Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	REL606	1,462,266	IS150 (+) +3 bp	intergenic (‑28/‑311)	mokB ← / → trg	regulatory peptide/methyl‑accepting chemotaxis protein III, ribose and galactose sensor receptor

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 666130	NA (NA)	76 (1.180)	62/200	0.1	100%	noncoding (1443/1443 nt)	IS150	repeat region
	?	REL606	1462266 =	0 (0.000)					intergenic (‑28/‑313)	mokB/trg	regulatory peptide/methyl‑accepting chemotaxis protein III, ribose and galactose sensor receptor
*	?	REL606	= 1462268	0 (0.000)	70 (1.090)	57/200	0.1	100%	intergenic (‑30/‑311)	mokB/trg	regulatory peptide/methyl‑accepting chemotaxis protein III, ribose and galactose sensor receptor
	?	REL606	= 2775877	NA (NA)					noncoding (1/1443 nt)	IS150	repeat region

GTTGTGCTTCATAGCCTTTCTCCTTGCCGGATGGCGGGTAAGAGGCTAAGATCTGAATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/1462169‑1462268 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACT  <  REL606/2775877‑2775779                                                                                                                                                                                                         GTTGTGCTTCATAGCCTTTCTCCTTGCCGGATGGCGGGTAAGAGGCTAAGATCTGAATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTT                                                                                                    <  1:207799/101‑1   TGTGCTTCATAGCCTTTCTCCTTGCCGGATGGCGGGTAAGAGGCTAAGATCTGAATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGT                                                                                                  >  2:272736/1‑101     TGCTTCATAGCCTTTCTCCTTGCCGGATGGCGGGTAAGAGGCTAAGATCTGAATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTAC                                                                                                >  2:1031730/1‑101        TTCATAGCCTTTCTCCTTGCCGGATGGCGGGTAAGAGGCTAAGATCTGAATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGC                                                                                             >  1:1634913/1‑101         TCATAGCCTTTCTCCTTGCCGGATGGCGGGTAAGAGGCTAAGATCTGAATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCA                                                                                            >  2:96157/1‑101          CATAGCCTTTCTCCTTGCCGGATGGCGGGTAAGAGGCTAAGATCTGAATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCAC                                                                                           >  2:471471/1‑101           ATAGCCTTTCTCCTTGCCGGATGGCGGGTAAGAGGCTAAGATCTGAATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACC                                                                                          >  1:136374/1‑101                CTTTCTCCTTGCCGGATGGCGGGTAAGAGGCTAAGATCTGAATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTT                                                                                     >  1:592095/1‑101                CTTTCTCCTTGCCGGATGGCGGGTAAGAGGCTAAGATCTGAATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTT                                                                                     >  1:1053603/1‑101                CTTTCTCCTTGCCGGATGCCGGGTAAGAGGCTAAGATCTGAATTGCTAGGTTCATTCATTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTT                                                                                     <  2:918856/101‑1                 TTTCTCCTTGCCGGATGGCGGGTAAGAGGCTAAGATCTGAATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTT                                                                                    <  2:1672447/101‑1                 TTTCTCCTTGCCGGATGGCGGGTAAGAGGCTAAGATCTGAATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTT                                                                                    >  2:528988/1‑101                 TTTCTCCTTGCCGGATGGCGGGTAAGAGGCTAAGATCTGAATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTT                                                                                    <  1:572994/101‑1                      CCTTGCCGGATGGCGGGTAAGAGGCTAAGATCTGAATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCCCCCATTTTGTTGG                                                                               <  2:4289/101‑1                      CCTTGCCGGATGGCGGGTAAGAGGCTAAGATCTGAATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGG                                                                               <  1:12332/101‑1                       CTTGCCGGATGGCGGGTAAGAGGCTAAGATCTGAATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGA                                                                              >  1:992315/1‑101                        TTGCCGGATGGCGGGTAAGAGGCTAAGATCTGAATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGAC                                                                             >  1:1136753/1‑101                         TGCCGGATGGCGGGTAAGAGGCTAAGATCTGAATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGACG                                                                            <  2:1676314/101‑1                         TGCCGGATGGCGGGTAAGAGGCTAAGATCTGAATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGACG                                                                            <  1:96157/101‑1                         TGCCGGATGGCGGGTAAGAGGCTAAGATCTGAATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGACG                                                                            <  2:1192439/101‑1                           CCGGATGGCGGGTAAGAGGCTAAGATCTGAATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGACGAT                                                                          >  1:1011269/1‑101                               ATGGCGGGTAAGAGGCTAAGATCTGAATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGACGATGAAA                                                                      <  1:528695/101‑1                                TGGCGGGTAAGAGGCTAAGATCTGAATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGACGATGAAAT                                                                     <  2:1223176/101‑1                                TGGCGGGTAAGAGGCTAAGATCTGAATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGACGATGAAAT                                                                     <  2:1126809/101‑1                                TGGCGGGTAAGAGGCTAAGATCTGAATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGACGATGAAAT                                                                     >  1:948233/1‑101                                    GGGTAAGAGGCTAAGATCTGAATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAA                                                                 <  2:1189436/101‑1                                     GGTAAGAGGCTAAGATCTGAATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAAT                                                                <  1:401797/101‑1                                     GGTAAGAGGCTAAGATCTGAATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAAT                                                                <  1:1453484/101‑1                                       TAAGAGGCTAAGATCTGAATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAG                                                              >  1:1462872/1‑101                                       TAAGAGGCTAAGATCTGAATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAG                                                              <  1:621932/101‑1                                           AGGCTAAGATCTGAATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCC                                                          <  1:426785/101‑1                                           AGGCTAAGATCTGAATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCC                                                          >  1:1071604/1‑101                                           AGGCTAAGATCTGAATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCC                                                          >  2:334009/1‑101                                            GGCTAAGATCTGAATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCT                                                         <  1:952411/101‑1                                            GGCTAAGATCTGAATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCT                                                         <  2:46648/101‑1                                                AAGATCTGAATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATA                                                     >  1:1405576/1‑101                                                 AGATCTGAATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATAT                                                    <  2:592090/101‑1                                                 AGATCTGAATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATAT                                                    >  2:860075/1‑101                                                 AGATCTGAATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATAT                                                    <  1:125326/101‑1                                                    TCTGAATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTC                                                 <  1:1620359/101‑1                                                      TGAATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAA                                               >  2:1702490/1‑101                                                        AATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAG                                             <  1:189433/101‑1                                                        AATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAG                                             <  1:408274/101‑1                                                        AATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAG                                             >  2:396807/1‑101                                                        AATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAG                                             <  2:923718/101‑1                                                          TTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCC                                           >  1:1150802/1‑101                                                          TTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCC                                           <  2:243151/101‑1                                                            GCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAA                                         <  1:1707773/101‑1                                                              TAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAA                                       <  2:992310/101‑1                                                              TAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAA                                       >  1:1044863/1‑101                                                                 GTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATAC                                    <  1:528993/101‑1                                                                  TTCATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACC                                   <  1:296676/101‑1                                                                     ATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTT                                <  1:860080/101‑1                                                                     ATTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTT                                >  1:1710974/1‑101                                                                      TTCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTT                               <  1:1266556/101‑1                                                                       TCGTTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTT                              <  2:1634907/101‑1                                                                          TTGGCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAA                           >  2:298966/1‑101                                                                             GCCTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAG                        <  2:1462866/101‑1                                                                               CTCGGTTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCG                      >  2:1576618/1‑101                                                                                  GGTTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCT                   <  1:396812/101‑1                                                                                  GGTTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCT                   >  2:666770/1‑101                                                                                    TTGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTG                 <  2:542623/101‑1                                                                                     TGATAGAAATATCGGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGA                <  2:573307/101‑1                                                                                         AGAAATATCGGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTC            >  1:222657/1‑101                                                                                         AGAAATATCGGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTC            <  1:785922/101‑1                                                                                          GAAATATCGGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCG           >  1:403131/1‑101                                                                                           AAATATCGGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGT          >  2:1275274/1‑101                                                                                             ATATCGGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGA        <  1:132194/101‑1                                                                                                  GGTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCAC   >  1:1684847/1‑101                                                                                                   GTTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACT  <  1:64776/101‑1                                                                                                                                                                                                         GTTGTGCTTCATAGCCTTTCTCCTTGCCGGATGGCGGGTAAGAGGCTAAGATCTGAATTGCTAGGTTCATTCGTTGGCCTCGGTTGATAGAAATATCGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/1462169‑1462268 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACT  <  REL606/2775877‑2775779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.