| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 393,720 | G→A | R96H (CGC→CAC) | queA → | S‑adenosylmethionine:tRNA ribosyltransferase‑isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 393,720 | 0 | G | A | 95.6% | 140.2 / ‑0.7 | 45 | R96H (CGC→CAC) | queA | S‑adenosylmethionine:tRNA ribosyltransferase‑isomerase |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (20/23); total (21/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.37e-01 | |||||||||||
TGATCCCGGCGCGCCTGTTTGGGCGTAAAGCCAGCGGCGGCAAGATTGAAGTGCTGGTTGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCGCGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATGACGAAAGTATTAACGCAACAATGACCGCGCGCCACGGCGCACTGTTTG > REL606/393621‑393815 | tGATCCCGGCGCGCCTGTTTGGGCGTAAAGCCAGCGGCGGCAAGATTGAAGTGCTGGTTGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTcgc > 2:386552/1‑101 (MQ=255) gATCCCGGCGCGCCTGTTTGGGCGTAAAGCCAGCGGCGGCAAGATTGAAGTGCTGGTTGAACGGATGGTCGACGACAAACGCATTCTTGCGCATATTcacg > 2:1148246/1‑101 (MQ=255) cccGGCGCGCCTGTTTGGGCGTAAAGCCAGCGGCGGCAAGATTGAAGCGCTGGTTGAACGGATGGTCGACGAAAAACGCATTCTTGCGCATATTAACGCCt > 2:1205335/1‑101 (MQ=255) cGGCGCGCCTGTTTGGGCGTAAAGCCAGCGGCGGCAAGATTTGAGTGCGGGTTGACCGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCg < 2:1462565/101‑1 (MQ=255) ggCGCGCCTGTTTGGGCGTAAAGCCAGCGGCGGCAAGATTGAAGTGCTGGTTGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGa < 1:542715/101‑1 (MQ=255) gcgcCTGTTTGGGCGTAAAGCCAGCGGCGGCAAGATTGAAGTGCTGGTTGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAg > 1:920406/1‑101 (MQ=255) cTGTTTGGGCGTAAAGCCAGCGGCGGCAAGATTGAAGTGCTGGTTGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGcc < 2:1040452/101‑1 (MQ=255) gTTTGGGCGTAAAGCCAGCGGCGGCAAGATTGAAGTGCTGGTTGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCaa > 1:1707359/1‑101 (MQ=255) gTAAAGCCAGCGGCGGCAAGATTGAAGTGCTGGTTGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGgc > 1:1023580/1‑101 (MQ=255) tAAAGCCAGCGGCGGCAAGATTGAAGTGCTGGTTGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGgcg < 2:1481845/101‑1 (MQ=255) aGCCAGCGGCGGCAAGATTGAAGTGCTGGTTGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAg < 1:428126/101‑1 (MQ=255) aGCCAGCGGCGGCAAGATTGAAGTGCTGGTTGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAg > 1:1569104/1‑101 (MQ=255) cAGCGGCGGCAAGATTGAAGTGCTGGTTGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAAc < 1:253288/101‑1 (MQ=255) cAGCGGCGGCAAGATTGAAGTGCTGGTTGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCGCGAAAGCGCCAAAACCTGGCGCAGAAc < 2:264974/101‑1 (MQ=255) ggcggcAAGATTGAAGTGCTGGTTGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAActgct > 1:369526/1‑101 (MQ=255) aaGATTGAAGTGCTGGTTGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTggg < 2:1327666/101‑1 (MQ=255) aaGATTGAAGTGCTGGTTGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTggg > 1:821541/1‑101 (MQ=255) agtttGAAGTGCTGGTTGAACGGATGCTCGACGACAAACGCATTTTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGc > 2:1087328/4‑101 (MQ=255) gATTGAAGTGCTGGTTGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCg > 1:1402312/1‑101 (MQ=255) ttGAAGTGCTGGTTGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGAt < 2:829465/101‑1 (MQ=255) tGAAGTGCTGGTTGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCGCGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATg < 2:954443/101‑1 (MQ=255) gAAGTGCTGGTTGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATGa < 2:931411/101‑1 (MQ=255) aGTGCTGGTTGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATGACg < 2:58832/101‑1 (MQ=255) tGCTGGTTGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATGACGaa < 2:1279275/101‑1 (MQ=255) ggTTGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATGACGAAAgca > 1:1695012/1‑99 (MQ=255) ggTTGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATGACGAAAGTa > 2:291798/1‑101 (MQ=255) gAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATGACGAAAGTATTaa < 1:874881/101‑1 (MQ=255) aaCGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATGACGAAAGTATTAAc < 1:884951/101‑1 (MQ=255) ggATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATGACGAAAGTATTAACGca < 1:1651197/101‑1 (MQ=255) aTGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATGACGAAAGTATTAACGcaac < 2:254871/101‑1 (MQ=255) tGCTCGACGACAACCGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAGCCTGGCGCAGAACTGCTGCTGGGCGATGACGAAAGTATTAACGcaaca < 2:1023575/101‑1 (MQ=255) cTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATGACGAAAGTATTAACGCAACAAt < 2:1338891/101‑1 (MQ=255) cTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATGACGAAAGTATTAACGCAACAAt > 2:1298935/1‑101 (MQ=255) cgacgaCAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGGTGGGCGATGACGAAAGTATTAACGCAACAATGa < 2:835977/101‑1 (MQ=255) cgacgaCAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATGACGAAAGTATTAACGCAACAATGa < 1:1068714/101‑1 (MQ=255) cgacAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATGACGAAAGTATTAACGCAACAATGACcg < 2:868968/101‑1 (MQ=255) cAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATGACGAAAGTATTAACGCAACAATGACcgcgc > 1:955717/1‑101 (MQ=255) aaaCGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATGACGAAAGTATTAACGCAACAATGACcgcgcg > 1:1004544/1‑101 (MQ=255) aaaCGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATGACGAAAGTATTAACGCAACAATGACcgcgcg > 1:270991/1‑101 (MQ=255) aCGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATGACGAAAGTATTAACGCAACAATGACCGCGCGcc > 1:159179/1‑101 (MQ=255) cGCATTCTTGCGCATATTCGCGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATGACGAAAGTATTAACGCAACAATGACCGCGCGCCa > 2:881133/1‑101 (MQ=255) cGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATGACGAAAGTATTAACGCAACAATGAcactgactca < 2:396600/101‑10 (MQ=255) cGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATGACGAAAGTATTAACGCAACAATGACCGCGCGCCa > 2:355704/1‑101 (MQ=255) gCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATGACGAAAGTATTAACGCAACAATGACCGCGCGCCAc < 2:335516/101‑1 (MQ=255) ttGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATGACGAAAGTATTAACGCAACAATGACCGCGCGCCACGGCGCa > 2:469275/1‑101 (MQ=255) gcgcATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATGACGAAAGTATTAACGCAACAATGACCGCGCGCCACGGCGCACt > 2:779114/1‑101 (MQ=255) atatTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATGACGAAAGTATTAACGCAACAATGACCGCGCGCCACGGCGCACTGttt > 2:1141217/1‑101 (MQ=255) tatTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATGACGAAAGTATTAACGCAACAATGACCGCGCGCCACGGCGCACTGTTTg > 2:591977/1‑101 (MQ=255) tatTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATGACGAAAGTATTAACGCAACAATGACCGCGCGCCACGGCGCACTGTTTg > 1:139559/1‑101 (MQ=255) | TGATCCCGGCGCGCCTGTTTGGGCGTAAAGCCAGCGGCGGCAAGATTGAAGTGCTGGTTGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCGCGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATGACGAAAGTATTAACGCAACAATGACCGCGCGCCACGGCGCACTGTTTG > REL606/393621‑393815 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |