Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	REL606	1,110,603	0	C→T	100.0%	1.1	2	G258D (GGC→GAC)	insF‑1	putative transposase for IS3
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (1/0):  ref base (1/0):  total (2/0)

TTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCG  >  REL606/1110573‑1110622                               |                    tgattttCAAATTGTTCCGGACTGAGACCGTCtcac                >  1:4160900/4‑36 (MQ=17)               ttCCGGACTGAGGCCGCCACACCAACTGTgtcgtcg  >  1:3590277/1‑36 (MQ=1)                               |                    TTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCG  >  REL606/1110573‑1110622 Base quality scores:  ATCG  < 3 ≤  ATCG  < 11 ≤  ATCG  < 19 ≤  ATCG  < 26 ≤  ATCG  < 32 ≤  ATCG