Predicted mutation
evidence seq id position mutation freq annotation gene description
RA REL606 761,693 T→G 100% noncoding (47/76 nt) valT → tRNA‑Val

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL606761,6930TG100.0% 96.4 / NA 31noncoding (47/76 nt)valTtRNA‑Val
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (18/13);  total (18/13)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGT  >  REL606/761645‑761740
                                                |                                               
ttGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGGcg                                               <  1:5705225/51‑1 (MQ=9)
                             cTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCaccacc                  >  1:4556850/1‑51 (MQ=9)
                             cTCCCTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCaccacc                  <  1:5097971/51‑1 (MQ=9)
                               cccTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCgg                <  1:2532815/51‑1 (MQ=255)
                                ccTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCggg               >  1:5788087/1‑51 (MQ=255)
                                ccTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCggg               <  1:2959045/51‑1 (MQ=255)
                                ccTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCggg               >  1:2176831/1‑51 (MQ=255)
                                ccTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCggg               <  1:2417319/51‑1 (MQ=255)
                                ccTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCggg               >  1:4518548/1‑51 (MQ=255)
                                 cTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGt              <  1:9162662/51‑1 (MQ=255)
                                 cTTACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGt              <  1:1563778/51‑1 (MQ=255)
                                  ttACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCGCCCACCACCGGGTc             <  1:6537703/51‑1 (MQ=14)
                                  ttACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCggg               >  1:1609026/1‑49 (MQ=255)
                                  ttACAAGGAGGGGGGCGGCGGTTCGATCCCATCATCACCCACCACCGGGTc             >  1:2638330/1‑51 (MQ=14)
                                   tACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTc             >  1:5741410/1‑50 (MQ=255)
                                   tACAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCg            >  1:3261969/1‑51 (MQ=255)
                                   tACAAGGAGGGGGGCAGCGGTTCGATCCCGTCATCACCCaccacc                  >  1:1282872/1‑45 (MQ=11)
                                     cAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGtt          <  1:3383515/51‑1 (MQ=255)
                                     cAAGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGtt          >  1:2521797/1‑51 (MQ=255)
                                      aaGGAGGGGGGCGGCGGTTTGATCCCGTCATCACCCACCACCGGGTCGTTa         >  1:6988787/1‑51 (MQ=14)
                                      aaGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCaccacc                  <  1:2127278/42‑1 (MQ=12)
                                      aaGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTa         <  1:5262567/51‑1 (MQ=255)
                                      aaGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTa         >  1:1368282/1‑51 (MQ=255)
                                      aaGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTa         >  1:889181/1‑51 (MQ=255)
                                      aaGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTa         >  1:864304/1‑51 (MQ=255)
                                      aaGGAGGGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTa         >  1:6059958/1‑51 (MQ=255)
                                        ggaggGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGc       >  1:2867317/1‑51 (MQ=255)
                                          aggGGGGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTc     <  1:606842/51‑1 (MQ=255)
                                           ggggggCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTc     <  1:5192031/50‑1 (MQ=255)
                                           ggggggCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCa    >  1:2167040/1‑51 (MQ=255)
                                           ggggggCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCa    <  1:73980/51‑1 (MQ=255)
                                           ggggggCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCa    <  1:5304973/51‑1 (MQ=255)
                                           ggggggCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCa    <  1:5362996/51‑1 (MQ=255)
                                           ggggggCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCa    <  1:6921055/51‑1 (MQ=255)
                                            ggggGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAg   >  1:8136415/1‑51 (MQ=255)
                                            ggggGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAg   <  1:9086209/51‑1 (MQ=255)
                                            ggggGCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAg   >  1:8106725/1‑51 (MQ=255)
                                             ggggCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGt  >  1:9505888/1‑51 (MQ=255)
                                                |                                               
TTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGT  >  REL606/761645‑761740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: