Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	REL606	183,262	IS150 (+) +3 bp	coding (1184‑1186/1518 nt)	dgt →	deoxyguanosinetriphosphate triphosphohydrolase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	183262 =	1 (0.020)	103 (1.600)	73/200	0.0	99.0%	coding (1184/1518 nt)	dgt	deoxyguanosinetriphosphate triphosphohydrolase
	?	REL606	= 590471	NA (NA)					noncoding (1977/1977 nt)	IS150	repeat region
*	?	REL606	= 183264	1 (0.020)	80 (1.260)	60/198	0.1	98.7%	coding (1186/1518 nt)	dgt	deoxyguanosinetriphosphate triphosphohydrolase
	?	REL606	588495 =	NA (NA)					noncoding (1/1977 nt)	IS150	repeat region

CGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGt‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/183166‑183264 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACT  >  REL606/588495‑588593                                                                                                                                                                                                        CGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTG                                                                                                   >  2:132949/1‑101  GTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGT                                                                                                  >  2:1206073/1‑101   TTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTA                                                                                                 <  2:514262/101‑1    TTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTAC                                                                                                <  2:1619519/101‑1    TTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTAC                                                                                                <  2:1413890/101‑1    TTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTAC                                                                                                <  1:1429787/101‑1    TTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTAC                                                                                                >  2:1606457/1‑101     TAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTATACATCCAGATGTGTACT                                                                                               >  2:429031/1‑101     TAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACT                                                                                               <  2:970142/101‑1     TAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACT                                                                                               >  2:933514/1‑101         CATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCAC                                                                                           >  2:484948/1‑101         CATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCAC                                                                                           >  2:1677640/1‑101          ATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACC                                                                                          >  1:1217974/1‑101             CATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCAT                                                                                       >  2:1060175/1‑101              ATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATT                                                                                      >  1:494615/1‑101                 ATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTG                                                                                   >  1:90418/1‑101                   TGGAAGATGCCATCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTT                                                                                 >  1:258636/1‑101                   TGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTT                                                                                 >  1:669237/1‑101                   TGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTT                                                                                 >  1:998457/1‑101                       AGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGAC                                                                             >  2:606527/1‑101                        GATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACG                                                                            >  1:859991/1‑101                         ATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGA                                                                           >  2:1506357/1‑101                            CCATCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGA                                                                        <  1:1412751/101‑1                              AGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAA                                                                      >  2:1414386/1‑101                                  AATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGA                                                                  <  1:853031/101‑1                                   ATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAA                                                                 <  1:964994/101‑1                                   ATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAA                                                                 <  2:659507/101‑1                                         CGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCC                                                           <  1:31319/101‑1                                          GATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATATCCCC                                                          <  1:1060180/101‑1                                          GATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCC                                                          >  2:876531/1‑101                                          GATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCC                                                          <  2:1485754/101‑1                                           ATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCT                                                         >  2:41208/1‑101                                           ATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCT                                                         >  1:1702716/1‑101                                             CTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAA                                                       >  1:749496/1‑101                                              TTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAAT                                                      <  2:338226/101‑1                                              TTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAAT                                                      <  2:1386621/101‑1                                               TCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATA                                                     >  2:365088/1‑101                                               TCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATA                                                     >  2:928173/1‑101                                                CTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATAT                                                    <  1:866064/101‑1                                                CTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATAT                                                    <  2:1154035/101‑1                                                CTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATAT                                                    <  2:173529/101‑1                                                  TAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGT                                                  <  2:1490669/101‑1                                                  TAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGT                                                  >  2:1452637/1‑101                                                    AGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCA                                                <  2:1142371/101‑1                                                    AGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCA                                                >  2:928198/1‑101                                                     GCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAA                                               >  2:369011/1‑101                                                     GCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAA                                               <  1:1606463/101‑1                                                     GCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAA                                               >  1:179490/1‑101                                                      CTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAA                                              <  2:756770/101‑1                                                       TATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTACTATGTCAAAG                                             <  2:1139219/101‑1                                                       TATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAG                                             <  2:426274/101‑1                                                        ATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGC                                            <  1:1017055/101‑1                                                         TATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCC                                           >  2:499645/1‑101                                                            AAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAA                                        <  2:1217969/101‑1                                                            AAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAA                                        >  2:311501/1‑101                                                              AAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAAT                                      <  2:1644986/101‑1                                                              AAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAAT                                      <  2:351641/101‑1                                                               AATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATA                                     <  1:609587/101‑1                                                                   TCGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCT                                 <  1:841191/101‑1                                                                    CGCTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAACAAAAATACCCTT                                >  2:8848/1‑101                                                                      CTGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTT                              >  1:943541/1‑101                                                                       TGTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTG                             <  1:484953/101‑1                                                                        GTAAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGA                            >  1:98490/1‑101                                                                          AAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAA                          <  1:933519/101‑1                                                                          AAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAA                          >  2:429279/1‑101                                                                          AAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAA                          >  2:1574659/1‑101                                                                          AAAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAA                          >  2:42886/1‑101                                                                           AAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAA                         >  1:881851/1‑101                                                                           AAACATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAA                         <  1:998262/101‑1                                                                               ATGTGTTTAGCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGC                     >  1:265347/1‑101                                                                                        GCCATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTC            <  1:1206078/101‑1                                                                                           ATCCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTG         <  2:335479/101‑1                                                                                             CCAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAA       <  1:1459350/101‑1                                                                                              CAGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAAT      <  1:41208/101‑1                                                                                               AGATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATC     >  1:886661/1‑101                                                                                                GATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCA    >  1:1340638/1‑101                                                                                                 ATGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCAC   <  1:1677646/101‑1                                                                                                  TGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACT  >  1:831368/1‑101                                                                                                  TGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACT  >  2:352752/1‑101                                                                                                  TGTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACT  >  2:1641766/1‑101                                                                                                                                                                                                        CGTTTAATCATGCATTATTGGAAGATGCCAGCGAATGCAGCGATCTTCTTAAGCTATATAAAAATGTCGCTGTAAAACATGTGTTTAGCCATCCAGATGt‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/183166‑183264 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAAAAGCGCCTTGAAGTCGTGAATCACT  >  REL606/588495‑588593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.