Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | REL606 | 1,879,026 | T→A | D380V (GAT→GTT) | yoaE ← | fused predicted membrane protein/conserved protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | REL606 | 1,879,026 | 0 | T | A | 98.2% | 195.4 / ‑4.0 | 54 | D380V (GAT→GTT) | yoaE | fused predicted membrane protein/conserved protein |
Reads supporting (aligned to +/- strand): ref base T (0/1); new base A (24/29); total (24/30) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 |
AGCGGCGTGACCAAGCCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGATCGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACG > REL606/1878930‑1879120 | aGCGGCGTGACCAAGCCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAg < 1:176182/101‑1 (MQ=255) aGCGGCGTGACCAAGCCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAg > 1:329498/1‑101 (MQ=255) aGCGGCGTGACCAAGCCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAg < 2:474231/101‑1 (MQ=255) aGCGGCGTGACCAAGCCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAg < 2:1069125/101‑1 (MQ=255) cggcgTGACCAAGCCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGTCCAGCAGGTTGATCGGAACGAggg > 1:378749/1‑101 (MQ=255) ggcgTGACCAAGCCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGt < 2:893031/101‑1 (MQ=255) gTGACCAAGCCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTc > 2:612130/1‑101 (MQ=255) tGACCAAGCCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTcc > 1:601039/1‑101 (MQ=255) ccAAGCCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCggg > 1:388515/1‑101 (MQ=255) aGCCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACg < 2:1172971/101‑1 (MQ=255) ccTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGAt < 2:685944/101‑1 (MQ=255) ccTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGAt > 2:1416339/1‑101 (MQ=255) cTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGATCGAGGGTTTCCGGGACGATa < 2:1348621/101‑1 (MQ=255) cTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATa < 2:1149201/101‑1 (MQ=255) tGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAAt < 1:1067540/101‑1 (MQ=255) gTACCACACCAAACTCGTTGGTCACGATAGCAAAGCTCCAGCGAGCGCGACGCAGCACGCCAAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATc < 1:957654/101‑1 (MQ=255) gTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATc < 2:715384/101‑1 (MQ=255) ccACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGcc < 1:590622/101‑1 (MQ=255) cacaCCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCg < 1:612135/101‑1 (MQ=255) acacCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCgg > 2:515247/1‑101 (MQ=255) cacCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCGga > 2:817910/1‑101 (MQ=255) cAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCGGAGAc > 1:406331/1‑101 (MQ=255) cAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCGGAGAc > 2:1588594/1‑101 (MQ=255) gTTGGTCACGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAg < 1:686464/101‑1 (MQ=255) ttGGTCACGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGc < 2:564038/101‑1 (MQ=255) tGGTCACGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCa < 2:378744/101‑1 (MQ=255) tGGTCACGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCa > 2:813076/1‑101 (MQ=255) tGGTCACGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCa > 2:396905/1‑101 (MQ=255) gTCACGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAAt < 1:503613/101‑1 (MQ=255) gTCACGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAAt > 1:265873/1‑101 (MQ=255) cACGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATcg > 1:103774/1‑101 (MQ=255) cGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATcgcc > 2:974655/1‑101 (MQ=255) cGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATcgcc > 2:272062/1‑101 (MQ=255) aCAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCcac < 1:1560145/101‑1 (MQ=255) aGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCa < 1:1290397/101‑1 (MQ=255) aGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGAGCGGAACGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCa < 1:760044/101‑1 (MQ=255) cTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAAc < 2:191528/101‑1 (MQ=255) tCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACg > 2:1143311/1‑101 (MQ=255) cccGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGc < 1:515252/101‑1 (MQ=255) cccGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGc < 2:223128/101‑1 (MQ=255) cgcgAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGccc < 1:1118709/101‑1 (MQ=255) cgAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGCCctc < 1:731494/101‑1 (MQ=255) aGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGCCCTCtt < 2:853742/101‑1 (MQ=255) aGCGCGACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGCCCTCtt < 1:39387/101‑1 (MQ=255) cgcgACGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGCCCTCTTcc < 1:1233475/101‑1 (MQ=255) aCGCAGCACGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGCCCTCTTCCAgcg > 1:986164/1‑101 (MQ=255) agcaCGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGCCCTCTTCCAGCGccac < 1:679011/101‑1 (MQ=255) gcaCGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGCCCTCTTCCAGCGccacc > 1:890772/1‑101 (MQ=255) aCGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGCCCTCTTCCAGCGCCACcag > 2:1720005/1‑101 (MQ=255) aCGCCCAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGCCCTCTTCCAGCGCCACcag > 1:1562220/1‑101 (MQ=255) cccAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGCCCTCTTCCAGCGCCACcagcag > 2:421635/1‑101 (MQ=255) ccAGCAGGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGCCCTCTTCCAGCGCCACCAGCAGt < 2:329498/101‑1 (MQ=255) cagcagGTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGCCCTCTTCCAGCGCCACcagcagct > 1:632567/1‑99 (MQ=255) agcagGTTGATCGGAACGAGGGTTTCCGGGACGATAATCCCTGGGGAGGCCGTAGCAATCGCAGCCACATCAACACCCTCTTCAGCCGCTACCAGCATTTc > 2:500838/1‑101 (MQ=255) ggTTGATCGGAACGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGCCCTCTTCCAGCGCCACCAGCAGTTCTTTa > 1:809874/1‑101 (MQ=255) gatcggaacgAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCAc < 1:500389/101‑1 (MQ=255) atcggaacgAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACg < 2:1332604/101‑1 (MQ=255) | AGCGGCGTGACCAAGCCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCGCGACGCAGCACGCCCAGCAGGTTGATCGGATCGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACGCCCTCTTCCAGCGCCACCAGCAGTTCTTTAGCACG > REL606/1878930‑1879120 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |