Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*13312,7580TC78.9% 648.3 / 166.5 322intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (30/38);  new base C (125/129);  total (155/167)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.44e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 329 total aligned reads displayed.

AAAGCCCGTTCCCCGCAAGGACTGACGCCAGATAGTTTCTCTCCATGGCTGCTTTTTGCATCTTACGTCTTAACCCTGCCTTGAATACCTTATCATTCGTTAAAATATTAATAGCGATGTGCCGTATCCCTGAAAATAATTCTGCTGCATTTCCTCTTCTTATTTTGCAGTCGTCTTCATTCATTACCACGTCCAGACGCC  >  133/12658‑12858
                                                                                                    |                                                                                                    
 aaGCCCGCTCCCCGCAAGGACTGACGCGAGATAGTTTCTATCCATGGCTGCTTTTCGCATCTTACGTCTTAACCCTGCCTTGAATACCTTATCATTCGTTa                                                                                                     >  2:217799/1‑101 (MQ=17)
     ccGCTCCCCGCAAGGACTGACGCCAGGTAGTTTCTGTCCATGGCTGCTTTTCGCATCTTACGTCTTAACCCTGCCTTGAATACCTTCTCATTCGTCAAAat                                                                                                 >  2:486259/1‑101 (MQ=17)
      cgctCCCCGCAAGGACTGACGCCAGGTAGTTTCTGTCCATGGCTGCTTTTCGCATCTTACGTCTTAACCCTGCCTTGAATACCTTCTCATTCGTCAAAata                                                                                                >  2:1038549/4‑101 (MQ=17)
      cGTTCCCCGCAAGGACTGACGCCAGATAGTTTCTCTCCATGGCTGCTTTTTGCATCTTACGTCTTAACCCTGCCTTGAATACCTTATCATTAGTCAAAata                                                                                                >  2:165943/1‑101 (MQ=255)
        ctCCCCGCAAGGACTGACGCCAGGTAGTTTCTGTCCATGGCTGCTTTTCGCATCTTACGTCTTAACCCTGCCTTGAATACCTTCTCATTCGTCAAAATAtt                                                                                              <  2:954398/100‑1 (MQ=17)
        ctCCCCGCAAGGACTGACGCCAGATAGTTTCTGTCCATGGCTGCTTTTCGCATCTTACGTCTTAACCCTGCCTTGAATACCTTATCATTCGTCAAAATAtt                                                                                              >  2:617379/2‑101 (MQ=255)
         tCCCCGCAAGGACTGACGCGAGATAGTTTCTATCCATGGCTGCTTTTCGCATCTTACGTCTTAACCCTGCCTTGAATACCTTATCATTCGTTAAAATATTa                                                                                             <  2:43086/101‑1 (MQ=255)
         tCCCCGCAAGGACTGACGCCAGGTAGTTTCTGTCCATGGCTGCTTTTCGCATCTTACGTCTTAACCCTGCCTTGAATACCTTCTCATTCGTCAAAATATTa                                                                                             <  2:880844/101‑1 (MQ=17)
          ccccGCAAGGACTGACGCCAGGAAGTTTCTGTCCATGGCTGCTTTTCGCATCTTACGTCTTAACCCTGCCTTGAATACCTTCTCATTCGTCAAAATATTaa                                                                                            >  2:280702/1‑101 (MQ=18)
           cccGCAAGGACTGACGCCAGATAGTTTCTGTCCATGGCTGCTTTTCGCATCTTACGTCTTAACCCTGCCTTGAATACCTTATCATTCGTCAAAATATTAAt                                                                                           <  1:1214202/101‑1 (MQ=255)
           cccGCAAGGACTGACGCCAGATAGTTTCTGTCCATGGCTGCTTTTCGCATCTTACGTCTTAACCCTGCCTTGAATACCTTATCATTCGTCAAAATATTAAt                                                                                           <  1:505390/101‑1 (MQ=255)
           cccGCAAGGACTGACGCCAGATAGTTTCTCTCCATGGCTGCTTTTTGCATCTTACGTCTTAACCCTGCCTTGAATACCTTATCATTAGTCAAAATATTAAt                                                                                           <  2:145381/101‑1 (MQ=255)
            ccGCAAGGACTGACGCGAGATAGTTTCTATCCATGGCTGCTTTTCGCATCTTACGTCTTAACCCTGCCTTGAATACCTTATCATTCGTTAAAATATTAATa                                                                                          >  1:104351/1‑101 (MQ=255)
            ccGCAAGGACTGACGCGAGATAGTTTCTATCCATGGCTGCTTTTCGCATCTTACGTCTTAACCCTGCCTTGAATACCTTATCATTCGTTAAAATATTAATa                                                                                          <  2:1620640/101‑1 (MQ=255)
            ccGCAAGGACTGACGCCAGGTAGTTTCTGTCCATGGCTGCTTTTCGCATCTTACGTCTTAACCCTGCCTTGAATACCTTCTCATTCGTCAAAATATTAATa                                                                                          <  2:322346/101‑1 (MQ=18)
            ccGCAAGGACTGACGCCAGATAGTTTCTGTCCATGGCTGCTTTTCGCATCTTACGTCTTAACCCTGCCTTGAATACCTTATCATTCGTCAAAATATTAATa                                                                                          <  2:81138/101‑1 (MQ=255)
            ccGCAAGGACTGACGCCAGATAGTTTCTGTCCATGGCTGCTTTTCGCATCTTACGTCTTAACCCTGCCTTGAATACCTTATCATTCGTCAAAATATTAATa                                                                                          <  1:1258986/101‑1 (MQ=255)
            ccGCAAGGACTGACGCCAGATAGTTTCTCTCCATGGCTGCTTTTTGCATCTTACGTCTTATCCCTGCCTTGAATACCTTATCATTAGTCAAAATATTAATa                                                                                          <  1:1661513/101‑1 (MQ=255)
             cGCAAGGACTGACGCGAGATAGTTTCTATCCATGGCTGCTTTTCGCATCTTACGTCTTAACCCTGCCTTGAATACCTTATCATTCGTTAAAATATTAATAg                                                                                         >  1:409585/1‑101 (MQ=255)
             cGCAAGGACTGACGCGAGATAGTTTCTATCCATGGCTGCTTTTCGCATCTTACGTCTTAACCCTGCCTTGAATACCTTATCATTCGTTAAAATATTAATAg                                                                                         <  1:1462481/101‑1 (MQ=255)
                 aGGACTGACGCCAGATAGTTTCTCTCCATGGCTGCTTTTTGCATCTTACGTCTTAACCCTGCCTTGAATACCTTATCATTAGTCAAAATATTAATAGCGAt                                                                                     <  1:780320/101‑1 (MQ=255)
                  ggACTGACGCCAGGTAGTTTCTGTCCATGGCTGCTTTTCGCATCTTACGTCTTAACCCTGCCTTGAATACCTTCTCATTCGTCAAAATATTAATAGCAAtg                                                                                    >  1:260169/1‑101 (MQ=18)
                  ggACTGACGCCAGGTAGTTTCTGTCCATGGCTGCTTTTCGCATCTTACGTCTTAACCCTGCCTTGAATACCTTCTCATTCGTCAAAATATTAATAGCAAtg                                                                                    <  1:801726/101‑1 (MQ=18)
                  ggACTGACGCCAGATAGTTTCTGTCCATGGCTGCTTTTCGCATCTTACGTCTTAACCCTGCCTTGAATACCTTATCATTCGTCAAAATATTAATAGCAAtg                                                                                    <  2:1266727/101‑1 (MQ=21)
                   gACTGACGCCAGATAGTTTCTGTCCATGGCTGCTTTTCGCATCTTACGTCTTAACCCTGCCTTGAATACCTTATCATTCGTCAAAATATTAATAGCAAtgt                                                                                   <  1:1063781/101‑1 (MQ=37)
                     cTGACGCCAGGTAGTTTCTGTCCATGGCTGCTTTTCGCATCTTACGTCTTAACCCTGCCTTGAATACCTTCTCATTCGTCAAAATATTAATAGCAATGTGc                                                                                 >  1:1032862/1‑101 (MQ=21)
                      tGACGCCAGATAGTTTCTGTCCATGGCTGCTTTTCGCATCTTACGTCTTAACCCTGCCTTGAATACCTTATCATTCGTCAAAATATTAATAGCAATGTGcc                                                                                >  1:1121841/1‑101 (MQ=21)
                      tGACGCCAGATAGTTTCTCTCCATGGCTGCTTTTTGCATCTTACGTCTTAACCCTGCCTTGAATACCTTATCATTAGTCAAAATATTAATAGCGATGTGcc                                                                                <  1:202925/101‑1 (MQ=255)
                       gACGCCAGATAGTTTCTCTCCATGGCTGCTTTTTGCATCTTACGTCTTAACCCTGCCTTGAATACCTTATCATTAGTCAAAATATTAATAGCGATGTGCCg                                                                               >  2:1577873/1‑101 (MQ=255)
                       gACGCCAGATAGTTTCTCTCCATGGCTGCTTTTTGCATCTTACGTCTTAACCCTGCCTTGAATACCTTATCATTAGTCAAAATATTAATAGCGATGTGCCg                                                                               <  1:644959/101‑1 (MQ=255)
                         cGCCAGGTAGTTTCTGTCCATGGCTGCTTTTCGCATCTTACGTCTTAACCCTGCCTTGAATACCTTCTCATTCGTCAAAATATTAATAGCAATGTGCCGTa                                                                             >  1:1486435/1‑101 (MQ=21)
                           ccAGATAGTTTCTGTCCATGGCTGCTTTTCGCATCTTACGTCTTAACCCTGCCTTGAATACCTTATCATTCGTCAAAATATTAATAGCAATGTGCCGTATc                                                                           <  2:288691/101‑1 (MQ=25)
                             agttAGGTTCTGTCAATGGCTGCTTTTCGCATGTTACGTCTTCACCCTGCCTTGAATACTTGCTCTTTCGTCAAAATATTAGTAGCAAGGTGCCGTATccc                                                                         <  2:860938/98‑1 (MQ=16)
                              ggtAGTTTCTGTCCATGGCTGCTTTTCGCATCTTACGTCTTAACCCTGCCTTGAATACCTTCTCATTCGTCAAAATATTAATAGCAATGTGCCGTATCCCt                                                                        <  2:1004923/99‑1 (MQ=25)
                              ggtAGTTTCTGTCCATGGCTGCTTTTCGCATCTTACGTCTTAACCCTGCCTTGAATACCTTCTCATTCGTCAAAATATTAATAGCAATGTGCCGTATCCCt                                                                        >  1:168364/3‑101 (MQ=25)
                               gtAGTTTCTGTCCATGGCTGCTTTTCGCATCTTACGTCTTAACCCTGCCTTGAATACCTTCTCATTCGTCAAAATATTAATAGCAATGTGCCGTATCCCTg                                                                       >  2:392862/2‑101 (MQ=25)
                               aTAGTTTCTGTCCATGGCTGCTTTTCGCATCTTACGTCTTAACCCTGCCTTGAATACCTTATCATTCGTCAAAATATTAATAGCAATGTGCCGTATCCCTg                                                                       >  2:1155155/1‑101 (MQ=37)
                               aTAGTTTCTATCCATGGCTGCTTTTCGCATCTTACGTCTTAACCCTGCCTTGAATACCTTATCATTCGTTAAAATATTAATAGCGATGTGCCGTATCCCTg                                                                       <  1:1388264/101‑1 (MQ=255)
                                  gTTTCTGTCCATGGCTGCTTTTCGCATCTTACGTCTTAACCCTGCCTTGAATACCTTCTCATTCGTCAAAATATTAATAGCAATGTGCCGTATCCCTGaaa                                                                    >  1:530526/1‑101 (MQ=25)
                                   tttCTATCCATGGCTGCTTTTCGCATCTTACGTCTTAACCCTGCCTTGAATACCTTATCATTCGTTAAAATATTAATAGCGATGTGCCGTATCCCTGaaaa                                                                   <  1:703232/101‑1 (MQ=255)
                                    ttCTGTCCATGGCTGCTTTTCGCATCTTACGTCTTAACCCTGCCTTGAATACCTTATCATTCGTCAAAATATTAATAGCAATGTGCCGTATCCCTGAAaat                                                                  >  1:1245672/1‑101 (MQ=37)
                                      ctatcCATGGCTGCTTTTCGCATCTTACGTCTTAACCCTGCCTTGAATACCTTATCATTCGTTAAAATATTAATAGCGATGTGCCGTATCCCTGAAaataa                                                                >  1:209049/4‑101 (MQ=255)
                                       tgtcCATGGCTGCTTTTCGCATCTTACGTCTTAACCCTGCCTTGAATACCTTCTCATTCGTCAAAATATTAATAGCAATGTGCCGTATCCCTGAAaataat                                                               >  1:329628/3‑101 (MQ=37)
                                       tatcCATGGCTGCTTTTCGCATCTTACGTCTTAACCCTGCCTTGAATACCTTATCATTCGTTAAAATATTAATAGCGATGTGCCGTATCCCTGAAaataat                                                               <  2:1355744/99‑1 (MQ=255)
                                         tcCATGGCTGCTTTTCGCATCTTACGTCTTAACCCTGCCTTGAATACCTTATCATTCGTCAAAATATTAATAGCAATGTGCCGTATCCCTGAAAATAATTc                                                             <  2:728347/101‑1 (MQ=255)
                                          ccATGGCTGCTTTTTGCATCTTACGTCTTAACCCTGCCTTGAATACCTTATCATTAGTCAAAATATTAATAGCGATGTGCCGTATCCCTGAAAATAATTct                                                            <  1:1586442/101‑1 (MQ=255)
                                          ccATGGCTGCTTTTCGCATCTTACGTCTTAACCCTGCCTTGAATACCTTATCATTCGTCAAAATATTAATAGCAATGTGCCGTATCCCTGAAAATAATTct                                                            <  2:1471146/101‑1 (MQ=255)
                                            aTGGCTGCTTTTTGCATCTTACGTCTTAACCCTGCCTTGAATACCTTATCATTAGTCAAAATATTAATAGCGATGTGCCGTATCCCTGAAAATAATTctgc                                                          <  2:533564/101‑1 (MQ=255)
                                             tGGCTGCTTTTTGCATCTTACGTCTTAACCCTGCCTTGAATACCTTATCATTAGTCAAAATATTAATAGCGATGTGCCGTATCCCTGAAAATAATTctgct                                                         <  2:636296/101‑1 (MQ=255)
                                             tGGCTGCTTTTCGCATCTTACGTCTTAACCCTGCCTTGAATACCTTATCATTCGTCAAAATATTAATAGCAATGTGCCGTATCCCTGAAAATAATTctgct                                                         >  1:910938/1‑101 (MQ=255)
                                              ggCTGCTTTTCGCATCTTACGTCTTAACCCTGCCTTGAATACCTTCTCATTCGTCAAAATATTAATAGCAATGTGCCGTATCCCTGAAAATAATTctgctg                                                        >  2:558307/1‑101 (MQ=38)
                                               gctgctTTTCGCATCTTACGTCTTAACCCTGCCTTGAATACCTTCTCATTCGTCAAAATATTAATAGCAATGTGCCGTATCCCTGAAAATAATTCtgctgc                                                       <  2:260169/101‑1 (MQ=39)
                                               gctgctTTTCGCATCTTACGTCTTAACCCTGCCTTGAATACCTTCTCATTCGTCAAAATATTAATAGCAATGTGCCGTATCCCTGAAAATAATTCtgctgc                                                       >  1:854572/1‑101 (MQ=38)
                                                ctgctTTTTGCATCTTACGTCTTAACCCTGCCTTGAATACCTTATCATTAGTCAAAATATTAATAGCGATGTGCCGTATCCCTGAAAATAATTCTGCTGCa                                                      <  1:361918/101‑1 (MQ=255)
                                                ctgctTTTCGCATCTTACGTCTTAACCCTGCCTTGAATACCTTATCATTCGTCAAAATATTAATAGCAATGTGCCGTATCCCTGAAAATAATTCTGCTGCa                                                      >  1:1009896/1‑101 (MQ=255)
                                                 tgctTTTCGCATCTTACGTCTTAACCCTGCCTTGAATACCTTATCATTCGTTAAAATATTAATAGCGATGTGCCGTATCCCTGAAAATAATTCtgcggcgt                                                     >  1:958892/1‑99 (MQ=255)
                                                  gctTTTTGCATCTTACGTCTTAACCCTGCCTTGAATACCTTATCATTAGTCAAAATATTAATAGCGATGTGCCGTATCCCTGAAAATAATTCTGCTGCAtt                                                    <  2:140575/101‑1 (MQ=255)
                                                    ttttcGCATCTTACGTCTTAACCCTGCCTTGAATACCTTCTCATTCGTCAAAATATTAATAGCAATGTGCCGTATCCCTGAAAATAATTCTGCTGCGTTTc                                                  >  2:582295/1‑101 (MQ=255)
                                                     tttcGCATCTTACGTCTTAACCCTGCCTTGAATACCTTCTCATTCGTCAAAATATTAATAGCAATGTGCCGTATCCCTGAAAATAATTCTGCTGCGTTTcc                                                 <  1:1153602/101‑1 (MQ=255)
                                                       ttGCATCTTACGTCTTAACCCTGCCTTGAATACCTTATCATTAGTCAAAATATTAATAGCGATGTGCCGTATCCCTGAAAATAATTCTGCTGCATTTCctc                                               <  2:1162799/101‑1 (MQ=255)
                                                       ttGCATCTTACGTCTTAACCCTGCCTTGAATACCTTATCATTAGTCAAAATATTAATAGCGATGTGCCGTATCCCTGAAAATAATTCTGCTGCATTTCctc                                               <  2:45408/101‑1 (MQ=255)
                                                            tCTTACGTCTTAACCCTGCCTTGAATACCTTATCATTCGTCAAAATATTAATAGCAATGTGCCGTATCCCTGAAAATAATTCTGCTGCATTTCCTcttctt                                          >  2:315373/1‑101 (MQ=255)
                                                             cTTACGTCTTAACCCTGCCTTGAATACCTTATCATTCGTTAAAATATTAATAGCGATGTGCCGTATCCCTGAAAATAATTCTGCGGCGTTTCCTCTTCTTa                                         <  2:456475/101‑1 (MQ=255)
                                                             cTTACGTCTTAACCCTGCCTTGAATACCTTATCATTAGTCAAAATATTAATAGCGATGTGCCGTATCCCTGAAAATAATTCTGCTGCATTTCCTCTTCTTa                                         >  1:249003/1‑101 (MQ=255)
                                                              ttACGTCTTAACCCTGCCTTGAATACCTTATCATTCGTCAAAATATTAATAGCAATGTGACGTATCCCTGAAAATAATTCTGCTGCATTTCCTCTTCTTAt                                        <  1:1568487/101‑1 (MQ=255)
                                                              ttACGTCTTAACCCTGCCTTGAATACCTTATCATTAGTCAAAATATTAATAGCGATGTGCCGTATCCCTGAAAATAATTCTGCTGCATTTCCTCTTCTTAt                                        <  1:296910/101‑1 (MQ=255)
                                                                  gTCTTAACCCTGCCTTGAATACCTTATCATTCGTCAAAATATTAATAGCAATGTGCCGTATCCCTGAAAATAATTCTGCTGCATTTCCTCTTCTTATTTTg                                    >  1:898159/1‑101 (MQ=255)
                                                                    cTTAACCCTGCCTTGAATACCTTATCATTCGTTAAAATATTAATAGCGATGTGCCGTATCCCTGAAAATAATTCTGCGGCGTTTCCTCTTCTTATTTTGCa                                  <  1:916822/101‑1 (MQ=255)
                                                                    cTTAACCCTGCCTTGAATACCTTATCATTCGTTAAAATATTAATAGCGATGTGCCGTATCCCTGAAAATAATTCTGCGGCGTTTCCTCTTCTTATTTTGCa                                  >  2:245323/1‑101 (MQ=255)
                                                                    cTTAACCCTGCCTTGAATACCTTATCATTCGTCAAAATATTAATAGCAATGTGCCGTATCCCTAAAAATAATTCTGCTGCATTTCCTCTTCTTATTTTGCa                                  <  2:1121836/101‑1 (MQ=255)
                                                                      tAACCCTGCCTTGAATACCTTATCATTCGTCAAAATATTAATAGCAATGTGCCGTATCCCTGAAAATAATTCTGCTGCATTTCCTCTTCTTATTTTGCAgt                                <  2:539908/101‑1 (MQ=255)
                                                                      tAACCCTGCCTTGAATACCTTATCATTAGTCAAAATATTAATAGCGATGTGCCGTATCCCTGAAAATAATTCTGCTGCATTTCCTCTTCTTATTTTGCAgt                                <  1:1290765/101‑1 (MQ=255)
                                                                      tAACCCTGCCTTGAATACCTTATCATTAGTCAAAATATTAATAGCGATGTGCCGTATCCCTGAAAATAATTCTGCTGCATTTCCTCTTCTTATTTTGCAgt                                <  2:1201752/101‑1 (MQ=255)
                                                                       aaCCCTGCCTTGAATACCTTATCATTAGTCAAAATATTAATAGCGATGTGCCGTATCCCTGAAAATAATTCTGCTGCATTTCCTCTTCTTATTTTGCAgtc                               >  2:1249814/1‑101 (MQ=255)
                                                                          ccTGCCTTGAATACCTTATCATTAGTCAAAATATTAATAGCGATGTGCCGTATCCCTGAAAATAATTCTGCTGCATTTCCTCTTCTTATTTTGCAgtcgtc                            >  1:1351539/1‑101 (MQ=255)
                                                                            tGCCTTGAATACCTTATCATTCGTCAAAATATTAATAGCAATGTGCCGTATCCCTGAAAATAATTCTGCTGCATTTCCTCTTCTTATTTTGCAGTCGTCtt                          >  2:408901/1‑101 (MQ=255)
                                                                              ccTTGAATACCTTCTCATTCGTCAAAATATTAATAGCAATGTGCCGTATCCCTGAAAATAATTCTGCTGCGTTTCCTCTTCTTATTTTGTAGTCGTCttca                        <  1:938515/101‑1 (MQ=255)
                                                                                ttGAATACCTTATCATTAGTCAAAATATTAATAGCGATGTGCCGTATCCCTGAAAATAATTCTGCTGCATTTCCTCTTCTTATTTTGCAGTCGTCttcatt                      <  2:1383856/101‑1 (MQ=255)
                                                                                ttGAATACCTTATCATTAGTCAAAATATTAATAGCGATGTGCCGGATCCCTGAAAATAATTCTGATGCATTTCCTCTACTATTACCGCAGCCGTCttcatt                      >  2:1310972/1‑101 (MQ=255)
                                                                                  gAATACCTTATCATTCGTCAAAATATTAATAGCAATGTGCCGTATCCCTGAAAATAATTCTGCTGCATTTCCTCTTCTTATTTTGCAGTCGTCttcattca                    >  1:15056/1‑101 (MQ=255)
                                                                                  gAATACCTTATCATTCGTCAAAATATTAATAGCAATGTGCCGTATCCCTGAAAATAATTCTGCTGCATTTCCTCTTCTTATTTTGCAGTCGTCttcattca                    <  1:1179109/101‑1 (MQ=255)
                                                                                   aaTACCTTATCATTAGTCAAAATATTAATAGCGATGTGCCGTATCCCTGAAAATAATTCTGCTGCATTTCCTCTTCTTATTTTGCAGTCGTCTtcattcat                   <  2:209574/101‑1 (MQ=255)
                                                                                    aTACCTTATCATTCGTCAAAATATTAATAGCAATGTGCCGTATCCCTGAAAATAATTCTGCTGCATTTCCTCTTCTTATTTTGCAGTCGTCTTcattcatt                  <  2:1285707/101‑1 (MQ=255)
                                                                                    aTACCTTATCATTCGTCAAAATATTAATAGCAATGTGCCGTATCCCTGAAAATAATTCTGCTGCATTTCCTCTTCTTATTTTGCAGTCGTCTTcattcatt                  <  2:1718634/101‑1 (MQ=255)
                                                                                    aTACCTTATCATTAGTCAAAATATTAATAGCGATGTGCCGTATCCCTGAAAATAATTCTGCTGCATTTCCTCTTCTTATTTTGCAGTCGTCTTcattcatt                  >  2:690481/1‑101 (MQ=255)
                                                                                     tACCTTATCATTCGTCAAAATATTAATAGCAATGTGCCGTATCCCTGAAAATAATTCTGCTGCATTTCCTCTTCTTATTTTGCAGTCGTCTTCATTCATTa                 <  1:1336303/101‑1 (MQ=255)
                                                                                      aCCTTCTCATTCGTCAAAATATTAATAGCAATGTGCCGTATCCCTGAAAATAATTCTGCTGCGTTTCCTCTTCTTATTTTGTAGTCGTCTTCATTCATTAc                <  1:558312/101‑1 (MQ=255)
                                                                                      aCCTTATCATTCGTTAAAATATTAATAGCGATGTGCCGTATCCCTGAAAATAATTCTGCGGCGTTTCCTCTTCTTATTTTGCAGTCGTCTTCATTCATTAc                <  1:217799/101‑1 (MQ=255)
                                                                                       ccTTATCATTCGTCAAAATATTAATAGCAATGTGCCGTATCCCTGAAAATAATTCTGCTGCATTTCCTCTTCTTATTTTGCAGTCGTCTTCATTCATTAcc               >  1:440533/1‑101 (MQ=255)
                                                                                        cTTCTCATTCGTCAAAATATTAATAGCAATGTGCCGTATCCCTGAAAATAATTCTGCTGCGTTTCCTCTTCTTATTTTGTAGTCGTCTTCATTCATTACCa              >  2:3518/1‑101 (MQ=255)
                                                                                         ttATCATTCGTCAAAATATTAATAGCAATGTGCCGTATCCCTGAAAATAATTCTGCTGCATTTCCTCTTCTTATTTTGCAGTCGTCTTCATTCATTACCAc             >  2:1511802/1‑101 (MQ=255)
                                                                                         ttATCATTAGTCAAAATATTAATAGCGATGTGCCGTATCCCTGAAAATAATTCTGCTGCATTTCCTCTTCTTATTTTGCAGTCGTCTTCATTCATTACCAc             <  1:162859/101‑1 (MQ=255)
                                                                                          tctCATTCGTCAAAATATTAATAGCAATGTGCCGTATCCCTGAAAATAATTCTGCTGCGTTTCCTCTTCTTATTTTGTAGTCGTCTTCATTCATTACCACg            >  1:588445/3‑101 (MQ=255)
                                                                                          tATCATTCGTCAAAATATTAATAGCAATGTGCCGTATCCCTGAAAATAATTCTGCTGCATTTCCTCTTCTTATTTTGCAGTCGTCTTCATTCATTACCACg            >  2:761118/1‑101 (MQ=255)
                                                                                            tCATTCGTTAAAATATTAATAGCGATGTGCCGTATCCCTGAAAATAATTCTGCGGCGTTTCCTCTTCTTATTTTGCAGTCGTCTTCATTCATTACCACGTc          >  2:194127/1‑101 (MQ=255)
                                                                                            tCATTAGTCAAAATATTAATAGCGATGTGCCGTATCCCTGAAAATAATTCTGCTGCATTTCCTCTTCTTATTTTGCAGTCGTCTTCATTCATTACCACGTc          >  2:923775/1‑101 (MQ=255)
                                                                                              aTTCGTCAAAATATTAATAGCAATGTGCCGTATCCCTGAAAATAATTCTGCTGCGTTTCCTCTTCTTATTTTGTAGTCGTCTTCATTCATTACCACGTCCa        >  1:758554/1‑101 (MQ=255)
                                                                                               ttCGTTAAAATATTAATAGCGATGTGCCGTATCCCTGAAAATAATTCTGCGGCGTTTCCTCTTCTTATTTTGCAGTCGTCTTCATTCATTACCACGTCCat       <  1:1282715/101‑2 (MQ=255)
                                                                                                 cGTCAAAATATTAATAGCAATGTGCCGTATCCCTGAAAATAATTCTGCTGCGTTTCCTCTTCTTATTTTGTAGTCGTCTTCATTCATTACCACGTCCAGAc     >  1:450523/1‑101 (MQ=255)
                                                                                                 cGTCAAAATATTAATAGCAATGTGCCGTATCCCTGAAAATAATTCTGCTGCATTTCCTCTTCTTATTTTGCAGTCGTCTTCATTCATTACCACGTCCAGAc     <  1:183310/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
AAAGCCCGTTCCCCGCAAGGACTGACGCCAGATAGTTTCTCTCCATGGCTGCTTTTTGCATCTTACGTCTTAACCCTGCCTTGAATACCTTATCATTCGTTAAAATATTAATAGCGATGTGCCGTATCCCTGAAAATAATTCTGCTGCATTTCCTCTTCTTATTTTGCAGTCGTCTTCATTCATTACCACGTCCAGACGCC  >  133/12658‑12858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑