Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	191	1,721	C→T	intergenic (–/–)	– / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	191	1,721	0	C	T	100.0%	150.8 / NA	46	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/17);  total (29/17)

GTTTCGCTGGCTTTGGCATTTGTTTCGCTGGTCTTCGCTGCCGTCTGGCTGGACTTTGCGTTAGTTTCGCTCGTCTTCGCTGCTTTCTGGCTGTTAGCCGCAGCAGTTGCTGATCCAGCTGCTGAAGTCGCAGAACCGGCTGCCGCGCTCTCGCTTTCGGCTGCTGCATTCTGGCTATTTTTCGCCGCAGTTTCA  >  191/1623‑1817                                                                                                   |                                                                                                 gTTTCGCTGGCTTTGGCATTCGTTTCGCTGGTCTTCGCTGCCGTCTGGCTGGACTTTGCGTTAGTTTCGCTCGTCTTCGCTGTTTTCTGGCTGTTAGCTgc                                                                                                >  2:186682/1‑101 (MQ=255) gTTTCGCTGGCTTTGGCATTCGTTTCGCTGGTCTTCGCTGCCGTCTGGCTGGACTTTGCGTTAGTTTCGCTCGTCTTCGCTGCTTTCTGGCTGTTAGCTgc                                                                                                <  2:5962/101‑1 (MQ=255)     cGCTGGCTTTGGCATTCGTTTCGCTGGTCTTCGCTGCCGTCTGGCTGGACTTTGCGTTAGTTTCGCTCGTCTTCGCTGCTTTCTGGCTGTTAGCTgcagca                                                                                            <  1:851897/101‑1 (MQ=255)     cGCTGGCTTTGGCATTCGTTTCGCTGGTCTTCGCTGCCGTCTGGCTGGACTTTGCGTTAGTTTCGCTCGTCTTCGCTGCTTTCTGGCTGTTAGCCgcagca                                                                                            >  2:1031462/1‑101 (MQ=255)      gCTGGCTTTGGCATTCGTTTCGCTGGTCTTCGCTGCCGTCTGGCTGGACTTTGCGTTAGTTTCGCTCGTCTTCGCTGCTTTCTGGCTGTTAGCTGcagcag                                                                                           <  1:745277/101‑1 (MQ=255)       cTGGCTTTGGCATTCGTTTCGCTGGTCTTCGCTGCCGTCTGGCTGGACTTTGCGTTAGTTTCGCTCGTCTTCGCTGCTTTCTGGCTGTTAGCTGCAGCAGt                                                                                          >  2:1636284/1‑101 (MQ=255)        tGGCTTTGGCATTCGTTTCGCTGGTCTTCGCTGCCGTCTGGCTGGACTTTGCGTTAGTTTCGCTCGTCTTCGCTGCTTTCTGGCTGTTAGCTGCAGCAGtt                                                                                         >  1:79316/1‑101 (MQ=255)         ggCTTTGGCATTCGTTTCGCTGGTCTTCGCTGCCGTCTGGCTGGACTTTGCGTTAGTTTCGCTCGTCTTCGCTGCTTTCTGGCTGTTAGCTGCAGCAGTTg                                                                                        <  2:1467809/101‑1 (MQ=255)                  aTTCGTTTCGCTGGTCTTCGCTGCCGTCTGGCTGGACTTTGCGTTAGTTTCGCTCGTCTTCGCTGCTTTCTGGCTGTTAGCTGCAGCAGTTGCTGATCCAg                                                                               >  1:1358388/1‑101 (MQ=255)                  aTTCGTTTCGCTGGTCTTCGCTGCCGTCTGGCTGGACTTTGCGTTAGTTTCGCTCGTCTTCGCTGCTTTCTGGCTGTTAGCTGCAGCAGTTGCTGATCCAg                                                                               >  1:1544546/1‑101 (MQ=255)                            cTGGTCTTCGCTGCCGTCTGGCTGGACGTTGCGTTGGTTTCGCTCGTCTTCGCTGCTTTCTGGCAGTTAGCTGCAGCAGTTGCTGATCCCGCTGCTGAAGt                                                                     >  1:438728/1‑101 (MQ=21)                             tGGTCTTCGCTGCCGTCTGGCTGGACTTTGCGTTAGTTTCGCTCGTCTTCGCTGCTTTCTGGCTGTTAGCTGCAGCAGTTGCTGATCCAGCTGCTGAAGTc                                                                    <  2:1573019/101‑1 (MQ=255)                              ggTCTTCGCTGCCGTCTGGCTGGACTTTGCGTTAGTTTCGCTCGTCTTCGCTGCTTTCTGGCTGTTAGCTGCAGCAGTTGCTGATCCAGCTGCTGAAGTCg                                                                   >  2:520898/1‑101 (MQ=255)                              ggTCTTCGCTGCCGTCTGGCTGGACTTTGCGTTAGTTTCGCTCGTCTTCGCTGCTTTCTGGCTGTTAGCTGCAGCAGTTGCTGATCCAGCTGCTGAAGTCg                                                                   >  2:1323828/1‑101 (MQ=255)                               gTCTTCGCTGCCGTCTGGCTGGACTTTGCGTTAGTTTCGCTCGTCTTCGCTGCTTTCTGGCTGTTAGCTGCAGCAGTTGCTGATCCAGCTGCTGAAGTCGc                                                                  <  1:1636290/101‑1 (MQ=255)                               gTCTTCGCTGCCGTCTGGCTGGACTTTGCGTTAGTTTCGCTCGTCTTCGCTGCTTTCTGGCTGTTAGCTGCAGCAGTTGCTGATCCAGCTGCTGAAGTCGc                                                                  <  2:500764/101‑1 (MQ=255)                                      cTGCCGTCTGGCTGGACTTTGCGTTAGTTTCGCTCGTCTTCGCTGCTTTCTGGCTGTTAGCTGCAGCAGTTGCTGATCCAGCTGCTGAAGTCGCAGAACCg                                                           <  2:673266/101‑1 (MQ=255)                                        gCCGTCTGGCTGTACTTTGCGTTAGTTTCGCTCGTCTTCGCTGCTTTCTGGCTGTTAGCTGCAGCAGTTGCTGATCCAGCTGCTGAAGTCGCAGAACCggn                                                         <  1:789046/101‑2 (MQ=255)                                           gTCTGGCTGGACTTTCCGTTAGTTTCGCTCGTCTTCGCTGCTTTCTGGCTGTTAGCTGCAGCAGTTGCTGATCCAGCTGCTGAAGTCGCAGAACCGGCTGc                                                      >  1:1007739/1‑101 (MQ=255)                                                gctggACTTTGCGTTAGTTTCGCTCGTCTTCGCTGCTTTCTGGCTGTTAGCTGCAGCAGTTGCTGATCCAGCTGCTGAAGTCGCAGAACCGGCTGCcgcac                                                 >  2:1665854/1‑99 (MQ=255)                                                 ctggACTTTGCGTTAGTTTCGCTCGTCTTCGCTGCTTTCTGGCTGTTAGCTGCAGCAGTTGCTGATCCAGCTGCTGAAGTCGCAGAACCGGCTGCCGCAct                                                >  2:1230394/1‑101 (MQ=255)                                                     aCTTTGCGTTAGTTTCGCTCGTCTTCGCTGCTTTCTGGCTGTTAGCTGCAGCAGTTGCTGATCCAGCTGCTGAAGTCGCAGAACCGGCTGCCGCACTCTCg                                            <  2:79316/101‑1 (MQ=255)                                                       tttGCGTTAGTTTCGCTCGTCTTCGCTGCTTTCTGGCTGTTAGCTGCAGCAGTTGCTGATCCAGCTGCTGAAGTCGCAGAACCGGCTGCCGCACTCTCGCt                                          >  2:1457439/1‑101 (MQ=255)                                                         tGCGTTAGTTTCGCTCGTCTTCGCTGCTTTCTGGCTGTTAGCTGCAGCAGTTGCTGATCCAGCTGCTGAAGTCGCAGAACCGGCTGCCGCACTCTCGCttt                                        >  1:1089042/1‑101 (MQ=255)                                                           cGTTAGTTTCGCTCGTCTTCGCTGCTTTCTGGCTGTTAGCTGCAGCAGTTGCTGATCCAGCTGCTGAAGTCGCAGAACCGGCTGCCGCACTCTCGCTTTCg                                      <  2:414604/101‑1 (MQ=255)                                                           cGTTAGTTTCGCTCGTCTTCGCTGCTTTCTGGCTGTTAGCTGCAGCAGTTGCTGATCCAGCTGCTGAAGTCGCAGAACCGGCTGCCGCACTCTCGCTTTCg                                      >  2:1542394/1‑101 (MQ=255)                                                            gTTAGTTTCGCTCGTCTTCGCTGCTTTCTGGCTGTTAGCTGCAGCAGTTGCTGATCCAGCTGCTGAAGTCGCAGAACCGGCTGCCGCACTCTCGCTTTCgg                                     >  1:787737/1‑101 (MQ=255)                                                            gTTAGTTTCGCTCGTCTTCGCTGCTTTCTGGCTGTTAGCTGCAGCAGTTGCTGATCCAGCTGCTGAAGTCGCAGAACCGGCTGCCGCACTCTCGCTTTCgg                                     >  2:1430735/1‑101 (MQ=255)                                                              tAGTTTCGCTCGTCTTCGCTGTTTTCTGGCTGTTAGCTGCAGCAGTTGCTGATCCAGCTGCTGAAGTCGCAGAACCGGCTGCCGCACTCTCGCTTTCGgct                                   <  1:186682/101‑1 (MQ=255)                                                              tAGTTTCGCTCGTCTTCGCTGCTTTCTGGCTGTTAGCTGCAGCAGTTGCTGATCCAGCTGCTGAAGTCGCAGAACCGGCTGCCGCACTCTCGCTTTCGgct                                   >  1:252561/1‑101 (MQ=255)                                                              tAGTTTCGCTCGTCTTCGCTGCTTTCTGGCTGTTAGCTGCAGCAGTTGCTGATCCAGCTGCTGAAGTCGCAGAACCGGCTGCCGCACTCTCGCTTTCGgct                                   <  2:897057/101‑1 (MQ=255)                                                                 tttCGCTCGTCTTCGCTGCTTTCTGGCTGTTAGCTGCAGCAGTTGCTGATCCAGCTGCTGAAGTCGCAGAACCGGCTGCCGCACTCTCGCTTTCGgctgct                                <  1:940563/101‑1 (MQ=255)                                                                   tCGCTCGTCTTCGCTGCTTTCTGGCTGTTAGCTGCAGCAGTTGCTGATCCAGCTGCTGAAGTCGCAGAACCGGCTGCCGCACTCTCGCTTTCGGCtgctgc                              >  2:1181679/1‑101 (MQ=255)                                                                     tcTCGTCTTCGCTGCTTTCTGGCTGTTAGCTGCAGCAGTTGCTGATCCAGCTGCTGAAGTCGCAGAACCGGCTGCCGCACTCTCGCTTTCGGCTGCTGCan                            <  1:499190/100‑2 (MQ=255)                                                                     gCTCGTCTTCGCTGCTTTCTGGCTGTTAGCTGCAGCAGTTGCTGATCCAGCTGCTGAAGTCGCAGAACCGGCTGCCGCACTCTCGCTTTCGGCTGCTGCag                            <  1:439117/101‑2 (MQ=255)                                                                       tCGTCTTCGCTGCTTTCTGGCTGTTAGCTGCAGCAGTTGCTGATCCAGCTGCTGAAGTCGCAGAACCGGCTGCCGCACTCTCGCTTTCGGCTGCTGCagcc                          >  1:485582/1‑98 (MQ=255)                                                                        cGTCTTCGCTGCTTTCTGGCTGTTAGCTGCAGCAGTTGCTGATCCAGCTGCTGAAGTCGCAGAACCGGCTGCCGCACTCTCGCTTTCGGCTGCTGCagcct                         >  2:55742/1‑97 (MQ=38)                                                                         gTCTTCGCTGCTTTCTGGCTGTTAGCTGCAGCAGTTGCTGATCCAGCTGCTGAAGTCGCAGAACCGGCTGCCGCACTCTCGCTTTCGGCTGCTGCagcctg                        >  1:1322637/1‑96 (MQ=38)                                                                           cTTCGCTGCTTTCTGGCTGTTAGCTGCAGCAGTTGCTGATCCAGCTGCTGAAGTCGCAGAACCGGCTGCCGCACTCTCGCTTTCGGCTGCTGCagcctgac                      >  2:320482/1‑94 (MQ=37)                                                                            ttCGCTGCTTTCTGGCTGTTAGCTGCAGCAGTTGCTGTTCCAGCTGCTGAAGTCGCAGAACCGGCTGCCGCACTCTCGCTTTCTGCTGCTGCagcctgact                     >  2:446111/1‑93 (MQ=255)                                                                                      tCTGGCTGTTAGCTGCAGCAGTTGCTGATCCAGCTGCTGAAGTCGCAGAACCGGCTGCCGCACTCTCGCTTTCGGCTGCTGCAGCCTGACTGCTTTTcgcc           >  1:982845/1‑101 (MQ=21)                                                                                        tGGCTGTTAGCTGCAGCAGTTGCTGATCCAGCTGCTGAAGTCGCAGAACCGGCTGCCGCACTCTCGCTTTCGGCTGCTGCAGCCTGACTGCTTTTcgccgc         >  2:364344/1‑101 (MQ=18)                                                                                        tGGCTGTTAGCTGCAGCAGTTGCTGATCCAGCTGCTGAAGTCGCAGAACCGGCTGCCGCACTCTCGCTTTCGGCTGCTGCAGCCTGACTGCTTTTcgccgc         >  2:913244/1‑101 (MQ=21)                                                                                        tGGCTGTTAGCTGCAGCAGTTGCTGATCCAGCTGCTGAAGTCGCAGAACCGGCTGCCGCACTCTCGCTTTCGGCTGCTGCAGCCTGACTGCTTTTcgccgc         >  1:800298/1‑101 (MQ=18)                                                                                           cTGTTAGCTGCAGCAGTTGCTGATCCAGCTGCTGAAGTCGCAGAACCGGCTGCCGCACTCTCGCTTTCGGCTGCTGCAGCCTGACTGCTTTTCGCCGCAGt      <  2:66010/101‑1 (MQ=18)                                                                                            tGTTAGCTGCAGCAGTTGCTGATCCAGCTGCTGAAGTCGCAGAACCGGCTGCCGCACTCTCGCTTTCGGCTGCTGCAGCCTGACTGCCTTTCGCCGCAGtt     >  1:705517/1‑101 (MQ=18)                                                                                             gTTAGCTGCAGCAGTTGCTGATCCAGCTGCTGAAGTCGCAGAACCGGCTGCCGCACTCTCGCTTTCGGCTGCTGCAGCCTGACTGCTTTTCGCCGCAGgtt    >  2:311560/1‑101 (MQ=18)                                                                                               tAGCTGCAGCAGTTGCTGATCCAGCTGCTGAAGTCGCAGAACCGGCTGCCGCACTCTCGCTTTCGGCTGCTGCAGCCTGACTGATTTTCGCCGCAGTTTCa  >  1:343351/1‑101 (MQ=18)                                                                                                   |                                                                                                 GTTTCGCTGGCTTTGGCATTTGTTTCGCTGGTCTTCGCTGCCGTCTGGCTGGACTTTGCGTTAGTTTCGCTCGTCTTCGCTGCTTTCTGGCTGTTAGCCGCAGCAGTTGCTGATCCAGCTGCTGAAGTCGCAGAACCGGCTGCCGCGCTCTCGCTTTCGGCTGCTGCATTCTGGCTATTTTTCGCCGCAGTTTCA  >  191/1623‑1817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑