gTTTTTTTTAGTT.G.TCTT.G.TTT.T.TGTTTTTTTCttt               >  1:311559/1‑34 (MQ=255)
                      gTTTTTTTTAGTT.G.TCTT.G.TTT.T.TGTTTTTTTCTAt               <  1:6237629/36‑1 (MQ=255)
                      gTTTTTTGTTTTT.T.TGTT.T.TTT.T.TTTTTTTTGCttt               <  1:7320080/36‑3 (MQ=2)
                      gTTCTTTTTGGTTCG.TGTT.T.TTT.T.TGtttttttttt                <  1:7190462/36‑4 (MQ=0)
                       ttttttttTTTT.T.TCTT.T.AAT.T.TGTTTTTTTCTTtt              <  1:7301756/36‑1 (MQ=2)
                       ttttttttTGTT.G..CTT.T.TTG.T.TTTTTTTTCCTGttt             <  1:3497517/36‑1 (MQ=0)
                       ttttttttGTTT.G.TTTT.T.GTT.T.TTTTTTTTGCtttc              <  1:7347477/36‑4 (MQ=2)
                       ttttgtttTGTT...TCTT.G.TTT.T.TTTTTTGTTTTTttt             <  1:7011359/36‑1 (MQ=2)
                       tttattttTGTT.T.TTTT.T.TGT.T.TTTTTTTTTGTTtt              <  1:6580355/36‑1 (MQ=9)
                       ttctttttGTTT.T.TCTT.T.TTT.T.TTTGTTTTTTTTtt              <  1:7200181/33‑1 (MQ=0)
                       gtatttttATTT.T.TTTT.T.TGT.T.TTTTTTTTTTTTtt              <  1:7218219/33‑1 (MQ=9)
                       gcttttttTGTT...TTTT.G.TTT.T.TTTTTTTTTTTTttt             <  1:7067339/34‑1 (MQ=0)
                        tttttttTGTT...TTTT.G.TTT.T.T.TTTTTTTGTAttttt           <  1:6480639/36‑3 (MQ=0)
                        tttttttTCTT.G.TTTT.T.GTT.T.TTTCTTTTTTTTttt             <  1:6510252/36‑1 (MQ=9)
                        tttttttGTTT.T.TGTT.T.TTT.T.TGTTTTTTTGTTttt             <  1:6580363/36‑1 (MQ=2)
                        tttttttGTTT.A.TTTT.G.TTT.T.TGTTTTTTTTTTttt             <  1:6469532/36‑1 (MQ=9)
                        tttttttAGTT.G.TCTT.G.TTT.T.TGTTTTTTTCTAttt             >  1:4579394/1‑36 (MQ=255)
                        ttttgttTGTT.G.TTTT.T.TGT.T.TTTTTTTTTTTTttt             <  1:3862874/36‑1 (MQ=0)
                        tttatttGGTT.T.TTTT.G.TTT.T.TTTTTTTTTTTTttt             <  1:3479249/36‑1 (MQ=9)
                        ttgttttTGTT.C.TTTT.T.TTT.T.TGTTTTTTTTTTttt             <  1:3368036/33‑1 (MQ=2)
                        gttttttTTTT.T.TGTT.T.TTT.T.CTTTTGTTTCTGttt             <  1:6500252/35‑1 (MQ=2)
                        gttttttGGTT.T.TTTT.T.TTT.T.TGTTTTTTTTTTttt             <  1:7328214/35‑1 (MQ=0)
                        gttttctAGTT.G...TT.G.TTT.T.TGTTTTTTTTTTttttt           <  1:6924536/35‑3 (MQ=2)
                        gtttattTGTT.T.TTTT.T.TTG.T.TTTTTTTTTTTTttt             <  1:7143998/35‑1 (MQ=0)
                         ttttttTTTT.G.TTTT.T.TTG.T.TTTTTTTCTTTTTTTc            <  1:7237196/36‑1 (MQ=2)
                         ttttttTTTT.C.TTTT.G.TTT.T.TTTGTTTTTTTTtgtt            <  1:6982431/36‑4 (MQ=0)
                         ttttttTTTT.C.TGTT.C.TTT.T.TGTTTTTTTTTCtttt            <  1:6895488/36‑2 (MQ=0)
                         ttttttTGTT.T.TTTT.T.TTG.T.TTTTTTTTTTTGtttg            <  1:4675564/36‑2 (MQ=2)
                         ttttttTGTT.T.TGTG.T.TTT.T.TGTTTTTTTTTTtttt            <  1:7181282/36‑2 (MQ=2)
                         ttttttTCTT.G.TCTT...TTT.T.TGTTTTTTTTTTttttt           <  1:6798474/36‑3 (MQ=0)
                         ttttttTCTT.G.CTTT.T.TTT.T.TTTTTGTTTTTTtttt            <  1:6759184/36‑2 (MQ=2)
                         tttttcTGTT.T.TTTT.G.TTT.T.TTTTTCTTTTTAttgt            <  1:6768905/36‑3 (MQ=2)
                         ttttatATTT.T.TTTT.G.TTT.T.TTGTTTTTTTTAtttt            <  1:6933943/36‑2 (MQ=2)
                         gtttttCGTT.T.TTTT.T.TTT.T.TGTTTTTTTTTTtttt            <  1:6520416/35‑2 (MQ=2)
                         gattttTGTT.T.TTTT.G.TTT.T.TTTTTTTTTTTTtttt            <  1:7456279/34‑2 (MQ=9)
                          tttttTGTT.G.TTTG.T.TTT.T.TGTGTTTTTGTTttttt           <  1:6699914/36‑3 (MQ=2)
                          tttttTCTT.G.TCTT.T.TTG.T.TTTTTTTTTTTTttttt           <  1:6680134/36‑3 (MQ=0)
                          tttttATTT.G.TTGT.T.TTT.T.TGTTTTTTTTTTttttt           <  1:7039604/36‑3 (MQ=2)
                          tttttAGTT.G.TCTT.G.TTT.T.TGTTTTTTTCTTTTTct           >  1:4688369/1‑35 (MQ=255)
                          tttttAGTT.G.TCTT.G.TTT.T.TGTTTTTTTCTATTTct           >  1:2475464/1‑35 (MQ=255)
                          gttttAATT.T.TTTT.T.TTG.T.TTTTTTTTTTTTTTTct           <  1:6837370/35‑2 (MQ=9)
                           ttttTGTT.G.GTTT.T.TTG.T.TTTTTTTTTTTTtttttt          <  1:7365647/36‑4 (MQ=2)
                           ttttAGTT.G.TCTT.G.TTT.T.TGTTTTTTTCTATTTCGc          <  1:257317/36‑1 (MQ=255)
                            tttCGTT.G.TTTT.T.TTTGT.TTTTTTTTTGTTtttttt          <  1:6953814/36‑4 (MQ=2)
                             ttAGTT.G.TCTT.G.TTT.T.TGTTTTTTTCTATTTCTCaa        >  1:7385047/1‑36 (MQ=255)
                              tAGTT.G.TCTT.G.TTT.T.TGTTTTTTTCTATTTCGCAat       <  1:4099861/36‑1 (MQ=255)
                              tAGTT.G.TCTT.G.TTT.T.TGTTTTTTTCTATTTCGCAat       >  1:4872804/1‑36 (MQ=255)
                               aGTT.G.TCTT.G.TTT.T.TGTTTTTTTCTATTTCGCAata      <  1:3901407/36‑1 (MQ=255)
                                gTT.G.TCTT.G.TTT.T.TGTTTTTTTCTATTTCGCAatat     <  1:4540145/36‑1 (MQ=255)
                                gTT.G.TCTT.G.TTT.T.TGTTTTTTTCTATTTCGCAatat     <  1:2821673/36‑1 (MQ=255)
                                 tt.G.TCTT.G.TTT.T.TGTTTGTTTCTATTTCGCAATAtt    <  1:6422616/36‑1 (MQ=255)
                                  t.G.TCTT.G.TTT.T.TGTTTTTTTCTATTTCGCAATATTa   <  1:154748/36‑1 (MQ=255)
                                  t.G.TCTT.G.TTT.T.TGTTTTTTTCTATTTCGCAATATTa   <  1:6840805/36‑1 (MQ=255)
                                  t.G.TCTT.G.TTT.T.TGTTTTTTTCTATTTCGCAATATTa   <  1:610406/36‑1 (MQ=255)
                                    g.TCTT.G.TTT.T.TGTTTTTTTCTATTTCGCAATATTaa  >  1:4409435/1‑36 (MQ=255)
                                    |                                        
TTATGGAATTACTGGTGGGAATGTTTTTTTTAGTT.G.TCTT.G.TTT.T.TGTTTTTTTCTATTTCGCAATATTAA  >  REL606/4462960‑4463030

Base quality scores:  ATCG  < 3 ≤  ATCG  < 7 ≤  ATCG  < 10 ≤  ATCG  < 24 ≤  ATCG  < 32 ≤  ATCG