Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	761,693	T→G	noncoding (47/76 nt)	valT →	tRNA‑Val

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	761,693	0	T	G	100.0%	253.1 / NA	75	noncoding (47/76 nt)	valT	tRNA‑Val
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (40/35);  total (40/35)

TCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCC  >  REL606/761595‑761786                                                                                                   |                                                                                              tCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGcg                                                                                             <  1:386029/101‑1 (MQ=255) tCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGcg                                                                                             >  1:955410/1‑101 (MQ=255)    tGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGGAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGgcggcg                                                                                          >  1:457910/1‑101 (MQ=255)        cGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTc                                                                                      <  2:627638/101‑1 (MQ=255)           cccACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGAt                                                                                   <  2:1404324/101‑1 (MQ=255)             caccacTAACATAGTTAGTTGTAGTATCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGCTcc                                                                                 >  2:1565863/1‑101 (MQ=255)              accacTAACATAGTTAGTTGTAGTATCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATccc                                                                                >  1:778435/1‑101 (MQ=255)               ccacTAACATAGTTAGTTGTAGTATCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCg                                                                               >  1:934970/1‑101 (MQ=255)                 acTAACATAGTTAGTTGTAGTATCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGtc                                                                             >  1:145939/1‑101 (MQ=255)                 acTAACATAGTTAGTTGTAGTATCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGtc                                                                             <  2:426987/101‑1 (MQ=255)                 acTAACATAGTTAGTTGTAGTATCCAGCGTAGTATTGGGTGATTAGCCCAGCTGGGAGAGCACCTCCCTCACAAGGAGGGGGGCGGCGGTTCGATCCCGtc                                                                             >  1:811907/1‑101 (MQ=255)                  cTAACATAGTTAGTTGTAGTATCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGtca                                                                            >  2:935131/1‑101 (MQ=255)                   tAACATAGTTAGTTGTAGTATCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGtcat                                                                           >  1:1006438/1‑101 (MQ=255)                   tAACATAGTTAGTTGTAGTATCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGtcat                                                                           >  2:200493/1‑101 (MQ=255)                   tAACATAGTTAGTTGTAGTATCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGtcat                                                                           <  2:139727/101‑1 (MQ=255)                    aaCATAGTTAGTTGTAGTATCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGtcatc                                                                          >  2:1069739/1‑101 (MQ=255)                     aCATAGTTAGTTGTAGTATCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGtcatca                                                                         >  1:1347298/1‑101 (MQ=255)                      cATAGTTAGTTGTAGTATCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATcac                                                                        >  1:316484/1‑101 (MQ=255)                      cATAGTTAGTTGTAGTATCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATcac                                                                        >  2:1162907/1‑101 (MQ=255)                      cATAGTTAGTTGTAGTATCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATcac                                                                        <  1:1526695/101‑1 (MQ=255)                        tagttagtTGTAGTATCCAGCGTAGTATTGGGGGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATTCCGtcatcatca                                                                      >  2:731312/1‑98 (MQ=14)                          gttagttGTAGTATCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATcacacac                                                                    >  1:1341151/1‑101 (MQ=255)                                tGTAGTATCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCgg                                                              <  1:594778/101‑1 (MQ=255)                                tGTAGTATCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCgg                                                              >  1:155544/1‑101 (MQ=255)                                 gtagtaTCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCggg                                                             <  1:217244/101‑1 (MQ=255)                                 gtagtaTCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCggg                                                             >  1:881435/1‑101 (MQ=255)                                 gtagtaTCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCggg                                                             <  1:901965/101‑1 (MQ=255)                                    gtaTCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCg                                                          >  2:716327/1‑101 (MQ=255)                                     taTCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTcct                                                         >  2:1327995/1‑99 (MQ=255)                                      aTCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGtt                                                        >  1:627438/1‑101 (MQ=255)                                       tCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTTCATTACACGGAGGCGGCCGGGGGTTCCATCCCGTCACCACCCACCGCCGTGTCgatc                                                       <  2:1545663/101‑4 (MQ=14)                                       tCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTa                                                       >  1:123075/1‑101 (MQ=255)                                       tCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTa                                                       <  1:731317/101‑1 (MQ=255)                                        ccAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTTCAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAg                                                      <  2:998907/101‑1 (MQ=255)                                          aGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCt                                                    <  1:694451/101‑1 (MQ=255)                                            cGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCa                                                  <  1:970847/101‑1 (MQ=255)                                             gtagtaTTGGGTGATTAGCTCAGCTGGGAGGGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCCTCACCCACCACCGGGTCGTTAGCTCAg                                                 >  1:1678731/1‑101 (MQ=14)                                                gtaTTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTg                                              <  2:88335/101‑1 (MQ=255)                                                  aTTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGt                                            >  1:1217392/1‑101 (MQ=255)                                                  aTTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGt                                            >  2:424780/1‑101 (MQ=255)                                                    tGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTag                                          <  1:651431/101‑1 (MQ=25)                                                     tggTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTaga                                         <  2:1250564/100‑1 (MQ=25)                                                     gggTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTaga                                         <  2:356838/101‑1 (MQ=25)                                                       gTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGc                                       >  1:1061726/1‑101 (MQ=25)                                                         gATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAg                                     >  2:790989/1‑101 (MQ=25)                                                         gATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAg                                     >  1:340255/1‑101 (MQ=25)                                                         gATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCACGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAg                                     >  1:961436/1‑101 (MQ=255)                                                          aTTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGt                                    >  2:1493199/1‑101 (MQ=25)                                                           ttAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGtt                                   >  1:511310/1‑101 (MQ=25)                                                           ttAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGtt                                   <  1:283215/101‑1 (MQ=25)                                                               cTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACt                               >  1:1591850/1‑101 (MQ=25)                                                               cTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACt                               >  2:931206/1‑101 (MQ=25)                                                                 cAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACttt                             >  2:1213565/1‑101 (MQ=25)                                                                   gCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTa                           <  1:71487/101‑1 (MQ=25)                                                                    cTGGGAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTaa                          <  1:1649303/101‑1 (MQ=25)                                                                      gggAGAGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATc                        <  2:388933/101‑1 (MQ=25)                                                                        gagaGCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCat                      >  2:1393800/1‑100 (MQ=25)                                                                         agagCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAAt                     <  1:1285348/101‑1 (MQ=25)                                                                          gagCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAAtt                    <  2:611960/101‑1 (MQ=25)                                                                           agCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTg                   <  2:961431/101‑1 (MQ=25)                                                                           agCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTg                   >  2:862985/1‑101 (MQ=25)                                                                            gCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTgg                  <  1:200493/101‑1 (MQ=25)                                                                            gCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTgg                  <  2:955405/101‑1 (MQ=25)                                                                            gCACCTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTgg                  <  2:152979/101‑1 (MQ=25)                                                                               ccTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCg               <  2:1006433/101‑1 (MQ=25)                                                                               ccTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCg               >  2:666869/1‑101 (MQ=25)                                                                                cTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGc              >  2:222179/1‑101 (MQ=25)                                                                                  cccTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAg            >  2:191538/1‑101 (MQ=25)                                                                                  cccTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAg            <  1:666874/101‑1 (MQ=25)                                                                                   ccTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAgg           <  1:677991/101‑1 (MQ=25)                                                                                   ccTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAgg           <  2:145939/101‑1 (MQ=25)                                                                                    cTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGt          <  1:1579966/101‑1 (MQ=25)                                                                                    cTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGt          <  2:881430/101‑1 (MQ=25)                                                                                    cTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCCTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGt          >  2:1178138/1‑101 (MQ=255)                                                                                      tACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCTCAGGTTc        >  1:754157/1‑101 (MQ=255)                                                                                      tACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTc        <  2:1217387/101‑1 (MQ=25)                                                                                       aCAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCg       >  2:1292770/1‑101 (MQ=25)                                                                                        cAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGGCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGa      >  2:876711/1‑101 (MQ=255)                                                                                            gaggGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATcc  <  1:935136/101‑1 (MQ=25)                                                                                                   |                                                                                              TCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCC  >  REL606/761595‑761786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑