| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | REL606 | 393,720 | G→A | 100% | R96H (CGC→CAC) | queA → | S‑adenosylmethionine:tRNA ribosyltransferase‑isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 393,720 | 0 | G | A | 100.0% | 374.4 / NA | 109 | R96H (CGC→CAC) | queA | S‑adenosylmethionine:tRNA ribosyltransferase‑isomerase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (58/51); total (58/51) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
Only 100 of 110 total aligned reads displayed.
TGCTGGTTGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCGCGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATG > REL606/393672‑393767 | tGGTTGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCt < 1:1215563/51‑1 (MQ=255) tGGTTGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCt > 1:2386146/1‑51 (MQ=255) tGGTTGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCt > 1:6966505/1‑51 (MQ=255) tGGTTGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCt < 1:6358943/51‑1 (MQ=255) ggTTGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTc > 1:108122/1‑51 (MQ=255) ggTTGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTc < 1:8174446/51‑1 (MQ=255) ggTTGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTc < 1:1638780/51‑1 (MQ=255) gTTGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCg > 1:5131018/1‑51 (MQ=255) ttGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGa < 1:3872409/51‑1 (MQ=255) ttGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGa > 1:6654100/1‑51 (MQ=255) gAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGaaa < 1:8790651/51‑1 (MQ=255) aaCGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAg > 1:7134625/1‑51 (MQ=255) aaCGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAg > 1:10242216/1‑51 (MQ=255) aCGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAgc < 1:6922880/51‑1 (MQ=255) aCGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAgc > 1:6683472/1‑51 (MQ=255) cGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGaaa > 1:646739/1‑48 (MQ=255) cGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAgcg < 1:4386676/51‑1 (MQ=255) cGGATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAgcg < 1:2109235/51‑1 (MQ=255) ggATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAgcgc > 1:10449959/1‑51 (MQ=255) gATGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGcc > 1:3359490/1‑51 (MQ=255) aTGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCa > 1:1280213/1‑51 (MQ=255) aTGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCa > 1:6790284/1‑51 (MQ=255) aTGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCa < 1:1987669/51‑1 (MQ=255) aTGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCa > 1:8892698/1‑51 (MQ=255) aTGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCa < 1:2268748/51‑1 (MQ=255) tGCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCaa > 1:3174635/1‑51 (MQ=255) gCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCaaa > 1:5162498/1‑51 (MQ=255) gCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCaaa > 1:7140109/1‑51 (MQ=255) gCTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCaaa < 1:10885398/51‑1 (MQ=255) cTCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCaaaa < 1:4346962/51‑1 (MQ=255) tCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAAc > 1:882197/1‑51 (MQ=255) tCGACGACAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAAc < 1:3036359/51‑1 (MQ=255) gacgacAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCt < 1:6256485/51‑1 (MQ=255) acgacAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTg > 1:6812464/1‑51 (MQ=255) acgacAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTg > 1:8045047/1‑51 (MQ=255) acgacAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTg < 1:9118917/51‑1 (MQ=255) acgacAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTg < 1:5456567/51‑1 (MQ=255) acgacAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTg < 1:6469794/51‑1 (MQ=255) cgacAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTgg > 1:7410759/1‑51 (MQ=255) cgacAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTgg < 1:1643220/51‑1 (MQ=255) gacAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGgc > 1:8474848/1‑51 (MQ=255) acAACCGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCaaaa < 1:7024638/44‑1 (MQ=37) acAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGgcg > 1:4512420/1‑51 (MQ=255) acAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGgcg > 1:10697425/1‑51 (MQ=255) acAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGgc > 1:1702427/1‑50 (MQ=255) cAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGgcgc > 1:7389478/1‑51 (MQ=255) cAAACGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGgcgc > 1:8193305/1‑51 (MQ=255) aaaCGCATTCTTGCGCATATTCACGCCTCGaa < 1:872991/32‑1 (MQ=255) aaaCGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCa < 1:9444647/51‑1 (MQ=255) aaaCGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCa < 1:6280756/51‑1 (MQ=255) aaaCGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCa < 1:1359738/51‑1 (MQ=255) aaaCGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCa > 1:3724783/1‑51 (MQ=255) aaaCGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCa < 1:3853227/51‑1 (MQ=255) aaaCGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCa < 1:2078780/51‑1 (MQ=255) aaaCGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCa > 1:3083177/1‑51 (MQ=255) aaCGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAg > 1:10394439/1‑51 (MQ=255) aaCGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAg > 1:3516809/1‑51 (MQ=255) aaCGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAg < 1:520633/51‑1 (MQ=255) aCGCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCt < 1:3208746/43‑1 (MQ=255) gCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGaa < 1:5340677/50‑1 (MQ=255) gCATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAAc < 1:5712046/51‑1 (MQ=255) cATTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAAct < 1:145185/51‑1 (MQ=255) aTTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAActg > 1:4182058/1‑51 (MQ=255) aTTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAActg < 1:6250960/51‑1 (MQ=255) aTTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAActg > 1:7212963/1‑51 (MQ=255) aTTCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAActg > 1:10504314/1‑51 (MQ=255) ttCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAActgc < 1:8381987/51‑1 (MQ=255) ttCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAActgc > 1:7591597/1‑51 (MQ=255) ttCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAActgc > 1:1098486/1‑51 (MQ=255) ttCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAActgc > 1:8551101/1‑51 (MQ=255) ttCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAActgc > 1:9070577/1‑51 (MQ=255) ttCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAActgc > 1:5531121/1‑51 (MQ=255) tCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAActgct < 1:7819422/51‑1 (MQ=255) tCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAActgct < 1:6875907/51‑1 (MQ=255) tCTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAActgct > 1:1891404/1‑51 (MQ=255) cTTGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGTAGAActgctg < 1:9437467/51‑1 (MQ=255) ttGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAActgctgc < 1:10219115/51‑1 (MQ=255) ttGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAActgctgc < 1:2576222/51‑1 (MQ=255) tGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAActgctgct < 1:4080024/51‑1 (MQ=255) tGCGCATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAActgctgct > 1:2400546/1‑51 (MQ=255) gcgcATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAActgctgctg > 1:7299205/1‑51 (MQ=255) gcgcATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAActgctgctg < 1:585298/51‑1 (MQ=255) gcgcATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAActgct < 1:1309641/47‑1 (MQ=255) cgcATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTgg > 1:7943570/1‑51 (MQ=255) gcATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTggg > 1:4525147/1‑51 (MQ=255) gcATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTggg < 1:7515068/51‑1 (MQ=255) cATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGc < 1:10326770/51‑1 (MQ=255) cATATTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGc > 1:8496755/1‑51 (MQ=255) tatTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGa > 1:9183351/1‑51 (MQ=255) tatTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGa > 1:10263587/1‑51 (MQ=255) tatTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGa < 1:6082313/51‑1 (MQ=255) tatTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGa > 1:10492172/1‑51 (MQ=255) atTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGAt < 1:3982518/51‑1 (MQ=255) atTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGAt > 1:6102441/1‑51 (MQ=255) atTCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGAt > 1:7530873/1‑51 (MQ=255) ttCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATg > 1:6293182/1‑51 (MQ=255) ttCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATg < 1:7497857/51‑1 (MQ=255) ttCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATg > 1:2351426/1‑51 (MQ=255) ttCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATg < 1:159678/51‑1 (MQ=255) ttCACGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATg < 1:7488550/51‑1 (MQ=255) | TGCTGGTTGAACGGATGCTCGACGACAAACGCATTCTTGCGCATATTCGCGCCTCGAAAGCGCCAAAACCTGGCGCAGAACTGCTGCTGGGCGATG > REL606/393672‑393767 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |