Read alignment evidence...
  seq id position change freq score reads annotation genes product
*REL6061,854,3850C→G30.8% 11.739A83G (GCG→GGG) yeaOhypothetical protein
Reads supporting (aligned to +/- strand):  new base (12/0):  ref base (10/17):  total (22/17)

CTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCT  >  REL606/1854351‑1854420
                                  |                                   
cTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCg                                    <  1:1604548/36‑1 (MQ=255)
 tGGCGCAACACGAGCAAGAAGGAAAGCGTCTGGGgg                                   >  1:1248485/1‑36 (MQ=255)
 tGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGGgg                                   >  1:5024859/1‑36 (MQ=255)
 tGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCgg                                   <  1:7013997/36‑1 (MQ=255)
  ggCGCAACACGAGCAAGAAGGAAAGCGGCTGGGggg                                  >  1:6433422/1‑35 (MQ=255)
   gcgcGACACGAGCAAGATGGAGAGCGGCTGGCGGAc                                 <  1:1941746/36‑1 (MQ=21)
   gcgcAACACGAGCAAGAAGGAAAGCGGCTGGCGGAc                                 <  1:4368325/36‑1 (MQ=255)
    cgcAACACGAGCAAGAAGGAAAGCGGCTGGGgggga                                >  1:851581/1‑33 (MQ=25)
    cgcAACACGAGCAAGAAGGAAAGCGGCTGGCGGACa                                <  1:2051126/36‑1 (MQ=255)
     gcAACACGAGCAAGAAGGAAAGCGGCTGGCGGGCaa                               >  1:1121653/1‑35 (MQ=255)
      cAACACGAGCAAGAAGGAAAGCGGCTGGGGGGCAtg                              >  1:5409057/1‑35 (MQ=25)
      cAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATc                              <  1:6212449/36‑1 (MQ=255)
      cAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATc                              <  1:6339985/36‑1 (MQ=255)
      cAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATc                              <  1:4551225/36‑1 (MQ=255)
      cAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATc                              <  1:993970/36‑1 (MQ=255)
         aacGAGCAAGAAGGAAAGCGGCTGGCGGACATCGcc                           <  1:390780/35‑1 (MQ=255)
          acGAGCAAGAAGGCAAGCGGCTGGCGGACATCGCCa                          <  1:6373396/36‑1 (MQ=255)
           cGAGCAAGAAGGAAAGCGGCTGGGGGGGATCGccca                         >  1:5758314/1‑34 (MQ=21)
           cGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCaa                         <  1:3336134/36‑1 (MQ=255)
           cGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCaa                         <  1:2589036/36‑1 (MQ=255)
            gAGCAAGAAGGAAAGCGGCTGGGGGGGAACGCCaca                        >  1:7168195/1‑34 (MQ=18)
            gAGCAAGAAGGAAAGCGGCTGGGGGAGACCGCCcaa                        >  1:5175590/1‑36 (MQ=18)
            gAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCaaa                        <  1:4984831/36‑1 (MQ=255)
             agaaagaagGAAAGCGGCTGGCGGACATCGCCaaaa                       <  1:2449932/33‑1 (MQ=255)
              gCAAGAAGGAAAGCGGCTGGCGGACATCGCCaaaaa                      <  1:4772463/36‑1 (MQ=255)
                aagaagGAAAGCGGCTGGGGGGCATCGTCCAAAAAc                    >  1:3520629/1‑36 (MQ=18)
                 agaagGAAAGCGGCTGGGCGGGATCGCCcaaaaaaa                   >  1:5545739/1‑34 (MQ=9)
                  gaagGAAAGCGGCTGGGGGGCAATGCCcaaaaaaaa                  >  1:2823580/1‑33 (MQ=9)
                    agGAAAGCGGCTGGGGGACAATGCCAAAAAACaaac                >  1:293884/1‑33 (MQ=18)
                    agGAAAGCGGCTGGCGGGCCTTGCCCAAAAACAGcc                >  1:1811142/1‑36 (MQ=18)
                    agGAAAGCGGCTGGCGGACACCGCCAAAAAAAAGcc                >  1:4697105/1‑36 (MQ=25)
                     ggAAAGCGGCTGGCGGGCACCGCCCAAAAACAACCg               >  1:1065955/1‑36 (MQ=18)
                      gAAAGCGGCTGGCGGGCACCCCCAAAAAAAAACCGc              >  1:5553958/1‑36 (MQ=12)
                        aaGCGGCTGGCGGGCATCGGAAAAAAACAAGCGCTg            >  1:4615208/1‑36 (MQ=12)
                        aaGCGGCTGGCGGACATCGCCAAAAAAAAACCggcg            >  1:316345/1‑33 (MQ=18)
                            ggCGGGCGAACAGCACAAAAACCCAGCCGCTGAccc        <  1:3194373/36‑1 (MQ=2)
                              cTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTg      >  1:6071815/1‑36 (MQ=255)
                              cTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTg      >  1:4937358/1‑36 (MQ=255)
                              cTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTg      >  1:3265316/1‑36 (MQ=255)
                                  cGGACATCGCCAAAAAACAGCCGCTGACCCTGctct  >  1:5442982/1‑36 (MQ=255)
                                  |                                   
CTGGCGCAACACGAGCAAGAAGGAAAGCGGCTGGCGGACATCGCCAAAAAACAGCCGCTGACCCTGCTCT  >  REL606/1854351‑1854420

Base quality scores:  ATCG  < 3 ≤  ATCG  < 5 ≤  ATCG  < 10 ≤  ATCG  < 26 ≤  ATCG  < 32 ≤  ATCG