| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | REL606 | 962,002 | G→A | 100% | intergenic (+13/+22) | dmsC → / ← ycaC | dimethyl sulfoxide reductase, anaerobic, subunit C/predicted hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 962,002 | 0 | G | A | 97.7% | 288.2 / ‑2.0 | 87 | intergenic (+13/+22) | dmsC/ycaC | dimethyl sulfoxide reductase, anaerobic, subunit C/predicted hydrolase |
| Reads supporting (aligned to +/- strand): ref base G (2/0); new base A (41/44); total (43/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.41e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTGCATATGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGGGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTAACGTGTCATA > REL606/961954‑962050 | ttGCATATGACCGCGGGGATGGCCGTCGCAAGCTAATCATAACAACCgagg < 1:8418896/51‑1 (MQ=255) tGCATATGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGt > 1:5044377/1‑51 (MQ=255) tGCATATGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGt > 1:10463809/1‑51 (MQ=255) gCATATGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGtt > 1:3274011/1‑51 (MQ=255) gCATATGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGtt > 1:30059/1‑51 (MQ=255) gCATATGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGtt > 1:1972588/1‑51 (MQ=255) cATATGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGttt > 1:7795349/1‑51 (MQ=255) atatGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCgagg > 1:4000365/1‑47 (MQ=255) atatGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTc < 1:8772783/51‑1 (MQ=255) atatGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTc < 1:5724170/51‑1 (MQ=255) atatGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTc < 1:3740062/51‑1 (MQ=255) atatGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTc < 1:1697988/51‑1 (MQ=255) atatGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTc < 1:6540015/51‑1 (MQ=255) tatGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCg < 1:9198556/51‑1 (MQ=255) tatGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCg > 1:96659/1‑51 (MQ=255) tatGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCg < 1:87216/51‑1 (MQ=255) atGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCgg > 1:2172736/1‑51 (MQ=255) tGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCgac > 1:9095625/1‑49 (MQ=255) tGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGc < 1:9457884/51‑1 (MQ=255) gACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGcc > 1:7311648/1‑51 (MQ=255) gACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGcc < 1:8227595/51‑1 (MQ=255) aCCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGccc > 1:7015460/1‑51 (MQ=255) ccGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGcccc < 1:10015798/51‑1 (MQ=255) ccGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGcccc > 1:3828477/1‑51 (MQ=255) ccGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGcccc < 1:8912385/51‑1 (MQ=255) ccGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGcccc < 1:5050091/51‑1 (MQ=255) ccGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGcccc < 1:1756698/51‑1 (MQ=255) ccGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGcccc < 1:5632499/51‑1 (MQ=255) cGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCg < 1:5352987/51‑1 (MQ=255) gTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGcccc > 1:9963712/1‑49 (MQ=255) gTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCgg > 1:3026001/1‑51 (MQ=255) gTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCgg > 1:3557461/1‑51 (MQ=255) tGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGt > 1:9583625/1‑51 (MQ=255) tGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGt > 1:7909919/1‑51 (MQ=255) tGGGGATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGt < 1:4571668/51‑1 (MQ=255) ggggATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGtt < 1:3844897/51‑1 (MQ=255) ggggATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGtt < 1:3016394/51‑1 (MQ=255) ggggATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGtt < 1:1761851/51‑1 (MQ=255) ggggATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGtt > 1:8657730/1‑51 (MQ=255) gggATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTtc < 1:4256136/51‑1 (MQ=255) ggATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTtct > 1:4455197/1‑51 (MQ=255) ggATGGCCATCGCAAGCTAATCATAGCAACCGAGGTTTCGGCCCCGGTtct < 1:4923929/51‑1 (MQ=38) gATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTtctc < 1:7216070/51‑1 (MQ=255) gATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTtctc > 1:6429786/1‑51 (MQ=255) gATGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTtctc > 1:6414108/1‑51 (MQ=255) aTGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTctct < 1:479674/51‑1 (MQ=255) tGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCtt > 1:3181795/1‑51 (MQ=255) tGGCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCtt > 1:9043800/1‑51 (MQ=255) ggCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCttt < 1:2409930/51‑1 (MQ=255) ggCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCttt < 1:9454076/51‑1 (MQ=255) gCCGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCtttt < 1:4975094/51‑1 (MQ=255) cGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCtt < 1:1832647/47‑1 (MQ=255) cGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTAt < 1:5101912/51‑1 (MQ=255) cGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTAt > 1:3559913/1‑51 (MQ=255) cGTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTAt > 1:4670901/1‑51 (MQ=255) gTCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTAtt < 1:8525123/51‑1 (MQ=255) tCGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTAttt > 1:3760343/1‑51 (MQ=255) cGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTc < 1:4585732/51‑1 (MQ=255) cGCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTc > 1:5044324/1‑51 (MQ=255) gCAAGCTAATCATAACAACCGGGGTTTCGGCCCCGGTTCTCTTTTATTTCt > 1:3991316/1‑51 (MQ=255) gCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCt > 1:2744602/1‑51 (MQ=255) gCAAGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCt < 1:7420611/51‑1 (MQ=255) aaGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGc < 1:7061107/51‑1 (MQ=255) aaGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGc > 1:7564143/1‑51 (MQ=255) aaGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGc < 1:1430990/51‑1 (MQ=255) aaGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGc > 1:914605/1‑51 (MQ=255) aaGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGc > 1:3008208/1‑51 (MQ=255) aGCTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCt < 1:8687066/51‑1 (MQ=255) cTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTc < 1:5154748/51‑1 (MQ=255) cTAATCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTc < 1:4582127/51‑1 (MQ=255) aaTCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGt < 1:7072939/51‑1 (MQ=255) aaTCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGt > 1:1768072/1‑51 (MQ=255) aaTCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGt > 1:9289120/1‑51 (MQ=255) aTCATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGtt < 1:7749093/51‑1 (MQ=255) cATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTaa < 1:9331407/51‑1 (MQ=255) cATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTaa > 1:6012394/1‑51 (MQ=255) cATAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTaa < 1:5200165/51‑1 (MQ=255) aTAACAACCGGGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTAAc > 1:7241579/1‑51 (MQ=255) aTAACAACCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTAAc < 1:2105095/51‑1 (MQ=255) aacaacCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTaa < 1:3680932/48‑1 (MQ=255) aacaacCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTAACgt > 1:253824/1‑51 (MQ=255) acaacCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTAACgtg > 1:3577452/1‑51 (MQ=255) cttatgggTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTAACGTGTc < 1:3578939/46‑1 (MQ=255) aacCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTAACGTGTc > 1:3069572/1‑51 (MQ=255) aacCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTAACGTGTc > 1:10860890/1‑51 (MQ=255) aacCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTAACGTGTc > 1:5200482/1‑51 (MQ=255) aacCGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTAACGTGTc > 1:7870124/1‑51 (MQ=255) ccGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTAACGTGTCa < 1:2418982/50‑1 (MQ=255) ccGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTAACGTGTCAt > 1:7104493/1‑51 (MQ=255) ccGAGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTAACGTGTCAt < 1:10467784/51‑1 (MQ=255) atgggTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTAACGTGTCATa > 1:8971526/3‑51 (MQ=255) | TTGCATATGACCGTGGGGATGGCCGTCGCAAGCTAATCATAACAACCGGGGTTTCGGCCCCGGTTCTCTTTTATTTCTGCTTCGTTAACGTGTCATA > REL606/961954‑962050 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |