| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,847,052 | A→G | V10A (GTT→GCT) | recD ← | exonuclease V (RecBCD complex), alpha chain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,847,052 | 0 | A | G | 98.5% | 267.7 / NA | 67 | V10A (GTT→GCT) | recD | exonuclease V (RecBCD complex), alpha chain |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (25/41); minor base T (1/0); total (26/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTAACAGGGCTGCCGCGAGGGTGACGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAACTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGTCAATCAACCCTGCATTGGGTC > REL606/2846954‑2847147 | ttAACAGGGCTGCCGCGAGGGTGACGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCt < 2:77559/101‑1 (MQ=255) aCAGGGCTGCCGCGAGGGTGACGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTcc < 1:436651/101‑1 (MQ=255) gggCTGCCGCGAGGGTGACGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGt < 1:37977/101‑1 (MQ=255) cTGCCGCGAGGGTGACGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAAt < 2:181800/101‑1 (MQ=255) cTGCCGCGAGGGTGACGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAAt < 2:986339/101‑1 (MQ=255) tGCCGCGAGGGTGACGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAAtt > 2:838436/1‑101 (MQ=255) ccGCGAGGGTGACGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTCATTGc < 2:1424383/101‑1 (MQ=255) ccGCGAGGGTGACGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGc < 2:1499344/101‑1 (MQ=255) cgAGGGTGACGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCttt < 1:125436/101‑1 (MQ=255) gAGGGTGACGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCtttt < 1:785483/101‑1 (MQ=255) ggTGACGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCa < 1:1310648/101‑1 (MQ=255) ggTGACGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCa > 2:765381/1‑101 (MQ=255) ggTGACGGCAGGATGTTCATCCCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCa < 2:243131/101‑1 (MQ=255) gTGACGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCaa > 2:169160/1‑101 (MQ=255) aCGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAAttt < 2:1104471/101‑1 (MQ=255) ggCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCa > 2:598583/1‑101 (MQ=255) cAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCAtt < 2:1374089/101‑1 (MQ=255) aGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTa < 2:1551339/101‑1 (MQ=255) aGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTa < 1:185043/101‑1 (MQ=255) ggATGTTCATCTCCCGCCACGGTTAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTAc < 1:805110/101‑1 (MQ=255) ggATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTAc < 1:866239/101‑1 (MQ=255) ggATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTAc < 1:693419/101‑1 (MQ=255) ggATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTAc < 2:8569/101‑1 (MQ=255) tGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGGTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCAttcactc > 2:334314/1‑96 (MQ=255) tttCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGcct < 2:40608/100‑1 (MQ=255) gTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGcct < 1:405572/101‑1 (MQ=255) gTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGcct > 2:879111/1‑101 (MQ=255) gTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGcct < 1:785348/101‑1 (MQ=255) tCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGcctcc < 2:514349/101‑1 (MQ=255) tctcCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCtcctcc < 1:1024614/101‑1 (MQ=255) cccGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAgg > 1:432221/1‑101 (MQ=255) ccACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCa < 2:999873/101‑1 (MQ=255) ccACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCa > 2:629017/1‑101 (MQ=255) cACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCAt < 1:838441/101‑1 (MQ=255) cACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTCCATTACGCCTCCTCCAGGGTCAt > 1:247862/1‑101 (MQ=255) aCGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATa < 1:1463760/101‑1 (MQ=255) cGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATAc < 2:1330029/101‑1 (MQ=255) ggTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATAcc < 1:60357/101‑1 (MQ=255) cAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGc > 1:885653/1‑101 (MQ=255) aGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCa < 2:318288/101‑1 (MQ=255) gCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAAc < 1:1271183/101‑1 (MQ=255) aaaTTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACAt > 1:789861/1‑101 (MQ=255) aaaTTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACAt > 1:876577/1‑101 (MQ=255) aTTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACAtct < 1:307316/101‑1 (MQ=255) ttGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACAtctc > 2:45758/1‑101 (MQ=255) tGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTca < 2:490400/101‑1 (MQ=255) gCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTcat < 1:1340482/101‑1 (MQ=255) acaTCCAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTcatcc < 2:1166002/101‑1 (MQ=255) ccAGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCa > 1:639802/1‑101 (MQ=255) aGCGGGCGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAgg > 1:1499704/1‑101 (MQ=255) cGGGCGTAGCTGTTTGTGCTCCACATCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGtc > 2:811823/1‑101 (MQ=255) cGTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGtcaatc < 1:598588/101‑1 (MQ=255) gTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGtcaatca > 1:352185/1‑101 (MQ=255) gTAGCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGtcaatca > 2:1256572/1‑101 (MQ=255) gCTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGTCAATCAAcc > 2:863776/1‑101 (MQ=255) cTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGTCAATCAAccc > 1:1317875/1‑101 (MQ=255) cTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGTCAATCAAccc > 2:1402302/1‑101 (MQ=255) cTGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGTCAATCAAccc < 1:747432/101‑1 (MQ=255) tGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGTCAATCAACCCt < 1:832836/101‑1 (MQ=255) tGTTTGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGTCAATCAACCCt > 2:1060979/1‑101 (MQ=255) ttGTGCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGTCAATCAACCCTGCa < 1:169160/101‑1 (MQ=255) tgtgCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGTCAATCAACCCTGCAt > 1:1256329/1‑101 (MQ=255) cttgCTCCACAGCCTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGTCAATCAACCCTGCAt < 2:1647062/99‑1 (MQ=255) tgCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGTCAATCAACCCTGCATTg < 1:961317/101‑1 (MQ=255) gCTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGTCAATCAACCCTGCATTgg < 1:663640/101‑1 (MQ=255) cTCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGTCAATCAACCCTGCATTggg > 1:738786/1‑101 (MQ=255) tCCACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGTCAATCAACCCTGCATTGGGt > 1:1287712/1‑101 (MQ=255) ccACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGTCAATCAACCCTGCATTGGGTc > 1:587676/1‑101 (MQ=255) ccACAGCTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGTCAATCAACCCTGCATTGGGTc > 1:371208/1‑101 (MQ=255) | TTAACAGGGCTGCCGCGAGGGTGACGGCAGGATGTTCATCTCCCGCCACGGTCAGGGCAAATTGCACATCCAGCGGGCGTAGCTGTTTGTGCTCCACAACTTCCAGTAATTGCTTTTGCAATTTCATTACGCCTCCTCCAGGGTCATACCGGCAAACATCTCATCCATCAGGTCAATCAACCCTGCATTGGGTC > REL606/2846954‑2847147 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |