Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	REL606	3,766,595	IS150 (+) +3 bp	coding (1111‑1113/1206 nt)	gltS ←	glutamate transporter

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	3766595 =	0 (0.000)	57 (0.890)	47/198	0.4	100%	coding (1113/1206 nt)	gltS	glutamate transporter
	?	REL606	= 3894996	NA (NA)					noncoding (1443/1443 nt)	IS150	repeat region
*	?	REL606	= 3766597	0 (0.000)	85 (1.320)	67/200	0.0	100%	coding (1111/1206 nt)	gltS	glutamate transporter
	?	REL606	3893554 =	NA (NA)					noncoding (1/1443 nt)	IS150	repeat region

AGAATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGACGCCTCC  >  REL606/3894899‑3895005                                                                                                  |          agaaTTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGAc        <  2:1677981/101‑1 (MQ=32) agaaTTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGAc        <  2:340227/101‑1 (MQ=32)       aGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAggctgtact  <  2:115273/101‑9 (MQ=2)                                                                                                  |          AGAATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGACGCCTCC  >  REL606/3894899‑3895005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.