Read alignment evidence...
  seq id position change freq score reads annotation genes product
*REL6062,304,3690A→G28.3% 10.660E253G (GAA→GGA) yfaDhypothetical protein
Reads supporting (aligned to +/- strand):  new base (17/0):  ref base (16/27):  total (33/27)

GATTAATGACTATTGCTGAACGGCTACGGCA.GGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATG  >  REL606/2304336‑2304404
                                  |                                   
gaTTAATGACTATTGCTGAACGGCTACGGCA.GGAAg                                   <  1:7164490/36‑1 (MQ=255)
  ttAATGACTCTTGCTGAACGGCTACGGCA.CGGAggg                                 >  1:4464207/1‑36 (MQ=21)
  ttAATGACTATTGCTGAACGGCTACGGCA.GGGAggg                                 >  1:4245574/1‑36 (MQ=255)
   tAATGACTATTGCTGAACGGCTACGGCA.GGGAgggg                                >  1:5102010/1‑35 (MQ=255)
   tAATGACTATTGCTGAACGGCTACGGCA.GGGAgagg                                >  1:4313245/1‑33 (MQ=25)
   tAATGACTATTGCTGAACGGCTACGGCA.GGAAGGGc                                <  1:4650350/36‑1 (MQ=255)
    aaTGACTATTGCTGAACGGCTACGGCAGGGAAgggg                                >  1:5276565/1‑35 (MQ=21)
    aaTGACTATTGCTGAACGGCTACGGCA.GGGAggggc                               >  1:1829033/1‑34 (MQ=25)
     aTGACTATTGCTGAACGGCTACGGCA.GGAAGGGCAt                              <  1:5764074/36‑1 (MQ=255)
     aTGACTAGTGCTGAACGGCTACGGCA.GGAAGGGCAt                              <  1:3939447/36‑1 (MQ=255)
      tGACTATTGCTGAACGGCTACGGCA.GGGAGGGcgta                             >  1:5702580/1‑33 (MQ=25)
      tGACTATTGCTGAACGGCTACGGCA.GGGAGGGCacc                             >  1:308825/1‑34 (MQ=25)
      tGACTATTGCTGAACGGCTACGGCA.GGAAGGGCATc                             <  1:4925791/36‑1 (MQ=255)
       gACTATTGCTGAACGGCTACGGCA.GGAAGGGCATCa                            <  1:2030487/36‑1 (MQ=255)
       gACTATTGCTGAACGGCTACGGCA.GGAAGGGCATCa                            <  1:6312548/36‑1 (MQ=255)
        aCTATTGCTGAACGGCTACGGGA.GGGAGGGCATCaa                           >  1:6914225/1‑36 (MQ=25)
        aCTATTGCTGAACGGCTACGGCA.GGAAGGGCATCaa                           <  1:6424707/36‑1 (MQ=255)
         cTATTGCTGAACGGCTACGGCA.GGGAGGGCATCaaa                          >  1:4518435/1‑36 (MQ=255)
         cTATTGCTGAACGGCTACGGCA.GGAAGGGCCTCaaa                          >  1:2000109/1‑36 (MQ=255)
          tATTGCTGAACGGCTACGGCA.GGAAGGGGAACaaaa                         >  1:4078849/1‑35 (MQ=21)
          tATTGCTGAACGGCTACGGCA.GGAAGGGCCTCaaca                         >  1:1978754/1‑34 (MQ=25)
          tATTGCTGAACGGCTACGGCA.GGAAGGGCATCAAAt                         <  1:1209902/36‑1 (MQ=255)
           aTTGCTGAACGGCTACGGCA.GGGAGGGCATCAAAtt                        >  1:5248916/1‑36 (MQ=255)
           aTTGCTGAACGGCTACGGCA.GGAAGGGCATCAAAtt                        <  1:2751394/36‑1 (MQ=255)
             tGCTGAACGGCTACGGCA.GGGAGGGGATCAAATtgg                      >  1:846539/1‑36 (MQ=25)
             tGCTGAACGGCTACGGCA.GGGAGGGCATCAAAtttg                      >  1:813142/1‑34 (MQ=37)
             tGCTGAACGGCTACGGCA.GGGAGGGCACCAAAAtgg                      >  1:2533687/1‑36 (MQ=21)
             tGCTGAACGGCTACGGCA.GGAAGGGCATCAAATtgg                      <  1:601214/36‑1 (MQ=255)
               cTGAACGGCTACGGCA.GGAGGGGGATCAAATtgggt                    >  1:577083/1‑34 (MQ=21)
               cTGAACGGCTACGGCA.GGAAGGGGATCAAAAtggct                    >  1:2017575/1‑36 (MQ=25)
                tGAACGGCTACGGCA.GGGAGGGCATCAAATtgggtg                   >  1:1622293/1‑36 (MQ=25)
                tGAACGGCTACGGCA.GGAAGGGGGTCAAATaggctg                   >  1:4119494/1‑36 (MQ=21)
                tGAACGGCTACGGCA.GGAAGGGGCTCAAATaggttg                   >  1:4114069/1‑36 (MQ=18)
                tGAACGGCTACGGCA.GGAAGGGGATCAAAAtggctg                   >  1:5020616/1‑36 (MQ=25)
                tGAACGGCTACGGCA.GGAAGGGCATCAAATtggctg                   <  1:291985/36‑1 (MQ=255)
                 gAACGGCTGCGGCA.GGAAGGGCATCAAATtggctgg                  <  1:2530564/36‑1 (MQ=255)
                  aaCGGCTACGGCA.GGAAGGGCATCAAATtggctgnc                 <  1:3743407/36‑1 (MQ=255)
                  aaCGGCTACGGCA.GGAAGGGCATCAAATtggctggc                 <  1:2678427/36‑1 (MQ=255)
                   aCGGCTACGGCA.GGGAGAGGATTCAATttgctgggg                >  1:4098436/1‑34 (MQ=9)
                    cGGCTACGGCA.GGGAGGGGATCAAAAttgctgggcg               >  1:4773704/1‑33 (MQ=12)
                     ggCTACGGCA.GGGAGGGCATCAAATTGGCTGGCGgg              >  1:2691143/1‑36 (MQ=25)
                      gCTACGGCA.GGAAGGGCATCAAATTGGCTGGCAGGa             <  1:2742006/36‑1 (MQ=255)
                      gCTACGGCA.GGAAGGGCATCAAATTGGCTGGCAGGa             <  1:7285743/36‑1 (MQ=255)
                       cTACGGCA.GGAAGGGCATCAAATTGGCTGGGAggga            >  1:7106989/1‑34 (MQ=25)
                       cTACGGCA.GGAAGGGCATCAAATTGGCTGGCAGGaa            <  1:1258479/36‑1 (MQ=255)
                         aCGGCA.GGAAGGGCATCAAATTGGCTGGCAGGAAgg          <  1:837117/36‑1 (MQ=255)
                           ggCA.GGAAGGGCATACAACTGGCTGCCAGGAATGTa        >  1:3807307/1‑36 (MQ=12)
                            gCA.GGAAGGGCATCAAATTGGCTGGCAGGAAGGTAt       >  1:1571205/1‑36 (MQ=255)
                             cA.GGAAGGGCATCAAATTGGCTGGCAGGAAGGTATg      >  1:4663203/1‑36 (MQ=255)
                             cA.GGAAGGGCATCAAATTGGCTGGCAGGAAGGTATg      <  1:707078/36‑1 (MQ=255)
                             cA.GGAAGGGCATCAAATTGGCTGGCAGGAAGGTATg      <  1:1953119/36‑1 (MQ=255)
                             cA.GGAAGGGCATCAAATTGGCTGGCAGGAAGGGATg      >  1:4503277/1‑36 (MQ=255)
                              a.GGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGc     >  1:3023703/1‑36 (MQ=255)
                              a.GGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGc     <  1:6927676/36‑1 (MQ=255)
                              a.GGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGc     <  1:5372149/36‑1 (MQ=255)
                                ggAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCa    <  1:694079/36‑1 (MQ=255)
                                ggAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCa    <  1:2934807/36‑1 (MQ=255)
                                agAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCa    <  1:2809055/35‑1 (MQ=255)
                                 gAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCAt   >  1:4405108/1‑36 (MQ=255)
                                 gAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCAt   <  1:2261811/36‑1 (MQ=255)
                                  aaGGGCATCAAATTGGCTGGCAGGAAGGTATGCATg  >  1:6466146/1‑36 (MQ=255)
                                  aaGGGCATCAAATTGGCTGGCAGGAAGGTATGCATg  >  1:6367855/1‑36 (MQ=255)
                                  aaGGGCATCAAATTGGCTGGCAGGAAGGTATGCATg  <  1:5345270/36‑1 (MQ=255)
                                  aaGAGCAACAAATTGGCTGGCAGGAAGGTATGCATg  <  1:2729522/36‑1 (MQ=21)
                                  |                                   
GATTAATGACTATTGCTGAACGGCTACGGCA.GGAAGGGCATCAAATTGGCTGGCAGGAAGGTATGCATG  >  REL606/2304336‑2304404

Base quality scores:  ATCG  < 3 ≤  ATCG  < 5 ≤  ATCG  < 11 ≤  ATCG  < 26 ≤  ATCG  < 32 ≤  ATCG