Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	4,384,430	C→A	S29Y (TCT→TAT)	yjeB →	predicted DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	4,384,430	0	C	A	100.0%	268.9 / NA	72	S29Y (TCT→TAT)	yjeB	predicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (41/31);  total (41/31)

TTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTCTGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCTACGTGACTGCTGTTCGTGGAAA  >  REL606/4384332‑4384526                                                                                                   |                                                                                                 ttttGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATg                                                                                                >  2:369376/1‑101 (MQ=255)  tttGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGa                                                                                               >  1:940444/1‑101 (MQ=255)  tttGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGa                                                                                               >  2:861172/1‑101 (MQ=255)   ttGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGaa                                                                                              <  2:1441804/101‑1 (MQ=255)    tGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAg                                                                                             <  1:832600/101‑1 (MQ=255)      aGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGtg                                                                                           <  2:910520/101‑1 (MQ=255)      aGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGtg                                                                                           >  2:1567738/1‑101 (MQ=255)        gTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTCCATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGtgac                                                                                         <  2:558251/101‑1 (MQ=255)         ttGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGtgact                                                                                        <  2:1111810/101‑1 (MQ=255)            aTGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGtgactgac                                                                                     <  1:863424/101‑1 (MQ=255)              gtgCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGt                                                                                   >  2:1549173/1‑101 (MQ=255)               tgCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTc                                                                                  >  2:584976/1‑101 (MQ=255)                gCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCt                                                                                 <  1:1007652/101‑1 (MQ=255)                gCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCt                                                                                 <  2:1241190/101‑1 (MQ=255)                gCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCt                                                                                 <  2:1210808/101‑1 (MQ=255)                 cAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTa                                                                                <  2:36663/101‑1 (MQ=255)                 cAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTa                                                                                <  2:1223602/101‑1 (MQ=255)                  aGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTAc                                                                               >  1:1701909/1‑101 (MQ=255)                   gTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTAca                                                                              <  2:98256/101‑2 (MQ=255)                   gTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTAca                                                                              <  2:1069226/101‑2 (MQ=255)                      aaCGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCg                                                                           >  1:1291372/1‑101 (MQ=255)                      aaCGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCg                                                                           >  1:1517852/1‑101 (MQ=255)                       aCGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGt                                                                          >  2:1692525/1‑101 (MQ=255)                          aGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGtctc                                                                       <  2:1396320/101‑1 (MQ=255)                          aGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGtctc                                                                       >  1:1387696/1‑101 (MQ=255)                           gTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTcc                                                                      <  2:370511/101‑1 (MQ=255)                              tCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGt                                                                   >  1:785688/1‑101 (MQ=255)                                aCTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTaa                                                                 >  1:1392516/1‑101 (MQ=255)                                aCTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTaa                                                                 >  1:1413690/1‑101 (MQ=255)                                aCTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTaa                                                                 >  1:350718/1‑101 (MQ=255)                                    attacggattacgTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCat                                                             >  2:195087/1‑101 (MQ=255)                                    attacggattacgTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCat                                                             <  1:438265/101‑1 (MQ=255)                                    attacggattacgTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCat                                                             >  1:4078/1‑101 (MQ=255)                                     ttacggattacgTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCata                                                            <  1:889158/101‑1 (MQ=255)                                               cgTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAaat                                                  >  2:1287956/1‑101 (MQ=255)                                               cgTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAaat                                                  >  1:1289753/1‑101 (MQ=255)                                                gTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAaata                                                 >  1:348409/1‑101 (MQ=255)                                                      tGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATaatcaat                                           >  2:68256/1‑101 (MQ=255)                                                      tGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATaatcaat                                           >  1:373346/1‑101 (MQ=255)                                                      tGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATaatcaat                                           <  1:1164039/101‑1 (MQ=255)                                                       gATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATaatcaatc                                          >  1:288843/1‑101 (MQ=255)                                                       gATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATaatcaatc                                          <  2:89038/101‑1 (MQ=255)                                                         tCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAAtcaatcaa                                        <  1:764317/101‑1 (MQ=255)                                                         tCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAAtcaatcaa                                        <  1:1549179/101‑1 (MQ=255)                                                          cTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAAc                                       <  1:380653/101‑1 (MQ=255)                                                           tACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACt                                      <  2:112389/101‑1 (MQ=255)                                                                   gTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTg                              >  1:651494/1‑101 (MQ=255)                                                                   gTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTg                              >  2:579096/1‑101 (MQ=255)                                                                   gTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTg                              >  2:975337/1‑101 (MQ=255)                                                                   gTCATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTg                              >  1:1109585/1‑101 (MQ=255)                                                                     cATTGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGcc                            >  1:95087/1‑101 (MQ=255)                                                                       ttGCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCgg                          <  2:173610/101‑1 (MQ=255)                                                                         gCCAGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCt                        <  1:1224611/101‑1 (MQ=255)                                                                            aGAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCTACg                     <  2:968146/101‑1 (MQ=255)                                                                             gAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCTACGt                    <  1:1335344/101‑1 (MQ=255)                                                                             gAAGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCTACGt                    >  1:717891/1‑101 (MQ=255)                                                                              aaGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCTACGTg                   >  2:1119052/1‑101 (MQ=255)                                                                              aaGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCTACGTg                   <  1:326184/101‑1 (MQ=255)                                                                               aGGGCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCTACGTGa                  <  1:1337618/101‑1 (MQ=255)                                                                                gggCGGATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCTACGTGAc                 <  2:1109698/101‑1 (MQ=255)                                                                                    ggATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCTACGTGActgct             <  2:1701903/101‑1 (MQ=255)                                                                                    ggATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCTACGTGActgct             >  1:564948/1‑101 (MQ=255)                                                                                     gATGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCTACGTGActgctg            >  2:1077135/1‑101 (MQ=255)                                                                                       tGACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCTACGTGACTGCTGtt          >  2:285858/1‑101 (MQ=255)                                                                                        gACCAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCTACGTGACTGCTGTTc         >  2:1515234/1‑101 (MQ=255)                                                                                          ccAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCTACGTGACTGCTGTTCGt       >  2:1252793/1‑101 (MQ=255)                                                                                           cAGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCTACGTGACTGCTGTTCGTg      >  1:1336761/1‑101 (MQ=255)                                                                                            aGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCTACGTGACTGCTGTTCGTgg     >  1:1198708/1‑101 (MQ=255)                                                                                            aGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCTACGTGACTGCTGTTCGTgg     >  1:1136949/1‑101 (MQ=255)                                                                                            aGTATTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCTACGTGACTGCTGTTCGTgg     >  1:1542054/1‑101 (MQ=255)                                                                                               aTTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCTACGTGACTGCTGTTCGTGGaaa  >  1:779528/1‑101 (MQ=255)                                                                                               aTTTATGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCTACGTGACTGCTGTTCGTGGaaa  >  1:1395965/1‑101 (MQ=255)                                                                                                   |                                                                                                 TTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATTACGTGCGCTGATCTACATGGCGTCATTGCCAGAAGGGCGGATGACCAGTATTTCTGAAGTGACTGACGTCTACGGCGTCTCCCGTAATCATATGGTCAAAATAATCAATCAACTTAGTCGTGCCGGCTACGTGACTGCTGTTCGTGGAAA  >  REL606/4384332‑4384526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑