| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,409,316 | T→G | S41S (TCT→TCG) | yheS → | fused predicted transporter subunits of ABC superfamily: ATP‑binding components |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,409,316 | 0 | T | G | 98.3% | 228.2 / ‑4.3 | 59 | S41S (TCT→TCG) | yheS | fused predicted transporter subunits of ABC superfamily: ATP‑binding components |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base G (25/33); total (26/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.41e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01 | |||||||||||
TTCGTCGCGGCGTGCGCGTCCTGCTGGATAATGCCACCGCCACCATCAACCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCTACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACACCTTTCCGGGAAGCTGGCAACTGGCGTGGGTGAATCAGGAAACGCCG > REL606/3409219‑3409415 | tgcgtcgCGGCGTGCGCGTCCTGCTGGATAATGCCACCGCCACCATCAACCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGAcc < 1:39038/99‑1 (MQ=255) tcgtcgCGGCGTGCGCGTCCTGCTGGATAATGCCACCGCCACCATCAACCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGAccc < 2:299956/101‑1 (MQ=255) cgcgGCGTGCGCGTCCTGCTGGATAATGCCACCGCCACCATCAACCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCctgct > 2:1223494/1‑101 (MQ=255) gcggcgTGCGCGTCCTGCTGGATAATGCCACCGCCACCATCAACCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCctgctg < 1:233636/101‑1 (MQ=255) cgtgcgtgTCCTGCTGGATAATGCCACCGCCACCATCAACCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCAt < 1:1078503/101‑1 (MQ=255) cgTGCGCGTCCTGCTGGATAATGCCACCGCCACCATCAACCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCAt < 2:500182/101‑1 (MQ=255) cgcgTCCTGCTGGATAATGCCACCGCCACCATCAACCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCt < 1:628542/101‑1 (MQ=255) gTCCTGCTGGATAATGCCACCGCCACCATCAACCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGaa > 1:1037920/1‑101 (MQ=255) ctgGATAATGCCACCGCCACCATCAACCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGa > 2:123909/1‑101 (MQ=255) tgGATAATGCCACCGCCACCATCAACCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGaa < 2:1079873/101‑1 (MQ=255) tgGATAATGCCACCGCCACCATCAACCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGaa > 2:671700/1‑101 (MQ=255) tgGATAATGCCACCGCCACCATCAACCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGaa > 1:914104/1‑101 (MQ=255) ggATAATGCCACCGCCACCATCAACCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGaaa > 1:1568982/1‑101 (MQ=255) ggATAATGCCACCGCCACCATCAACCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGaaa < 1:1228823/101‑1 (MQ=255) ggATAATGCCACCGCCACCATCAACCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGaaa < 1:490984/101‑1 (MQ=255) gATAATGCCACCGCCACCATCAACCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAAt > 1:1250659/1‑101 (MQ=255) aTAATGCCACCGCCACCATCAACCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATc > 1:1296252/1‑101 (MQ=255) aTAATGCCACCGCCACCATCAACCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATc < 1:745493/101‑1 (MQ=255) aaTGCCACCGCCACCATCAACCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAg < 2:1719240/101‑1 (MQ=255) tGCCACCGCCACCATCAACCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAgcg < 1:1223499/101‑1 (MQ=255) gccaccgccaccATCAACCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAgcgc < 2:876285/101‑1 (MQ=255) ccgccaccATCAACCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGAc > 2:488243/1‑101 (MQ=255) gccaccATCAACCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGAcgg < 1:705128/101‑1 (MQ=255) ccaccaTCAACCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGAcggc > 2:209493/1‑101 (MQ=255) ccaTCAACCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACggcggc > 1:32790/1‑101 (MQ=255) caTCAACCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCa < 2:438376/101‑1 (MQ=255) aTCAACCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAg > 2:1182629/1‑101 (MQ=255) cAACCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCt > 2:1297146/1‑101 (MQ=255) aCCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTac < 1:517863/101‑1 (MQ=255) ccGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACAcc < 2:1296246/101‑1 (MQ=255) gggCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACACCtt > 1:1113749/1‑101 (MQ=255) cAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACACCTTTcc > 2:877217/1‑101 (MQ=255) aGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACACCTTTCCg < 1:1006437/101‑1 (MQ=255) gAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACACCTTTCCgg < 1:919661/101‑1 (MQ=255) gAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACACCTTTCCgg < 2:1032067/101‑1 (MQ=255) aaaGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACACCTTTCCggg > 2:742617/1‑101 (MQ=255) gTCGGCCTGGTGGGTAAAAACGGCTGTGGGAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACACCTTTCCGGGAAg > 2:844983/1‑101 (MQ=255) cGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACACCTTTCCGGGAAGCt < 2:999472/101‑1 (MQ=255) ggtggGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACACCTTTCCGGGAAGCTGGCAAc < 2:32790/101‑1 (MQ=255) gtggGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACCCCTTTCCGGGAAGCTGGCAACt < 1:488248/101‑1 (MQ=255) tggGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACACCTTTCCGGGAAGCTGGCAACTg > 1:676067/1‑101 (MQ=255) tggGTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACACCTTTCCGGGAAGCTGGCAACTg > 1:76587/1‑101 (MQ=255) gggTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACACCTTTCCGGGAAGCTGGCAACTgg < 1:623731/101‑1 (MQ=255) ggTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACACCTTTCCGGGAAGCTGGCAACTGGc < 1:1080947/101‑1 (MQ=255) gTAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACACCTTTCCGGGAAGCTGGCAACTGGCg > 2:671062/1‑101 (MQ=255) tAAAAACGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACACCTTTCCGGGAAGCTGGCAACTGGCGt > 2:928962/1‑101 (MQ=255) aaaCGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACACCTTTCCGGGAAGCTGGCAACTGGCGTggg > 1:714846/1‑101 (MQ=255) aCGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACACCTTTCCGGGAAGCTGGCAACTGGCGTGGGTg < 1:644147/101‑1 (MQ=255) cGGCTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACACCTTTCCGGGAAGCTGGCAACTGGCGTGGGTGa > 1:104326/1‑101 (MQ=255) cTGTGGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACACCTTTCCGGGAAGCTGGCAACTGGCGTGGGTGAATc < 1:671705/101‑1 (MQ=255) tgtgGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACACCTTTCCGGGAAGCTGGCAACTGGCGTGGGTGAATCa > 1:770549/1‑101 (MQ=255) tgGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACACCTTTCCGGGAAGCTGGCAACTGGCGTGGGTGAATCAgg > 2:1452415/1‑101 (MQ=255) tgGTAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACACCTTTCCGGGAAGCTGGCAACTGGCGTGGGTGAATCAgg < 2:1568976/101‑1 (MQ=255) gTAAATCGACCCTGCTGGCANTNCTGAAAAATGAAATNAGCGCCGACGGCGGCAGCTACACCTTTCCGGGAAGCTGGCAACTGGCGTGGGTGAATCAGGaa < 2:226626/101‑1 (MQ=255) tAAATCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACACCTTTCCGGGAAGCTGGCAACTGGCGTGGGTGAATCAGGaaa < 2:504135/101‑1 (MQ=255) aaaTCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACACCTTTCCGGGAAGCTGGCAACTGGCGTGGGTGAATCAGGAAAc < 1:206030/101‑1 (MQ=255) aaTCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACACCTTTCCGGGAAGCTGGCAACTGGCGTGGGTGAATCAGGAAACg < 1:1206535/101‑1 (MQ=255) aTCGACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACACCTTTCCGGGAAGCTGGCAACTGGCGTGGGTGAATCAGGAAACGc < 2:676062/101‑1 (MQ=255) cTACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACACCTTTCCGGGAAGCTGGCAACTGGCGTGGGTGAATCAGGAAACGCCg > 2:82202/1‑101 (MQ=255) | TTCGTCGCGGCGTGCGCGTCCTGCTGGATAATGCCACCGCCACCATCAACCCCGGGCAGAAAGTCGGCCTGGTGGGTAAAAACGGCTGTGGTAAATCTACCCTGCTGGCATTGCTGAAAAATGAAATCAGCGCCGACGGCGGCAGCTACACCTTTCCGGGAAGCTGGCAACTGGCGTGGGTGAATCAGGAAACGCCG > REL606/3409219‑3409415 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |