Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	10	47,083	G→A	intergenic (–/–)	– / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	10	47,083	0	G	A	100.0%	231.6 / NA	66	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/42);  total (24/42)

GGGCGAAATCGTCGCCGCGTTGCAGGAGCAAGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACGCAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTGAACTGGGTGTACCAACCACCTCCAGTCCATTGAAAAATCTGGTGCTGGATATCGACT  >  10/46985‑47178                                                                                                   |                                                                                                gggCGAAATCGTCGCCGCGTTGCAGGAGCAAGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTTACCAAGTTTTGTGGTTTACGTATAcaca                                                                                               >  2:1304412/1‑101 (MQ=255)   gCGAAATCGTCGCCGCGTTGCAGGAGCAAGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAAt                                                                                             <  1:1080650/101‑1 (MQ=255)   gCGAAATCGTCGCCGCGTTGCAGGAGCAAGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAAt                                                                                             <  1:15000/101‑1 (MQ=255)    cGAAATCGTCGCCGCGTTGCAGGAGCAAGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATg                                                                                            <  1:1575684/101‑1 (MQ=255)    cGAAATCGTCGCCGCGTTGCAGGAGCAAGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATg                                                                                            <  2:866631/101‑1 (MQ=255)     gAAATCGTCGCCGCGTTGCAGGAGCAAGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTGCGTACACACAATGc                                                                                           <  2:627295/101‑1 (MQ=255)     gAAATCGTCGCCGCGTTGCAGGAGCAAGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGc                                                                                           >  2:956916/1‑101 (MQ=255)      aaaTCGTCGCCGCGTTGCAGGAGCAAGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGcc                                                                                          >  2:812845/1‑101 (MQ=255)        aTCGTCGCCGCGTTGCAGGAGCAAGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTTGTGCTGTACGTACACACAATGCCaa                                                                                        >  2:503016/1‑101 (MQ=255)         tcgtcgCCGCGTTGCAGGAGCAAGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCaaa                                                                                       <  1:1019995/101‑1 (MQ=255)           gtcgCCGCGTTGCAGGAGCAAGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAaaat                                                                                     >  2:1431959/1‑101 (MQ=255)           gtcgCCGCGTTGCAGGAGCAAGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAaaat                                                                                     <  1:503802/101‑1 (MQ=255)           gtcgCCGCGTTGCAGGAGCAAGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAaaat                                                                                     <  1:902233/101‑1 (MQ=255)                 gcgTTGCAGGAGCAAGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAaaatggaaat                                                                               <  1:126063/101‑1 (MQ=255)                   gTTGCAGGAGCAAGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCTAaaatggaaatgg                                                                             <  2:277258/101‑1 (MQ=255)                   gTTGCAGGAGCAAGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAaaatggaaatgg                                                                             <  2:516163/101‑1 (MQ=255)                   gTTGCAGGAGCAAGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAaaatggaaatgg                                                                             <  1:1396843/101‑1 (MQ=255)                      gCAGGAGCAAGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGttt                                                                          <  1:437910/101‑1 (MQ=255)                       cAGGAGCAAGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTa                                                                         >  2:325009/1‑101 (MQ=255)                        aGGAGCAAGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTAc                                                                        <  2:966073/101‑1 (MQ=255)                        aGGAGCAAGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTAc                                                                        <  1:843978/101‑1 (MQ=255)                        aGGAGCAAGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTAc                                                                        <  1:594919/101‑1 (MQ=255)                        aGGAGCAAGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTAc                                                                        >  1:729725/1‑101 (MQ=255)                         ggAGCAAGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTAct                                                                       <  1:93420/101‑1 (MQ=255)                         ggAGCAAGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTAct                                                                       >  1:451941/1‑101 (MQ=255)                           aGCAAGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTActgc                                                                     <  2:335354/101‑1 (MQ=255)                            gCAAGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTActgcc                                                                    <  1:1402181/101‑1 (MQ=255)                             cAAGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTActgcct                                                                   >  2:915468/1‑101 (MQ=255)                             cAAGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTActgcct                                                                   >  2:1674047/1‑101 (MQ=255)                              aaGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTActgcctg                                                                  <  2:998758/101‑1 (MQ=255)                              aaGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTActgcctg                                                                  >  1:996453/1‑101 (MQ=255)                               aGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTActgcctgc                                                                 <  2:951853/101‑1 (MQ=255)                                ggCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCt                                                                >  1:606843/1‑101 (MQ=255)                                 gCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTa                                                               >  1:1522138/1‑101 (MQ=255)                                 gCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTa                                                               <  2:17809/101‑1 (MQ=255)                                  cTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTAg                                                              >  2:357347/1‑101 (MQ=255)                                   tttGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGc                                                             >  2:9474/1‑101 (MQ=255)                                    ttGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCt                                                            <  2:1146444/101‑1 (MQ=255)                                     tGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTg                                                           <  2:469080/101‑1 (MQ=255)                                     tGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTg                                                           >  1:1567096/1‑101 (MQ=255)                                      gACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTGa                                                          >  1:347257/1‑101 (MQ=255)                                            atTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTGAACTggg                                                    <  2:1685251/101‑1 (MQ=255)                                             ttAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTGAACTGGgt                                                   >  1:78753/1‑101 (MQ=255)                                               aaTCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTGAACTGGgtgt                                                 <  1:1281043/101‑1 (MQ=255)                                               aaTCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTGAACTGGgtgt                                                 >  2:269734/1‑101 (MQ=255)                                                   aGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTGAACTGGGTGTacca                                             <  2:1222400/101‑1 (MQ=255)                                                    gTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTGAACTGGGTGTaccan                                            <  1:668863/101‑2 (MQ=255)                                                    gTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTGAACTGGGTGTaccaa                                            >  2:903487/1‑101 (MQ=255)                                                     tCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTGAACTGGGTGTaccaac                                           >  1:1259738/1‑101 (MQ=255)                                                      cTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTGAACTGGGTGTaccaacc                                          <  1:807047/101‑1 (MQ=255)                                                      cTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTGAACTGGGTGTaccaacc                                          <  2:1483100/101‑1 (MQ=255)                                                      cTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTGAACTGGGTGTaccaacc                                          >  2:691419/1‑101 (MQ=255)                                                       tAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTGAACTGGGTGGaccaacca                                         >  2:997436/1‑101 (MQ=255)                                                        aaaGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTGAACTGGGTGTACCAaccac                                        >  1:523675/1‑101 (MQ=255)                                                          aGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTGAACTGGGTGTACCAACCACCt                                      >  2:498772/1‑101 (MQ=255)                                                          aGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTGAACTGGGTGTACCAACCACCt                                      <  2:1262429/101‑1 (MQ=255)                                                          aGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTGAACTGGGTGTACCAACCACCt                                      <  1:876903/101‑1 (MQ=255)                                                              tcGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTGAACTGGGTGTACCAACCACCTCCAg                                  <  2:1569125/101‑1 (MQ=255)                                                              tcGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTGAACTGGGTGTACCAACCACCTCCAg                                  <  2:423457/101‑1 (MQ=255)                                                                     tGTTGACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTGAACTGGGTGTACCAACCACCTCCAGTCCATTg                           <  1:632266/101‑1 (MQ=255)                                                                         gACCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTGAACTGGGTGTACCAACCACCTCCAGTCCATTGaaaa                       <  1:20812/101‑1 (MQ=255)                                                                          aCCAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTGAACTGGGTGTACCAACCACCTCCAGTCCATTGaaaaa                      <  1:956921/101‑1 (MQ=255)                                                                           ccAAGTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTGAACTGGGTGTACCAACCACCTCCAGTCCATTGAAAAAt                     <  2:606838/101‑1 (MQ=255)                                                                               gTTTGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTGAACTGGGTGTACCAACCACCTCCAGTCCATTGAAAAATCTgg                 <  1:357347/101‑1 (MQ=255)                                                                                tttGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTGAACTGGGTGTACCAACCACCTCCAGTCCATTGAAAAATCTGGt                <  2:864023/101‑1 (MQ=255)                                                                                tttGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTGAACTGGGTGTACCAACCACCTCCAGTCCATTGAAAAATCTGGt                <  1:1258535/101‑1 (MQ=255)                                                                                 ttGGTGCTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTGAACTGGGTGTACCAACCACCTCCAGTCCATTGTAAAATCTGGTg               <  2:1317073/101‑1 (MQ=255)                                                                                       cTGTACGTACACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTGAACTGGGTGTACCAACCACCTCCAGTCCATTGAAAAATCTGGTGCTGGat         >  1:1324933/1‑101 (MQ=255)                                                                                         gtacgtacACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTGAACTGGGTGTACCAACCACCTCCAGTCCATTGAAAAATCTGGTGCTGGatat       <  1:1130695/101‑1 (MQ=255)                                                                                         gtacgtacACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTGAACTGGGTGTACCAACCACCTCCAGTCCATTGAAAAATCTGGTGCTGGatat       <  1:1015810/101‑1 (MQ=255)                                                                                              tacACACAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTGAACTGGGTGTACCAACCACCTCCAGTCCATTGAAAAATCTGGTGCTGGATATCGACt  >  2:1542402/1‑101 (MQ=255)                                                                                                   |                                                                                                GGGCGAAATCGTCGCCGCGTTGCAGGAGCAAGGCTTTGACAATATTAATCAGTCTAAAGTCTCGCGGATGTTGACCAAGTTTGGTGCTGTACGTACACGCAATGCCAAAATGGAAATGGTTTACTGCCTGCTAGCTGAACTGGGTGTACCAACCACCTCCAGTCCATTGAAAAATCTGGTGCTGGATATCGACT  >  10/46985‑47178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑