Read alignment evidence...
  seq id position change freq score reads annotation genes product
*REL606342,3450C→A22.2% 10.155F393L (TTC→TTAmhpA3‑(3‑hydroxyphenyl)propionate hydroxylase
Reads supporting (aligned to +/- strand):  new base (0/12):  ref base (33/9):  total (34/21)

CGCCAGTAAAACGCTACTTCCTCGAAATGCGCTTCAAGCCGATGCCGCAATATTACGGCGGTGCGCTG  >  REL606/342311‑342378
                                  |                                 
cgccAGTAAAACGCTACTTCCTCGAAATGCGCTTCa                                  <  1:5666816/36‑1 (MQ=255)
caccAGTAAAACGCTACTTCCTCGAAATGCGCATCa                                  >  1:5941420/3‑36 (MQ=25)
 gccAGTAAAACGCTACTTCCTCGAAATGCGCTTCaa                                 <  1:4341069/36‑1 (MQ=255)
 gccAGTAAAACGCTACTTCCTCGAAATGCGCTTCaa                                 >  1:7215859/1‑36 (MQ=255)
 gccAGTAAAACGCTACTTCCTCGAAATGCGCTTCaa                                 >  1:2314824/1‑36 (MQ=255)
  ccAGTAAAACGCTACTTCCTCGAAATGCGCTTCAAg                                >  1:6663697/1‑36 (MQ=255)
     gTAAACGGCTACTTCGTCGAAATGCGCTTCAAGCCg                             <  1:5049327/36‑1 (MQ=255)
      tAAAACGCTACTTCCTCGAAATGCGCTTCAAGCCGa                            >  1:2083201/1‑36 (MQ=255)
      aaaaaCGCTACTTCCTCGAAATGCGCTTCAAGCCGa                            <  1:381094/35‑1 (MQ=255)
       aaaaCGCTACTTCCTCGAAATGCGCTTCAAGCCGAt                           >  1:3124176/1‑36 (MQ=255)
        aaaCTCTACTTCCTCGAAATGCCCTTCAAGCCGATg                          <  1:1782926/36‑1 (MQ=25)
        aaaCGCTACTTCCTCGAAATGCGCTTCAAGCCGATg                          >  1:921056/1‑36 (MQ=255)
         aaCGCTACTTCCTCGAAATGCGCTTCAAGCCGATgt                         >  1:1237377/1‑35 (MQ=255)
         aaCGCTACTTCCTCGAAATGCGCTTCAAGCCGATGc                         >  1:6183006/1‑36 (MQ=255)
          aCGCTACTTCCTCGAAATGCGCTTCAAGCCGATGcc                        >  1:598933/1‑36 (MQ=255)
          aCGCTACTTCCTCGAAATGCGCTTCAAGCCGATGcc                        <  1:123574/36‑1 (MQ=255)
           cGCTACTTCCTCGAAATGCGCTTCAAGCCGATGCCg                       >  1:6347935/1‑36 (MQ=255)
           cGCTACTTCCTCGAAATGCGCTTCAAGCCGATGCCg                       >  1:3004924/1‑36 (MQ=255)
           cGCAACTCCCTCAAAATCCCCTTCAAGCCGATGCCg                       <  1:3159532/36‑1 (MQ=12)
            gCTGCTTCCTCGAAAGGCGCTTAAAGCCGATGCCGc                      <  1:5631794/36‑1 (MQ=21)
            gCTACTTCCTCGAAATGCGCTTCAAGCCGCTGCCGc                      >  1:7437196/1‑36 (MQ=255)
             cTACTTCCTCGAAATGCGCTTCAAGCCGATGCCGCa                     >  1:3522801/1‑36 (MQ=255)
             cTACTTCCTAGAAATGCGTTAAAAGCCGATGCCGCa                     <  1:455847/36‑1 (MQ=18)
              tACTTCCTCGAAATGCGCTTCAAGCCGATGCCGCaa                    >  1:1124901/1‑36 (MQ=255)
              tACTTCCCCGAAATCCGCTTAAAGCCGATGCCGCaa                    <  1:6545487/36‑1 (MQ=21)
               tcTTCCTCGAAAGGCGCTTAAAGCCGATGCCGCAat                   <  1:567900/35‑1 (MQ=21)
               ccTTCCTCAAAATGCGCTTCAAGCCGATGCCGCAat                   <  1:7143767/35‑1 (MQ=255)
               aCTTCCTCGAAATGCGCTTCAAGCCGATGCCGCAat                   >  1:2717525/1‑36 (MQ=255)
               aCTTCCTCGAAATGCGCTTCAAGCCGATGCCGCAat                   >  1:2650489/1‑36 (MQ=255)
                catCCTCGAAATCCGCTTAAAGCCGATGCCGCAata                  <  1:824473/34‑1 (MQ=21)
                 ttCCTCGAAATGCGCTTCAAGCCGATGCCGCAatat                 >  1:1661841/1‑36 (MQ=255)
                  tCCTCGAAATGCGCTTCAAGCCGATGCCGCAATAtt                >  1:753919/1‑36 (MQ=255)
                   ccTCGAAATGCGCTTCAAGCCGATGCCGCAATATTa               >  1:4755931/1‑36 (MQ=255)
                   ccTCGAAATGCGCTTCAAGCCGATGCCGCAATATTa               >  1:1589668/1‑36 (MQ=255)
                    ctagAAATGCCCTTAAAGCCGATGCCGCAATATTAc              <  1:1513209/33‑1 (MQ=21)
                    cacGAAATGCATTAAAAGCCGATGCCGCAATATTAc              <  1:3279600/34‑1 (MQ=17)
                    cTCGAAATGCGCTTCAAGCCGATGCCGCACTATTAc              >  1:7191878/1‑36 (MQ=255)
                    cTCGAAATGCGCTTCAAGCCGATGCCGCAATATTAc              >  1:5822933/1‑36 (MQ=255)
                    cTCGAAATGCGCTTCAAGCCGATGCCGCAATATTAc              >  1:7145773/1‑36 (MQ=255)
                    cTCGAAATGCGCTGCAAGCCGATGCCGTAATATTAc              >  1:6971090/1‑36 (MQ=25)
                     tCGAAATGCGCTTCAAGCCGATGCCGCAATATTAcg             >  1:3226048/1‑36 (MQ=255)
                     tCGAAATGCGCTTCAAGCCGATGCCGCAATATTAcg             >  1:6259607/1‑36 (MQ=255)
                     acaaaaTGCGCTTAAAGCCGATGCCGCAATATTAcg             <  1:158144/33‑1 (MQ=25)
                      cGAAATGCGCTTCAAGCCGATGCCGCAACATTAcgg            >  1:7027986/1‑36 (MQ=255)
                       gAAAGGCGCTTAAAGCCGATGCCGCAATATTAcggc           <  1:2168109/36‑1 (MQ=25)
                        aaaGGCGCTTCAAGCCGATGCCGCAATATTAcggcg          <  1:3114404/36‑1 (MQ=255)
                          aTGCGCTTCAAGCCGATGCCGCAATATTAcggtggg        >  1:4399386/1‑35 (MQ=255)
                          aTGCGCTTCAAGCCGATGCCGCAATATTAcggcggg        >  1:521725/1‑35 (MQ=255)
                          aTGCGCTTCAAGCCGATGCCGCAATACTAcgccggg        >  1:2186068/1‑35 (MQ=25)
                           tGCGCTTCAAGCCGATGCCGCAATATTAcggcgggg       >  1:6119688/1‑34 (MQ=255)
                           tGCGCTTCAAGCCGATGCCGCAATATTAcggcgggg       >  1:1388501/1‑34 (MQ=255)
                           tGCGCTTAAAGCCGATGCCGCAATATTACGGCGGTg       <  1:2016763/36‑1 (MQ=255)
                           tGCGCTT.AAGCCGATGCCGCAATATTAcggcggggg      >  1:488478/1‑33 (MQ=21)
                             gccTTAAAGCCGATGCCGCAATATTACGGCGGTgcg     <  1:6029902/34‑1 (MQ=25)
                              tcTTAAAGCCGATGCCTCAATATTACGGCGGTgcgc    <  1:453958/35‑1 (MQ=25)
                                tacAATCCTATTCCGCAATATTACGGCGGTGCGCTg  <  1:3255125/34‑1 (MQ=18)
                                tacAAGCCGATGCCGCAATATTACGGCGGTGCGCTg  <  1:5918680/34‑1 (MQ=255)
                                  |                                 
CGCCAGTAAAACGCTACTTCCTCGAAATGCGCTTCAAGCCGATGCCGCAATATTACGGCGGTGCGCTG  >  REL606/342311‑342378

Base quality scores:  ATCG  < 3 ≤  ATCG  < 6 ≤  ATCG  < 14 ≤  ATCG  < 28 ≤  ATCG  < 32 ≤  ATCG