Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	REL606	1,102,032	IS150 (–) +3 bp	coding (756‑758/1065 nt)	phoH →	conserved protein with nucleoside triphosphate hydrolase domain

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 590471	NA (NA)	65 (1.010)	53/200	0.2	100%	noncoding (1977/1977 nt)	IS150	repeat region
	?	REL606	= 1102034	0 (0.000)					coding (758/1065 nt)	phoH	conserved protein with nucleoside triphosphate hydrolase domain
*	?	REL606	664688 =	NA (NA)	80 (1.240)	59/200	0.1	100%	noncoding (1/1443 nt)	IS150	repeat region
	?	REL606	1102032 =	0 (0.000)					coding (756/1065 nt)	phoH	conserved protein with nucleoside triphosphate hydrolase domain

GAATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/590375‑590471 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTCCCTATTTCCGGTCGCAGGCAGTACTGCATAAAGGAAGCCCCTAAGCGCCGGACCAGCACGTCGTAGACCGGGCGAAAATAGGGAGCAAACTTTTCTG  <  REL606/1102034‑1101935                                                                                                                                                                                                       GAATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACATTCC                                                                                                  >  2:770438/1‑101  AATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACATTCCC                                                                                                 >  1:30035/1‑101     TAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACATTCCCTAT                                                                                              >  2:993896/1‑101     TAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACATTCCCTAT                                                                                              <  2:770126/101‑1      AGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACATTCCCTATT                                                                                             >  2:1267317/1‑101        CCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACATTCCCTATTTC                                                                                           >  2:390230/1‑101            AAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACATTCCCTATTTCCGGT                                                                                       >  2:1633035/1‑101            AAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACATTCCCTATTTCCGGT                                                                                       <  1:1212455/101‑1             AATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACATTCCCTATTTCCGGTC                                                                                      >  2:379135/1‑101               TTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACATTCCCTATTTCCGGTCGC                                                                                    >  1:1495242/1‑101                TAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACATTCCCTATTTCCGGTCGCA                                                                                   >  1:579815/1‑101                 AAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACATTCCCTATTTCCGGTCGCAG                                                                                  <  2:1365015/101‑1                 AAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACATTCCCTATTTCCGGTCGCAG                                                                                  <  1:1209434/101‑1                   AAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACATTCCCTATTTCCGGTCGCAGGC                                                                                <  1:865900/101‑1                    AGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACATTCCCTATTTCCGGTCGCAGGCA                                                                               >  1:853611/1‑101                        CTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACATTCCCTATTTCCGGTCGCAGGCAGTAC                                                                           <  2:475252/101‑1                        CTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACATTCCCTATTTCCGGTCGCAGGCAGTAC                                                                           <  1:1561233/101‑1                        CTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACATTCCCTATTTCCGGTCGCAGGCAGTAC                                                                           <  1:662979/101‑1                        CTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACATTCCCTATTTCCGGTCGCAGGCAGTAC                                                                           <  1:177431/101‑1                         TGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACATTCCCTATTTCCGGTCGCAGGCAGTACT                                                                          >  1:854228/1‑101                           ACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACATTCCCTATTTCCGGTCGCAGGCAGTACTGC                                                                        >  1:1091035/1‑101                            CTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACATTCCCTATTTCCGGTCGCAGGCAGTACTGCA                                                                       >  1:98680/1‑101                               CAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACATTCCCTATTTCCGGTCGCAGGCAGTACTGCATAA                                                                    <  2:592922/101‑1                               CAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACATTCCCTATTTCCGGTCGCAGGCAGTACTGCATAA                                                                    <  2:20918/101‑1                                       TATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACATTCCCTATTTCCGGTCGCAGGCAGTACTGCATAAAGGAAGCC                                                            <  2:937763/101‑1                                            GAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACATTCCCTATTTCCGGTCGCAGGCAGTACTGCATAAAGGAAGCCCCTAA                                                       <  2:579810/101‑1                                              ATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACATTCCCTATTTCCGGTCGCAGGCAGTACTGCATAAAGGAAGCCCCTAAGC                                                     <  2:1438364/101‑1                                                 AGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACATTCCCTATTTCCGGTCGCAGGCAGTACTGCATAAAGGAAGCCCCTAAGCGCC                                                  <  2:854223/101‑1                                                  GACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACATTCCCTATTTCCGGTCGCAGGCAGTACTGCATAAAGGAAGCCCCTAAGCGCCG                                                 <  2:1520008/101‑1                                                  GACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACATTCCCTATTTCCGGTCGCAGGCAGTACTGCATAAAGGAAGCCCCTAAGCGCCG                                                 >  2:1240908/1‑101                                                  GACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACATTCCCTATTTCCGGTCGCAGGCAGTACTGCATAAAGGAAGCCCCTAAGCGCCG                                                 <  2:1335190/101‑1                                                    CCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACATTCCCTATTTCCGGTCGCAGGCAGTACTGCATAAAGGAAGCCCCTAAGCGCCGGA                                               >  2:1100399/1‑101                                                       ATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACATTCCCTATTTCCGGTCGCAGGCAGTACTGCATAAAGGAAGCCCCTAAGCGCCGGACCA                                            >  1:43735/1‑101                                                         ATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACATTCCCTATTTCCGGTCGCAGGCAGTACTGCATAAAGGAAGCCCCTAAGCGCCGGACCAGC                                          <  1:390235/101‑1                                                          TGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACATTCCCTATTTCCGGTCGCAGGCAGTACTGCATAAAGGAAGCCCCTAAGCGCCGGACCAGCA                                         >  2:431956/1‑101                                                           GCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACATTCCCTATTTCCGGTCGCAGGCAGTACTGCATAAAGGAAGCCCCTAAGCGCCGGACCAGCAC                                        <  1:63225/101‑1                                                            CCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACATTCCCTATTTCCGGTCGCAGGCAGTACTGCATAAAGGAAGCCCCTAAGCGCCGGACCAGCACG                                       >  2:1102842/1‑101                                                              TCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACATTCCCTATTTCCGGTCGCAGGCAGTACTGCATAAAGGAAGCCCCTAAGCGCCGGACCAGCACGTC                                     >  2:1038061/1‑101                                                                 TGTTTAACTGTCCAACTTTTTGGGGTCAGTACATTCCCTATTTCCGGTCGCAGGCAGTACTGCATAAAGGAAGCCCCTAAGCGCCGGACCAGCACGTCGTA                                  >  1:1485322/1‑101                                                                 TGTTTAACTGTCCAACTTTTTGGGGTCAGTACATTCCCTATTTCCGGTCGCAGGCAGTACTGCATAAAGGAAGCCCCTAAGCGCCGGACCAGCACGTCGTA                                  <  2:731532/101‑1                                                                 TGTTTAACTGTCCAACTTTTTGGGGTCAGTACATTCCCTATTTCCGGTCGCAGGCAGTACTGCATAAAGGAAGCCCCTAAGCGCCGGACCAGCACGTCGTA                                  >  1:802699/1‑101                                                                  GTTTAACTGTCCAACTTTTTGGGGTCAGTACATTCCCTATTTCCGGTCGCAGGCAGTACTGCATAAAGGAAGCCCCTAAGCGCCGGACCAGCACGTCGTAG                                 >  1:1558813/1‑101                                                                     TAACTGTCCAACTTTTTGGGGTCAGTACATTCCCTATTTCCGGTCGCAGGCAGTACTGCATAAAGGAAGCCCCTAAGCGCCGGACCAGCACGTCGTAGACC                              <  1:666886/101‑1                                                                       ACTGTCCAACTTTTTGGGGTCAGTACATTCCCTATTTCCGGTCGCAGGCAGTACTGCATAAAGGAAGCCCCTAAGCGCCGGACCAGCACGTCGTAGACCGG                            <  2:854211/101‑1                                                                        CTGTCCAACTTTTTGGGGTCAGTACATTCCCTATTTCCGGTCGCAGGCAGTACTGCATAAAGGAAGCCCCTAAGCGCCGGACCAGCACGTCGTAGACCGGG                           <  2:1091030/101‑1                                                                          GTCCAACTTTTTGGGGTCAGTACATTCCCTATTTCCGGTCGCAGGCAGTACTGCATAAAGGAAGCCCCTAAGCGCCGGACCAGCACGTCGTAGACCGGGCG                         >  1:1087794/1‑101                                                                          GTCCAACTTTTTGGGGTCAGTACATTCCCTATTTCCGGTCGCAGGCAGTACTGCATAAAGGAAGCCCCTAAGCGCCGGACCAGCACGTCGTAGACCGGGCG                         >  1:1324031/1‑101                                                                           TCCAACTTTTTGGGGTCAGTACATTCCCTATTTCCGGTCGCAGGCAGTACTGCATAAAGGAAGCCCCTAAGCGCCGGACCAGCACGTCGTAGACCAGGCGA                        >  2:1400278/1‑101                                                                              AACTTTTTGGGGTCAGTACATTCCCTATTTCCGGTCGCAGGCAGTACTGCATAAAGGAAGCCCCTAAGCGCCGGACCAGCACGTCGTAGACCGGGCGAAAA                     >  1:279339/1‑101                                                                               ACTTTTTGGGGTCAGTACATTCCCTATTTCCGGTCGCAGGCAGTACTGCATAAAGGAAGCCCCTAAGCGCCGGACCAGCACGTCGTAGACCGGGCGAAAAT                    <  1:1557143/101‑1                                                                                CTTTTTGGGGTCAGTACATTCCCTATTTCCGGTCGCAGGCAGTACTGCATAAAGGAAGCGCCTAAGCGCCGGACCAGCACGTCGTAGACCGAGCGAAGATA                   >  1:140282/1‑101                                                                                 TTTTTGGGGTCAGTACATTCCCTATTTCCGGTCGCAGGCAGTACTGCATAAAGGAAGCCCCTAAGCGCCGGACCAGCACGTCGTAGACCGGGCGAAAATAG                  <  2:1361498/101‑1                                                                                 TTTTTGGGGTCAGTACATTCCCTATTTCCGGTCGCAGGCAGTACTGCATAAAGGAAGCCCCTAAGCGCCGGACCAGCACGTCGTAGACCGGGCGAAAATAG                  <  1:631469/101‑1                                                                                  TTTTGGGGTCAGTACATTCCCTATTTCCGGTCGCAGGCAGTACTGCATAAAGGAAGCCCCTAAGCGCCGGACCAGCACGTCGTAGACCGGGCGAAAATAGG                 <  2:1480805/101‑1                                                                                  TTTTGGGGTCAGTACATTCCCTATTTCCGGTCGCAGGCAGTACTGCATAAAGGAAGCCCCTAAGCGCCGGACCAGCACGTCGTAGACCGGGCGAAAATAGG                 <  1:1633041/101‑1                                                                                   TTTGGGGTCAGTACATTCCCTATTTCCGGTCGCAGGCAGTACTGCATAAAGGAAGCCCCTAAGCGCCGGACCAGCACGTCGTAGACCGGGCGAAAATAGGG                <  1:1625979/101‑1                                                                                     TGGGGTCAGTACATTCCCTATTTCCGGTCGCAGGCAGTACTGCATAAAGGAAGCCCCTAAGCGCCGGACCAGCACGTCGTAGACCGGGCGAAAATAGGGAG              >  1:1138550/1‑101                                                                                     TGGGGTCAGTACATTCCCTATTTCCGGTCGCAGGCAGTACTGCATAAAGGAAGCCCCTAAGCGCCGGACCAGCACGTCGTAGACCGGGCGAAAATAGGGAG              >  2:811398/1‑101                                                                                       GGGTCAGTACATTCCCTATTTCCGGTCGCAGGCAGTACTGCATAAAGGAAGCCCCTAAGCGCCGGACCAGCACGTCGTAGACCGGGCGAAAATAGGGAGCA            <  1:1460547/101‑1                                                                                        GGTCAGTACATTCCCTATTTCCGGTCGCAGGCAGTACTGCATAAAGGAAGCCCCTAAGCGCCGGACCAGCACGTCGTAGACCGGGCGAAAATAGGGAGCAA           <  2:30035/101‑1                                                                                           CAGTACATTCCCTATTTCCAGTCGCAGGCAGTACTGCATAAAGGAAGCCCCTAAGCGCCGGACCAGCACGTCGTAGACCGGGCGAAAATAGGGAGCAAACT        <  1:106121/101‑1                                                                                            AGTACATTCCCTATTTCCGGTCGCAGGCAGTACTGCATAAAGGAAGCCCCTAAGCGCCGGACCAGCACGTCGTAGACCGGGCGAAAATAGGGAGCAAACTT       >  2:1225717/1‑101                                                                                            AGTACATTCCCTATTTCCGGTCGCAGGCAGTACTGCATAAAGGAAGCCCCTAAGCGCCGGACCAGCACGTCGTAGACCGGGCGAAAATAGGGAGCAAACTT       <  2:608573/101‑1                                                                                            AGTACATTCCCTATTTCCGGTCGCAGGCAGTACTGCATAAAGGAAGCCCCTAAGCGCCGGACCAGCACGTCGTAGACCGGGCGAAAATAGGGAGCAAACTT       <  1:425383/101‑1                                                                                                 ATTCCCTATTTCCGGTCGCAGGCAGTACTGCATAAAGGAAGCCCCTAAGCGCCGGACCAGCACGTCGTAGACCGGGCGAAAATAGGGAGCAAACTTTTCTG  >  2:1024570/1‑101                                                                                                                                                                                                       GAATTAGCCTGAAATTAAAAGGTCTGACTCCAATTGAATATCGGAATCAGACCTATATGCCTCGTGTTTAACTGTCCAACTTTTTGGGGTCAGTACA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/590375‑590471 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTCCCTATTTCCGGTCGCAGGCAGTACTGCATAAAGGAAGCCCCTAAGCGCCGGACCAGCACGTCGTAGACCGGGCGAAAATAGGGAGCAAACTTTTCTG  <  REL606/1102034‑1101935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.