| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 1,915,108 | (A)8→7 | intergenic (‑218/‑120) | zwf ← / → yebK | glucose‑6‑phosphate 1‑dehydrogenase/predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,915,101 | 0 | A | . | 96.9% | 304.6 / 1.6 | 65 | intergenic (‑211/‑127) | zwf/yebK | glucose‑6‑phosphate 1‑dehydrogenase/predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (2/0); new base . (26/37); total (28/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.82e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCATTTCGATTTCTTTCAGTGC > REL606/1915001‑1915193 | ccACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATGa > 2:1623400/1‑101 (MQ=255) ccGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATGaaaaa > 1:1119716/1‑101 (MQ=255) ccGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATGaaaaa > 1:1513215/1‑101 (MQ=255) cGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATGaaaaaa > 1:145637/1‑101 (MQ=255) cGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATGaaaaaa > 1:264563/1‑101 (MQ=255) cGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATGaaaaaa < 2:199666/101‑1 (MQ=255) tGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATGaaaaaaat < 1:1152459/101‑2 (MQ=255) gCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATGaaaaaaata < 2:1263752/101‑3 (MQ=255) gATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATGaaaaaaataac < 1:964009/101‑5 (MQ=255) gATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATGaaaaaaataac > 1:1640610/1‑97 (MQ=255) gATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATGaaaaaaataac > 2:1090520/1‑97 (MQ=255) aTTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACCCTGATCATGTTATG‑AAAAAAATaaca < 1:149698/101‑1 (MQ=255) aTTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATaaca < 2:125666/101‑1 (MQ=255) aTTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATaaca < 1:931729/101‑1 (MQ=255) ttACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATaacaa < 1:21652/101‑1 (MQ=255) ttACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATaacaa < 1:461103/101‑1 (MQ=255) ttACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATaacaa < 1:998855/101‑1 (MQ=255) tACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATaacaa > 2:213511/1‑101 (MQ=255) tACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATaacaac < 2:1048919/101‑1 (MQ=255) aaaaGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACtttttt < 1:481073/101‑1 (MQ=255) aaGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTAt < 2:1018085/101‑1 (MQ=255) aaGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTAt > 2:1233329/1‑101 (MQ=255) aaGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTAt > 1:574692/1‑101 (MQ=255) cgcgcAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTg > 1:847448/1‑101 (MQ=255) cgcgcAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTg < 1:905210/101‑1 (MQ=255) cgcAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCt < 2:979926/101‑1 (MQ=255) aaaGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCtttt < 1:374467/101‑1 (MQ=255) aaGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTg < 2:104658/101‑1 (MQ=255) aaGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTg < 2:526970/101‑1 (MQ=255) gCGGCAAGACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCAt < 1:428099/101‑1 (MQ=255) gCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTaa > 2:1554522/1‑101 (MQ=255) aaaCTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAacac < 1:1559294/101‑1 (MQ=255) aaaCTGAAAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAacac < 2:1119711/101‑1 (MQ=255) aaCTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACg < 2:729971/101‑1 (MQ=255) aaCTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACg > 1:678525/1‑101 (MQ=255) aCTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACgg < 2:646150/101‑1 (MQ=255) tGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGca < 1:716827/101‑1 (MQ=255) tGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGca > 1:300746/1‑101 (MQ=255) tGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTAATTAACACGGca > 1:287250/1‑101 (MQ=255) aTAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGcaca > 1:1598370/1‑101 (MQ=255) aaaaaaaTCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGcacaca < 1:282885/101‑1 (MQ=255) aaaaaaTCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAg < 2:963338/101‑1 (MQ=255) aaaaaaTCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAg > 2:392895/1‑101 (MQ=255) aaaTCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCg > 1:476484/1‑101 (MQ=255) aaaTCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCg > 2:502291/1‑101 (MQ=255) atcatcGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTa < 1:256409/101‑1 (MQ=255) tcatcGTTTTCCATTCGGTAAAAATCTGACACT‑ATCATGTTATGAAAAAAAA‑AACAACTTTTTTATTTGCTTTTGTCATTAAAACGGCACACAAGCGGAat > 2:617739/1‑101 (MQ=255) catcGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAat > 2:1106940/1‑101 (MQ=255) cGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATc < 1:403977/101‑1 (MQ=255) gTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCt < 2:1608108/101‑1 (MQ=255) gTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCt > 2:620698/1‑101 (MQ=255) gTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCt > 2:1246640/1‑101 (MQ=255) gTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTACCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCt < 2:774753/101‑1 (MQ=255) ttttCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTg > 2:42224/1‑101 (MQ=255) ttCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATGACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGAc > 1:1496553/1‑101 (MQ=255) tCCATTCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACa > 2:67342/1‑101 (MQ=255) ttCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAAc < 2:1309273/101‑1 (MQ=255) ttCGGTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAAc < 2:422845/101‑1 (MQ=255) gTAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCa > 2:1411096/1‑101 (MQ=255) tAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCAt < 2:574687/101‑1 (MQ=255) tAAAAATCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCAt > 1:929799/1‑101 (MQ=255) aaaaaTCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCAtt > 2:589708/1‑101 (MQ=255) aaaaaTCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCAtt > 1:284006/1‑101 (MQ=255) aaaaTCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCAttt < 2:1513209/101‑1 (MQ=255) aaaaTCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCAttt < 2:145637/101‑1 (MQ=255) aaaaTCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCAttt < 1:629087/101‑1 (MQ=255) aaaTCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCATTTc < 1:92919/101‑1 (MQ=255) aaaTCTGACACTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCATTTc > 1:545331/1‑101 (MQ=255) acTGATCATGTTATG‑AAAAAAATGACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCATTTCGAtttcttt < 2:1496547/101‑1 (MQ=255) acTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCATTTCGAtttcttt < 2:678520/101‑1 (MQ=255) cTGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCATTTCGAtttctttc < 2:383845/101‑1 (MQ=255) tGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCATTTCGATTTCTTTCa > 2:792132/1‑101 (MQ=255) tGATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCATTTCGATTTCTTTCa > 1:919781/1‑101 (MQ=255) gATCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCATTTCGATTTCTTTCAg > 2:578321/1‑101 (MQ=255) aTCATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCATTTCGATTTCTTTCAGt > 1:1512531/1‑101 (MQ=255) cATGTTATG‑AAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCATTTCGATTTCTTTCAGTGc < 1:1113249/101‑1 (MQ=255) | CCACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTCGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCATTTCGATTTCTTTCAGTGC > REL606/1915001‑1915193 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |