Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*312,0730CT58.0% 51.1 / 117.3 100intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (27/15);  new base T (36/22);  total (63/37)
Fisher's exact test for biased strand distribution p-value = 8.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.34e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 112 total aligned reads displayed.

ATCAGCAAATCAATCTGCGTGACGCTTACGTTGATCACCTGTTCGGTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATCAACTCCGGGAACGGCGCAGCGGGTCCGGTGGTGGACGCTATCGAAGCCCGCTTTAACGCCCTCGGCGCTCCGGTGGAATTAATCAAAGTGCACAACACGCCGGACGGCAATT  >  31/1973‑2173
                                                                                                    |                                                                                                    
aTCAGCAAATCAATCTGCGTGACGCTTACGTTGATCACCTGTTCGGTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATCAACTCCGGGAAc                                                                                                      >  1:935930/1‑101 (MQ=255)
aTCAGCAAATCAATCTGCGCTACGCCTACATTGACCACCTGTGCGGTTATATAAACGCCAAAAAACTCACGCCGCTCAAGCTGGTCACAAACTCCGGGAAc                                                                                                      >  2:981806/1‑101 (MQ=255)
 tCAGCAAATCAATCTGCGTGACGCTTACGTTGATCACCTGTTCGGTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATCAACTCCGGGAACg                                                                                                     >  2:212934/1‑101 (MQ=255)
  cAGCAAATCAATCTGCGTGACGCTTACGTTGATCACCTGTTCGGTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATTAACTCCGGGAATgg                                                                                                    <  2:934577/101‑1 (MQ=255)
   aGCAAATCAATCTGCGTGACGCTTACGTTGATCACCTGTTCGGTTATCTCAACGTTAAAAACCTCACGCCGCTCAAGCTGGCGATCAACTCCGGGAACGgc                                                                                                   >  2:391721/1‑101 (MQ=255)
   aGCAAATCAATCTGCGTGACGCTTACGTTGATCACCTGTTCGGTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATTAACTCCGGGAATGgc                                                                                                   >  1:784421/1‑101 (MQ=255)
   aGCAAATCAATCTGCGTGACGCTTACGTTGATCACCTGTTCGGTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATCAACTCCGGGAACGgc                                                                                                   >  2:1293473/1‑101 (MQ=255)
   aGCAAATCAATCTGCGTGACGCTTACGTTGATCACCTGTTCGGTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATCAACTCCGGGAACGgc                                                                                                   <  1:507047/101‑1 (MQ=255)
     cAAATCAATCTGCGTGACGCTTACGTTGATCACCTGTTCGGTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATTAACTCCGGGAATGgcgc                                                                                                 >  1:206764/1‑101 (MQ=255)
      aaaTCAATCTGCGTGACGCTTACGTTGATCACCTGTTCGGTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATTAACTCCGGGAATGgcgcg                                                                                                >  1:746629/1‑100 (MQ=255)
      aaaTCAATCTGCGTGACGCTTACGTTGATCACCTGTTCGGTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATCAACTCCGGGAACGGCGCa                                                                                                <  1:1176643/101‑1 (MQ=255)
      aaaTCAATCTGCGTGACGCTTACGTTGATCACCTGTTCGGTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATCAACTCCGGGAACGGCGCa                                                                                                >  2:678062/1‑101 (MQ=255)
        atcaatcTGCGTGACGCTTACGTTGATCACCTGTTCGGTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATTAACTCCGGGAATGGCGCGGc                                                                                              >  2:299191/1‑101 (MQ=255)
         tcaatcTGCGTGACGCTTACGTTGATCACCTGTTCGGTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATTAACTCCGGGAATGGCgcggcg                                                                                             <  1:494183/101‑1 (MQ=255)
           aatcTGCGTGACGCTTACGTTGATCACCTGTTCGGTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATCAACTCCGGGAACGGCGCAGCggg                                                                                           >  2:802852/1‑101 (MQ=255)
             tcTGCGTGACGCTTACGTTGATCACCTGTTCGGTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATCAACTCCGGGAACGGCGCAGCGGGTc                                                                                         <  2:1247395/101‑1 (MQ=255)
              cTGCGTGACGCTTACGTTGATCACCTGTTCGGTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATTAACTCCGGGAATGGCGCGGCGGGGcc                                                                                        >  1:568191/1‑101 (MQ=255)
              cTGCGTGACGCTTACGTTGATCACCTGTTCGGTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATTAACTCCGGGAATGGCGCGGCGGGGcc                                                                                        <  2:786186/101‑1 (MQ=255)
               tGCGTGACGCTTACGTTGATCACCTGTTCGGTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATTAACTCCGGGAATGGCGCGGCGGGGCCg                                                                                       <  2:743112/101‑1 (MQ=255)
               tGCGTGACGCTTACGTTGATCACCTGTTCGGTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATTAACTCCGGGAATGGCGCGGCGGGGCCg                                                                                       <  2:1192800/101‑1 (MQ=255)
                gCGTGACGCTTACGTTGATCACCTGTTCGGTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATTAACTCCGGGAATGGCGCGGCGGGGCCgg                                                                                      <  2:796299/101‑1 (MQ=255)
                 cGTGACGCTTACGTTGATCACCTGTTCGGTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATCAACTCCGGGAACGGCGCAGCGGGTCCggg                                                                                     >  2:682958/1‑100 (MQ=255)
                   tGACGCTTACGTTGATCACCTGTTCGGTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATTAACTCCGGGAATGGCGCGGCGGGGCCggtgg                                                                                   >  1:1538693/1‑101 (MQ=255)
                   tGACGCTTACGTTGATCACCTGTTCGGTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATTAACTCCGGGAATGGCGCGGCGGGGCCggtgg                                                                                   >  2:48569/1‑101 (MQ=255)
                   tGACGCTTACGTTGATCACCTGTTCGGTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATCAACTCCGGGAACGGCGCACCGGGTCCggtgg                                                                                   >  2:944200/1‑101 (MQ=255)
                       gCTTACGTTGATCACCTGTTCGGTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATTAACTCCGGGAATGGCGCGGCGGGGCCGGTGGTGGa                                                                               >  1:1678073/1‑101 (MQ=255)
                       gCTTACGTTGATCACCTGTTCGGTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATTAACTCCGGGAATGGCGCGGCGGGGCCGGGGGTGGa                                                                               >  2:1439730/1‑101 (MQ=255)
                        cTTACGTTGATCACCTGTTCGGTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATCAACTCCGGGAACGGCGCAGCGGGTCCGGTGGTGGAc                                                                              <  1:212934/101‑1 (MQ=255)
                           aCGTTGATCACCTGTTCGGTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATCAACTCCGGGAACGGCGCAGCGGGTCCGGTGGTGGACGCt                                                                           >  2:73858/1‑101 (MQ=255)
                            cGTTGATCACCTGTTCGGTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATCAACTCCGGGAACGGCGCAGCGGGTCCGGTGGTGGACGCTa                                                                          >  2:1557487/1‑101 (MQ=255)
                             gTTGATCACCTGTTCGGTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATTAACTCCGGGAATGGCGCGGCGGGGCCGGTGGTGGACGCCAt                                                                         >  1:796201/1‑101 (MQ=255)
                                 aTCACCTGTTCGGTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATTAACTCCGGGAATGGCGCGGCGGGGCCGGTGGTGGACGCCATTGaa                                                                     <  1:1703355/101‑1 (MQ=255)
                                  tCACCTGTTCGGTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATTAACTCCGGGAATGGCGCGGCGGGGCCGGTGGTGGACGCCATTGAAg                                                                    <  1:1548914/101‑1 (MQ=255)
                                  tCACCTGTTCGGTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATTAACTCCGGGAATGGCGCGGCGGGGCCGGTGGTGGACGCCATTGAAg                                                                    >  2:1134486/1‑101 (MQ=255)
                                   cACCTGTTCGGTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATCAACTCCGGGAACGGCGCAGCGGGTCCGGTGGTGGACGCTATCGAAGc                                                                   >  1:1642359/1‑101 (MQ=255)
                                    aCCTGTTCGGTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATTAACTCCGGGAATGGCGCGGCGGGGCCGGTGGTGGACGCCATTGAAGcc                                                                  >  2:162301/1‑101 (MQ=255)
                                    aCCTGTTCGGTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATCAACTCCGGGAACGGCGCAGCGGGTCCGGTGGTGGACGCTATCGAAGcc                                                                  >  1:478968/1‑101 (MQ=255)
                                    aCCTGTTCGGTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATCAACTCCGGGAACGGCGCAGCGGGTCCGGTGGTGGACGCTATCGAAGcc                                                                  >  1:1638643/1‑101 (MQ=255)
                                     ccTGTTCGGTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATTAACTCCGGGAATGGCGCGGCGGGGCCGGTGGTGGACGCCATTGAAGccc                                                                 >  1:1546573/1‑101 (MQ=255)
                                      cTGTTCGGTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATTAACTCCGGGAATGGCGCGGCGGGGCCGGTGGTGGACGCCATTGAAGCCCg                                                                >  1:949184/1‑101 (MQ=255)
                                      cTGTTCGGTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATCAACTCCGGGAACGGCGCAGCGGGTCCGGTGGTGGACGCTATCGAAGCCCg                                                                <  2:850131/101‑1 (MQ=255)
                                       tGTTCGGTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATCAACTCCGGGAACGGCGCAGCGGGTCCGGTGGTGGACGCTATCGAAGCCCGc                                                               >  2:1337651/1‑101 (MQ=255)
                                        gTTCGGTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATTAACTCCGGGAATGGCGCGGCGGGGCCGGTGGTGGACGCCATTGAAGCCCGCt                                                              >  1:1121732/1‑101 (MQ=255)
                                        gTTCGGTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATTAACTCCGGGAATGGCGCGGCGGGGCCGGTGGTGGACGCCATTGAAGCCCGCt                                                              >  1:473139/1‑101 (MQ=255)
                                          tCGGTTATATCAACGTCAAAAACCTCACGCTGCTCAAGCTGGTGATCAACTCCGGGAACGGCGCAGCGGGTCCGGTGGTGGACGCTATCGAAGCCCGCttt                                                            <  1:416266/101‑1 (MQ=255)
                                            ggTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATTAACTCCGGGAATGGCGCGGCGGGGCCGGTGGTGGACGCCATTGAAGCCCGCTTTaa                                                          <  1:214504/101‑1 (MQ=255)
                                            ggTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATTAACTCCGGGAATGGCGCGGCGGGGCCGGTGGTGGACGCCATTGAAGCCCGCTTTaa                                                          >  1:1556150/1‑101 (MQ=255)
                                             gTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATTAACTCCGGGAATGGCGCGGCGGGGCCGGTGGTGGACGCCATTGAAGCCCGCTTTaaa                                                         >  2:865760/1‑100 (MQ=255)
                                             gTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATTAACTCCGGGAATGGCGCGGCGGGGCCGGTGGTGGACGCCATTGAAGCCCGCTTTaaa                                                         <  1:1168092/101‑2 (MQ=255)
                                              ttATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGGGCTTAACTCCGGGAATGGCGCGGCGGGGCCGGCGGTGGACGCCATTGAAGCCCGATTTaaac                                                        >  2:803436/1‑99 (MQ=255)
                                               tataTCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATCAACTCCGGGAACGGCGCAGCGGGTCCGGTGGTGGACGCTATCGAAGCCCGCTTTAACGc                                                       >  1:1320558/1‑101 (MQ=255)
                                                atatCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATTAACTCCGGGAATGGCGCGGCGGGGCCGGTGGTGGACGCCATTGAAGCCCGCTTTAAAGcc                                                      >  1:1515549/1‑101 (MQ=255)
                                                 tatCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATCAACTCCGGGAACGGCGCAGCGGGTCCGGTGGTGGACGCTATCGAAGCCCGCTTTAACGccc                                                     >  2:1436977/1‑101 (MQ=255)
                                                   tCAGCGTCAAAGCCCTCCCGCCGCGCACGCGGGGGCGCAGCTCCAGGAACGGCGCAGCGGGTCCGGTGGTGGACGCTATCGGAGCCCGCTTTAACGCCCtt                                                   <  1:361440/101‑2 (MQ=255)
                                                   tCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATTAACTCCGGGAATGGCGCGGCGGGGCCGGTGGTGGACGCCATTGAAGCCCGCTTTAAAGCCCTc                                                   <  2:628943/101‑1 (MQ=255)
                                                   tCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATCAACTCCGGGAACGGCGCAGCGGGTCCGGTGGTGGACGCTATCGAAGCCCGCTTTAACGCCCTc                                                   >  2:1420911/1‑101 (MQ=255)
                                                     aaCGTCAAAAACCTCACGCCGCTCAAGCTGGCGATCAACTCCGGGAACAGCGCAGCGGGTCCGTTGGTGGGCGCTATCGAAGCCCGCTTTAACGCACTCga                                                 >  1:184854/1‑100 (MQ=255)
                                                      aCGTCAAAAACCTCACGCCGCTCAAGCTGGTGATCAACTCCGGGAACGGCGCAGCGGGTCCGGTGGTGGACGCTATCGAAGCCCGCTTTAACGCCCTCGgc                                                >  1:580170/1‑101 (MQ=255)
                                                       cGTCAAAAACCTCACGCCGCTCAAGCTGGTGATTAACTCCGGGAATGGCGCGGCGGGGCCGGTGGTGGACGCCATTGAAGCCCGCTTTAAAGCCCTCGgcg                                               >  1:337984/1‑101 (MQ=255)
                                                         tCAAAAACCTCACGCCGCTCAAGCTGGTGATCAACTCCGGGAACGGCGCAACGGGTCCGCTGGTGGACGCTATCGAAGCCAGCTTTAACGCCCTCGgcgcc                                             >  2:1498385/1‑100 (MQ=255)
                                                            aaaaCCTCACGCCGCTCAAGCTGGTGATTAACTCCGGGAATGGCGCGGCGGGGCCGGTGGTGGACGCCATTGAAGCCCGCTTTAAAGCCCTCGGCGCACCg                                          <  2:949179/101‑1 (MQ=255)
                                                            aaaaCCTCACGCCGCTCAAGCTGGTGATTAACTCCGGGAATGGCGCGGCGGGGCCGGTGGTGGACGCCATTGAAGCCCGCTTTAAAGCCCTCGGCGCACCg                                          >  2:1630367/1‑101 (MQ=255)
                                                             aaaCCTCACGCCGCTCAAGCTGGTGATTAACTCCGGGAATGGCGCGGCGGGGCCGGTGGTGGACGCCATTGAAGCCCGCTTTAAAGCCCTCGGCGCACCgg                                         >  1:550966/1‑101 (MQ=255)
                                                              aaCCTCACGCCGCTCAAGCTGGTGATCAACTCCGGGAACGGCGCAGCGGGTCCGGTGGTGGACGCTATCGAAGCCCGCTTTAACGCCCTCGGCGCTCCGGt                                        <  1:1589470/101‑1 (MQ=255)
                                                               aCCTCACGCCGCTCAAGCTGGTGATCAACTCCGGGAACGGCGCAGCGGGTCCGGTGGTGGACGCTATCGAAGCCCGCTTTAACGCCCTCGGCGCTCCGGTg                                       <  1:386621/101‑1 (MQ=255)
                                                                ccTCACGCCGCTCAAGCTGGTGATTAACTCCGGGAATGGCGCGGCGGGGCCGGTGGTGGACGCCATTGAAGCCCGCTTTAAAGCCCTCGGCGCACCGGTgg                                      <  2:1603354/101‑1 (MQ=255)
                                                                   cACGCCGCTCAAGCTGGTGATTAACTCCGGGAATGGCGCGGCGGGGCCGGTGGTGGACGCCATTGAAGCCCGCTTTAAAGCCCTCGGCGCACCGGTGGAAt                                   >  1:961511/1‑101 (MQ=255)
                                                                   cACGCCGCTCAAGCTGGTGATCAACTCCGGGAACGGCGCAGCGGGTCCGGTGGTGGACGCTATCGAAGCCCGCTTTAACGCCCTCGGCGCTCCGGTGGAAt                                   <  1:1089155/101‑1 (MQ=255)
                                                                   cACGCCGCTCAAGCTGGTGATCAACTCCGGGAACGGCGCAGCGGGTCCGGTGGTGGACGCTATCGAAGCCCGCTTTAACGCCCTCGGCGCTCCGGTGGAAt                                   <  2:1661207/101‑1 (MQ=255)
                                                                     cgccgcTCAAGCTGGTGATTAACTCCGGGAATGGCGCGGCGGGGCCGGTGGTGGACGCCATTGAAGCCCGCTTTAAAGCCCTCGGCGCACCGGTGGAATTa                                 <  1:1098958/101‑1 (MQ=255)
                                                                      gccgcTCAAGCTGGTGATCAACTCCGGGAACGGCGCAGCGGGTCCGGTGGTGGACGCTATCGAAGCCCGCTTTAACGCCCTCGGCGCTCCGGTGGAATTaa                                >  1:426943/1‑101 (MQ=255)
                                                                      gccgcTCAAGCTGGTGATCAACGCCGGGAACGGCGCAGCGGGTCCGGTGGTGGACGCTATCGAAGCCCGCTTTAACGCCCTCGGCGCTCCGGTGGAATTaa                                >  2:1120669/1‑101 (MQ=255)
                                                                        cgcTCAAGCTGGTGATTAACTCCGGGAATGGCGCGGCGGGGCCGGTGGTGGACGCCATTGAAGCCCGCTTTAAAGCCCTCGGCGCACCGGTGGAATTAATc                              >  1:503648/1‑101 (MQ=255)
                                                                        cgcTCAAGCTGGTGATCAACTCCGGGAACGGCGCAGCGGGGCCGGTGGTGGACGCTATCGAAGCCCGCTTTAACGCCCTCGGCGCTCCGGTGGAATTCATc                              >  2:1242465/1‑101 (MQ=255)
                                                                         gcTCAAGCTGGTGATTAACTCCGGGAATGGCGCGGCGGGGCCGGTGGTGGACGCCATTGAAGCCCGCTTTAAAGCCCTCGGCGCACCGGTGGAATTAATCa                             >  1:1716429/1‑101 (MQ=255)
                                                                          cTCAAGCTGGTGATCAACTCCGGGAACGGCGCAGCGGGTCCGGTGGTGGACGCTATCGAAGCCCGCTTTAACGCCCTCGGCGCTCCGGTGGAATTAATCaa                            >  2:290325/1‑101 (MQ=255)
                                                                          cTCAAGCTGGTGATCAACTCCGGGAACGGCGCAGCGGGTCCGGTGGTGGACGCTATCGAAGCCCGCTTTAACGCCCTCGGCGCTCCGGTGGAATTAATCaa                            >  1:242519/1‑101 (MQ=255)
                                                                            cAAGCTGGTGATTAACTCCGGGAATGGCGCGGCGGGGCCGGTGGTGGACGCCATTGAAGCCCGCTTTAAAGCCCTCGGCGCACCGGTGGAATTAATCAAAg                          <  2:1354222/101‑1 (MQ=255)
                                                                            cAAGCTGGTGATCAACTCCGGGAACGGCGCAGCGGGTCCGGTGGTGGACGCTATCGAAGCCCGCTTTAACGCCCTCGGCGCTCCGGTGGAATTAATCAAAg                          <  1:12859/101‑1 (MQ=255)
                                                                             aaGCTGGTGATTAACTCCGGGAATGGCGCGGCGGGGCCGGTGGTGGACGCCATTGAAGCCCGCTTTAAAGCCCTCGGCGCACCGGTGGAATTAATCAAAGt                         >  1:1673433/1‑101 (MQ=255)
                                                                                cTGGTGATTAACTCCGGGAATGGCGCGGCGGGGCCGGTGGTGGACGCCATTGAAGCCCGCTTTAAAGCCCTCGGCGCACCGGTGGAATTAATCAAAGTAca                      <  2:510209/101‑1 (MQ=255)
                                                                                cTGGTGATTAACTCCGGGAATGGCGCGGCGGGGCCGGTGGTGGACGCCATTGAAGCCCGCTTTAAAGCCCTCGGCGCACCGGTGGAATTAATCAAAGTAca                      <  1:1134491/101‑1 (MQ=255)
                                                                                  ggTGATCAACTCCGGGAACGGCGCAGCGGGTCCGGTGGTGGACGCTATCGAAGCCCGCTTTAACGCCCTCGGCGCTCCGGTGGAATTAATCAAAGTGcaca                    <  2:445956/101‑1 (MQ=255)
                                                                                   gTGATCAACTCCGGGAACGGCGCAGCGGGTCCGGTGGTGGACGCTATCGAAGCCCGCTTTAACGCCCTCGGCGCTCCGGTGGAATTAATCAAAGTGCacaa                   >  2:976019/1‑101 (MQ=255)
                                                                                    tGATTAACTCCGGGAATGGCGCGGCGGGGCCGGTGGTGGACGCCATTGAAGCCCGCTTTAAAGCCCTCGGCGCACCGGTGGAATTAATCAAAGTACacaac                  <  2:664072/101‑1 (MQ=255)
                                                                                    tGATCAACTCCGGGAACGGCGCAGCGGGTCCGGTGGTGGACGCTATCGAAGCCCGCTTTAACGCCCTCGGCGCTCCGGTGGAATTAATCAAAGTGCacaac                  >  2:1019985/1‑101 (MQ=255)
                                                                                     gATTAACTCCGGGAATGGCGCGGCGGGGCCGGTGGTGGGCGCCATTGAAGCCCGCTTTAAAGCCCTCGGCGCACCGGTGGAATTAATCAAAGTACacaaca                 >  1:1496220/1‑101 (MQ=255)
                                                                                     gATCAACTCCGGGAACGGCGCAGCGGGTCCGGTGGTGGACGCTATCGAAGCCCGCTTTAACGCCCTCGGCGCTCCGGTGGAATTAATCAAAGTGCacaaca                 <  1:1157624/101‑1 (MQ=255)
                                                                                     gATCAACTCCGGGAACGGCGCAGCGGGTCCGGTGGTGGACGCTATCGAAGCCCGCTTTAACGCCCTCGGCGCTCCGGTGGAATTAATCAAAGTGCacaaca                 >  2:249001/1‑101 (MQ=255)
                                                                                      attaaCTCCGGGAATGGCGCGGCGGGGCCGGTGGTGGACGCCATTGAAGCCCGCTTTAAAGCCCTCGGCGCACCGGTGGAATTAATCAAAGTACACAacac                >  2:756869/4‑101 (MQ=255)
                                                                                      attaaCTCCGGGAATGGCGCGGCGGGGCCGGTGGTGGACGCCATTGAAGCCCGCTTTAAAGCCCTCGGCGCACCGGTGGAATTAATCAAAGTACACAacac                >  2:383089/4‑101 (MQ=255)
                                                                                       tCAACTCCGGGAACGGCGCAGCGGGTCCGGTGGTGGACGCTATCGAAGCCCGCTTTAACGCCCTCGGCGCTCCGGTGGAATTAATCAAAGTGCACAACACg               <  2:146763/101‑1 (MQ=255)
                                                                                        taaCTCCGGGAATGGCGCGGCGGGGCCGGTGGTGGACGCCATTGAAGCCCGCTTTAAAGCCCTCGGCGCACCGGTGGAATTAATCAAAGTACACAACACGc              >  1:693354/2‑101 (MQ=255)
                                                                                         aaCTCCGGGAACGGCGCAGCGGGTCCGGTGGTGGACGCTATCGAAGCCCGCTTTAACGCCCTCGGCGCTCCGGTGGAATTAATCAAAGTGCACAACACGcc             <  2:935925/101‑1 (MQ=255)
                                                                                           cTCCGGGAATGGCGCGGCGGGGCCGGTGGTGGACGCCATTGAAGCCCGCTTTAAAGCCCTCGGCGCACCGGTGGAATTAATCAAAGTACACAACACGCCgg           >  2:126186/1‑101 (MQ=255)
                                                                                            tCCGGGAACGGCGCAGCGGGTCCGGTGGTGGACGCTATCGAAGCCCGCTTTAACGCCCTCGGCGCTCCGGTGGAATTAATCAAAGTGCACAACACGCCGGa          >  2:1500451/1‑101 (MQ=255)
                                                                                              cGGGAACGGCGCAGCGGGTCCGGTGGTGGACGCTATCGAAGCCCGCTTTAACGCCCTCGGCGCTCCGGTGGAATTAATCAAAGTGCACAACACGCCGGACg        >  1:181281/1‑101 (MQ=255)
                                                                                               gggAATGGCGCGGCGGGGCCGGTGGTGGACGCCATTGAAGCCCGCTTTAAAGCCCTCGGCGCACCGGTGGAATTAATCAAAGTACACAACACGCCGGACgg       <  2:746624/101‑1 (MQ=255)
                                                                                                  aaCGGCGCAGCGGGTCCGGTGGTGGACGCTATCGAAGCCCGCTTTAACGCCCTCGGCGCTCCGGTGGAATTAATCAAAGTGCACAACACGCCGGACGGCaa    >  2:234032/1‑101 (MQ=255)
                                                                                                    cGGCGCAGCGGGTCCGGTGGTGGACGCTATCGAAGCCCGCTTTAACGCCCTCGGCGCTCCGGTGGAATTAATCAAAGTGCACAACACGCCGGACGGCAAtt  <  1:1287467/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
ATCAGCAAATCAATCTGCGTGACGCTTACGTTGATCACCTGTTCGGTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATCAACTCCGGGAACGGCGCAGCGGGTCCGGTGGTGGACGCTATCGAAGCCCGCTTTAACGCCCTCGGCGCTCCGGTGGAATTAATCAAAGTGCACAACACGCCGGACGGCAATT  >  31/1973‑2173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑