Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,093,020	T→G	F74L (TTT→TTG)	yqhH →	predicted outer membrane lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,093,020	0	T	G	100.0%	350.2 / NA	87	F74L (TTT→TTG)	yqhH	predicted outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (48/39);  total (48/39)

CGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTTGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGATATTTTTTAACGTCCAACCGCCGCT  >  REL606/3092922‑3093117                                                                                                   |                                                                                                  cGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGa                                                                                                 >  2:1266443/1‑101 (MQ=255) cGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGa                                                                                                 >  2:693534/1‑101 (MQ=255)  ggCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGAt                                                                                                <  2:1042416/101‑1 (MQ=255)   gCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGAtt                                                                                               <  1:1025058/101‑1 (MQ=255)   gCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGAtt                                                                                               <  2:144396/101‑1 (MQ=255)    cTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTg                                                                                              >  2:1321041/1‑101 (MQ=255)    cTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTg                                                                                              <  2:659489/101‑1 (MQ=255)    cTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTg                                                                                              <  2:1454449/101‑1 (MQ=255)     ttGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGc                                                                                             <  1:894864/101‑1 (MQ=255)        aGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTg                                                                                          <  2:897865/101‑1 (MQ=255)        aGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTg                                                                                          >  2:1687833/1‑101 (MQ=255)        aGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTg                                                                                          >  1:957847/1‑101 (MQ=255)          cAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTgcg                                                                                        >  2:717899/1‑101 (MQ=255)           aGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTgcgc                                                                                       >  2:206571/1‑101 (MQ=255)              atatGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGANTNNNTGGATTGCCTGCgctgc                                                                                    >  2:300264/1‑101 (MQ=255)              atatGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCgctgc                                                                                    >  2:1198482/1‑101 (MQ=255)               tatGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCgctgct                                                                                   <  1:870655/101‑1 (MQ=255)                  gAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGc                                                                                >  2:172704/1‑101 (MQ=255)                  gAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGc                                                                                <  1:578992/101‑1 (MQ=255)                  gAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGc                                                                                <  1:1610170/101‑1 (MQ=255)                      gCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTAt                                                                            <  2:943237/101‑1 (MQ=255)                            cGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGc                                                                      <  2:887628/101‑1 (MQ=255)                             gCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCa                                                                     <  2:268322/101‑1 (MQ=255)                              cccACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAg                                                                    <  1:324339/101‑1 (MQ=255)                               ccACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGa                                                                   >  2:1469846/1‑101 (MQ=255)                                      tCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATaa                                                            <  2:703841/101‑1 (MQ=255)                                        tATGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATaaaa                                                          >  1:689883/1‑101 (MQ=255)                                         aTGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATaaaaa                                                         <  2:112983/101‑1 (MQ=255)                                          tgctgcCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATaaaaaa                                                        <  1:969579/101‑1 (MQ=255)                                          tgctgcCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATaaaaaa                                                        >  1:829570/1‑101 (MQ=255)                                            ctgcCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAAt                                                      >  2:1679237/1‑101 (MQ=255)                                            ctgcCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAAt                                                      >  2:1126933/1‑101 (MQ=255)                                            ctgcCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAAt                                                      <  2:827807/101‑1 (MQ=255)                                             tgcCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATc                                                     >  1:928422/1‑101 (MQ=255)                                             tgcCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATc                                                     <  2:1503858/101‑1 (MQ=255)                                             tgcCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATc                                                     >  1:1304760/1‑101 (MQ=255)                                             tgcCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATc                                                     >  2:1288277/1‑101 (MQ=255)                                              gcCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACTCAGAATGATAAAAAAATcc                                                    <  2:1059422/101‑1 (MQ=255)                                               ccAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATccc                                                   <  1:1687839/101‑1 (MQ=255)                                               ccAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATccc                                                   <  2:957842/101‑1 (MQ=255)                                               ccAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATccc                                                   >  1:1402140/1‑101 (MQ=255)                                                 aaaTCCGAAGCTAACAGAGCCAATTCGCGTCTTGATGCTCAGGTCTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAAAccccc                                                 <  2:269202/101‑2 (MQ=255)                                                 aaaTCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCg                                                 <  2:298910/101‑1 (MQ=255)                                                 aaaTCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCg                                                 <  2:1480725/101‑1 (MQ=255)                                                  aaTCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCgg                                                <  2:928417/101‑1 (MQ=255)                                                    tCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTa                                              >  2:1205162/1‑101 (MQ=255)                                                        aaGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCgt                                          >  2:540886/1‑101 (MQ=255)                                                           cTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTc                                       >  2:130622/1‑101 (MQ=255)                                                           cTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTc                                       >  1:1028703/1‑101 (MQ=255)                                                            tAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCa                                      <  1:330287/101‑1 (MQ=255)                                                            tAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCa                                      >  1:842602/1‑101 (MQ=255)                                                             aaCAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAg                                     >  2:1545726/1‑101 (MQ=255)                                                              aCAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGt                                    <  2:1459289/101‑1 (MQ=255)                                                               cAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGtt                                   >  1:431301/1‑101 (MQ=255)                                                                agagCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTg                                  <  2:1265116/101‑1 (MQ=255)                                                                agagCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTg                                  <  2:185314/101‑1 (MQ=255)                                                                agagCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTg                                  >  2:696737/1‑101 (MQ=255)                                                                  agCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGcc                                >  2:581876/1‑101 (MQ=255)                                                                  agCCAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGcc                                >  1:775391/1‑101 (MQ=255)                                                                     cAATACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCggg                             >  1:1182867/1‑101 (MQ=255)                                                                      aaTACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCgggg                            >  1:346085/1‑101 (MQ=255)                                                                      aaTACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCgggg                            >  1:1226943/1‑101 (MQ=255)                                                                        tACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGat                          <  2:829565/101‑1 (MQ=255)                                                                        tACGCGTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGat                          <  2:235507/101‑1 (MQ=255)                                                                          cgcgTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGatat                        >  2:263744/1‑101 (MQ=255)                                                                             gTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGATAtttt                     >  1:1336075/1‑101 (MQ=255)                                                                             gTCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGATAtttt                     >  2:1623961/1‑101 (MQ=255)                                                                              tCTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGATAttttt                    >  2:600247/1‑101 (MQ=255)                                                                               cTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGATAtttttt                   >  1:666563/1‑101 (MQ=255)                                                                               cTTGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGATAtttttt                   <  1:1205167/101‑1 (MQ=255)                                                                                ttGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGATATTTTTTa                  >  2:540113/1‑101 (MQ=255)                                                                                 tGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGATATTTTTTaa                 <  1:19073/101‑1 (MQ=255)                                                                                 tGATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGATATTTTTTaa                 <  1:1604934/101‑1 (MQ=255)                                                                                  gATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGATATTTTTTAAc                <  1:1623967/101‑1 (MQ=255)                                                                                  gATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGATATTTTTTAAc                <  1:576096/101‑1 (MQ=255)                                                                                  gATGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGATATTTTTTAAc                >  2:1669009/1‑101 (MQ=255)                                                                                   aTGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGATATTTTTTAACg               >  1:21469/1‑101 (MQ=255)                                                                                   aTGCTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGATATTTTTTAACg               >  1:1146105/1‑101 (MQ=255)                                                                                      cTCAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGATATTTTTTAACGTcc            <  1:726114/101‑1 (MQ=255)                                                                                        cAGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGATATTTTTTAACGTCCaa          >  1:1155083/1‑101 (MQ=255)                                                                                         aGGACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGATATTTTTTAACGTCCAAc         >  1:1396071/1‑101 (MQ=255)                                                                                           gACTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGATATTTTTTAACGTCCAAccg       >  1:1701963/1‑101 (MQ=255)                                                                                             cTATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGATATTTTTTAACGTCCAAccgcc     <  2:349830/101‑1 (MQ=255)                                                                                              tATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGATATTTTTTAACGTCCAAccgccg    <  2:974429/101‑1 (MQ=255)                                                                                              tATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGATATTTTTTAACGTCCAAccgccg    >  2:919160/1‑101 (MQ=255)                                                                                              tATTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGATATTTTTTAACGTCCAAccgccg    >  1:1623149/1‑101 (MQ=255)                                                                                               aTTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGATATTTTTTAACGTCCAACcgccgc   >  2:1048091/1‑101 (MQ=255)                                                                                               aTTTGGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGATATTTTTTAACGTCCAACcgccgc   >  2:1459448/1‑101 (MQ=255)                                                                                                tttgGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGATATTTTTTAACGTCCAACCGCCGCt  >  2:1564400/1‑101 (MQ=255)                                                                                                   |                                                                                                  CGGCTTGAGCAGGATATGAAAGCACTACGCCCACAAATCTATGCTGCCAAATCCGAAGCTAACAGAGCCAATACGCGTCTTGATGCTCAGGACTATTTTGATTGCCTGCGCTGCTTGCGTATGTACGCAGAATGATAAAAAAATCCCCGGTAGCGTGTCAGTTGCCGGGGATATTTTTTAACGTCCAACCGCCGCT  >  REL606/3092922‑3093117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑