Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	REL606	502,543	IS150 (–) +3 bp	coding (848‑850/1095 nt)	ybbB ←	tRNA 2‑selenouridine synthase, selenophosphate‑dependent

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	502543 =	2 (0.030)	69 (1.070)	50/200	0.3	97.1%	coding (850/1095 nt)	ybbB	tRNA 2‑selenouridine synthase, selenophosphate‑dependent
	?	REL606	588495 =	NA (NA)					noncoding (1/1977 nt)	IS150	repeat region
*	?	REL606	= 502545	2 (0.030)	73 (1.130)	60/200	0.1	97.2%	coding (848/1095 nt)	ybbB	tRNA 2‑selenouridine synthase, selenophosphate‑dependent
	?	REL606	= 590471	NA (NA)					noncoding (1977/1977 nt)	IS150	repeat region

GAGCGCCTGAACGAAGAGTATTTCTTGCGTATGCATCATGATTTTACCCACGCGTACGGCGACGAACAGGGCTGGCAGGAGTATTGCGAATACCTGCATC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/502642‑502543 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCA  >  REL606/588495‑588591                                                                                                                                                                                                       GAGCGCCTGAACGAAGAGTATTTCTTGCGTATGCATCATGATTTTACCCACGCGTACGGCGACGAACAGGGCTGGCAGGAGTATTGCGAATACCTGCATCT                                                                                                  <  1:917595/101‑1  AGCGCCTGAACGAAGAGTATTTCTTGCGTATGCATCATGATTTTACCCACGCGTACGGCGACGAACAGGGCTGGCAGGAGTATTGCGAATACCTGCATCTG                                                                                                 <  2:225732/101‑1       CTGAACGAAGAGTATTTCTTGCGTATGCATCATGATTTTACCCACGCGTACGGCGACGAACAGGGCTGGCAGGAGTATTGCGAATACCTGCATCTGTACTG                                                                                            >  2:557174/1‑101       CTGAACGAAGAGTATTTCTTGCGTATGCATCATGATTTTACCCACGCGTACGGCGACGAACAGGGCTGGCAGGAGTATTGCGAATACCTGCATCTGTACTG                                                                                            >  2:815578/1‑101       CTGAACGAAGAGTATTTCTTGCGTATGCATCATGATTTTACCCACGCGTACGGCGACGAACAGGGCTGGCAGGAGTATTGCGAATACCTGCATCTGTACTG                                                                                            <  2:1205255/101‑1             GAAGAGTATTTCTTGCGTATGCATCATGATTTTACCCACGCGTACGGCGACGAACAGGGCTGGCAGGAGTATTGCGAATACCTGCATCTGTACTGCACCCA                                                                                      >  1:1056988/1‑101              AAGAGTATTTCTTGCGTATGCATCATGATTTTACCCACGCGTACGGCGACGAACAGGGCTGGCAGGAGTATTGCGAATACCTGCATCTGTACTGCACCCAT                                                                                     >  2:969101/1‑101                GAGTATTTCTTGCGTATGCATCATGATTTTACCCACGCGTACGGCGACGAACAGGGCTGGCAGGAGTATTGCGAATACCTGCATCTGTACTGCACCCATTT                                                                                   >  1:1017410/1‑101                GAGTATTTCTCGCGTATGCATCATGATTTTACCCACGCGTACGGCGACGAACAGGGCTGGCAGGAGTATTGCGAATACCTGCATCTGTACTGCACCCATTT                                                                                   >  1:1656537/1‑101                  GTATTTCTTGCGTATGCATCATGATTTTACCCACGCGTACGGCGACGAACAGGGCTGGCAGGAGTATTGCGAATACCTGCATCTGTACTGCACCCATTTTG                                                                                 >  2:590700/1‑101                  GTATTTCTTGCGTATGCATCATGATTTTACCCACGCGTACGGCGACGAACAGGGCTGGCAGGAGTATTGCGAATACCTGCATCTGTACTGCACCCATTTTG                                                                                 >  2:125872/1‑101                   TATTTCTTGCGTATGCATCATGATTTTACCCACGCGTACGGCGACGAACAGGGCTGGCAGGAGTATTGCGAATACCTGCATCTGTACTGCACCCATTTTGT                                                                                >  2:1491281/1‑101                    ATTTCTTGCGTATGCATCATGATTTTACCCACGCGTACGGCGACGAACAGGGCTGGCAGGAGTATTGCGAATACCTGCATCTGTACTGCACCCATTTTGTT                                                                               <  1:1032710/101‑1                    ATTTCTTGCGTATGCATCATGATTTTACCCACGCGTACGGCGACGAACAGGGCTGGCAGGAGTATTGCGAATACCTGCATCTGTACTGCACCCATTTTGTT                                                                               >  2:956669/1‑101                    ATTTCTTGCGTATGCATCATGATTTTACCCACGCGTACGGCGACGAACAGGGCTGGCAGGAGTATTGCGAATACCTGCATCTGTACTGCACCCATTTTGTT                                                                               >  2:100495/1‑101                     TTTCTTGCGTATGCATCATGATTTTACCCACGCGTACGGCGACGAACAGGGCTGGCAGGAGTATTGCGAATCCCTCCATCTGGACTGCACCCATTTTGTTG                                                                              >  2:1165507/1‑101                     TTTCTTGCGTATGCATCATGATTTTACCCACGCGTACGGCGACGAACAGGGCTGGCAGGAGTATTGCGAATACCTGCATCTGTACTGCACCCATTTTGTTG                                                                              >  2:1010210/1‑101                             GTATGCATCATGATTTTACCCACGCGTACGGCGACGAACAGGGCTGGCAGGAGTATTGCGAATACCTGCATCTGTACTGCACCCATTTTGTTGGACGATGA                                                                      >  1:944220/1‑101                             GTATGCATCATGATTTTACCCACGCGTACGGCGACGAACAGGGCTGGCAGGAGTATTGCGAATACCTGCATCTGTACTGCACCCATTTTGTTGGACGATGA                                                                      >  1:942011/1‑101                               ATGCATCATGATTTTACCCACGCGTACGGCGACGAACAGGGCTGGCAGGAGTATTGCGAATACCTGCATCTGTACTGCACCCATTTTGTTGGACGATGAAA                                                                    <  1:596347/101‑1                               ATGCATCATGATTTTACCCACGCGTACGGCGACGAACAGGGCTGGCAGGAGTATTGCGAATACCTGCATCTGTACTGCACCCATTTTGTTGGACGATGAAA                                                                    >  2:1179624/1‑101                               ATGCATCATGATTTTACCCACGCGTACGGCGACGAACAGGGCTGGCAGGAGTATTGCGAATACCTGCATCTGTACTGCACCCATTTTGTTGGACGATGAAA                                                                    >  2:1698955/1‑101                                TGCATCATGATTTTACCCACGCGTACGGCGACGAACAGGGCTGGCAGGAGTATTGCGAATACCTGCATCTGTACTGCACCCATTTTGTTGGACGATGAAAT                                                                   <  2:1558572/101‑1                                    TCATGATTTTACCCACGCGTACGGCGACGAACAGGGCTGGCAGGAGCATTGCGAATACCTGCATCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAA                                                               >  2:1488945/1‑101                                      ATGATTTTACCCACGCGTACGGCGACGAACAGGGCTGGCAGGAGTATTGCGAATACCTGCATCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATA                                                             >  1:1599298/1‑101                                      ATGATTTTACCCACGCGTACGGCGACGAACAGGGCTGGCAGGAGTATTGCGAATACCTGCATCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATA                                                             >  1:1593301/1‑101                                      ATGATTTTACCCACGCGTACGGCGACGAACAGGGCTGGCAGGAGTATTGCGAATACCTGCATCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATA                                                             >  1:417364/1‑101                                        GATTTTACCCACGCGTACGGCGACGAACAGGGCTGGCAGGAGTATTGCGAATACCTGCATCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGC                                                           >  2:280964/1‑101                                             TACCCACGCGTACGGCGACGAACAGGGCTGGCAGGAGTATTGCGAATACCTGCATCTGTACTGCACCCATTTTGTTGGTCGATGAAATGGAATAGCCCCTA                                                      >  1:186958/1‑101                                               CCCACGCGTACGGCGACGAACAGGGCTGGCAGGAGTATTGCGAATACCTGCATCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAAT                                                    <  2:1267746/101‑1                                               CCCACGCGTACGGCGACGAACAGGGCTGGCAGGAGTATTGCGAATACCTGCATCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAAT                                                    >  2:296778/1‑101                                                 CACGCGTACGGCGACGAACAGGGCTGGCAGGAGTATTGCGAATACCTGCATCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATAT                                                  <  1:590705/101‑1                                                 CACGCGTACGGCGACGAACAGGGCTGGCAGGAGTATTGCGAATACCTGCATCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATAT                                                  <  1:956674/101‑1                                                 CACGCGTACGGCGACGAACAGGGCTGGCAGGAGTATTGCGAATACCTGCATCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATAT                                                  <  1:954415/101‑1                                                 CACGCGTACGGCGACGAACAGGGCTGGCAGGAGTATTGCGAATACCTGCATCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATAT                                                  <  1:557179/101‑1                                                 CACGCGTACAGCGACGAACAGGGCTGGCAGGAGTATTGCGAATACCTGCATCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATAT                                                  <  2:603968/101‑1                                                     CGTACGGCGACGAACAGGGCTGGCAGGAGTATTGCGAATACCTGCATCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCA                                              >  2:1066322/1‑101                                                       TACGGCGACGAACAGGGCTGGCAGGAGTATTGCGAATACCTGCATCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAA                                            >  1:1500323/1‑101                                                       TACGGCGACGAACAGGGCTGGCAGGAGTATTGCGAATACCTGCATCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAA                                            >  2:108870/1‑101                                                       TACGGCGACGAACAGGGCTGGCAGGAGTATTGCGAATACCTGCATCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAA                                            <  1:220169/101‑1                                                        ACGGCGACGAACAGGGCTGGCAGGAGTATTGCGAATACCTGCATCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAG                                           <  1:1491287/101‑1                                                        ACGGCGACGAACAGGGCTGGCAGGAGTATTGCGAATACCTGCATCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAG                                           <  2:1268108/101‑1                                                           GCGACGAACAGGGCTGGCAGGAGTATTGCGAATACCTGCATCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCA                                        <  1:118420/101‑1                                                             GACGAACAGGGCTGGCAGGAGTATTGCGAATACCTGCATCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAA                                      >  1:432843/1‑101                                                             GACGAACAGGGCTGGCAGGAGTATTGCGAATACCTGCATCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAA                                      <  2:1656531/101‑1                                                                   CAGGGCTGGCAGGAGTATTGCGAATACCTGCATCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCC                                <  1:1717544/101‑1                                                                    AGGGCTGGCAGGAGTATTGCGAATACCTGCATCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCT                               <  1:1695544/101‑1                                                                      GGCTGGCAGGAGTATTGCGAATACCTGCATCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTT                             <  2:1593295/101‑1                                                                       GCTGGCAGGAGTATTGCGAATACCTGCATCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTT                            <  1:930518/101‑1                                                                        CTGGCAGGAGTATTGCGAATACCTGCATCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTG                           <  1:349524/101‑1                                                                        CTGGCAGGAGTATTGCGAATACCTGCATCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTG                           <  2:35864/101‑1                                                                            CAGGAGTATTGCGAATACCTGCATCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAA                       >  2:686829/1‑101                                                                             AGGAGTATTGCGAATACCTGCATCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAG                      >  2:1713420/1‑101                                                                             AGGAGTATTGCGAATACCTGCATCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAG                      >  2:893011/1‑101                                                                              GGAGTATTGCGAATACCTGCATCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGC                     <  2:1319224/101‑1                                                                                 GTATTGCGAATACCTGCATCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCC                  >  2:1415304/1‑101                                                                                 GTATTGCGAATACCTGCATCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCC                  <  1:246512/101‑1                                                                                   ATTGCGAATACCTGCATCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTT                >  1:529837/1‑101                                                                                     TGCGAATACCTGCATCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGA              <  2:432838/101‑1                                                                                      GCGAATACCTGCATCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAA             <  1:815583/101‑1                                                                                       CGAATACCTGCATCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAG            <  1:686834/101‑1                                                                                          ATACCTGCATCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCG         >  2:1258150/1‑101                                                                                          ATACCTGCATCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCG         >  2:1525523/1‑101                                                                                            ACCTGCATCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTNGAAAANCGCCTTGAAGTCGTG       >  2:1305648/1‑101                                                                                              CTGCATCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAA     <  1:578894/101‑1                                                                                              CTGCATCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAA     <  1:280964/101‑1                                                                                               TGCATCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAAT    >  2:82466/1‑101                                                                                                 CATCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCA  >  2:832101/1‑101                                                                                                 CATCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCA  <  2:942006/101‑1                                                                                                                                                                                                       GAGCGCCTGAACGAAGAGTATTTCTTGCGTATGCATCATGATTTTACCCACGCGTACGGCGACGAACAGGGCTGGCAGGAGTATTGCGAATACCTGCATC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/502642‑502543 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCA  >  REL606/588495‑588591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.