Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	REL606	3,286,461	0	T→G	25.0%	10.2	45	L600F (TTA→TTC)	arcB	hybrid sensory histidine kinase in two‑component regulatory system with ArcA
Reads supporting (aligned to +/- strand):  new base (11/0):  ref base (1/32):  total (13/32)

TTCAGCACGTTAGCGGTTAAGGCCACCAGCGGCGGTAAATCCTCGCGCGGATAACGCTTCGTCAGTTCAC  >  REL606/3286426‑3286495                                    |                                   ttCAGCACGTTAGCGGTTAAGGCCACCAGCGGCGGt                                    <  1:2991268/36‑1 (MQ=255) ttCAGCACGTTAGCGGTTAAGGCCACCAGCGGCGGt                                    <  1:4520889/36‑1 (MQ=255) ttCAGCACGTTAGCGGTTAAGGCCACCAGCGGCGGt                                    <  1:348573/36‑1 (MQ=255)  tCAGCACGTTAGCGGTTAAGGCCACCAGCGGCGGTa                                   <  1:6390688/36‑1 (MQ=255)     gCACGTTAGCGGTTAAGGCCACCAGCGGCGGTAAAt                                <  1:831085/36‑1 (MQ=255)      ctcGTTGGCGGTTGAGGCCACCAGCGGCGGTAAATc                               <  1:1302477/34‑1 (MQ=21)       gtgTTAGCGGTTAAGGCCACCAGCGGCGGTAAATcc                              <  1:368537/34‑1 (MQ=255)       aCGTTAGCGGTTAAGGCCACCAGCGGCGGGGAATcc                              >  1:5831261/1‑36 (MQ=21)         cttAGCGGTTAAGGCCACCAGCGGCGCTAAATCCTc                            <  1:5833971/35‑1 (MQ=255)          ttAGCGGTTTAGGCCACCAGCGGCGGTAAATCCTcg                           <  1:2084448/36‑1 (MQ=255)          ttAGCGGTTAAGGCCACCAGCGGCGGTAAATCCTcg                           <  1:4478233/36‑1 (MQ=255)          ttAGCGGTTAAGGCCACCAGCGGCGGGGGATCCTct                           >  1:3461937/1‑35 (MQ=18)          ttAGCGGTTAAGGCCACCAGCGGCGGGAAATTCttg                           >  1:2091102/1‑34 (MQ=21)            ggCGGTTAAGGCCACCAGCGGCGGTAAATCCTcgcg                         <  1:1215043/35‑1 (MQ=255)            aGCGGTTAAGGCCACCAGCGGCGGTAAATCCTcgcg                         <  1:6014014/36‑1 (MQ=255)             gCGGTTAAGGCCACCAGCGGCGGTAAATCCTcgcgc                        <  1:3502155/36‑1 (MQ=255)             ctggTTAAGGCCACCAGCGGCGGTAAATCCTcgcgc                        <  1:174423/34‑1 (MQ=255)              cGGTTAAGGCCACCAGCGGCGGGAAATCCTcgcgcg                       >  1:3699748/1‑36 (MQ=255)               ggTTAGGGCCACCAGCGTCGGTAAATCCTCGCGCgg                      <  1:4786479/36‑1 (MQ=25)               ggTTAAGGCCACCAGCGGCGGGGAATTCTCCCCCgg                      >  1:1707457/1‑36 (MQ=9)               ggTTAAGGCCACCAGCGGCGGGGAATCCTcgcgccg                      >  1:2113118/1‑34 (MQ=21)               ggTTAAGGCCACCAGCGGCGGGAAAACCTcccgccg                      >  1:3913647/1‑34 (MQ=18)                gTTAAGGCCACCAGCGGCGGTAAATCCTCGCGCGGa                     <  1:2057119/36‑1 (MQ=255)                  tAAGGCCACCAGTGGCGGTAAATCCTCGCGCGGATa                   <  1:5371497/36‑1 (MQ=255)                  tAAGGCCACCAGCGGCGGGGAATCCCCGCGCGGGTa                   >  1:5355663/1‑36 (MQ=17)                  tAAGGCCACCAGCGGCGGGGAAAACTCGCGCGGGTa                   >  1:3213356/1‑36 (MQ=9)                  tAAGGCCACCAGCGGCGGCTCATCCCCGCCCggcca                   >  1:6328953/1‑33 (MQ=2)                      gCCACCAGCGGCGGGTAATACTCACGCGGATAACga               >  1:355957/1‑35 (MQ=18)                       ccatcaGCGGCGGTAAATCCTCGCGCGGATAACGCt              <  1:4818891/36‑1 (MQ=255)                       ccaccaGCGGCGGGGACTCCTGCGGGGGATAACGCt              >  1:3623521/1‑36 (MQ=2)                        caccaGCGGCGGTAAATCCTCGCGCGGATAACGCtt             <  1:5453562/36‑1 (MQ=255)                        caccaGCGGCGGTAAATCCTCGCGCGGATAACGCtt             <  1:2446552/36‑1 (MQ=255)                         accaGCGGCGGTAAATCCTCGCGCGGATAACGCTTc            <  1:3634322/36‑1 (MQ=255)                           caGCGGCGGTAAATCCTCGCGCGGATAACGCTTCGt          <  1:2939775/36‑1 (MQ=255)                           caGCAGCGGTAAATCCTCGCGCGGATAACGCTTCGt          <  1:6725169/36‑1 (MQ=255)                             gcggcgGTAAATCCTCGCGCGGATAACGCTTCGTCa        <  1:5140354/36‑1 (MQ=255)                             gcggcgGTAAATCCTCGCGCGGATAACGCTTCGTCa        <  1:5414556/36‑1 (MQ=255)                              cggcggTAAATCCTCGCGCGGATAACGCTTCGTCAg       <  1:276217/36‑1 (MQ=255)                              cggcggTAAATCCTCGCGCGGATAACGCTTCGTCAg       <  1:5966310/36‑1 (MQ=255)                              cggcggTAAATCCTCGCGCGGATAACGCTTCGTCAg       <  1:1653857/36‑1 (MQ=255)                               ggcggTAAATCCTCGCGCGGATAACGCTTCGTCAGt      <  1:4930189/36‑1 (MQ=255)                               ggcggTAAATCCTCGCGCGGATAACGCTTCGTCAGt      <  1:5882674/36‑1 (MQ=255)                                gcggTAAATCCTCGCGCGGATAACGCTTCGTCAGtt     <  1:4824718/36‑1 (MQ=255)                                 cggTAAATCCTCGCGCGGATAACGCTTCGTCAGTTc    <  1:4451268/36‑1 (MQ=255)                                  ggTAAATCCTCGCGGGGATAACGCTTCGTCAGTTCa   <  1:133577/36‑1 (MQ=255)                                  gctAAATCCTCGCGCGGATAACGCTTCGTCAGTTCa   <  1:2574522/34‑1 (MQ=255)                                   gTAAATCCTCGCGCGGATAACGCTTCGTCAGTTCAc  >  1:2165107/1‑36 (MQ=255)                                   gTAAATCCTCGCGCGGATAACGCTTCGTCAGTTCAc  <  1:2192376/36‑1 (MQ=255)                                   gTAAATCCTCGCGCGGATAACGCTTCGTCAGTTCAc  <  1:1164721/36‑1 (MQ=255)                                    |                                   TTCAGCACGTTAGCGGTTAAGGCCACCAGCGGCGGTAAATCCTCGCGCGGATAACGCTTCGTCAGTTCAC  >  REL606/3286426‑3286495 Base quality scores:  ATCG  < 3 ≤  ATCG  < 6 ≤  ATCG  < 13 ≤  ATCG  < 27 ≤  ATCG  < 32 ≤  ATCG