Read alignment evidence...
  seq id position change freq score reads annotation genes product
*REL6061,499,2340A→G100.0% 0.7 6E384G (GAG→GGG) ECB_01413conserved hypothetical protein
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (5/0):  ref base (1/0):  total (6/0)

GCAGAGCGCTGTCGTGACAGGACCGGCAGGCGAGGAAATCTTCTGCGACGAACATGGTCGCGT  >  REL606/1499202‑1499264
                                |                              
gCAGAGCGCTGTCGTGACAGGACCGGCAGGCGGggg                             >  1:2336818/1‑35 (MQ=0)
 cAGAGCGCTGTCGTGACAGGACCGGCAGGCGGggga                            >  1:6430767/1‑34 (MQ=1)
         tGTCGTGACAGGACCGGCAGGCGGGGAAAttttttt                    >  1:172848/1‑33 (MQ=1)
          gTCGTGACAGGACCGGCAGGGGGGGGAATCTTTTgg                   >  1:4506233/1‑35 (MQ=1)
             gTGACAGGACCGGCAGGGGGGGGAATCTTCTGtgag                >  1:3837143/1‑35 (MQ=1)
                           aGGCGAGGAAATCTTCTGCGACGAACATGGAcgtgg  >  1:6563012/1‑33 (MQ=1)
                                |                              
GCAGAGCGCTGTCGTGACAGGACCGGCAGGCGAGGAAATCTTCTGCGACGAACATGGTCGCGT  >  REL606/1499202‑1499264

Base quality scores:  ATCG  < 3 ≤  ATCG  < 4 ≤  ATCG  < 7 ≤  ATCG  < 20 ≤  ATCG  < 32 ≤  ATCG