| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | REL606 | 3,176,730 | T→C | 100% | Y349C (TAC→TGC) | uxaC ← | glucuronate isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,176,730 | 0 | T | C | 99.1% | 425.4 / ‑4.8 | 112 | Y349C (TAC→TGC) | uxaC | glucuronate isomerase |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (52/59); total (52/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
Only 100 of 120 total aligned reads displayed.
TTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTAGAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGC > REL606/3176682‑3176777 | ttACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGcagcag < 1:2513683/51‑1 (MQ=255)ttACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGcagcag > 1:5597554/1‑51 (MQ=255)ttACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGcagcag < 1:1619420/51‑1 (MQ=255) tACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGCaga < 1:5095983/51‑1 (MQ=255) tACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGCaga < 1:10627767/51‑1 (MQ=255) tACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGCaga < 1:2637289/51‑1 (MQ=255) aCCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGCagag < 1:6428291/51‑1 (MQ=255) ccGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGCAgaga < 1:2914545/51‑1 (MQ=255) cGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGCAGAGaa < 1:10747740/51‑1 (MQ=255) cGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGCAGAGaa < 1:3169263/51‑1 (MQ=255) cGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGCAGAGaa < 1:7447507/51‑1 (MQ=255) gATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGCAGAGAAt < 1:1765262/51‑1 (MQ=255) gATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGCAGAGAAt > 1:3980043/1‑51 (MQ=255) gATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGCAGAGAAt < 1:5166842/51‑1 (MQ=255) gATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGCAGAGAAt < 1:6452193/51‑1 (MQ=255) aTCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGCAGAGAATg > 1:10366350/1‑51 (MQ=255) aTCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGCAGAGAATg < 1:9325173/51‑1 (MQ=255) tCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGCAGAGAATgg > 1:2222926/1‑51 (MQ=255) tCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGCAGAGAATgg < 1:3389183/51‑1 (MQ=255) cATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGCAGAGAATGGt > 1:1171104/1‑51 (MQ=255) aTGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGCAGAGAATGGtt > 1:3892888/1‑51 (MQ=255) aTGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGCAGAGAATGGtt < 1:6815832/51‑1 (MQ=255) aTGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGCAGAGAATGGtt < 1:5993719/51‑1 (MQ=255) tGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGCAGAGAATGGttt > 1:10190399/1‑51 (MQ=255) tGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGCAGAGAATGGttt < 1:4500008/51‑1 (MQ=255) ggTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGCAGAGAATGGtttt > 1:2102316/1‑51 (MQ=255) ggTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGCAGAGAATGGtttt < 1:66350/51‑1 (MQ=255) gTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGCAGAGAATGGTTTTc < 1:3229702/51‑1 (MQ=255) gTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGCAGAGAATGGTTTTc > 1:3483954/1‑51 (MQ=255) gTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGCAGAGAATGGTTTTc < 1:5284360/51‑1 (MQ=255) gTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGCAGAGAATGGTTTTc > 1:1412901/1‑51 (MQ=255) gTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGCAGAGAATGGTTTTc < 1:6722022/51‑1 (MQ=255) gCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGCAGAGAATGGTTTTCgg < 1:8438161/51‑1 (MQ=255) cccAGCACTTCGTTATCGCGCGGGTTCAGGCAGCAGAGAATGGTTTTCGgc > 1:6653531/1‑51 (MQ=255) cccAGCACTTCGTTATCGCGCGGGTTCAGGCAGCAGAGAATGGTTTTCGgc > 1:2361622/1‑51 (MQ=255) cccAGCACTTCGTTATCGCGCGGGTTCAGGCAGCAGAGAATGGTTTTCGgc > 1:7986450/1‑51 (MQ=255) cccAGCACTTCGTTATCGCGCGGGTTCAGGCAGCAGAGAATGGTTTTCGgc > 1:1309288/1‑51 (MQ=255) ccAGCACTTCGTTATCGCGCGGGTTCAGGCAGCTGAGAATGGTTTTCGgca < 1:5545965/51‑1 (MQ=38) ccAGCACTTCGTTATCGCGCGGGTTCAGGCAGCAGAGAATGGTTTTCGgca > 1:6668000/1‑51 (MQ=255) ccAGCACTTCGTTATCGCGCGGGTTCAGGCAGCAGAGAATGGTTTTCGgca > 1:4232747/1‑51 (MQ=255) ccAGCACTTCGTTATCGCGCGGGTTCAGGCAGCAGAGAATGGTTTTCGgca > 1:10473553/1‑51 (MQ=255) cAGCACTTCGTTATCGCGCGGGTTCAGGCAGCAGAGAATGGTTTTCGgcag > 1:10377504/1‑51 (MQ=255) aGCACTTCGTTATCGCGCGGGTTCAGGCAGCAGAGAATGGTTTTCGgcagc > 1:7550984/1‑51 (MQ=255) gCACTTCGTTATCGCGCGGGTTCAGGCAGCAGAGAATGGTTTTCGgcagca < 1:4745076/51‑1 (MQ=255) gCACTTCGTTATCGCGCGGGTTCAGGCAGCAGAGAATGGTTTTCGgcagca < 1:9172133/51‑1 (MQ=255) gCACTTCGTTATCGCGCGGGTTCAGGCAGCAGAGAATGGTTTTCGgca > 1:5007329/1‑48 (MQ=255) cACTTCGTTATCGCGCGGGTTCAGGCAGCAGAGAATGGTTTTCGGcagcag > 1:3188497/1‑51 (MQ=255) aCTTCGTTATCGCGCGGGTTCAGGCAGCAGAGAATGGTTTTCGGCAGCAgg > 1:9467692/1‑51 (MQ=255) aCTTCGTTATCGCGCGGGTTCAGGCAGCAGAGAATGGTTTTCGGCAGCAgg < 1:6474601/51‑1 (MQ=255) aCTTCGTTATCGCGCGGGTTCAGGCAGCAGAGAATGGTTTTCGGCAGCAgg < 1:5448173/51‑1 (MQ=255) aCTTCGTTATCGCGCGGGTTCAGGCAGCAGAGAATGGTTTTCGGCAGCAgg > 1:4408224/1‑51 (MQ=255) aCTTCGTTATCGCGCGGGTTCAGGCAGCAGAGAATGGTTTTCGGCAGCAgg < 1:2764111/51‑1 (MQ=255) aCTTCGTTATCGCGCGGGTTCAGGCAGCAGAGAATGGTTTTCGGCAGCAgg > 1:1042065/1‑51 (MQ=255) cTTCGTTATCGCGCGGGTTCAGGCAGCAGAGAATGGTTTTCGGCAGCAGGt < 1:8762335/51‑1 (MQ=255) cTTCGTTATCGCGCGGGTTCAGGCAGCAGAGAATGGTTTTCGGCAGCAGGt > 1:7329677/1‑51 (MQ=255) cTTCGTTATCGCGCGGGTTCAGGCAGCAGAGAATGGTTTTCGGCAGCAGGt > 1:2244285/1‑51 (MQ=255) ttCGTTATCGCGCGGGTTCAGGCAGCAGAGAATGGTTTTCGGCAGCAgg > 1:5339435/1‑49 (MQ=255) ttCGTTATCGCGCGGGTTCAGGCAGCAGAGAATGGTTTTCGGCAGCAGGtt < 1:2257041/51‑1 (MQ=255) tCGTTATCGCGCGGGTTCAGGCAGCAGAGAATGGTTTTCGGcagcag < 1:582316/47‑1 (MQ=255) cGTTATCGCGCGGGTTCAGGCAGCAGAGAATGGTTTTCGGCAGCAgg > 1:2566394/1‑47 (MQ=255) cGTTATCGCGCGGGTTCAGGCAGCAGAGAATGGTTTTCGGCAGCAGGtttt < 1:9347112/51‑1 (MQ=255) ttATCGCGCGGGTTCAGGCAGCAGAGAATGGTTTTCGGCAGCAGGTTttct > 1:4255353/1‑51 (MQ=255) tATCGCGCGGGTTCAGGCAGCGGAGAATGGTTTTCGGCAGCAGGTTttctt > 1:8274689/1‑51 (MQ=255) tATCGCGCGGGTTCAGGCAGCAGAGAATGGTTTTCGGCAGCAGGTTttctt > 1:10223250/1‑51 (MQ=255) tATCGCGCGGGTTCAGGCAGCAGAGAATGGTTTTCGGCAGCAGGTTttctt > 1:6323834/1‑51 (MQ=255) tCGCGCGGGTTCAGGCAGCAGAGAATGGTTTTCGGCAGCAGGTTTTCTTCa < 1:10844456/51‑1 (MQ=255) cgcgcgGGTTCAGGCAGCAGAGAATGGTTTTCGGCAGCAGGTTttct < 1:5197235/47‑1 (MQ=255) cgcgcgGGTTCAGGCAGCAGAGAATGGTTTTCGGCAGCAGGTTTTCTTCAt > 1:8083999/1‑51 (MQ=255) cgcgcgGGTTCAGGCAGCAGAGAATGGTTTTCGGCAGCAGGTTTTCTTCAt < 1:7873785/51‑1 (MQ=255) cgcgcgGGTTCAGGCAGCAGAGAATGGTTTTCGGCAGCAGGTTTTCTTCAt < 1:3219023/51‑1 (MQ=255) gcgcgGGTTCAGGCAGCAGAGAATGGTTTTCGGCAGCAGGTTTTCTTCAtt > 1:3705320/1‑51 (MQ=255) cgcgGGTTCAGGCAGCAGAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTc > 1:3286005/1‑51 (MQ=255) cgcgGGTTCAGGCAGCAGAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTc < 1:2920526/51‑1 (MQ=255) gcgGGTTCAGGCAGCAGAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCt < 1:1164899/51‑1 (MQ=255) cgGGTTCAGGCAGCAGAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCtg > 1:3892246/1‑51 (MQ=255) cgGGTTCAGGCAGCAGAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCtg > 1:10914213/1‑51 (MQ=255) gggTTCAGGCAGCAGAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCtgc < 1:3035602/51‑1 (MQ=255) gggTTCAGGCAGCAGAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCtgc > 1:391559/1‑51 (MQ=255) gggTTCAGGCAGCAGAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCtgc > 1:4550342/1‑51 (MQ=255) gggTTCAGGCAGCAGAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCtgc < 1:3592645/51‑1 (MQ=255) ggTTCAGGCAGCAGAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCtgct > 1:1664651/1‑51 (MQ=255) gTTCAGGCAGCAGAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCtgctt < 1:9364744/51‑1 (MQ=255) gTTCAGGCAGCAGAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCtgctt < 1:101007/51‑1 (MQ=255) gTTCAGGCAGCAGAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCtgctt < 1:5712666/51‑1 (MQ=255) ttCAGGCAGCAGAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCtgcttg < 1:153430/51‑1 (MQ=255) ttCAGGCAGCAGAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCtgcttg < 1:8027009/51‑1 (MQ=255) ttCAGGCAGCAGAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCtgcttg > 1:10765616/1‑51 (MQ=255) tCAGGCAGCAGAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCtgcttgc > 1:3324381/1‑51 (MQ=255) tCAGGCAGCAGAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCtgcttgc < 1:6388064/51‑1 (MQ=255) tCAGGCAGCAGAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCtgcttgc > 1:404963/1‑51 (MQ=255) tCAGGCAGCAGAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCtgcttgc < 1:2038108/51‑1 (MQ=255) cAGGCAGCAGAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCtgcttgct > 1:7281051/1‑51 (MQ=255) aGGCAGCAGAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTc < 1:234703/51‑1 (MQ=255) aGGCAGCAGAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTc < 1:7758845/51‑1 (MQ=255) ggCAGCAGAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCa < 1:8307856/51‑1 (MQ=255) gcagcaGAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAg > 1:2727651/1‑51 (MQ=255) gcagcaGAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAg < 1:7403572/51‑1 (MQ=255) gcagcaGAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAg < 1:8663418/51‑1 (MQ=255) cagcagAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGc > 1:4273599/1‑51 (MQ=255) cagcagAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGc > 1:4274854/1‑51 (MQ=255) | TTACCGATCATGGTGCCCAGCACTTCGTTATCGCGCGGGTTCAGGCAGTAGAGAATGGTTTTCGGCAGCAGGTTTTCTTCATTCTGCTTGCTCAGC > REL606/3176682‑3176777 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |