Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	REL606	2,322,340	IS186 (+) +6 bp	coding (131‑136/963 nt)	menC ←	O‑succinylbenzoate synthase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	2322340 =	0 (0.000)	41 (0.660)	35/194	1.0	100%	coding (136/963 nt)	menC	O‑succinylbenzoate synthase
	?	REL606	= 2774196	NA (NA)					noncoding (1343/1343 nt)	IS186	repeat region
*	?	REL606	= 2322345	0 (0.000)	53 (0.850)	40/194	0.6	100%	coding (131/963 nt)	menC	O‑succinylbenzoate synthase
	?	REL606	2772854 =	NA (NA)					noncoding (1/1343 nt)	IS186	repeat region

TCGCAATCGCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCTccc‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/2322249‑2322345 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCAT  >  REL606/2772854‑2772952                                                                                                                                                                                                      TCGCAATCGCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCA                                                                                                 <  1:1607420/101‑1  CGCAATCGCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCAT                                                                                                <  1:231821/101‑1  CGCAATCGCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCAT                                                                                                <  2:1110955/101‑1     AATCGCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCATAAG                                                                                             >  1:72001/1‑101        CGCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCATAAGCGC                                                                                          <  1:1489859/101‑1         GCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCATAAGCGCT                                                                                         <  2:39420/101‑1          CCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCATAAGCGCTA                                                                                        >  1:679110/1‑101          CCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCATAAGCGCTA                                                                                        <  2:439313/101‑1          CCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCATAAGCGCTA                                                                                        <  1:1351710/101‑1           CTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCATAAGCTCTAA                                                                                       >  1:1105613/1‑101           CTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCATAAGCGCTAA                                                                                       >  2:467021/1‑101           CTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCATAAGCGCTAA                                                                                       >  2:431647/1‑101                AGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCATAAGCGCTAACTTAA                                                                                  <  2:72491/101‑1                        ATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTG                                                                          <  1:482428/101‑1                          TTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGT                                                                        <  1:1095448/101‑1                           TACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTA                                                                       <  1:1536789/101‑1                                 GGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGC                                                                 <  2:981661/101‑1                                   CAAGCAGCACACTTTGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCT                                                               <  2:1030344/101‑1                                   CAAGCAGCACACTTTGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCT                                                               <  2:543509/101‑1                                       CAGCACACTTTGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATA                                                           >  1:512659/1‑101                                        AGCACACTTTGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATAT                                                          <  1:388630/101‑1                                           ACACTTTGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGAT                                                       >  2:616974/1‑101                                            CACTTTGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATT                                                      <  1:1571236/101‑1                                             ACTTTGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTT                                                     >  2:1358584/1‑101                                              CTTTGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTA                                                    >  2:616765/1‑101                                                 TGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACG                                                 <  1:246448/101‑1                                                 TGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACG                                                 >  1:1709242/1‑101                                                   CGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTG                                               <  2:595097/101‑1                                                      CTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCG                                            >  1:1606130/1‑101                                                               GGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAC                                   >  1:384841/1‑101                                                               GGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTAC                                   >  2:1153046/1‑101                                                                  TTCCTGACTGAAGCCCGGCAGTGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCT                                >  2:1002595/1‑101                                                                      TGACTGAAGCCCGGCAGTGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACG                            <  1:1132931/101‑1                                                                      TGACTGAAGCCCGGCAGTGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACG                            >  1:66088/1‑101                                                                      TGACTGAAGCCCGGCAGTGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACG                            >  2:1176492/1‑101                                                                        ACTGAAGCCCGGCAGTGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGAT                          <  2:1124133/101‑1                                                                        ACTGAAGCCCGGCAGTGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGAT                          <  1:1268924/101‑1                                                                           GAAGCCCGGCAGTGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAAC                       <  2:1584432/101‑1                                                                            AAGCCCGGCAGTGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACT                      <  1:1283774/101‑1                                                                            AAGCCCGGCAGTGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACT                      >  1:1562146/1‑101                                                                              GCCCGGCAGTGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGG                    >  2:646202/1‑101                                                                              GCCCGGCAGTGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGG                    >  1:1461865/1‑101                                                                               CCCGGCAGTGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGT                   >  2:267720/1‑101                                                                                      GTGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAAT            >  1:1028021/1‑101                                                                                      GTGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAAT            <  2:946974/101‑1                                                                                       TGGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATT           <  1:1120758/101‑1                                                                                        GGGGAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTC          <  1:431652/101‑1                                                                                           GAGATCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGG       >  1:878425/1‑101                                                                                               TCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCA   >  1:37685/1‑101                                                                                               TCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCA   >  2:81443/1‑101                                                                                               TCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCA   >  2:555473/1‑101                                                                                                CTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCAT  <  2:800232/101‑1                                                                                                CTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCAT  <  1:1181596/101‑1                                                                                                                                                                                                      TCGCAATCGCCTGCCAGCCAGTTATTTACCCAGGCAAGCAGCACACTTTGCGCCTCTTCCCAGGTTTCCTGACTGAAGCCCGGCAGTGGGGAGATCTccc‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/2322249‑2322345 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCAT  >  REL606/2772854‑2772952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.