Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	430,835	C→T	intergenic (‑48/‑108)	insL‑2 ← / → lon	putative transposase insL for insertion sequence IS186/DNA‑binding ATP‑dependent protease La

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	430,835	0	C	T	100.0%	188.9 / NA	52	intergenic (‑48/‑108)	insL‑2/lon	putative transposase insL for insertion sequence IS186/DNA‑binding ATP‑dependent protease La
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/19);  total (33/19)

GCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCATCTGATTACCTGGCGGAAATTAAACTAA  >  REL606/430750‑430931                                                                                      |                                                                                                 gCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACa                                                                                   <  1:1180163/101‑1 (MQ=25) gCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACa                                                                                   <  1:1321690/101‑1 (MQ=25)  ccAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACAt                                                                                  <  2:1657960/101‑1 (MQ=25)  ccAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACAt                                                                                  >  1:1580854/1‑101 (MQ=25)    aGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATcc                                                                                >  2:44487/1‑101 (MQ=255)    aGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATcc                                                                                <  1:302359/101‑1 (MQ=255)    aGAATTGCTGACCAGNTATCGNGANAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATcc                                                                                <  2:855884/101‑1 (MQ=255)      aaTTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATcccc                                                                              <  1:358209/101‑1 (MQ=255)         tGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTGATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCata                                                                           >  2:113711/1‑101 (MQ=255)         tGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCata                                                                           >  2:979346/1‑101 (MQ=255)           cTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCAtata                                                                         >  2:813647/1‑101 (MQ=255)            tGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATAc                                                                        >  1:855839/1‑101 (MQ=255)            tGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATAc                                                                        >  2:551960/1‑101 (MQ=255)             gACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACt                                                                       >  2:1598289/1‑101 (MQ=255)              aCCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTg                                                                      >  1:860304/1‑101 (MQ=255)                cAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGAc                                                                    >  2:1681825/1‑101 (MQ=255)                cAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGAc                                                                    >  1:607586/1‑101 (MQ=255)                   ttATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTa                                                                 >  1:1175865/1‑101 (MQ=255)                    tATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTAc                                                                >  1:807757/1‑101 (MQ=255)                            gagTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAAt                                                        >  1:1312207/1‑101 (MQ=255)                             agTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATa                                                       <  1:913939/101‑1 (MQ=255)                                aaTTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGAt                                                    >  2:88345/1‑101 (MQ=255)                                  ttCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATgg                                                  <  2:1038352/101‑1 (MQ=255)                                  ttCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATgg                                                  <  1:490450/101‑1 (MQ=255)                                   tCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGc                                                 <  2:1312201/101‑1 (MQ=255)                                          cACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGc                                          >  1:471006/1‑101 (MQ=255)                                           aCGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGca                                         <  2:1602978/101‑1 (MQ=255)                                            cGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGcac                                        <  1:986759/101‑1 (MQ=255)                                            cGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGcac                                        >  1:257282/1‑101 (MQ=255)                                              ttAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAg                                      >  2:1389500/1‑101 (MQ=255)                                               tAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGAACATGTTAATAGATGGCGTGAAGAACAGt                                     >  2:919793/1‑101 (MQ=255)                                                aaaTCATATCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTc                                    >  2:224721/1‑101 (MQ=255)                                                     atatCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTc                               >  1:497896/1‑101 (MQ=255)                                                       atCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCAt                             >  2:368450/1‑101 (MQ=255)                                                       atCAGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCAt                             >  1:608722/1‑101 (MQ=255)                                                          aGGCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCATCTg                          >  1:775427/1‑101 (MQ=255)                                                            gCGTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCATCTGAt                        <  1:703336/101‑1 (MQ=255)                                                              gTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCATCTGATTa                      <  2:690297/101‑1 (MQ=255)                                                              gTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCATCTGATTa                      >  1:1534066/1‑101 (MQ=255)                                                              gTAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCATCTGATTa                      >  1:1691236/1‑101 (MQ=255)                                                               tAATACCACAGCCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCATCTGATTAc                     >  2:1145806/1‑101 (MQ=255)                                                               tAATACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCATCTGATTAc                     >  2:1533094/1‑101 (MQ=255)                                                                aaTACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCATCTGATTAcc                    >  1:1402641/1‑101 (MQ=255)                                                                  tACCACAACCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCATCTGATTACCTg                  >  2:961505/1‑101 (MQ=255)                                                                        aaCCCTTAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCATCTGATTACCTGGCGGaa            <  1:66677/101‑1 (MQ=255)                                                                             ttAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCATCTGATTACCTGGCGGAAATTaa       >  2:1079165/1‑101 (MQ=255)                                                                              tAAGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCATCTGATTACCTGGCGGAAATTaaa      >  2:366915/1‑101 (MQ=255)                                                                               aaGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCATCTGATTACCTGGCGGAAATTAAAc     <  1:368663/101‑1 (MQ=255)                                                                                aGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCATCTGATTACCTGGCGGAAATTAAACt    <  1:813652/101‑1 (MQ=255)                                                                                aGTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCATCTGATTACCTGGCGGAAATTAAACt    >  1:1643634/1‑101 (MQ=255)                                                                                 gTTAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCATCTGATTACCTGGCGGAAATTAAACTa   <  2:1285075/101‑1 (MQ=255)                                                                                  ttAGTGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCATCTGATTACCTGGCGGAAATTAAACTaa  <  2:1207994/101‑1 (MQ=255)                                                                                      |                                                                                                 GCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGGAAACATCCCCATATACTGACGTACATGTTAATAGATGGCGTGAAGCACAGTCGTGTCATCTGATTACCTGGCGGAAATTAAACTAA  >  REL606/430750‑430931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑