Read alignment evidence...
  seq id position change freq score reads annotation genes product
*REL6063,535,0350T→C27.5% 10.751intergenic (‑104/‑46)ftsY/yhhFfused Signal Recognition Particle (SRP) receptor: membrane binding protein/conserved protein/predicted methyltransferase
Reads supporting (aligned to +/- strand):  new base (0/14):  ref base (20/17):  total (20/31)

ATGATGAAATAGTCTATCACTTAACTTAATTCACATCACCGCCTGCAAGTATGTGTTATCTGGCGGATTGA  >  REL606/3535000‑3535070
                                   |                                   
atgatgAAATAGTCTATCACTTAACTTAATTCACAt                                     <  1:4880844/36‑1 (MQ=255)
atgatgAAATAGTCTATCACTTAACTTAATTCACAt                                     <  1:6880477/36‑1 (MQ=255)
atgatgAAAAAGTCTATCACTTAACTTAATTCACAt                                     <  1:5376131/36‑1 (MQ=255)
aagatgaAATAGTCTATCACTTAACTTAATTCACAt                                     <  1:333092/34‑1 (MQ=255)
 tgatgaAATAGTCTATCACTTAACTTAATTCACATc                                    >  1:5096388/1‑36 (MQ=255)
  gatgaAATAGTCTATCACTTAACTTAATTCACATCa                                   >  1:3660290/1‑36 (MQ=255)
  gatgaAATAGTCTATCACTTAACTTAATTCACATCa                                   >  1:6743954/1‑36 (MQ=255)
    tgaGATAGTCTATCACTTAACTTAATTCACATCGcc                                 <  1:4152323/36‑1 (MQ=25)
     gaAATAGTCTATCACTTAACTTAATTCACATCACCg                                <  1:1082903/36‑1 (MQ=255)
     gaAATAGTCTATCACTTAACTTAATTCACATCACCg                                >  1:1328395/1‑36 (MQ=255)
     aaaaTAATCTATCACTTAACTTAATTCACATCACCg                                <  1:783802/35‑1 (MQ=255)
      aaaTAGTCTATCACTTAACTTAATTCACATCACCGc                               <  1:2166865/36‑1 (MQ=255)
      aaaTAGTCTATCACTTAACTTAATTCACATCACCGc                               <  1:5136441/36‑1 (MQ=255)
      aaaTAGTCTATCACTTAACTTAATTCACATCACCGc                               <  1:3914356/36‑1 (MQ=255)
      aaaTAGTCTATCACTTAACTTAATTCACATCACCGc                               <  1:323265/36‑1 (MQ=255)
       aaTAGTTTATCACTTTACTTAATTCACATCACCGcc                              <  1:7069932/36‑1 (MQ=25)
       aaTAGTCTATCACTTAACTTAATTCACATCACCGcc                              >  1:1985509/1‑36 (MQ=255)
        aTAGTCTATCACTTAACTTAATTCACATCACCGCCt                             <  1:1780047/36‑1 (MQ=255)
        aTAGTCTATCACTTAACTTAATTCACATCACCGCCt                             >  1:3264329/1‑36 (MQ=255)
        aTAGTCTATCACCTAACTTAATTCACATCACCGCCt                             >  1:2810530/1‑36 (MQ=255)
         tAGTCTATCACTTAACTTAATTCACATCACCGCCTg                            <  1:4031442/36‑1 (MQ=255)
           gTCTATCACTTAACTTAATTCACATCACCGCCTGCa                          >  1:7293814/1‑36 (MQ=255)
            tCTATCACTTAACTTAATTCACATCACCGCCTGCaa                         >  1:1244099/1‑36 (MQ=255)
             cTATCACTTAACTTAATTCACATCACCGCCTGCacg                        >  1:3975445/1‑34 (MQ=255)
             cTATCACTTAACTTAATTCACATCACCGCCCGCAAg                        >  1:6362541/1‑36 (MQ=255)
              atccACTTAACTTAATTCATACCCCCGCCTGCAAGt                       <  1:2767657/33‑1 (MQ=18)
               aTCACTTAACTTAATCCACATCACCGCCTGCAAGTa                      <  1:2255641/36‑1 (MQ=255)
                 ctcttaacttaaTCCACACCACCGCCTGCAAGTAtg                    <  1:2289945/34‑1 (MQ=25)
                 cgcttatcttaaTCCACACCACCGCCTGCAAGTAtg                    <  1:6002921/34‑1 (MQ=21)
                  acttaacttaATTCACATCACCGCCTGCAAGTAtgt                   >  1:7145932/1‑36 (MQ=255)
                  acttaacttaATTCACATCACCGCCTGCAAGTAtgt                   >  1:2513839/1‑36 (MQ=255)
                  acttaacttaATCCCCACCACCGCCTGCAAGTAtgt                   <  1:3571875/36‑1 (MQ=21)
                  acttaactaaATCCCCATCACCGCCTGCAAGTAtgt                   <  1:2825661/36‑1 (MQ=21)
                  actaaactaaATCCACACCACCGCCTGCAAGTAtgt                   <  1:990241/36‑1 (MQ=18)
                   cttaacttaaTTCACATCACCGCCTGCACGTAtgtg                  >  1:4752639/1‑36 (MQ=255)
                    ttaacttaaTTCACATCACCGCCTGCAAGTATgtgt                 >  1:397026/1‑36 (MQ=255)
                    ttaacttaaTTCACACCCCCGCCTGCAAGTATgtgt                 <  1:5032711/36‑1 (MQ=25)
                    ttaaattaaTACACATCACCGCCTGCAAGTATgtgt                 <  1:560110/36‑1 (MQ=25)
                      aactttaTTCACATCCCCGCCTGCAAGTATGTGTTa               <  1:4016307/36‑1 (MQ=25)
                      aacttaaTTCACATCCCCGCCTGCAAGTATGTGTTa               <  1:4711008/36‑1 (MQ=255)
                      aacttaaTTCACATCACCGCCTGCAAGTATGTGTTa               >  1:790955/1‑36 (MQ=255)
                        cttaaGCCACATCACCGCCTGCAAGTATGTGTTGTc             <  1:5636160/36‑1 (MQ=21)
                          taaTTCACACCGCCGCCTGCAAGTATGTGTTATCTg           <  1:2505764/36‑1 (MQ=25)
                          aaaTCCACACCCCCGCCTGCAAGTATGTGTTATCTg           <  1:7138194/35‑1 (MQ=21)
                            atccactTCCCCGCCTGCAAGTATGTGTTATCTGGc         <  1:7241678/33‑1 (MQ=21)
                            atccacaCCCCCGCCTGCAAGTATGTGTTATCTGGc         <  1:3324039/33‑1 (MQ=21)
                            acccacaCCCCCGCCTGCAAGTATGTGTTATCTGGc         <  1:2605847/33‑1 (MQ=21)
                            aTTCACATCACCGCCTGCAAGTATGTGTTATCTGGc         >  1:3669428/1‑36 (MQ=255)
                             ttCACACCACCGCCTGCAAGTATGTGTTATCTGGCg        <  1:7399962/36‑1 (MQ=255)
                             tccacaCCACCGCCTGCAAGTATGTGTTATCTGGCg        <  1:7269208/34‑1 (MQ=25)
                              acacaCCACCGCCTGCAAGTATGTGTTATCTGGCgg       <  1:3563817/35‑1 (MQ=255)
                                acaTCACCGCCTGCAAGTATGTGTTATCTAGCGGAt     >  1:1509974/1‑36 (MQ=255)
                                acaCCACCGCCTGCAAGTATGTGTTATCTGGCGGAt     <  1:1880384/36‑1 (MQ=255)
                                 caTCACCGCCTGCAAGTATGTGTTATCTGGCGGAtt    >  1:3901969/1‑36 (MQ=255)
                                  aTCACCGCCTGCAAGTATGTGTTATCTGGCGGATTg   >  1:1131355/1‑36 (MQ=255)
                                   tCACCGCCTGCAAGTATGTGTTATCTGGCGGATTGa  >  1:1178672/1‑36 (MQ=255)
                                   |                                   
ATGATGAAATAGTCTATCACTTAACTTAATTCACATCACCGCCTGCAAGTATGTGTTATCTGGCGGATTGA  >  REL606/3535000‑3535070

Base quality scores:  ATCG  < 3 ≤  ATCG  < 7 ≤  ATCG  < 13 ≤  ATCG  < 27 ≤  ATCG  < 32 ≤  ATCG