Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	REL606	651,703	+GAA :: IS150 (+) +2 bp	intergenic (‑83/+176)	ybeB ← / ← phpB	hypothetical protein/predicted alpha‑ribazole‑5'‑P phosphatase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	651703 =	0 (0.000)	67 (1.040)	53/200	0.2	100%	intergenic (‑83/+177)	ybeB/phpB	hypothetical protein/predicted alpha‑ribazole‑5'‑P phosphatase
	?	REL606	= 666130	NA (NA)					noncoding (1443/1443 nt)	IS150	repeat region
*	?	REL606	= 651704	0 (0.000)	101 (1.600) +GA	71/196	0.0	100%	intergenic (‑84/+176)	ybeB/phpB	hypothetical protein/predicted alpha‑ribazole‑5'‑P phosphatase
	?	REL606	3651090 =	NA (NA)					intergenic (‑32/‑48)	hokA/insJ‑4	toxic polypeptide, small/IS150 hypothetical protein

TTTCCGCCAGACACGACTTTGTAGAAATTGTTTTACAAAAATGGCGATGCAATCTGCGGCGCGGGGTGGGATGATAGCCCACTTTCGAAAGCCGATTCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/651801‑651703 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTC  <  REL606/666130‑666034                                                                                                                                                                                                      TTTCCGCCAGACACGACTTTGTAGAAATTGTTTTACAAAAATGGCGATGCAATCTGCGGCGCGGGGTGGGATGATAGCCCACTTTCGAAAGCCGATTCGTG                                                                                                 <  2:1502086/101‑1  TTCCGCCAGACACGACTTTGTAGAAATTGTTTTACAAAAATGGCGATGCAATCTGCGGCGCGGGGTGGGATGATAGCCCACTTTCGAAAGCCGATTCGTGT                                                                                                <  1:1335351/101‑1    CCGCCAGACACGACTTTGTAGAAATTGTTTTACAAAAATGGCGATGCAATCTGCGGCGCGGGGTGGGATGATAGCCCACTTTCGAAAGCCGATTCGTGTAC                                                                                              <  2:466047/101‑1     CGCCAGACACGACTTTGTAGAAATTGTTTTACAAAAATGGCGATGCAATCTGCGGCGCGGGGTGGGATGATAGCCCACTTTCGAAAGCCGATTCGTGTACT                                                                                             <  2:784329/101‑1       CCAGACACGACTTTGTAGAAATTGTTTTACAAAAATGGCGATGCAATCTGCGGCGCGGGGTGGGATGATAGCCCACTTTCGAAAGCCGATTCGTGTACTGA                                                                                           >  1:1667070/1‑101          GACCCGACTTTTTAGAAATTGTTTTAAAAAAATGGCGATGCAATCTTCCGCGCGGGGGGGGATTATAGGCCACTTTCGAAAGCCGATTCGTGTACTGACCC                                                                                        <  1:1477917/101‑1           ACACGACTTTGTAGAAATTGTTTTACAAAAATGGCGATGCAATCTGCGGCGCGGGGTGGGATGATAGCCCACTTTCGAAAGCCGATTCGTGTACTGACCCC                                                                                       >  1:541619/1‑101            CACGACTTTGTAGAAATTGTTTTACAAAAATGGCGATGCAATCTGCGGCGCGGGGTGGGATGATAGCCCACTTTCGAAAGCCGATTCGTGTACTGACCCCA                                                                                      >  1:677627/1‑101                 CTTTGTAGAAATTGTTTTACAAAAATGGCGATGCAATCTGCGGCGCGGGGTGGGATGATAGCCCACTTTCGAAAGCCGATTCGTGTACTGACCCCAAAAAG                                                                                 >  2:435309/1‑101                 CTTTGTAGAAATTGTTTTACAAAAATGGCGATGCAATCTGCGGCGCGGGGTGGGATGATAGCCCACTTTCGAAAGCCGATTCGTGTACTGACCCCAAAAAG                                                                                 <  2:1006252/101‑1                  TTTGTAGAAATTGTTTTACAAAAATGGCGATGCAATCTGCGGCGCGGGGTGGGATGATAGCCCACTTTCGAAAGCCGATTCGTGTACTGACCCCAAAAAGT                                                                                <  1:903278/101‑1                  TTTGAAGAAATTGTTTTAAAAAAATGGCGATGCAAGCTGCGGCGCGGGGTGGGATGATAGCCCACTTTCGAAAGCCGATTCGTGTACTGACCCCAAAAAGT                                                                                <  2:553817/101‑1                   TTGTAGAAATTGTTTTACAAAAATGGCGATGCAATCTGCGGCGCGGGGTGGGATGATAGCCCACTTTCGAAAGCCGATTCGTGTACTGACCCCAAAAAGTT                                                                               >  1:1222219/1‑101                   TTGTAGAAATTGTTTTACAAAAATGGCGATGCAATCTGCGGCGCGGGGTGGGATGATAGCCCACTTTCGAAAGCCGATTCGTGTACTGACCCCAAAAAGTT                                                                               >  1:1116660/1‑101                     GTAGAAATTGTTTTACAAAAATGGCGATGCAATCTGCGGCGCGGGGTGGGATGATAGCCCACTTTCGAAAGCCGATTCGTGTACTGACCCCAAAAAGTTGG                                                                             <  1:513969/101‑1                     GTAGAAATTGTTTTACAAAAATGGCGATGCAATCTGCGGCGCGGGGTGGGATGATAGCCCACTTTCGAAAGCCGATTCGTGTACTGACCCCAAAAAGTTGG                                                                             <  2:396487/101‑1                     GTAGAAATTGTTTTACAAAAATGGCGATGCAATCTGCGGCGCGGGGTGGGATGATAGCCCACTTTCGAAAGCCGATTCGTGTACTGACCCCAAAAAGTTGG                                                                             >  2:954490/1‑101                        GAAATTGTTTTACAAAAATGGCGATGCAATCTGCGGCGCGGGGTGGGATGATAGCCCACTTTCGAAAGCCGATTCGTGTACTGACCCCAAAAAGTTGGACA                                                                          >  1:78626/1‑101                        GAAATTGTTTTACAAAAATGGCGATGCAATCTGCGGCGCGGGGTGGGATGATAGCCCACTTTCGAAAGCCGATTCGTGTACTGACCCCAAAAAGTTGGACA                                                                          <  1:669662/101‑1                         AAATTGTTTTACAAAAATGGCGATGCAATCTGCGGCGCGGGGTGGGATGATAGCCCACTTTCGAAAGCCGATTCGTGTACTGACCCCAAAAAGTTGGACAG                                                                         >  1:469890/1‑101                          AATTGTTTTACAAAAATGGCGATGCAATCTGCGGCGCGGGGTGGGATGATAGCCCACTTTCGAAAGCCGATTCGTGTACTGACCCCAAAAAGTTGGACAGT                                                                        >  1:1702373/1‑101                          AATTGTTTTACAAAAATGGCGATGCAATCTGCGGCGCGGGGTGGGATGATAGCCCACTTTCGAAAGCCGATTCGTGTACTGACCCCAAAAAGTTGGACAGT                                                                        <  1:979030/101‑1                           ATTGTTTTACAAAAATGGCGATGCAATCTGCGGCGCGGGGTGGGATGATAGCCCACTTTCGAAAGCCGATTCGTGTACTGACCCCAAAAAGTTGGACAGTT                                                                       <  1:85466/101‑1                              GTTTTACAAAAATGGCGATGCAATCTGCGGCGCGGGGTGGGATGATAGCCCACTTTCGAAAGCCGATTCGTGTACTGACCCCAAAAAGTTGGACAGTTAAA                                                                    >  1:1212363/1‑101                                TTTACAAAAATGGCGATGCAATCTGCGGCGCGGGGTGGGATGATAGCCCACTTTCGAAAGCCGATTCGTGTACTGACCCCAAAAAGTTGGACAGTTAAACA                                                                  >  1:1387794/1‑101                                    CAAAAATGGCGATGCAATCTGCGGCGCGGGGTGGGATGATAGCCCACTTTCGAAAGCCGATTCGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAG                                                              <  2:399844/101‑1                                    CAAAAATGGCGATGCAATCTGCGGCGCGGGGTGGGATGATAGCCCACTTTCGAAAGCCGATTCGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAG                                                              >  1:281404/1‑101                                     AAAAATGGCGATGCAATCTGCGGCGCGGGGTGGGATGATAGCCCACTTTCGAAAGCCGATTCGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGG                                                             <  2:622977/101‑1                                     AAAAATGGCGATGCAATCTGCGGCGCGGGGTGGGATGATAGCCCACTTTCGAAAGCCGATTCGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGG                                                             <  1:708182/101‑1                                       AAATGGCGATGCAATCTGCGGCGCGGGGTGGGATGATAGCCCACTTTCGAAAGCCGATTCGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCA                                                           >  1:109345/1‑101                                       AAATGGCGATGCAATCTGCGGCGCGGGGTGGGATGATAGCCCACTTTCGAAAGCCGATTCGTGTACTGACCCCAAAAAGCTGGACAGTTAAACACGAGGCA                                                           >  2:1358892/1‑101                                        AATGGCGATGCAATCTGCGGCGCGGGGTGGGATGATAGCCCACTTTCGAAAGCCGATTCGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCAT                                                          <  2:526077/101‑1                                        AATGGCGATGCAATCTGCGGCGCGGGGTGGGATGATAGCCCACTTTCGAAAGCCGATTCGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCAT                                                          >  2:15224/1‑101                                          TGGCGATGCAATCTGCGGCGCGGGGTGGGATGATAGCCCACTTTCGAAAGCCGATTCGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATAT                                                        <  2:460297/101‑1                                            GCGATGCAATCTGCGGCGCGGGGTGGGATGATAGCCCACTTTCGAAAGCCGATTCGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAG                                                      <  1:954495/101‑1                                              GATGCAATCTGCGGCGCGGGGTGGGATGATAGCCCACTTTCGAAAGCCGATTCGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGT                                                    >  2:1418954/1‑101                                               ATGCAATCTGCGGCGCGGGGTGGGATGATAGCCCACTTTCGAAATCCGATTCGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTC                                                   >  1:235837/1‑101                                               ATGCAATCTGCGGCGCGGGGTGGGATGATAGCCCACTTTCGAAAGCCGATTCGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTC                                                   >  1:277307/1‑101                                               ATGCAATCTGCGGCGCGGGGTGGGATGATAGCCCACTTTCGAAAGCCGATTCGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTC                                                   <  1:1479632/101‑1                                               ATGCAATCTGCGGCGCGGGGTGGGATGATAGCCCACTTTCGAAAGCCGATTCGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTC                                                   >  2:765582/1‑101                                                      CTGCGGCGCGGGGTGGGATGATAGCCCACTTTCGAAAGCCGATTCGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCC                                            <  2:541614/101‑1                                                          GGCGCGGGGTGGGATGATAGCCCACTTTCGAAAGCCGATTCGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATA                                        <  1:1091843/101‑1                                                                GGGTGGGATGATAGCCCACTTTCGAAAGCCGATTCGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAAT                                  <  2:1406885/101‑1                                                                GGGTGGGATGATAGCCCACTTTCGAAAGCCGATTCGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAAT                                  <  2:469885/101‑1                                                                GGGTGGGATGATAGCCCACTTTCGAAAGCCGATTCGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAAT                                  <  2:1574220/101‑1                                                                 gggGGGATGATAGCCCACTTTCGAAAGCCGATTCGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATT                                 <  2:406845/98‑1                                                                 GGTGGGATGATAGCCCACTTTCGAAAGCCGATTCGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATT                                 <  2:793544/101‑1                                                                        TGATAGCCCACTTTCGAAAGCCGATTCGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCA                          <  1:877436/101‑1                                                                        TGATAGCCCACTTTCGAAAGCCGATTCGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCA                          >  2:948982/1‑101                                                                        TGATAGCCCACTTTCGAAAGCCGATTCGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCA                          >  2:913505/1‑101                                                                            AGCCCACTTTCGAAAGACGATTCGTGTACTGGCCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATGCCGATATTCAATTGGAGTCAGACC                      <  2:1170337/101‑1                                                                              CCCACTTTCGAAAGCCGATTCGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTT                    <  2:1116655/101‑1                                                                                 ACTTTCGAAAGCCGATTCGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTGCGATATTCAATTGGAGTCAGACCTTTTA                 >  1:658082/1‑101                                                                                  CTTTCGAAAGCCGATTCGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAA                >  1:1204198/1‑101                                                                                   TTTCGAAAGCCGATTCGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAAT               >  2:813045/1‑101                                                                                     TCGAAAGCCGATTCGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTT             <  1:1608945/101‑1                                                                                      CGAAAGCCGATTCGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGA‑GCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCA           >  1:1176793/1‑101                                                                                       GAAAGCCGATTCGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCA           <  2:85859/101‑1                                                                                       GAAAGCCGATTCGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCA           <  1:1341746/101‑1                                                                                        AAAGCCGATTCGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAG          <  1:1120890/101‑1                                                                                        AAAGCCGATTCGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAG          >  1:302046/1‑101                                                                                             CGATTCGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAA     >  1:1686589/1‑101                                                                                             CGATTCGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAA     >  1:1347376/1‑101                                                                                              GATTCGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAAT    <  1:787489/101‑1                                                                                              GATTCGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAAT    >  2:1266150/1‑101                                                                                              GATTCGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGACAGACCTTTTAATTTCAGGCTAAT    >  1:1312546/1‑101                                                                                                TTCGTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTC  <  1:15224/101‑1                                                                                                                                                                                                      TTTCCGCCAGACACGACTTTGTAGAAATTGTTTTACAAAAATGGCGATGCAATCTGCGGCGCGGGGTGGGATGATAGCCCACTTTCGAAAGCCGATTCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/651801‑651703 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACCTTTTAATTTCAGGCTAATTC  <  REL606/666130‑666034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.