Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	178	1,350	A→G	intergenic (–/–)	– / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	178	1,350	0	A	G	100.0%	162.4 / NA	46	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/26);  total (20/26)

GAAAGCCGGTATCGCGTCATTCTGTCCCTATAACATTGGCCCTGGTAAGTGTTTTCCGTCGACGTTTTATAAGCGGCTTAATGCCGGTGATCGTAAAGGCGCATGCGAGGCGATTCGCTGGTGGATAAAGGACGGTGGACGTGATTGCCGCATTCGTTCAAATAATTGTTACGGTCAGGTTATTCGTCGTGAC  >  178/1254‑1446                                                                                                 |                                                                                                 gAAAGCGGGTATAGCGTCATTTTGTCCCTATAACATTGGCCCCGGTAAGTGTTTCCCGTCGACGTTTTATAAGCGGCTGAATGCCGGTGATCGTAAgggtg                                                                                              >  2:1325789/1‑99 (MQ=255)    aGCGGGTATAGCGTCATTTTGTCCCTATAACATTGGCCCCGGTAAGTGTTTCCCGTCGACGTTTTATAAGCGGCTGAATGCCGGTGATCGTAAGGGTGCAt                                                                                           <  1:1241588/101‑1 (MQ=255)     gcgggTATAGCGTCATTTTGTCCCTATAACATTGGCCCCGGTAAGTGTTTCCCGTCGACGTTTTATAAGCGGCTGAATGCCGGTAATCGTAAGGGTGCATg                                                                                          <  1:1492219/98‑1 (MQ=255)       gggTATAGCGTCATTTTGTCCCTATAACATTGGCCCCGGTAAGTGTTTCCCGTCGACGTTTTATAAGCGGCTGAATGCCGGTGATCGTAAGGGTGCATGCg                                                                                        <  1:313041/100‑1 (MQ=255)          tataGCGTCATTTTGTCCCTATAACATTGGCCCCGGTAAGTGTTTCCCGTCGACGTTTTATAAGCGGCTGAATGCCGGTGATCGTAAGGGTGCATGCGAgg                                                                                     <  2:27652/101‑1 (MQ=255)               cgTCATTTTGTCCCTATAACATTGGCCCCGGTAAGTGTTTCCCGTCGACGTTTTATAAGCGGCTGAATGCCGGTGATTGTAAGGGTGCATGCGAGGCGAtt                                                                                >  1:1435772/1‑101 (MQ=255)               cgTCATTTTGTCCCTATAACATTGGCCCCGGTAAGTGTTTCCCGTCGACGTTTTATAAGCGGCTGAATGCCGGTGATCGTAAGGGTGCATGCGAGGCGAtt                                                                                >  2:1579656/1‑101 (MQ=255)               cgTCATTTTGTCCCTATAACATTGGCCCCGGTAAGTGTTTCCCGTCGACGTTTTATAAGCGGCTGAATGCCGGTGATCGTAAGGGTGCATGCGAGGCGAtt                                                                                >  2:177155/1‑101 (MQ=255)                     tttGTCCCTATAACATTGGCCCCGGTAAGTGTTTCCCGTCGACGTTTTATAAGCGGCTGAATGCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCtgg                                                                          >  1:1119698/3‑101 (MQ=255)                      ttGTCCCTATAACATTGGCCCTGGTAAGTGTTTCCCGTCGACGTTTTATAAGCGGCTGAATGCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCtggt                                                                         <  2:622556/100‑1 (MQ=255)                        gTCCCTATAACATTGGCCCCGGTAAGTGTTTCCCGTCGACGTTTTATAAGCGGCTGAATGCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCtggtgg                                                                       <  1:1439104/101‑1 (MQ=255)                         tCCCTATAACATTGGCCCCGGTAAGTGTTTCCCGTCGACGTTTTATAAGCGGCTGAATGCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGa                                                                      >  2:33228/1‑101 (MQ=255)                             tataACATTGGCCCCGGTAAGTGTTTCCCGTCGACGTTTTATAAGCGGCTGAATGCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGATaaa                                                                  <  1:1676492/101‑1 (MQ=255)                              ataACATTGGCCCCGGTAAGTGTTTCCCGTCGACGTTTTATAAGCGGCTGAATGCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGATaaaa                                                                 >  1:280073/1‑100 (MQ=255)                                aaCATTGGCCCCGGTAAGTGTTTCCCGTCGACGTTTTATAAGCGGCTGAATGCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGATAAAAGa                                                               >  2:570765/1‑101 (MQ=255)                                   aTTGGCCCCGGTAAGTGTTTCCCGTCGACGTTTTATAAGCGGCTGAATGCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGATAAAAGATgg                                                            <  2:1525422/101‑1 (MQ=255)                                   aTTGGCCCCGGTAAGTGTTTCCCGTCGACGTTTTATAAGCGGCTGAATGCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGATAAAAGATgg                                                            >  1:752757/1‑101 (MQ=255)                                   aTTGGCCCCGGTAAGNGTTTCCCGTCGACGTTTTATAAGCGGCTGAATGCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGATAAAAGATgg                                                            >  2:414822/1‑101 (MQ=255)                                       gCCCCGGTAAGTGTTTCCCGTCGACGTTTTATAAGCGGCTGAATGCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGATAAAAGAtggtggg                                                        <  2:1234647/101‑2 (MQ=255)                                        ccccGGTAAGTGTTTCCCGTCGACGTTTTATAAGCGGCTGAATGCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGATAAAAGAtggtgggc                                                       >  2:1018435/1‑99 (MQ=255)                                             gTAAGTGTTTCCCGTCGACGTTTTATAAGCGGCTGAATGCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGATAAAAGATGGTGGGCGCGAt                                                  <  1:274276/101‑1 (MQ=255)                                              tAAGTGTTTCCCGTCGACGTTTTATAAGCGGCTGAATGCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGATAAAAGATGGTGGGCGCGAtt                                                 <  2:1637059/101‑1 (MQ=255)                                               aattgtTTCCCGTCGACGTTTTATAAGCGGCTGAATGCCGGTGATCGTAAGGGTGCAAGCGAGGCGATTCGCTGGTGGATAAAAGATGGTGGGCGCGATTg                                                <  1:603354/98‑1 (MQ=255)                                                       cccGTCGACGTTTTATAAGCGGCTGAATGCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGATAAAAGATGGTGGGCGCGATTGCCGCAtac                                        <  2:1433044/100‑3 (MQ=255)                                                        ccGTCGACGTTTTATAAGCGGCTGAATGCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGATAAAAGATGGTGGGCGCGATTGCCGCATACg                                       >  1:359684/1‑101 (MQ=255)                                                          gTCGACGTTTTATAAGCGGCTGAATGCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGATAAAAGATGGTGGGCGCGATTGCCGCATACGtt                                     >  2:81095/1‑101 (MQ=255)                                                          gTCGACGTTTTATAAGCGGCTGAATGCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGATAAAAGATGGTGGGCGCGATTGCCGCATACGtt                                     >  1:1360889/1‑101 (MQ=255)                                                           tCGACGTTTTATAAGCGGCTGAATGCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGATAAAAGATGGTGGGCGCGATTGCCGCATACGTTc                                    <  1:1095797/101‑1 (MQ=255)                                                               cGTTTTATAAGCGGCTGAATGCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGATAAAAGATGGTGGGCGCGATTGCCGCATACGTTCAaat                                <  2:238827/101‑1 (MQ=255)                                                                gTTTTATAAGCGGCTGAATGCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGATAAAAGATGGTGGGCGCGATTGCCGCATACGTTCAaata                               >  1:1209762/1‑101 (MQ=255)                                                                gTTTTATAAGCGGCTGAATGCCGGGGGTCGTAAGGGTGGATGCGAGGGGATGCGCTGGTGGGTTAAAGATGGAGGGAGGGATTGCGGCATACATTCAaata                               >  1:1143699/1‑101 (MQ=255)                                                                  tttATAAGCGGCTGAATGCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGATAAAAGATGGTGGGCGCGATTGCCGCATACGTTCAaataac                             <  1:27810/101‑2 (MQ=255)                                                                   ttATAAGCGGCTGAATGCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGATAAAAGATGGTGGGCGCGATTGCCGCATACGTTCAaataact                            <  1:1325795/101‑3 (MQ=255)                                                                    tataAGCGGCTGAATGCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGATAAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTg                           <  2:110419/101‑1 (MQ=255)                                                                         gCGGCTGAATGCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGATAAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTatg                      >  2:892557/1‑99 (MQ=255)                                                                          cGGCTGAATGCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGATAAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATgg                     >  1:1451271/1‑101 (MQ=255)                                                                           ggCTGAATGCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGATAAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATgga                    <  2:1576098/101‑2 (MQ=255)                                                                           ggCTGAATGCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGATAAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATgga                    <  2:1119693/101‑2 (MQ=255)                                                                            gCTGAATGCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGATAAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATggac                   <  1:1702997/101‑3 (MQ=255)                                                                             ctgaaTGCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGATAAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACa                  <  2:1126102/98‑1 (MQ=255)                                                                              tgaaTGCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGATAAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAg                 >  2:963025/3‑101 (MQ=255)                                                                              tgaaTGCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGATAAAAGATGGTGAGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAg                 >  2:11726/3‑101 (MQ=255)                                                                                 aTGCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGATAAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGtt              >  2:311794/1‑101 (MQ=255)                                                                                   gCCGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGATAAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGTTAt            <  2:227805/101‑1 (MQ=255)                                                                                     cGGTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGATAAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGTTATtc          <  2:1145004/101‑1 (MQ=255)                                                                                      ggTGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGATAAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGTTATtcg         <  1:879083/101‑1 (MQ=255)                                                                                        tGATCGTAAGGGTGCATGCGAGGCGATTCGCTGGTGGATAAAAGATGGTGGGCGCGATTGCCGCATACGTTCAAATAACTGCTATGGACAGGTTATtcgtc       >  2:221854/1‑101 (MQ=255)                                                                                             gTAAGGGTGCATGCGAGGCGATTCGCTGGTGGATAAAAGATGGTGGGCGCGATTGCCGCATCCATTCAAATAACTGCTACGGACAGGTTATTCGTCGTGAc  <  2:1143694/101‑1 (MQ=255)                                                                                                 |                                                                                                 GAAAGCCGGTATCGCGTCATTCTGTCCCTATAACATTGGCCCTGGTAAGTGTTTTCCGTCGACGTTTTATAAGCGGCTTAATGCCGGTGATCGTAAAGGCGCATGCGAGGCGATTCGCTGGTGGATAAAGGACGGTGGACGTGATTGCCGCATTCGTTCAAATAATTGTTACGGTCAGGTTATTCGTCGTGAC  >  178/1254‑1446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑