Read alignment evidence...
  seq id position change freq score reads annotation genes product
*REL6063,176,1310T→C29.4% 11.351E73G (GAA→GGA) uxaAaltronate hydrolase
Reads supporting (aligned to +/- strand):  new base (0/15):  ref base (23/13):  total (23/28)

TCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGTTCCCCCGCCGCAATATCCGCCAATGCATAACCAATC  >  REL606/3176096‑3176166
                                   |                                   
tCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGtt                                     <  1:267099/36‑1 (MQ=255)
 cAGATTCGTGCGCGTATTGTGGGCGTGAACGTGTTc                                    >  1:4454691/1‑36 (MQ=255)
  aGATTCGTGCGCGTATTGTGGGCGTGAACGTGTTcc                                   <  1:2391918/36‑1 (MQ=255)
  aGATGCGTGCGTGTATTGTGGGCGTGAACGTGTTcc                                   <  1:6876198/36‑1 (MQ=25)
   gATTCGTGCGCGTATTGTGGGCGTGAACGTGTTccc                                  <  1:1341961/36‑1 (MQ=255)
     ttCGTGCGCGTATTGTGGGCGTGAACGTGTTccccc                                <  1:3498051/36‑1 (MQ=255)
      tCGTGGGGGTATTGTGGGCGTGAACGTGTTCCCccg                               <  1:3663220/36‑1 (MQ=25)
      tCGTGCGCGTATTGTGGGCGTGAACGTGTTaccact                               >  1:6816691/1‑33 (MQ=25)
      tCGTGCGCGTATTGTGGGCGTGAACGTGTTCCCccg                               >  1:6517065/1‑36 (MQ=255)
      tCGTGCGCGTATTGTGGGCGTGAACGTGTTCCCccg                               <  1:1409083/36‑1 (MQ=255)
       cGTGCGCGTATTGTGGGCGTGAACGTGTTTCCccgc                              <  1:4546203/36‑1 (MQ=255)
        gTGCGCGTATTGTGGGCGTGAACGTGTTCCCccgcc                             <  1:7100618/36‑1 (MQ=255)
         tGCGCGTATTGTGGGCGTGAACGTGTTCCCccgccg                            >  1:5477421/1‑36 (MQ=255)
          gcgcTTTTTGTGGGCGTGAACGTGTTCCCCcgccgc                           <  1:1999818/36‑1 (MQ=25)
          gcgcGTATTGTGGGCGTGAACGTGTTCCCCcgccgc                           >  1:7372279/1‑36 (MQ=255)
            gcgTATTGTGGGCGTGAACGTGTTCCCCCGCCGCaa                         >  1:3552583/1‑36 (MQ=255)
             cttATTGTGGGCGTGAACGTGTTCCCCCGCCGCAat                        <  1:2751231/34‑1 (MQ=255)
             cttATTGGGGGCGTGAACGTGTCCCCCCGCCGCAat                        <  1:5424862/34‑1 (MQ=21)
             cgTTTTGTGGGCTTGACCGTTTCCCCCCGCCGCAat                        <  1:7369154/36‑1 (MQ=12)
             cgTATTGTGGGCGTGAACGTGTTCCCCCGCCGCAat                        >  1:968648/1‑36 (MQ=255)
              gTATTGTGGGCGTGAACGTGTTCCCCCGCCGCAata                       >  1:4303513/1‑36 (MQ=255)
              gTATTGTGGGCGTGAACGTGTTCCCCCGCCGCAata                       >  1:2700226/1‑36 (MQ=255)
               tATTGTGGGCGTGAACGTGTTCCCCCGCCGCAatat                      >  1:7343383/1‑36 (MQ=255)
               tATTGTGGGCGTGAACGTGTTCCCCCGCCGCAatat                      <  1:2583316/36‑1 (MQ=255)
                tttGTGGGCGTGAACGTGTCCCCCCGCCGCAATATc                     <  1:3171981/35‑1 (MQ=25)
                 ttGTGGGCGTGAGCGTGTCCCCCCGCCGCAATATcc                    <  1:2882492/36‑1 (MQ=25)
                 ttGTGGGCGTGAACGTGTTCCCCCGCCGCAATATcc                    >  1:1921325/1‑36 (MQ=255)
                 ttGTGGGCGTAAACGTGTCCCCCCGCCGCAATATcc                    <  1:3556006/36‑1 (MQ=25)
                 ttGTGGGCGGGACCGTGTTCCCCCGCCGCAATATcc                    <  1:3374331/36‑1 (MQ=21)
                 ttGGGGGCGTGAACGTGTCCCCCCGCCGCAATATcc                    <  1:5027939/36‑1 (MQ=25)
                  tgtgGGCGTGAACGTGTTCCCCCGCCGCAATATCCg                   >  1:522388/1‑36 (MQ=255)
                  tgtgGGCGAGAACGTTTCCCCCCGCCGCAATATccc                   <  1:3323500/36‑2 (MQ=21)
                  tgggggCGTGAACGTTCCCCCCCGCCGCAATATCCg                   <  1:6556631/33‑1 (MQ=18)
                  tgggggCGGAAACGTGCTCCCCCGCCGCAATATCCg                   <  1:6715912/33‑1 (MQ=18)
                   gtgGGCGTGACGGTGTCCCCCCGCCGCAATATCCGc                  <  1:1293919/36‑1 (MQ=21)
                   gtgGGCGTGAACGTGTTCCCCCGCCGCAATATCCGc                  >  1:7177010/1‑36 (MQ=255)
                    ggggGGTGATCGTGTCCCCCCGCCGCAATATCCGcc                 <  1:5657962/35‑1 (MQ=21)
                    ggggCGTGACCGTGTCCCCCCGCCGCAATATCCGcc                 <  1:2718179/35‑1 (MQ=25)
                      ggCGTGAACGTGTTCCCCCGCCGCAATATCCGCCaa               >  1:2327755/1‑36 (MQ=255)
                        cGTGAACGTGTTCCCCCGCCGCACTATCCGCCAATg             >  1:333190/1‑36 (MQ=255)
                        cGTGAACGTGTTCCCCCGCCGCAATATCCGCCAATg             >  1:5528136/1‑36 (MQ=255)
                         gaaaaCGTGTCCCCCCGCCGCAATATCCGCCAATGc            <  1:6300469/33‑1 (MQ=25)
                            accGTGTCCCCCCGCCGCAATATCCGCCAATGCATa         <  1:6484437/34‑1 (MQ=25)
                            aaCGTGTTCCCCCGCCGCAATATCCGCCCATGCATa         >  1:2687951/1‑36 (MQ=255)
                            aaCGTGCCCCCCCGCCGCAATATCCGCCAATGCATa         <  1:2420270/36‑1 (MQ=25)
                            aaCGCGATCCCCCGCCGCAATATCCGCCAATGCATa         >  1:4957354/1‑36 (MQ=25)
                             aCGTGTTCCCCCGCCGCAATATCCGCCAAAGCATaa        >  1:1025023/1‑36 (MQ=255)
                               gtgtTCCCCCGCCGCAATATCCGCCAATGCATAAcc      >  1:2712468/1‑36 (MQ=255)
                               gtgtCCTCCCGCCGCAATATCCGCCAATGCATAAcc      <  1:4368406/36‑1 (MQ=25)
                                tgtTCCCCCGCCGCAATATCCGCCAATGCATAACCa     >  1:468147/1‑36 (MQ=255)
                                 gtTCCCCCGCCGCAATATCCGCCAATGCATAACCaa    >  1:763961/1‑36 (MQ=255)
                                 gtTCCCCCGCCGCAATATCCGCCAATGCATAAACaa    >  1:3886402/1‑36 (MQ=255)
                                  ttCCCCCGCCGCAATATCCGCCAATGCATAACCAAt   >  1:5604202/1‑36 (MQ=255)
                                  ttCCCCCGCCGCAATATCCGCCAATGCATAACCAAt   >  1:2647766/1‑36 (MQ=255)
                                   tCCCCCGCCGCAATATCCGCCAATGCATCACCAATc  >  1:4393607/1‑36 (MQ=255)
                                   |                                   
TCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGTTCCCCCGCCGCAATATCCGCCAATGCATAACCAATC  >  REL606/3176096‑3176166

Base quality scores:  ATCG  < 3 ≤  ATCG  < 5 ≤  ATCG  < 12 ≤  ATCG  < 26 ≤  ATCG  < 32 ≤  ATCG