Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	REL606	3,981,782	IS150 (–) +3 bp	coding (249‑251/1224 nt)	ECB_03710 ←	putative permease

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	3893554 =	NA (NA)	113 (1.760)	73/200	0.0	98.2%	noncoding (1/1443 nt)	IS150	repeat region
	?	REL606	3981782 =	2 (0.030)					coding (251/1224 nt)	ECB_03710	putative permease
*	?	REL606	= 3894996	NA (NA)	120 (1.880)	79/198	0.0	98.3%	noncoding (1443/1443 nt)	IS150	repeat region
	?	REL606	= 3981784	2 (0.030)					coding (249/1224 nt)	ECB_03710	putative permease

AGAATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGACGCCTCC  >  REL606/3894899‑3895005                                                                                                  |          agaaTTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGAc        <  2:1677981/101‑1 (MQ=32) agaaTTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGAc        <  2:340227/101‑1 (MQ=32)       aGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAggctgtact  <  2:115273/101‑9 (MQ=2)                                                                                                  |          AGAATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACAGACGCCTCC  >  REL606/3894899‑3895005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.