Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	106	9,337	G→A	intergenic (–/–)	– / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	106	9,337	0	G	A	100.0%	161.2 / NA	47	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/23);  total (24/23)

ATTCTGCTTCTGGCAGGGGAATTAATCGGTCGCGGCGTATTCTATGGTTTGCATATGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGGGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTAACGTGTCATAACTGGTCATCAAATTACGATAATCAGGAATGTGGTTAGAGAACAACGTC  >  106/9241‑9434                                                                                                 |                                                                                                  attcTGCTTCTGGCAGGGGAATTAATCGGTCGCGGCGTATTCTATGGTTTGCATATGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGtt                                                                                               <  2:856254/101‑1 (MQ=255)  ttcTGCTTCTGGCAGGGGAATTAATCGGTCGCGGCGTATTCTATGGTTTGCATATGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGttt                                                                                              >  2:1122881/1‑101 (MQ=255)    cTGCTTCTGGCAGGGGAATTAATCGGTCGCGGCGTATTCTATGGTTTGCATATGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCg                                                                                            >  1:738691/1‑101 (MQ=255)    cTGCTTCTGGCAGGGGAATTAATCGGTCGCGGCGTATTCTATGGTTTGCATATGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCg                                                                                            <  1:402148/101‑1 (MQ=255)       cTTCTGGCAGGGGAATTAATCGGTCGCGGCGTATTCTATGGTTTGCATATGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGcc                                                                                         >  1:1235662/1‑101 (MQ=255)              cAGGGGAATTAATCGGTCGCGGCGTATTCTATGGTTTGCATATGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTtc                                                                                  >  1:934367/1‑101 (MQ=255)               aGGGGAATTAATCGGTCGCGGCGTATTCTATGGTTTGCATATGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTtct                                                                                 >  2:1678403/1‑101 (MQ=255)               aGGGGAATTAATCGGTCGCGGCGTATTCTATGGTTTGCATATGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTtct                                                                                 >  2:1478280/1‑101 (MQ=255)                ggggAATTAATCGGTCGCGGCGTATTCTATGGTTTGCATATGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTtctc                                                                                >  1:1668568/1‑101 (MQ=255)                     aTTAATCGGTCGCGGCGTATTCTATGGTTTGCATATGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTa                                                                           >  1:1298003/1‑101 (MQ=255)                     aTTAATCGGTCGCGGCGTATTCTATGGTTTGCATATGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTa                                                                           >  1:860848/1‑101 (MQ=255)                          tCGGTCGCGGCGTATTCTATGGTTTGCATATGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCt                                                                      <  2:1402577/101‑1 (MQ=255)                          tCGGTCGCGGCGTATTCTATGGTTTGCATATGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCt                                                                      <  2:812307/101‑1 (MQ=255)                            ggTCGCGGCGTATTCTATGGTTTGCATATGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGc                                                                    >  1:1633947/1‑101 (MQ=255)                             gTCGCGGCGTATTCTATGGTTTGCATATGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCt                                                                   <  2:584165/101‑1 (MQ=255)                              tCGCGGCGTATTCTATGGTTTGCATATGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCtt                                                                  >  2:1702691/1‑101 (MQ=255)                                  ggcgTATTCTATGGTTTGCATATGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGtt                                                              >  1:662485/1‑101 (MQ=255)                                    cgTATTCTATGGTTTGCATATGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTaa                                                            >  2:335789/1‑101 (MQ=255)                                     gTATTCTATGGTTTGCATATGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTAAc                                                           >  2:1590651/1‑101 (MQ=255)                                     gTATTCTATGGTTTGCATATGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTAAc                                                           <  2:562066/101‑1 (MQ=255)                                     gTATTCTATGGTTTGCATATGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTAAc                                                           >  1:47331/1‑101 (MQ=255)                                       aTTCTATGGTTTGCATATGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTAACgt                                                         >  1:216165/1‑101 (MQ=255)                                       aTTCTATGGTTTGCATATGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTAACgt                                                         >  1:1631565/1‑101 (MQ=255)                                         tCTATGGTTTGCATATGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTAACgtgt                                                       <  2:47331/101‑1 (MQ=255)                                         tCTATGGTTTGCATATGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTAACgtgt                                                       <  1:1152499/101‑1 (MQ=255)                                          cTATGGTTTGCATATGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTAACGTGTc                                                      <  2:1178682/101‑1 (MQ=255)                                              ggTTTGCATATGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTAACGTGTCATaa                                                  >  2:158039/1‑101 (MQ=255)                                                      tatGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTAACGTGTCATAACTGGtcat                                          >  1:129692/1‑101 (MQ=255)                                                       atGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTAACGTGTCATAACTGGtcatc                                         >  1:1021684/1‑101 (MQ=255)                                                         gACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTAACGTGTCATAACTGGTCATCaa                                       <  1:1562076/101‑1 (MQ=255)                                                          aCCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTAACGTGTCATAACTGGTCATCaaa                                      <  2:1474635/101‑1 (MQ=255)                                                          aCCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTAACGTGTCATAACTGGTCATCaaa                                      <  1:1705900/101‑1 (MQ=255)                                                           ccGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTAACGTGTCATAACTGGTCATCAAAt                                     <  1:1678409/101‑1 (MQ=255)                                                             gTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTAACGTGTCATAACTGGTCATCAAATTa                                   <  2:415798/101‑1 (MQ=255)                                                             gTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTAACGTGTCATAACTGGTCATCAAATTa                                   >  1:790660/1‑101 (MQ=255)                                                              tGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTAACGTGTCATAACTGGTCATCAAATTAc                                  <  2:1416204/101‑1 (MQ=255)                                                                gggCTGGCCGTCTCAAGCTAATCATAACAACCGAGGTTTCGGCGCCGGTTCTCTTTTATTTCTGCTTCGTTAACGTGTCATAACTGGTCATCAAATTACGa                                <  1:516686/101‑1 (MQ=255)                                                                 ggATGGCCGGCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTAACGTGTCATAACTGGTCATCAAATTACGAt                               >  1:1234053/1‑101 (MQ=255)                                                                  gATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTAACGTGTCATAACTGGTCATCAAATTACGATa                              <  2:1021679/101‑1 (MQ=255)                                                                   aTGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTAACGTGTCATAACTGGTCATCAAATTACGATaa                             >  2:1440327/1‑101 (MQ=255)                                                                   aTGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTAACGTGTCATAACTGGTCATCAAATTACGATaa                             <  2:662480/101‑1 (MQ=255)                                                                      gCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTAACGTGTCATAACTGGTCATCAAATTACGATAATCa                          <  1:1122886/101‑1 (MQ=255)                                                                             cAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTAACGTGTCATAACTGGTCATCAAATTACGATAATCAGGAAtgt                   <  2:180911/101‑1 (MQ=255)                                                                               aGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTAACGTGTCATAACTGGTCATCAAATTACGATAATCAGGAATGTgg                 <  2:1297997/101‑1 (MQ=255)                                                                                      cATAACAACCGAGGTTTCGTCCCCGGTTCTCTTTTATTTCTGCTTCGTTAACGTGTCATAACTGGTAATCAAATTACGATAATCAGGAATGTGGTTAgaga          >  2:1640148/1‑101 (MQ=255)                                                                                       aTAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTAACGTGTCATAACTGGTCATCAAATTACGATAATCAGGAATGTGGTTAGAGaa         >  2:33732/1‑101 (MQ=255)                                                                                       aTAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTAACGTGTCATAACTGGTCATCAAATTACGATAATCAGGAATGTGGTTAGAGaa         <  2:23346/101‑1 (MQ=255)                                                                                           caacCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTAACGTGTCATAACTGGTCATCAAATTACGATAATCAGGAATGTGGTTAGAGaacaac     <  2:943127/101‑1 (MQ=255)                                                                                            aacCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTAACGTGTCATAACTGGTCATCAAATTACGATAATCAGGAATGTGGTTAGAGAACAACg    <  1:1211231/101‑1 (MQ=255)                                                                                              ccGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTAACGTGTCATAACTGGTCATCAAATTACGATAATCAGGAATGTGGTTAGAGAACAACGTc  <  2:44001/101‑1 (MQ=255)                                                                                                 |                                                                                                  ATTCTGCTTCTGGCAGGGGAATTAATCGGTCGCGGCGTATTCTATGGTTTGCATATGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGGGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTAACGTGTCATAACTGGTCATCAAATTACGATAATCAGGAATGTGGTTAGAGAACAACGTC  >  106/9241‑9434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑