BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
MC JC JC	REL606	490,484	IS150 (+) Δ1 bp :: +CATT	100%	intergenic (‑37/+24)	ybbN ← / ← ybbO	predicted thioredoxin domain‑containing protein/short chain dehydrogenase

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	REL606	490484	490484	1	119 [0]	[0] 143	ybbN/ybbO	predicted thioredoxin domain‑containing protein/short chain dehydrogenase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 490483	0 (0.000)	83 (0.870)	53/80	NT	100%	intergenic (‑36/+25)	ybbN/ybbO	predicted thioredoxin domain‑containing protein/short chain dehydrogenase
*	?	REL606	= 2775877	NA (NA)	83 (0.870)	53/80	NT	100%	noncoding (1/1443 nt)	IS150	repeat region
*	?	REL606	490485 =	0 (0.000)	99 (1.130) +AAT	51/74	NT	100%	intergenic (‑38/+23)	ybbN/ybbO	predicted thioredoxin domain‑containing protein/short chain dehydrogenase
*	?	REL606	= 590472	NA (NA)	99 (1.130) +AAT	51/74	NT	100%	intergenic (+27/‑71)	insK‑1/hokE	IS150 putative transposase; b3558_1/toxic polypeptide, small

Only 100 of 143 total aligned reads displayed.

TTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACG  >  REL606/490436‑490535

ctatatgcctcgtgtttaactgtccaactttttggggtcagtacacattgg                                                   >  1:2124492‑M1/50‑51 (MQ=255)

ctatatgcctcgtgtttaactgtccaactttttggggtcagtacacattgg                                                   <  1:8426630‑M1/2‑1 (MQ=255)

ctatatgcctcgtgtttaactgtccaactttttggggtcagtacacattgg                                                   >  1:7786652‑M1/50‑51 (MQ=255)

 tatatgcctcgtgtttaactgtccaactttttggggtcagtacacattggg                                                  >  1:7722311‑M1/49‑51 (MQ=255)

 tatatgcctcgtgtttaactgtccaactttttggggtcagtacacattggg                                                  <  1:8933530‑M1/3‑1 (MQ=255)

 tatatgcctcgtgtttaactgtccaactttttggggtcagtacacattggg                                                  <  1:9163259‑M1/3‑1 (MQ=255)

 tatatgcctcgtgtttaactgtccaactttttggggtcagtacacattggg                                                  <  1:8585498‑M1/3‑1 (MQ=255)

  atatgcctcgtgtttaactgtccaactttttggggtcagtacacattgggg                                                 <  1:4269252‑M1/4‑1 (MQ=255)

  atatgcctcgtgtttaactgtccaactttttggggtcagtacacattgggg                                                 <  1:9149450‑M1/4‑1 (MQ=255)

   tatgcctcgtgtttaactgtccaactttttggggtcagtacacattGGGGc                                                >  1:10616848‑M1/47‑51 (MQ=255)

   tatgcctcgtgtttaactgtccaactttttggggtcagtacacattGGGGc                                                >  1:8296507‑M1/47‑51 (MQ=255)

     tgcctcgtgtttaactgtccaactttttggggtcagtacacattGGGGCtt                                              >  1:4239035‑M1/45‑51 (MQ=255)

      gcctcgtgtttaactgtccaactttttggggtcagtacacattGGGGCTTa                                             <  1:9641294‑M1/8‑1 (MQ=255)

      gcctcgtgtttaactgtccaactttttggggtcagtacacattGGGGCTTa                                             >  1:7499799‑M1/44‑51 (MQ=255)

      gcctcgtgtttaactgtccaactttttggggtcagtacacattGGGGCTTa                                             >  1:6398612‑M1/44‑51 (MQ=255)

      gcctcgtgtttaactgtccaactttttggggtcagtacacattGGGGCTTa                                             >  1:2269535‑M1/44‑51 (MQ=255)

      gcctcgtgtttaactgtccaactttttggggtcagtacacattGGGGCTTa                                             <  1:9790238‑M1/8‑1 (MQ=255)

       cctcgtgtttaactgtccaactttttggggtcagtacacattGGGGCTTaa                                            >  1:5401252‑M1/43‑51 (MQ=255)

       cctcgtgtttaactgtccaactttttggggtcagtacacattGGGGCTTaa                                            <  1:6821140‑M1/9‑1 (MQ=255)

       cctcgtgtttaactgtccaactttttggggtcagtacacattGGGGCTTaa                                            <  1:3672636‑M1/9‑1 (MQ=255)

        ctcgtgtttaactgtccaactttttggggtcagtacacattGGGGCTTAAg                                           >  1:5934439‑M1/42‑51 (MQ=255)

        ctcgtgtttaactgtccaactttttggggtcagtacacattGGGGCTTAAg                                           <  1:1207700‑M1/10‑1 (MQ=255)

        ctcgtgtttaactgtccaactttttggggtcagtacacattGGGGCTTAAg                                           >  1:9203986‑M1/42‑51 (MQ=255)

        ctcgtgtttaactgtccaactttttggggtcagtacacattGGGGCTTAAg                                           <  1:8082326‑M1/10‑1 (MQ=255)

         tcgtgtttaactgtccaactttttggggtcagtacacattGGGGCTTAAgc                                          >  1:8456326‑M1/41‑51 (MQ=255)

         tcgtgtttaactgtccaactttttggggtcagtacacattGGGGCTTAAgc                                          <  1:9641164‑M1/11‑1 (MQ=255)

         tcgtgtttaactgtccaactttttggggtcagtacacattGGGGCTTAAgc                                          <  1:2510701‑M1/11‑1 (MQ=255)

          cgtgtttaactgtccaactttttggggtcagtacacattGGGGCTTAAgcg                                         <  1:6863231‑M1/12‑1 (MQ=255)

           gtgtttaactgtccaactttttggggtcagtacacattGGGGCTTAAgcgc                                        >  1:8880578‑M1/39‑51 (MQ=255)

           gtgtttaactgtccaactttttggggtcagtacacattGGGGCTTAAgcgc                                        <  1:580195‑M1/13‑1 (MQ=255)

            tgtttaactgtccaactttttggggtcagtacacattGGGGCTTAAgcgcg                                       >  1:25967‑M1/38‑51 (MQ=255)

            tgtttaactgtccaactttttggggtcagtacacattGGGGCTTAAgcgcg                                       <  1:8080060‑M1/14‑1 (MQ=255)

             gtttaactgtccaactttttggggtcagtacacattGGGGCTTAAgcgcgc                                      >  1:1003660‑M1/37‑51 (MQ=255)

             gtttaactgtccaactttttggggtcagtacacattGGGGCTTAAgcgcgc                                      <  1:3779000‑M1/15‑1 (MQ=255)

             gtttaactgtccaactttttggggtcagtacacattGGGGCTTAAgcgcgc                                      <  1:4487432‑M1/15‑1 (MQ=255)

             gtttaactgtccaactttttggggtcagtacacattGGGGCTTAAgcgcgc                                      >  1:4332795‑M1/37‑51 (MQ=255)

               ttaactgtccaactttttggggtcagtacacattGGGGCTTAAgcg                                         >  1:4895184‑M1/35‑46 (MQ=255)

                taactgtccaactttttggggtcagtacacattGGGGCTTAAGCGCGCGCt                                   <  1:2027046‑M1/18‑1 (MQ=255)

                 aactgtccaactttttggggtcagtacacattGGGGCTTAAGCGCGCGCtt                                  <  1:2780657‑M1/19‑1 (MQ=255)

                  actgtccaactttttggggtcagtacacattGGGGCTTAAGCGCGCGCTTc                                 >  1:1442235‑M1/32‑51 (MQ=255)

                  actgtccaactttttggggtcagtacacattGGGGCTTAAGCGCGCGCTTc                                 >  1:6364666‑M1/32‑51 (MQ=255)

                  actgtccaactttttggggtcagtacacattGGGGCTTAAGCGCGCGCTTc                                 >  1:9251749‑M1/32‑51 (MQ=255)

                  actgtccaactttttggggtcagtacacattGGGGCTTAAGCGCGCGCTTc                                 >  1:3099070‑M1/32‑51 (MQ=255)

                     gtccaactttttggggtcagtacacattGGGGCTTAAGCGCGCGCTTCAAc                              <  1:10338342‑M1/23‑1 (MQ=255)

                       ccaactttttggggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTc                            >  1:3645430‑M1/27‑51 (MQ=255)

                        caactttttggggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCa                           >  1:1932177‑M1/26‑51 (MQ=255)

                         aactttttggggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCAc                          <  1:173204‑M1/27‑1 (MQ=255)

                         aactttttggggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCAc                          <  1:6496282‑M1/27‑1 (MQ=255)

                         aactttttggggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCAc                          <  1:5822169‑M1/27‑1 (MQ=255)

                         aactttttggggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCAc                          <  1:4275553‑M1/27‑1 (MQ=255)

                           ctttttggggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCAccc                        <  1:4758722‑M1/29‑1 (MQ=255)

                           ctttttggggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCAccc                        <  1:7126378‑M1/29‑1 (MQ=255)

                            tttttggggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCAcccc                       <  1:6836304‑M1/30‑1 (MQ=255)

                             ttttggggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCt                      <  1:2368012‑M1/31‑1 (MQ=255)

                              tttggggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTg                     <  1:5640748‑M1/32‑1 (MQ=255)

                              tttggggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTg                     <  1:10537789‑M1/32‑1 (MQ=255)

                              tttggggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTg                     <  1:6498329‑M1/32‑1 (MQ=255)

                              tttggggtcagtacacaatGGGGCTTAAGCGCGCGCTTCAACTCACCCCTg                     >  1:6885335‑M1/20‑51 (MQ=255)

                               ttggggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGc                    <  1:8998031‑M1/33‑1 (MQ=255)

                               ttggggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGc                    >  1:5788684‑M1/19‑51 (MQ=255)

                                tggggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCa                   >  1:404386‑M1/18‑51 (MQ=255)

                                 ggggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCaa                  >  1:9490281‑M1/17‑51 (MQ=255)

                                 ggggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCaa                  <  1:1245550‑M1/35‑1 (MQ=255)

                                 ggggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCaa                  >  1:2018575‑M1/17‑51 (MQ=255)

                                 ggggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCaa                  >  1:4335306‑M1/17‑51 (MQ=255)

                                  gggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAat                 >  1:1872016‑M1/16‑51 (MQ=255)

                                  gggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAat                 <  1:2669388‑M1/36‑1 (MQ=255)

                                  gggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAat                 <  1:7160641‑M1/36‑1 (MQ=255)

                                   ggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAata                >  1:6180745‑M1/15‑51 (MQ=255)

                                   ggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAata                >  1:1962137‑M1/15‑51 (MQ=255)

                                   ggtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAata                >  1:5786429‑M1/15‑51 (MQ=255)

                                    gtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAatat               >  1:4807175‑M1/14‑51 (MQ=255)

                                    gtcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAatat               >  1:2355436‑M1/14‑51 (MQ=255)

                                     tcagtacacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATAtt              <  1:3106259‑M1/39‑1 (MQ=255)

                                      cagtacacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATAttt             <  1:5358736‑M1/40‑1 (MQ=255)

                                      cagtacacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATAttt             <  1:3493785‑M1/40‑1 (MQ=255)

                                      cagtacacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATAttt             <  1:1988575‑M1/40‑1 (MQ=255)

                                         tacacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGt          >  1:7690484‑M1/9‑51 (MQ=255)

                                         tacacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGt          <  1:10836098‑M1/43‑1 (MQ=255)

                                          acacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTc         >  1:4023743‑M1/8‑51 (MQ=255)

                                          acacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTc         >  1:7255014‑M1/8‑51 (MQ=255)

                                           cacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTcc        >  1:4006105‑M1/7‑51 (MQ=255)

                                           cacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTcc        >  1:6984962‑M1/7‑51 (MQ=255)

                                           cacattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTcc        >  1:3965707‑M1/7‑51 (MQ=255)

                                            acattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCa       <  1:3726405‑M1/46‑1 (MQ=255)

                                             cattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCAt      >  1:6203560‑M1/5‑51 (MQ=255)

                                             cattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCAt      <  1:3538462‑M1/47‑1 (MQ=255)

                                             cattGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCAt      >  1:8266418‑M1/5‑51 (MQ=255)

                                             cattGGGACTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCAt      <  1:6600874‑M1/47‑1 (MQ=255)

                                              attGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATc     >  1:2565287‑M1/4‑51 (MQ=255)

                                              attGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATc     <  1:877003‑M1/48‑1 (MQ=255)

                                              attGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATc     >  1:7628054‑M1/4‑51 (MQ=255)

                                              attGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATc     <  1:5105398‑M1/48‑1 (MQ=255)

                                               ttGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCa    >  1:3242664‑M1/3‑51 (MQ=255)

                                               ttGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCa    <  1:7079269‑M1/49‑1 (MQ=255)

                                               ttGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCa    >  1:5554917‑M1/3‑51 (MQ=255)

                                               ttGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCa    >  1:9979540‑M1/3‑51 (MQ=255)

                                                tGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCAc   >  1:10754561‑M1/2‑51 (MQ=255)

                                                tGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCAc   >  1:10371211‑M1/2‑51 (MQ=255)

                                                 ggggCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCAcg  <  1:4942657/51‑1 (MQ=255)

TTCTACGGACATGGAGTCGCTCTCTGTTGTCGATATTTTCTTTGACATGGGGGCTTAAGCGCGCGCTTCAACTCACCCCTGCAATATTTTGTCCATCACG  >  REL606/490436‑490535

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`