Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	REL606	896,463	IS150 (+) +3 bp	coding (159‑161/432 nt)	ECB_00838 →	putative phage tail protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 666130	NA (NA)	79 (1.230)	63/200	0.1	100%	noncoding (1443/1443 nt)	IS150	repeat region
	?	REL606	896463 =	0 (0.000)					coding (159/432 nt)	ECB_00838	putative phage tail protein
*	?	REL606	= 896465	0 (0.000)	62 (0.980)	53/196	0.2	100%	coding (161/432 nt)	ECB_00838	putative phage tail protein
	?	REL606	= 2775877	NA (NA)					noncoding (1/1443 nt)	IS150	repeat region

CCGGACAAACTGCATCTGTTTGTGGATAACGGTTCGCTGGTTGCCACGGGGGCCAGCTCCATGTCGTGGGAGTACCGTTACACCCTGAACGCGGTGATtg‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/896368‑896465 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCAC  <  REL606/2775877‑2775780                                                                                                                                                                                                      CCGGACAAACTGCATCTGTTTGTGGATAACGGTTCGCTGGTTGCCACGGGGGCCAGCTCCATGTCGTGGGAGTACCGTTACACCCTGAACGCGGTGATTGT                                                                                                 >  1:1432785/1‑101  CGGACAAACTGCATCTGTTTGTGGATAACGGTTCGCTGGTTGCCACGGGGGCCAGCTCCATGTCGTGGGAGTACCATTACACCCTGAACGCGGTGATTGTA                                                                                                <  1:1533359/101‑1     ACAAACTGCATCTGTTTGTGGATAACGGTTCGCTGGTTGCCACGGGGGCCAGCTCCATGTCGTGGGAGTACCGTTACACCCTGAACGCGGTGATTGTACTG                                                                                             >  2:738597/1‑101       AAACTGCATCTGTTTGTGGATAACGGTTCGCTGGTTGCCACGGGGGCCAGCTCCATGTCGTGGGAGTACCGTTACACCCTGAACGCGGTGATTGTACTGCA                                                                                           >  2:402972/1‑101         ACTGCATCTGTTTGTGGATAACGGTTCGCTGGTTGCCACGGGGGCCAGCTCCATGTCGTGGGAGTACCGTTACACCCTGAACGCGGTGATTGTACTGCACC                                                                                         >  1:1629947/1‑101          CTGCATCTGTTTGTGGATAACGGTTCGCTGGTTGCCACGGGGGCCAGCTCCATGTCGTGGGAGTACCGTTACACCCTGAACGCGGTGATTGTACTGCACCC                                                                                        >  2:1145157/1‑101          CTGCATCTGTTTGTGGATAACGGTTCGCTGGTTGCCACGGGGGCCAGCTCCATGCCCTGGGAGTACCGTTACACCCTGAACGCGGTGATTGTACTGCACCC                                                                                        >  1:841818/1‑101            GCATCTGTTTGTGGATAACGGTTCGCTGGTTGCCACGGGGGCCAGCTCCATGTCGTGGGAGTACCGTTACACCCTGAACGCGGTGATTGTACTGCACCCAT                                                                                      >  1:788789/1‑101             CATCTGTTTGTGGATAACGGTTCGCTGGTTGCCACGGGGGCCAGCTCCATGTCGTGGGAGTACCGTTACACCCTGAACGCGGTGATTGTACTGCACCCATT                                                                                     <  2:902127/101‑1              ATCTGTTTGTGGATAACGGTTCGCTGGTTGCCACGGGGGCCAGCTCCATGTCGTGGGTGTACCGTTACACCCTGAACGCGGTGATTGAACTGCACCCATct                                                                                    >  1:554035/1‑99                CTGTTTGTGGATAACGGTTCGCTGGTTGCCACGGGGGCCAGCTCCATGTCGTGGGAGTACCGTTACACCCTGAACGCGGTGATTGTACTGCACCCATTTTG                                                                                  >  1:566649/1‑101                 TGTTTGTGGATAACGGTTCGCTGGTTGCCACGGGGGCCAGCTCCATGTCGTGGGAGCACCGTTACACCCTGAACGCGGTGATTGTACTGCACCCATTTTGT                                                                                 >  2:1153526/1‑101                   TTTGTGGATAACGGTTCGCTGGTTGCCACGGGGGCCAGCTCCATGTCGTGGGAGTACCGTTACACCCTGAACGCGGTGATTGTACTGCACCCATTTTGTTG                                                                               <  2:35352/101‑1                    TTGTGGATAACGGTTCGCTGGTTGCCACGGGGGCCAGCTCCATGTCGTGGGAGTACCGTTACACCCTGAACGCGGTGATTGTACTGCACCCATTTTGTTGG                                                                              >  2:251653/1‑101                     TGTGGATAACGGTTCGCTGGTTGCCACGGGGGCCAGCTCCATGTCGTGGGAGTACCGTTACACCCTGAACGCGGTGATTGTACTGCACCCATTTTGTTGGA                                                                             >  1:50164/1‑101                     TGTGGATAACGGTTCGCTGGTTGCCACGGGGGCCAGCTCCATGTCGTGGGAGTACCGTTACACCCTGAACGCGGTGATTGTACTGCACCCATTTTGTTGGA                                                                             <  1:851853/101‑1                       TGGATAACGGTTCGCTGGTTGCCACGGGGGCCAGCTCCATGTCGTGGGAGTACCGTTACACCCTGAACGCGGTGATTGTACTGCACCCATTTTGTTGGACG                                                                           <  2:1404506/101‑1                        aGATAACGGTTCGCTGGTTGCCACGGGGGCCAGCTCCATGTCGTGGGAGTACCGTTTCACCCTGAACGCGGCGATTGTACTGCACCCATTTTGTTGGACGA                                                                          >  2:23162/2‑101                           TAACGGTTCGCTGGTTGCCACGGGGGCCAGCTCCATGTCGTGGGAGTACCGTTACACCCTGAAGGCGGTGATTGTACTGCACCCATTTTGTTGGACGATGA                                                                       <  2:693312/101‑1                           TAACGGTTCGCTGGTTGCCACGGGGGCCAGCTCCATGTCGTGGGAGTACCGTTACACCCTGAACGCGGTGATTGTACTGCACCCATTTTGTTGGACGATGA                                                                       >  2:1189372/1‑101                           TAACGGTTCGCTGGTTGCCACGGGGGCCAGCTCCATGTCGTGGGAGTACCGTTACACCCTGAACGCGGTGATTGTACTGCACCCATTTTGTTGGACGATGA                                                                       <  2:613546/101‑1                            AACGGTTCGCTGGTTGCCACGGGGGCCAGCTCCATGTCGTGGGAGTACCGTTACACCCTGAACGCGGTGATTGTACTGCACCCATTTTGTTGGACGATGAA                                                                      <  1:662756/101‑1                              CGGTTCGCTGGTTGCCACGGGGGCCAGCTCCATGTCGTGGGAGTACCGTTACACCCTGAACGCGGTGATTGTACTGCACCCATTTTGTTGGACGATGAAAT                                                                    <  1:118851/101‑1                              CGGTTCGCTGGTTGCCACGGGGGCCAGCTCCATGTCGTGGGAGTACCGTTACACCCTGAACGCGGTGATTGTACTGCACCCATTTTGTTGGACGATGAAAT                                                                    >  2:1252214/1‑101                                   CGCTGGTTGCCACGGGGGCCAGCTCCATGTCGTGGGAGTACCGTTACACCCTGAACGCGGTGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAAT                                                               <  1:485401/101‑1                                     CTGGTTGCCACGGGGGCCAGCTCCATGTCGTGGGAGTACCGTTACACCCTGAACGCGGTGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAG                                                             <  2:937557/101‑1                                      TGGTTGCCACGGGGGCCAGCTCCATGTCGTGGGAGTACCGTTACACCCTGAACGCGGTGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGC                                                            >  2:951854/1‑101                                       GGTTGCCACGGGGGCCAGCTCCATGTCGTGGGAGTACCGTTACACCCTGAACGCGGTGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCC                                                           >  1:430340/1‑101                                        GTTGCCACGGGGGCCAGCTCCATGTCGTGGGAGTACCGTTACACCCTGAACGCGGTGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCC                                                          <  1:1661363/101‑1                                         TTGCCACGGGGGCCAGCTCCATGTCGTGGGAGTACCGTTACACCCTGAACGCGGTGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCC                                                         <  1:1229944/101‑1                                          TGCCACGGGGGCCAGCTCCATGTCGTGGGAGTACCGTTACACCCTGAACGCGGTGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCT                                                        <  2:1111889/101‑1                                           GCCACGGGGGCCAGCTCCATGTCGTGGGAGTACCGTTACACCCTGAACGCGGTGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTA                                                       >  2:1428778/1‑101                                            CCACGGGGGCCAGCTCCATGTCGTGGGAGTACCGTTACACCCTGAACGCGGTGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAA                                                      >  2:1317936/1‑101                                             cagGGGGGCCAGCTCCATGTCGTGGGAGTACCGTTACACCCTGAACGCGGTGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAAT                                                     <  2:787913/98‑1                                             CACGGGGGCCAGCTCCATGTCGTGGGAGTACCGTTACACCCTGAACGCGGTGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAAT                                                     <  2:1109987/101‑1                                             CACGGGGGCCAGCTCCATGTCGTGGGAGTACCGTTACACCCTGAACGCGGTGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAAT                                                     <  1:1153531/101‑1                                              ACGGGGGCCAGCTCCATGTCGTGGGAGTACCGTTACACCCTGAACGCGGTGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATA                                                    <  2:331891/101‑1                                               CGGGGGCCAGCTCCATGTCGTGGGAGTACCTTTACACCCTGAACGCGGTGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATAT                                                   <  1:1112961/101‑1                                               CGGGGGCCAGCTCCATGTCGTGGGAGTACCGTTACACCCTGAACGCGGTGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATAT                                                   <  2:179346/101‑1                                                GGGGGCCAGCTCCATGTCGTGGGAGTACCGTTACACCCTGAACGCGGTGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATG                                                  <  1:107413/101‑1                                                 GGGGACAGCTCCATCTCGTGTGAGTAGCGTTACACCCTGAACGCGGTGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGT                                                 <  1:191778/101‑1                                                     CCAGCTCCATGTCGTGGGAGTACCGTTACACCCTGAACGCGGTGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAA                                             >  1:1547404/1‑101                                                     CCAGCTCCATGTCGTGGGAGTACCGTTACACCCTGAACGCGGTGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAA                                             >  2:1400486/1‑101                                                     CCAGCTCCATGTCGTGGGAGTACCGTTACACCCTGAACGCGGTGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAA                                             >  2:730606/1‑101                                                      CAGCTCCATGTCGTGGGAGTACCGTTACACCCTGAACGCGGTGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAG                                            <  1:1587880/101‑1                                                         CTCCATGTCGTGGGAGTACCGTTACACCCTGAACGCGGTGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCA                                         >  2:1673875/1‑101                                                            CATGTCGTGGGAGTACCGTTACACCCTGAACGCGGTGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAA                                      >  2:831451/1‑101                                                                  GTGGGAGTACCGTTACACCCTGAACGCGGTGATTGTACTGCTCCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCT                                <  2:1427852/101‑1                                                                   TGGGAGTACCGTTACACCCTGAACGCGGTGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTT                               >  1:1303664/1‑101                                                                    GGGAGTACCGTTACACCCTGAACGCGGTGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGACCCTAATATGTCAAAGCCAAAATACGCTTT                              >  2:1562967/1‑101                                                                         TACCGTTACACCCTGAACGCGGTGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAA                         <  1:402977/101‑1                                                                             GTTACACCCTGAACGCGGTGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCG                     >  1:963733/1‑101                                                                             GTTACACCCTGAACGCGGTGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCG                     >  1:1340714/1‑101                                                                                  ACCCTGAACGCGGTGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTG                >  1:334656/1‑101                                                                                     CTGAACGCGGTGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAG             >  1:1191047/1‑101                                                                                         ACGCGGTGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGT         <  1:1506925/101‑1                                                                                          CGCGGTGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTG        >  1:142172/1‑101                                                                                             GGTGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAAT     <  2:1335484/101‑1                                                                                               TGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATaa   >  2:581999/1‑99                                                                                               TGATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCA   <  2:430335/101‑1                                                                                                GATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCAC  <  1:251653/101‑1                                                                                                GATTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCAC  >  1:588431/1‑101                                                                                                                                                                                                      CCGGACAAACTGCATCTGTTTGTGGATAACGGTTCGCTGGTTGCCACGGGGGCCAGCTCCATGTCGTGGGAGTACCGTTACACCCTGAACGCGGTGATtg‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/896368‑896465 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCAC  <  REL606/2775877‑2775780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.