Predicted mutation
evidence seq id position mutation freq annotation gene description
RA REL606 3,684,366 C→A 100% R216S (CGT→AGT)  ECB_03438 → conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6063,684,3660CA100.0% 396.3 / NA 113R216S (CGT→AGT) ECB_03438conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (63/50);  total (63/50)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 113 total aligned reads displayed.

ACTGAAGAGCCGCGCTACCTGGCGCTGACGAACTATTTTGTCGAACAGCGTGGTGCGCAACCGCACTATTACGACCAAGAATATGAAAAGCGCGGG  >  REL606/3684318‑3684413
                                                |                                               
aCTGAAGAGCCGCGCTACCTGGCGCTGACGAACTATTTTGTCGAACAGAgt                                               >  1:10022838/1‑51 (MQ=255)
aCTGAAGAGCCGCGCTACCTGGCGCTGACGAACTATTTTGTCGAACAGAgt                                               <  1:2575655/51‑1 (MQ=255)
aCTGAAGAGCCGCGCTACCTGGCGCTGACGAACTATTTTGTCGAACAGAgt                                               >  1:4193812/1‑51 (MQ=255)
aCTGAAGAGCCGCGCTACCTGGCGCTGACGAACTATTTTGTCGAACAGAgt                                               <  1:4631988/51‑1 (MQ=255)
 cTGAAGAGCCGCGCTACCTGGCGCTGACGAACTATTTTGTCGAACAGAgtg                                              >  1:7441728/1‑51 (MQ=255)
  tGAAGAGCCGCGCTACCTGGCGCTGACGAACTATTTTGTCGAACAGAgtgg                                             >  1:2509138/1‑51 (MQ=255)
   gAAGAGCCGCGCTACCTGGCGCTGACGAACTATTTTGTCGAACAGAgtggt                                            >  1:5769185/1‑51 (MQ=255)
   gAAGAGCCGCGCTACCTGGCGCTGACGAACTATTTTGTCGAACAGAgtggt                                            >  1:2326478/1‑51 (MQ=255)
   gAAGAGCCGCGCTACCTGGCGCTGACGAACTATTTTGTCGAACAGAgtggt                                            >  1:6734160/1‑51 (MQ=255)
   gAAGAGCCGCGCTACCTGGCGCTGACGAACTATTTTGTCGAACAGAgtggt                                            >  1:3318208/1‑51 (MQ=255)
    aaGAGCCGCGCTACCTGGCGCTGACGAACTATTTTGTCGAACAGAgtggtg                                           >  1:4967872/1‑51 (MQ=255)
      gagCCGCGCTACCTGGCGCTGACGAACTATTTTGTCGAACAGAGTGGTgcg                                         >  1:7650639/1‑51 (MQ=255)
      gagCCGCGCTACCTGGCGCTGACGAACTATTTTGTCGAACAGAGTGGTgcg                                         >  1:9029223/1‑51 (MQ=255)
       agCCGCGCTACCTGGCGCTGACGAACTATTTTGTCGAACAGAGTGGTgcgc                                        <  1:5052788/51‑1 (MQ=255)
       agCCGCGCTACCTGGCGCTGACGAACTATTTTGTCGAACAGAGTGGTgcgc                                        <  1:7486127/51‑1 (MQ=255)
        gCCGCGCTACCTGGCGCTGACGAACTATTTTGTCGAACAGAGTGGTGCGCa                                       >  1:7680616/1‑51 (MQ=255)
        gCCGCGCTACCTGGCGCTGACGAACTATTTTGTCGAACAGAGTGGTGCGCa                                       <  1:8642655/51‑1 (MQ=255)
        gCCGCGCTACCTGGCGCTGACGAACTATTTTGTCGAACAGAGTGGTGCGCa                                       >  1:1406398/1‑51 (MQ=255)
         ccGCGCTACCTGGCGCTGACGAACTATTTTGTCGAACAGAGTGGTGCGCaa                                      >  1:5195985/1‑51 (MQ=255)
         ccGCGCTACCTGGCGCTGACGAACTATTTTGTCGAACAGAGTGGTGCGCaa                                      >  1:4415060/1‑51 (MQ=255)
          cgcgCTACCTGGCGCTGACGAACTATTTTGTCGAACAGAgtggtg                                           <  1:10742335/45‑1 (MQ=255)
          cgcgCTACCTGGCGCTGACGAACTATTTTGTCGAACAGAgtggt                                            <  1:10417137/44‑1 (MQ=255)
          cgcgCTACCTGGCGCTGACGAACTATTTTGTCGAACAGAGTGGTgc                                          <  1:8082911/46‑1 (MQ=255)
          cgcgCTACCTGGCGCTGACGAACTATTTTGTCGAACAGAGTGGTgc                                          <  1:4524136/46‑1 (MQ=255)
          cgcgCTACCTGGCGCTGACGAACTATTTTGTCGAACAGAGTGGTGCGCAAc                                     >  1:4321448/1‑51 (MQ=255)
           gcgcTACCTGGCGCTGACGAACTATTTTGTCGAACAGAGTGGTGCGCAAcc                                    >  1:9841943/1‑51 (MQ=255)
           gcgcTACCTGGCGCTGACGAACTATTTTGTCGAACAGAGTGGTGCGCAAcc                                    <  1:4657676/51‑1 (MQ=255)
           gcgcTACCTGGCGCTGACGAACTATTTTGTCGAACAGAGTGGTGCGCAAcc                                    >  1:5988254/1‑51 (MQ=255)
            cgcTACCTGGCGCTGACGAACTATTTTGTCGAACAGAGTGGTGCGCAACCg                                   >  1:4662497/1‑51 (MQ=255)
             gcTACCTGGCGCTGACGAACTATTTTGTCGAACAGAGTGGTGCGCAACCGc                                  >  1:5490/1‑51 (MQ=255)
             gcTACCTGGCGCTGACGAACTATTTTGTCGAACAGAGTGGTGCGCAACCGc                                  >  1:8663601/1‑51 (MQ=255)
             gcTACCTGGCGCTGACGAACTATTTTGTCGAACAGAGTGGTGCGCAACCGc                                  >  1:3182075/1‑51 (MQ=255)
              cTACCTGGCGCTGACGAACTATTTTGTCGAACAGAGTGGTGCGCAACCGCa                                 <  1:4769457/51‑1 (MQ=255)
               tACCTGGCGCTGACGAACTATTTTGTCGAACAGAGTGGTGCGCAACCGCAc                                >  1:1962488/1‑51 (MQ=255)
               tACCTGGCGCTGACGAACTATTTTGTCGAACAGAGTGGTGCGCAACCGCAc                                >  1:4823765/1‑51 (MQ=255)
               tACCTGGCGCTGACGAACTATTTTGTCGAACAGAGTGGTGCGCAACCGCAc                                >  1:5654245/1‑51 (MQ=255)
               tACCTGGCGCTGACGAACTATTTTGTCGAACAGAGTGGTGCGCAACCGCAc                                >  1:665723/1‑51 (MQ=255)
               tACCTGGCGCTGACGAACTATTTTGTCGAACAGAGTGGTGCGCAACCGCAc                                >  1:7411239/1‑51 (MQ=255)
                aCCTGGCGCTGACGAACTATTTTGTCGAACAGAGTGGTGCGCAACCGCACt                               >  1:347797/1‑51 (MQ=255)
                aCCTGGCGCTGACGAACTATTTTGTCGAACAGAGTGGTGCGCAACCGCACt                               >  1:3869315/1‑51 (MQ=255)
                  cTGGCGCTGACGAACTATTTTGTCGAACAGAGTGGTGCGCAACCGCACTAt                             <  1:6235125/51‑1 (MQ=255)
                  cTGGCGCTGACGAACTATTTTGTCGAACAGAGTGGTGCGCAACCGCACTAt                             <  1:5017084/51‑1 (MQ=255)
                   tGGCGCTGACGAACTATTTTGTCGAACAGAGTGGTGCGCAACCGCACTAtt                            <  1:3765743/51‑1 (MQ=255)
                   tGGCGCTGACGAACTATTTTGTCGAACAGAGTGGTGCGCAACCGCACTAtt                            >  1:7722861/1‑51 (MQ=255)
                   tGGCGCTGACGAACTATTTTGTCGAACAGAGTGGTGCGCAACCGCACTAtt                            <  1:4690899/51‑1 (MQ=255)
                    ggCGCTGACGAACTATTTTGTCGAACAGAGTGGTGCGCAACCGCACTATTa                           <  1:4352963/51‑1 (MQ=255)
                    ggCGCTGACGAACTATTTTGTCGAACAGAGTGGTGCGCAACCGCACTATTa                           <  1:8654363/51‑1 (MQ=255)
                    ggCGCTGACGAACTATTTTGTCGAACAGAGTGGTGCGCAACCGCACTATTa                           >  1:6229280/1‑51 (MQ=255)
                    ggCGCTGACGAACTATTTTGTCGAACAGAGTGGTGCGCAACCGCACTATTa                           >  1:3823840/1‑51 (MQ=255)
                    ggCGCTGACGAACTATTTTGTCGAACAGAGTGGTGCGCAACCGCACTATTa                           <  1:521381/51‑1 (MQ=255)
                     gcgcTGACGAACTATTTTGTCGAACAGAGTGGTGCGCAACCGCACTATTAc                          <  1:6841752/51‑1 (MQ=255)
                      cgcTGACGAACTATTTTGTCGAACAGAGTGGTGCGCAACCTCACTATTACg                         <  1:5024383/51‑1 (MQ=255)
                      cgcTGACGAACTATTTTGTCGAACAGAGTGGTGCGCAACCGCACTATTACg                         <  1:1868821/51‑1 (MQ=255)
                      cgcTGACGAACTATTTTGTCGAACAGAGTGGTGCGCAACCGCACTATTACg                         <  1:10833143/51‑1 (MQ=255)
                      cgcTGACGAACTATTTTGTCGAACAGAGTGGTGCGCAACCGCACTATTACg                         <  1:6911968/51‑1 (MQ=255)
                      cgcTGACGAACTATTTTGTCGAACAGAGTGGTGCGCAACCGCACTATTACg                         <  1:9267492/51‑1 (MQ=255)
                      cgcTGACGAACTATTTTGTCGAACAGAGTGGTGCGCAACCGCACTATTACg                         <  1:8340708/51‑1 (MQ=255)
                      cgcTGACGAACTATTTTGTCGAACAGAGTGGTGCGCAACCGCACTATTACg                         <  1:1936854/51‑1 (MQ=255)
                        cTGACGAACTATTTTGTCGAACAGAGTGGTGCGCAACCGCACTATTACGAc                       >  1:665472/1‑51 (MQ=255)
                        cTGACGAACTATTTTGTCGAACAGAGTGGTGCGCAACCGCACTATTACGAc                       <  1:8192617/51‑1 (MQ=255)
                         tGACGAACTATTTTGTCGAACAGAGTGGTGCGCAACCGCACTATTACGAcc                      >  1:6308743/1‑51 (MQ=255)
                         tGACGAACTATTTTGTCGAACAGAGTGGTGCGCAACCGCACTATTACGAcc                      <  1:7324903/51‑1 (MQ=255)
                          gACGAACTATTTTGTCGAACAGAGTGGTGCGCAACCGCACTATTACGACCa                     >  1:6208241/1‑51 (MQ=255)
                          gACGAACTATTTTGTCGAACAGAGTGGTGCGCAACCGCACTATTACGACCa                     <  1:435686/51‑1 (MQ=255)
                          gACGAACTATTTTGTCGAACAGAGTGGTGCGCAACCGCACTATTACGACCa                     >  1:2212751/1‑51 (MQ=255)
                           aCGAACTATTTTGTCGAACAGAGTGGTGCGCAACCGCACTATTACGACCaa                    <  1:9246970/51‑1 (MQ=255)
                           aCGAACTATTTTGTCGAACAGAGTGGTGCGCAACCGCACTATTACGACCaa                    <  1:211692/51‑1 (MQ=255)
                            cGAACTATTTTGTCGAACAGAGTGGTGCGCAACCGCACTATTACGACCAAg                   <  1:2171688/51‑1 (MQ=255)
                            cGAACTATTTTGTCGAACAGAGTGGTGCGCAACCGCACTATTACGACCAAg                   <  1:6934808/51‑1 (MQ=255)
                            cGAACTATTTTGTCGAACAGAGTGGTGCGCAACCGCACTATTACGACCAAg                   >  1:1929677/1‑51 (MQ=255)
                             gAACTATTTTGTCGAACAGAGTGGTGCGCAACCGCACTATTACGACCAAGa                  >  1:8100903/1‑51 (MQ=255)
                              aaCTATTTTGTCGAACAGAGTGGTGCGCAACCGCACTATTACGACCAAGaa                 >  1:7908401/1‑51 (MQ=255)
                               aCTATTTTGTCGAACAGAGTGGTGCGCAACCGCCCTATTACGACCAAGAat                >  1:8822054/1‑51 (MQ=255)
                               aCTATTTTGTCGAACAGAGTGGTGCGCAACCGCACTATTACGACCAAGAat                <  1:5247957/51‑1 (MQ=255)
                               aCTATTTTGTCGAACAGAGTGGTGCGCAACCGCACTATTACGACCAAGAat                >  1:8559889/1‑51 (MQ=255)
                                cTATTTTGTCGAACAGAGTGGTGCGCAACCGCACTATTACGACCAAGAata               >  1:1047879/1‑51 (MQ=255)
                                cTATTTTGTCGAACAGAGTGGTGCGCAACCGCACTATTACGACCAAGAata               >  1:26901/1‑51 (MQ=255)
                                cTATTTTGTCGAACAGAGTGGTGCGCAACCGCACTATTACGACCAAGAata               <  1:2349517/51‑1 (MQ=255)
                                 tATTTTGTTGAACAGAGTGGTGCGCAACCGCACTATTACGACCAAGAatat              <  1:4578621/51‑1 (MQ=255)
                                 tATTTTGTCGAACAGAGTGGTGCGCAACCGCACTATTACGACCAAGAatat              <  1:2069183/51‑1 (MQ=255)
                                 tATTTTGTCGAACAGAGTGGTGCGCAACCGCACTATTACGACCAAGAatat              >  1:7647819/1‑51 (MQ=255)
                                 tATTTTGTCGAACAGAGTGGTGCGCAACCGCACTATTACGACCAAGAatat              >  1:9704251/1‑51 (MQ=255)
                                 tATTTTGTCGAACAGAGTGGTGCGCAACCGCACTATTACGACCAAGAatat              >  1:525515/1‑51 (MQ=255)
                                 tATTTTGTCGAACAGAGTGGTGCGCAACCGCACTATTACGACCAAGAatat              <  1:4403494/51‑1 (MQ=255)
                                  aTTTTGTCGAACAGAGTGGTGCGCAACCGCACTATTACGACCAAGAATATg             <  1:10208946/51‑1 (MQ=255)
                                   ttttGTCGAACAGAGTGGTGCGCAACCGCACTATTACGACCAAGAATATGa            >  1:3800943/1‑51 (MQ=255)
                                     ttGTCGAACAGAGTGGTGCGCAACCGCACTATTACGACCAAGAATATGaaa          <  1:4077315/51‑1 (MQ=255)
                                      tGTCGAACAGAGTGGTGCGCAACCGCACTATTACGACCAAGAATATGaaaa         >  1:9235080/1‑51 (MQ=255)
                                      tGTCGAACAGAGTGGTGCGCAACCGCACTATTACGACCAAGAATATGaaaa         <  1:8565470/51‑1 (MQ=255)
                                      tGTCGAACAGAGTGGTGCGCAACCGCACTATTACGACCAAGAATATGaaaa         <  1:9058416/51‑1 (MQ=255)
                                        tCGAACAGAGTGGTGCGCAACCGCACTATTACGACCAAGAATATGAAAAgc       <  1:1103212/51‑1 (MQ=255)
                                        tCGAACAGAGTGGTGCGCAACCGCACTATTACGACCAAGAATATGAAAAgc       >  1:7078060/1‑51 (MQ=255)
                                         cGAACAGAGTGGTGCGCAACCGCACTATTACGACCAAGAATATGAAAAgcg      >  1:1939244/1‑51 (MQ=255)
                                          gAACAGAGTGGTGTGCAACCGCACTATTACGACCAAGAATATGAAAAgcgc     >  1:1805237/1‑51 (MQ=255)
                                           aaCAGAGTGGTGCGCAACCGCACTATTACGACCAAGAATATGAAAAGcgcg    <  1:2170652/51‑1 (MQ=255)
                                           aaCAGAGTGGTGCGCAACCGCACTATTACGACCAAGAATATGAAAAGcgcg    <  1:5589974/51‑1 (MQ=255)
                                            aCAGAGTGGTGCGCAACCGCACTATTACGACCAAGAATATGAAAAGCGCgg   >  1:6288098/1‑51 (MQ=255)
                                             cAGAGTGGTGCGCAACCGCACTATTACGACCAAGAATATGAAAAGCGCggg  >  1:698012/1‑51 (MQ=255)
                                             cAGAGTGGTGCGCAACCGCACTATTACGACCAAGAATATGAAAAGCGCggg  >  1:7535641/1‑51 (MQ=255)
                                             cAGAGTGGTGCGCAACCGCACTATTACGACCAAGAATATGAAAAGCGCggg  >  1:8540643/1‑51 (MQ=255)
                                                |                                               
ACTGAAGAGCCGCGCTACCTGGCGCTGACGAACTATTTTGTCGAACAGCGTGGTGCGCAACCGCACTATTACGACCAAGAATATGAAAAGCGCGGG  >  REL606/3684318‑3684413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: